The synthetic polycyclic musks HHCB (Galaxolide®) and AHTN (Tonalide®) were monitored in fathead minnows (FHMs) caged for a month at various locations in the North Saskatchewan River (NSR), upstream and downstream of the Gold Bar wastewater treatment plant that serves the city of Edmonton, AB, Canada. In addition, the distribution of these musk compounds in the river was predicted using the fugacity-based Quantitative Water Air Sediment Interface (QWASI) model. In FHMs caged 0.15 km downstream of the wastewater outfall, mean concentrations of HHCB and AHTN were 7.4 and 0.4 μg g−1 wet weight, respectively. These are among the highest reported concentrations of these musk compounds in fish exposed to treated wastewater. The musk concentrations in FHMs were significantly lower further downstream of the outfall. High bioconcentration factors (BCFs) in FHMs that exceeded 104 higher than estimated concentrations in water indicated that there were low rates of biotransformation of the musks in the fish. In the FHMs caged at the site closest to the wastewater outfall, HHCB concentrations in FHMs were comparable to the body burdens that have been reported to moderate expression of vitellogenin in female rainbow trout, indicating that fish in the NSR downstream of the wastewater outfall may be at risk of anti-estrogenic effects. The QWASI model applied to six individual river sections of the NSR predicted that the largest fluxes of HHCB and AHTN would be for downstream transport in water, which explains why FHMs accumulated elevated concentrations of the musks at the furthest downstream site, 9.9 km from the wastewater discharge.

Biochar is a promising material used in soil amendment and carbon nanotubes may enter soil due to its increasing application. These carbonaceous materials may change the bioavailability of pollutants in soil. In this concern, 0.5% w/w multi-walled carbon nanotubes (MWCNTs) and 3 corn-straw biochars acquired at different pyrolyzing temperatures were used in soil amendment and their influences on the bioavailability of hexabromocyclododecanes (HBCDs), a brominated flame retardant, to 2 ecologically different earthworm species were studied. The amendment of 4 carbonaceous materials all reduced the bioaccumulation of HBCDs in earthworms by 18.2%–67.3%, which varied depending on the type of carbonaceous materials and the pyrolyzing temperature of biochars. The reduction in HBCDs uptake by Eisenia fetida (an epigeic species) was greater than by Metaphire guillelmi (an anecic species). The 2 earthworm species both showed bioaccumulative selectivity on certain HBCD diastereoisomer and enantiomer in the amended soils, which was similar to that in the control soil. Moreover, Tenax-assisted HBCDs desorption test was carried out for the simulation of their bioavailability. The rapid desorption fraction (Frap), total desorption (15 d), and 24 h desorption all correlated well with the uptake of HBCDs in the earthworms, suggesting that the 24 h-desorption, due to its easy availability, can be a good proxy to predict the bioavailability of HBCDs to earthworms in soil.

Previous studies investigating health conditions of individuals living near livestock farms generally assessed short time windows. We aimed to take time-specific differences into account and to compare the prevalence of various health conditions over seven consecutive years. The sample consisted of 156,690 individuals registered in 33 general practices in a (rural) area with a high livestock density and 101,015 patients from 23 practices in other (control) areas in the Netherlands. Prevalence of health conditions were assessed using 2007–2013 electronic health record (EHR) data. Two methods were employed to assess exposure: 1) Comparisons between the study and control areas in relation to health problems, 2) Use of individual estimates of livestock exposure (in the study area) based on Geographic Information System (GIS) data. A higher prevalence of chronic bronchitis/bronchiectasis, lower respiratory tract infections and vertiginous syndrome and lower prevalence of respiratory symptoms and emphysema/COPD was found in the study area compared with the control area. A shorter distance to the nearest farm was associated with a lower prevalence of upper respiratory tract infections, respiratory symptoms, asthma, COPD/emphysema, allergic rhinitis, depression, eczema, vertiginous syndrome, dizziness and gastrointestinal infections. Especially exposure to cattle was associated with less health conditions. Living within 500m of mink farms was associated with increased chronic enteritis/ulcerative colitis. Livestock-related exposures did not seem to be an environmental risk factor for the occurrence of health conditions. Nevertheless, lower respiratory tract infections, chronic bronchitis and vertiginous syndrome were more common in the area with a high livestock density. The association between exposure to minks and chronic enteritis/ulcerative colitis remains to be elucidated.

Pharmaceuticals are increasingly detected in environmental matrices, but information on their trophic transfer in aquatic food webs is insufficient. This study investigated the bioaccumulation and trophic transfer of 23 pharmaceuticals in Taihu Lake, China. Pharmaceutical concentrations were analyzed in surface water, sediments and 14 aquatic species, including plankton, invertebrates and fish collected from the lake. The median concentrations of the detected pharmaceuticals ranged from not detected (ND) to 49 ng/L in water, ND to 49 ng/g dry weight (dw) in sediments, and from ND to 130 ng/g dw in biota. Higher concentrations of pharmaceuticals were found in zoobenthos relative to plankton, shrimp and fish muscle. In fish tissues, the observed pharmaceutical contents in the liver and brain were generally higher than those in the gills and muscle. Both bioaccumulation factors (median BAFs: 19–2008 L/kg) and biota−sediment accumulation factors (median BSAFs: 0.0010–0.037) indicated a low bioaccumulation potential for the target pharmaceuticals. For eight of the most frequently detected pharmaceuticals in food webs, the trophic magnification factors (TMFs) were analyzed from two different regions of Taihu Lake. The TMFs for roxithromycin, propranolol, diclofenac, ibuprofen, ofloxacin, norfloxacin, ciprofloxacin and tetracycline in the two food webs ranged from 0.28 to 1.25, suggesting that none of these pharmaceuticals experienced trophic magnification. In addition, the pharmaceutical TMFs did not differ significantly between the two regions in Taihu Lake.

Global warming and chemical pollution are key anthropogenic stressors with the potential to interact. While warming can change the impact of pollutants and pollutants can change the sensitivity to warming, both interaction pathways have never been integrated in a single experiment. Therefore, we tested the effects of warming and multiple pesticide pulses (allowing accumulation) of chlorpyrifos on upper thermal tolerance (CTmax) and associated physiological traits related to aerobic/anaerobic energy production in the damselfly Ischnura elegans. To also assess the role of latitude-specific thermal adaptation in shaping the impact of warming and pesticide exposure on thermal tolerance, we exposed larvae from replicated high- and low-latitude populations to the pesticide in a common garden rearing experiment at 20 and 24 °C, the mean summer water temperatures at high and low latitudes. As expected, exposure to chlorpyrifos resulted in a lower CTmax. Yet, this pesticide effect on CTmax was lower at 24 °C compared to 20 °C because of a lower accumulation of chlorpyrifos in the medium at 24 °C. The effects on CTmax could partly be explained by reduction of the aerobic scope. Given that these effects did not differ between latitudes, gradual thermal evolution is not expected to counteract the negative effect of the pesticide on thermal tolerance. By for the first time integrating both interaction pathways we were not only able to provide support for both of them, but more importantly demonstrate that they can directly affect each other. Indeed, the warming-induced reduction in pesticide impact generated a lower pesticide-induced climate change sensitivity (in terms of decreased upper thermal tolerance). Our results indicate that, assuming no increase in pesticide input, global warming might reduce the negative effect of multiple pulse exposures to pesticides on sensitivity to elevated temperatures.

The specific area between biochar and soil surfaces is termed the “charosphere”, which is analogous to the rhizosphere between plant roots and soil. Physicochemical properties of charosphere soil differ from natural soil. A double-layer mesh bag experiment was conducted to separate the charosphere soil (>2 mm) from biochar (derived from manure pyrolyzed at 300, 500, 700 °C) and the bulk soil. A 95-day experiment determined the availability, total concentration and speciation of Cd in charosphere soil and the total and available Cd in biochar. The availability of Cd decreased in the charosphere soils. Total Cd concentration increased in the biochars but decreased in charosphere soil, suggesting that Cd might transfer from charosphere soil to biochar. Cd in the acid soluble fraction decreased by 25–40% in different biochars while it increased in residue fraction, indicating that Cd tends to be more stable in charosphere soil. Instrumental analysis using SEM, FTIR were performed to provide further evidence of the transport of Cd and to enable interpretation of the mechanisms involved. The phenomena that Cd concentrations change markedly in the charosphere inspires a novel research perspective towards the study of transport and transformation of heavy metals in soil after biochar application.

In the study, the capability of Paecilomyces lilacinus XLA (CCTCC: M2012135) to reduce Cr6+ and its main antagonistic mechanisms to Cr6+ were experimentally evaluated. Activated growing fungus XLA efficiently reduced over 90% Cr6+ in the media with Cr6+ concentration below 100 mg L−1 at pH 6 after 14 days. After 1-day exposure to 100 mg L−1 Cr6+, nearly 50% of Cr6+ was reduced. Moreover, SO42− stimulated Cr6+ reduction, whereas other interferential ions inhibited Cr6+ reduction. The interaction mechanisms between XLA and Cr6+ mainly involve biotransformation, biosorption, and bioaccumulation, as detected by electron microscopy and chemical methods. The lower concentrations of Cr6+ (5 and 50 mg L−1) stimulated the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) level in XLA, respectively, but the higher concentration of Cr6+ (150 mg L−1) decreased the enzymatic activities and GSH concentration. The results implied that SOD, CAT and GSH were defensive guards to the oxidant stress produced by Cr6+. All these extracellular/intracellular defense systems endowed XLA with the ability to resist and detoxify Cr6+ by transforming its valent species. The fungus XLA could efficiently reduce Cr6+ under different environmental conditions (pH, interferential ions, and concentration). Moreover, XLA could endure the high concentration of Cr6+ probably due to its high biotransformation capability of Cr6+ and intracellular antioxidant systems for the detoxification of ROS generated by external Cr6+. All these results suggested that the fungus XLA can be applied to remediation of Cr6+-contaminated environments.

This study sought to evaluate the toxicological effects of graphene oxide (GO) through tests with Danio rerio (zebrafish) embryos, considering the influence of the base washing treatment and the interaction with natural organic matter (i.e., humic acid, HA). A commercial sample of GO was refluxed with NaOH to remove oxidation debris (OD) byproducts, which resulted in a base washed GO sample (bw-GO). This process decreased the total oxygenated groups in bw-GO and its stability in water compared to GO. When tested in the presence of HA, both GO and bw-GO stabilities were enhanced in water. Although the embryo exposure showed no acute toxicity or malformation, the larvae exposed to GO showed a reduction in their overall length and acetylcholinesterase activity. In the presence of HA, GO also inhibited acid phosphatase activity. Our findings indicate a mitigation of material toxicity after OD removal. The difference in the biological effects may be related to the materials’ bioavailability and biophysicochemical interactions. This study reports for the first time the critical influence of OD on the GO material biological reactivity and HA interaction, providing new data for nanomaterial environmental risk assessment and sustainable nanotechnology.

Biochar-supported nanoscale zero-valent iron (nZVI) produced under different temperatures was studied to evaluate the effect of the nZVI-biochar composite on the removal of hexavalent chromium (Cr(VI)) in solution. The structure of biochar-supported nZVI and its roles in Cr(VI) removal were investigated by X-Ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and batch experiments. The XRD revealed that the removal rate of Cr(VI) for the nZVI supported by rice straw pyrolyzed at 400 °C (RS400) was much greater than that for other supporting biochar, and the FTIR further indicated that the carboxyl groups and silicon mineral within the biochar served as dual support sites for nZVI. NZVI-RS400 exhibited the highest removal amount of Cr(VI) at approximately 40.0 mg/g under an initial pH of 4.0, possibly due to both the reduction and adsorption processes. Therefore, the RS400-supported nanoscale zero-valent iron could be a preferable material for Cr(VI)-contaminated groundwater.

A systematic grid sampling method and geostatistics were employed to investigate the spatial distribution, inventory, and potential ecological and human health risks of the residues of hexachlorobenzene (HCB) and chlorinated cyclodiene pesticides in soils of the Campanian Plain, Italy, and explore their relationship with the soils properties. The geometric mean (Gmean) concentrations of HCB and cyclodiene compounds followed the order CHLs (heptachlor, heptachlor epoxide, trans-chlordane, and cis-chlordane) > DRINs (aldrin, dieldrin, and endrin) > SULPHs (α-endosulfan, β-endosulfan, and endosulfan sulfate) > HCB. The residual levels of most cyclodienes in agricultural soils were generally higher than those of corresponding counterparts in the other land uses. Significant differences in the concentration of HCB and cyclodienes in the soils across the region are observed, and the Acerra-Marigliano conurbation (AMC) and Sarno River Basin (SRB) areas exhibit particularly high residual concentrations. Some legacy cyclodienes in the Campanian Plain may be attributed to a secondary distribution. The Gmean inventory of HCB, SULPHs, CHLs, and DRINs in the soil is estimated to be 0.081, 0.41, 0.36, and 0.41 metric tons, respectively. The non-cancer and cancer risks of HCB and cyclodienes for exposed populations are deemed essentially negligible, however, endosulfan poses significant ecological risks to some terrestrial species.