With the rapid development of science and technology, 5G technology will be widely used, and biosafety concerns about the effects of 5G radiofrequency radiation on health have been raised. Drosophila melanogaster was selected as the model organism for our study, in which a 3.5 GHz radiofrequency radiation (RF-EMR) environment was simulated at intensities of 0.1 W/m², 1 W/m², and 10 W/m². The activity of parent male and offspring (F1) male flies was measured using a Drosophila activity monitoring system under short-term and long-term 3.5 GHz RF-EMR exposure. Core genes associated with heat stress, the circadian clock and neurotransmitters were detected by QRT-PCR technology, and the contents of GABA and glutamate were detected by UPLC-MS. The results show that short-term RF-EMR exposure increased the activity level and reduced the sleep duration while long-term RF-EMR exposure reduced the activity level and increased the sleep duration of F1 male flies. Under long-term RF-EMR, the expression of heat stress response-related hsp22, hsp26 and hsp70 genes was increased, the expression of circadian clock-related per, cyc, clk, cry, and tim genes was altered, the content of GABA and glutamate was reduced, and the expression levels of synthesis, transport and receptor genes were altered. In conclusion, long-term RF-EMR exposure enhances the heat stress response of offspring flies and then affects the expression of circadian clock and neurotransmitter genes, which leads to decreased activity, prolonged sleep duration, and improved sleep quality.

A green approach using hydrogen peroxide (H₂O₂) to intensify the fuel properties of spent coffee grounds (SCGs) through torrefaction is developed in this study to minimize environmental pollution. Meanwhile, a neural network (NN) is used to minimize bulk density at different combinations of operating conditions to show the accurate and reliable model of NN (R² = 0.9994). The biochar produced from SCGs torrefied at temperatures of 200–300 °C, duration of 30–60 min, and H₂O₂ concentrations of 0–100 wt% is examined. The results reveal that the higher heating value (HHV) of biochar increases with rising temperature, duration, or H₂O₂ concentration, whereas the bulk density has an opposite trend. The HHV, ignition temperature, and bulk density of biochar from torrefaction at 230 °C for 30 min with a 100 wt% H₂O₂ solution (230-100%-TSCG) are 27.00 MJ∙kg⁻¹, 292 °C, and 120 kg∙m⁻³, respectively. This HHV accounts for a 29% improvement compared to that of untorrefied SCG. The contact angle (126°), water activity (0.51 aw), and moisture content (7.69%) of the optimized biochar indicate that it has higher resistance against biodegradation, and thereby can be stored longer. Overall, H₂O₂ is a green treatment additive for SCGs solid fuel. This study has successfully produced biochar with greater HHV and low bulk density at low temperatures. The green additive development can effectively reduce environmental pollutants and upgrade wastes into resources, and achieve “3E”, namely, environmental (non-polluting green additives), energy (biofuel), and circular economy (waste upgrade). In addition, the produced biochar has great potential in the fields of bioadsorbents and soil amendments.

Toluene is a highly flammable and commonly used industrial chemical with severe health consequences on humans upon exposure and ingestion. In this study, multispecies bioassays were conducted using a species sensitivity distribution approach to determine acute and chronic hazardous concentrations of toluene in soil. Acute and chronic toluene toxicity tests were conducted with seven soil species from four taxonomic groups. The results from the toxicity tests were used to estimate the acute and chronic HC₅ (hazardous concentration for 5 % of species) of toluene in the terrestrial environment at 58.9 (5.4–639.6) mg kg⁻¹ and 2.2 (0.2–19.8) mg kg⁻¹, respectively. To the best of our knowledge, this is the first study to estimate the hazardous concentration of toluene in soil by conducting a battery of bioassays. These values can be used as references for the environmental risk assessment of chemical accidents involving toluene and estimating its impact on soil to protect the terrestrial environment.

Heavy metal pollution in paddy fields has caused widespread concerns due to the threat to food safety. The present study used low-cost sugarcane bagasse (SB) and two sugarcane bagasse materials modified with citric-acid (SSB) and citric-acid/Fe₃O₄ (MSB) to investigate their effects on the bioavailability of Cd in soil and Cd accumulations in rice in a pot experiment. The three organic amendments significantly decreased the Cd accumulation in plants by limiting its mobilization in soil. The MSB and SSB but not SB increased the soil pH and immobilized the Cd in soil significantly during the 120-day experiment. The amendments decreased Cd bioavailability through transforming to the stable fraction throughout the whole growth stage. The functional groups in the amendments (-OH, –COOH, C–O, -COO⁻ and Fe–O) and precipitates [Cd(NO₂)₂K(NO₂)₂, Cd(OH)₂ and Cd₇₅Zn₂₅Fe₂O₄] played active roles in Cd immobilization. Moreover, the three organic materials increased the content of Fe–Mn plaque on rice roots, which prevented its transport from soil to rice roots further. We also found that Fe competed with Cd for transporters and reduced potential Cd uptake and translocation in rice tissues. The addition of MSB and SB but not SSB inhibited the rice growth compared to the unamended control, indicating the potential of SSB in situ remediation. These results provide valuable information to use organic amendments for Cd passivation in soil and food safety.

In the past decade, particulate matter with aerodynamic diameter less than 2.5 μm (PM₂.₅) has reached unprecedented levels in China and posed a significant threat to public health. Exploring the long-term trajectory of the PM₂.₅ attributable health burden and corresponding disparities across populations in China yields insights for policymakers regarding the effectiveness of efforts to reduce air pollution exposure. Therefore, we examine how the magnitude and equity of the PM₂.₅-related public health burden has changed nationally, and between provinces, as economic growth and pollution levels varied during 2005–2017. We derive long-term PM₂.₅ exposures in China from satellite-based observations and chemical transport models, and estimate attributable premature mortality using the Global Exposure Mortality Model (GEMM). We characterize national and interprovincial inequality in health outcomes using environmental Lorenz curves and Gini coefficients over the study period. PM₂.₅ exposure is linked to 1.8 (95% CI: 1.6, 2.0) million premature deaths over China in 2017, increasing by 31% from 2005. Approximately 70% of PM₂.₅ attributable deaths were caused by stroke and IHD (ischemic heart disease), though COPD (chronic obstructive pulmonary disease) and LRI (lower respiratory infection) disproportionately affected poorer provinces. While most economic gains and PM₂.₅-related deaths were concentrated in a few provinces, both gains and deaths became more equitably distributed across provinces over time. As a nation, however, trends toward equality were more recent and less clear cut across causes of death. The rise in premature mortality is due primarily to population growth and baseline risks of stroke and IHD. This rising health burden could be alleviated through policies to prevent pollution, exposure, and disease. More targeted programs may be warranted for poorer provinces with a disproportionate share of PM₂.₅-related premature deaths due to COPD and LRI.

It is widely recognized that green infrastructures in urban ecosystems provides important ecosystem services, including air purification. The potential absorption of nitrogen oxides (NOₓ) by urban trees has not been fully quantified, although it is important for air pollution mitigation and the well-being of urban residents. In this study, four common tree species (Sophora japonica L., Fraxinus chinensis Roxb., Populus tomentosa Carrière, Sabina chinensis (L.)) in Beijing, China, were studied. The dual stable isotopes (¹⁵N and ¹⁸O) and a Bayesian isotope mixing model were applied to estimate the sources contributions of potential nitrogen sources to the roadside trees based on leaf and soil sampling in urban regions. The following order of sources contributions was determined: soil > dry deposition > traffic-related NOₓ. The capacity of urban trees for NOₓ removal in the city was estimated using a remote sensing and GIS approach, and the removal capacity was found to range from 0.79 to 1.11 g m⁻² a⁻¹ across administrative regions, indicating that 1304 tons of NOₓ could be potentially removed by urban trees in 2019. Our finding qualified the potential NOₓ removal by urban trees in terms of atmospheric pollution mitigation, highlighting the role of green infrastructure in air purification, which should be taken into account by stakeholders to manage green infrastructure as the basis of a nature-based approach.

Porous silicon-coated zero-valent iron (Fe⁰@p-SiO₂) is a promising material for in-situ contaminated groundwater remediation. However, investigations of factors that affect the transport of Fe⁰@p-SiO₂ remain incomplete. In the present study, Fe⁰@p-SiO₂ composites were prepared by a SiO₂-coated technology, and a series of column experiments were conducted to examine the effects of media size, ionic strength, and injection velocity and concentration on retention and transport in saturated porous media. Results showed that the obtained Fe⁰@p-SiO₂ is a core-shell composite with zero-valent iron as the core and porous silicon as the shell. Media size, injection velocity, Fe⁰ concentration, and ionic strength had a significant impact on the transport of Fe⁰@p-SiO₂. Fe⁰@p-SiO₂ effluent concentrations decreased with a smaller media size. Increasing initial particle concentration and ionic strength led to a decrease in particle transport. High particle retention was observed near the middle of the column, especially with high injection concentration. That was also observable in the condition of lower injection velocity or finer media. The results indicated that two transport behaviors during particles transport, which were “agglomeration-straining” and “detachment-re-migration”. Moreover, the dominated mechanisms for Fe⁰@p-SiO₂ transport and retention in saturated porous media are hydrodynamic dispersion and interception. Given the results, in practical engineering applications, proper injection velocity and concentration should be selected depending on the pollution status of groundwater and the geochemical environment to ensure an effective in-situ reaction zone.

Atmospheric peroxyacetyl nitrate (PAN) and ozone (O₃) are two typical indicators for photochemical pollution that have adverse effects on the ecosystem and human health. Observation networks for these pollutants have been expanding in developed regions of China, such as North China Plain (NCP) and Pearl River Delta (PRD), but are sparse in Yangtze River Delta (YRD), meaning their concentration and influencing factors remain poorly understood. Here, we performed a one-year measurement of atmospheric PAN, O₃, particulate matter with aerodynamic diameter smaller than 2.5 μm (PM₂.₅), nitrogen oxides (NOₓ), carbon monoxide (CO), and meteorological parameters from December 2016 to November 2017 in Shanghai. Overall, high hourly maximum PAN and O₃ were found to be 7.0 and 185 ppbv in summer, 6.2 and 146 ppbv in autumn, 5.8 and 137 ppbv in spring, and 6.0 and 76.7 ppbv in winter, respectively. Continental air masses probably carried atmospheric pollutants to the sampling site, while frequent maritime winds brought in less polluted air masses. Furthermore, positive correlations (R: 0.72–0.85) between PAN and O₃ were found in summer, indicating a predominant role of photochemistry in their formation. Unlike in summer, weak or no correlations between PAN and O₃ were featured during the other seasons, especially in winter, due to their different loss pathways. Unexpectedly, positive correlations between PAN and PM₂.₅ were found in all seasons. During summer, moderate correlation could be attributed to the strong photochemistry acting as a common driver in the formation of secondary aerosols and PAN. During winter, high PM₂.₅ might promote PAN production through HONO production, hence resulting in a good positive correlation. Additionally, the loss of PAN by thermal decomposition (TPAN) only accounted for a small fraction (ca. 1%) of the total (PAN + TPAN) during a typical winter episode, while it significantly reached 14.4 ppbv (71.1% of the total) in summer.

Ammonium molybdophosphate (AMP) exhibits high selectivity towards Cs but it cannot be directly applied in column packing, so it is necessary to prepare AMP–based adsorbents into an available form to improve its practicality. This work provided two AMP immobilized cellulose microspheres (MCC@AMP and MCC-g-AMP) as adsorbents for Cs removal by radiation grafting technique. MCC-g-AMP was prepared by radiation graft polymerization of GMA on microcrystalline cellulose microspheres (MCC) followed by reaction with AMP suspension, and MCC@AMP was synthesized by radiation hybrid grafting of AMP and GMA onto MCC through one step. The different structures and morphologies of two adsorbents were characterized by FTIR and SEM. The adsorption properties of two adsorbents against Cs were investigated and compared in batch and column experiments under different conditions. Both adsorbents were better obeyed pseudo-second-order kinetic model and Langmuir model. MCC-g-AMP presented faster adsorption kinetic and more stable structure, whereas MCC@AMP presented more facile synthesis and higher adsorption capacity. MCC@AMP was pH independent in the range of pH 1–12 but MCC-g-AMP was sensitive to pH for Cs removal. The saturated column adsorption capacities of MCC@AMP and MCC-g-AMP were 5.4 g-Cs/L-ad and 0.75 g-Cs/L-ad in column adsorption experiments at SV 10 h⁻¹. Both adsorbents exhibited very high radiation stability and can maintain an adsorption capacity of >85% even after 1000 kGy γ-irradiation. On the basis, two AMP-loaded adsorbents possessed promising application in removal of Cs from actual contaminated groundwater. These findings provided two efficient adsorbents for treatment of Cs in radioactive waste disposal.

Particulate matter (PM) had been associated with cardiotoxicity, while the mechanism of toxicity has yet to be elucidated, with mitochondria dysfunction as a potential candidate. To investigate the potential cardiotoxic effects of ambient PM exposure and assess the damage to cardiac mitochondria, C57/B6 mice were exposed to filtered air or real ambient PM for three or six weeks. Furthermore, to reveal the role of peroxisome proliferators-activated receptor alpha (PPAR alpha) in PM exposure induced cardiotoxicity/mitochondria damage, animals were also co-treated with PPAR alpha agonist WY 14,643 or PPAR alpha antagonist GW 6471. Cardiotoxicity was assessed with echocardiography and histopathology, while mitochondria damage was evaluated with mitochondria membrane potential measurement and transmission electron microscopy. Potential impacts of PM exposure to PPAR alpha signaling were detected with co-immunoprecipitation and western blotting. The results indicated that exposure to ambient PM exposure induced cardiotoxicity in C57/B6 mice, including altered cardiac functional parameters and morphology. Cardiac mitochondria damage is detected, in the form of compromised mitochondria membrane potential and morphology. Molecular investigations revealed disruption of PPAR alpha interaction with peroxisome proliferator-activated receptor gamma coactivator-1A (PGC-1a) as well as altered expression levels of PPAR alpha downstream genes. Co-treatment with WY 14,643 alleviated the observed toxicities, while co-treatment with GW 6471 had mixed results, exaggerating most cardiotoxicity and mitochondrial damage endpoints but alleviating some cardiac functional parameters. Interestingly, WY 14,643 and GW 6471 co-treatment seemed to exhibit similar regulative effects towards PPAR alpha signaling in animals exposed to PM. In conclusion, ambient PM exposure indeed induced cardiotoxicity in C57/B6 mice, in which cardiac mitochondria damage and disrupted PPAR alpha signaling are contributors.