This study mainly focuses on welfare impacts from improving considerably degraded river stretches of Heihe river basin and to determine the impact of spatial heterogeneity on willingness-to-pay (WTP) for integrated river basin management (IRBM). The significant WTP values for ecological attributes improvement revealed that the respondents of Heihe river basin significantly support the integrated ecological restoration program. However, socioeconomic characteristics and residential location have a significant impact on WTP values for different ecological attributes. The results demonstrate that water quality is the most preferred variable among all the river attributes in the study area. For example, in Zhangye, Gaotai, Ejinaqi, Minle, and Sunan the respondents were willing to pay 90.6, 160.44, 377.15, 65.40, and 139.21 Yuan per year, respectively, for a one-grade improvement in current water quality. Our findings also show that all the major counties of the basin concern more about water quality representing the relative importance of river water and express the maximum WTP for its improvement, while all major counties also share a low WTP for improvements in conditions of the leisure and entertainment. The differences in socioeconomic characteristics and ecological status of the respondents partially explain the disparity in utility from IRBM. In conclusion, the results based on only specific sub-basin of river basin may over or underestimate the welfare estimate.

To investigate the characteristics and formation mechanisms of haze pollution in the autumn season in the Sichuan Basin, hourly concentrations of water-soluble inorganic ions in PM₂.₅ (Na⁺, K ⁺, NH₄⁺, Mg²⁺, Ca²⁺, Cl⁻, NO₃⁻, and SO₄²⁻) and major gaseous precursors (HCl, NH₃, SO₂, HONO, and HNO₃) were measured by a gas and aerosol collector combined with ion chromatography (GAC-IC) from September to November 2017 at an urban site in Chengdu. The average mass concentration of total water-soluble ions was 36.9 ± 29.4 μg m⁻³, accounting for 62.8% of PM₂.₅ mass. Nitrate was the most abundant ion, comprising 41.2% of the total ions, followed by sulfate (27.1%) and ammonium (18.1%), indicating the important contribution of motor vehicle emissions to PM₂.₅ in Chengdu. Secondary formation of inorganic ions and biomass burning emissions played a vital role in the haze pollution processes. The formation of nitrate aerosol was particularly dominant and exhibited the most substantial increase during haze processes. It was likely to be produced primarily through homogeneous reactions, whereas heterogeneous reactions dominated sulfate formation. Additionally, distinct differences in diurnal patterns of secondary inorganic ions between clean days and polluted days were observed, reflecting different formation characteristics under polluted conditions. Due to a large increase of acidic aerosols, most particles collected on polluted days were acidic, and ammonium in most samples existed mainly as NH₄HSO₄ and NH₄NO₃. Furthermore, backward-trajectory cluster analysis revealed that air masses originating from the northeast of Chengdu prevailed in the autumn season, and haze pollution was dominated mainly by short-distance transport within the Sichuan Basin.

Microplastics (MPs) in natural environments have attracted lots of attention. Although the quantity of MPs present in terrene is much higher than that in aquatic environment, few studies have investigated the chemical behavior of MPs in terrestrial environment. This study investigate the Cu²⁺ (as a model heavy metal) adsorption capacity of six kinds of MPs (polyamide-6 (PA), polyethylene (PE), polystyrene (PS), polyethylene terephthalate (PET), polyvinyl chloride (PVC), polymethyl methacrylate (PMMA)) in batch adsorption experiments and the effects of different soil environmental factors, including pH and the presence of cations and low-molecular-weight organic acids (LMWOAs), as well as ultraviolet (UV) aging. The Cu²⁺ adsorption capacities of PA and PMMA were higher than those of other MPs and their maximum equilibrium adsorption capacities (estimated by the Langmuir adsorption equation) were 323.6 μg/g ± 38.2 and 41.03 ± 1.78 μg/g, respectively. The Cu²⁺ adsorption on MPs was affected by pH, and the greatest amount of Cu²⁺ adsorbed on PA and PMMA was observed at pH = 6 and pH = 7, respectively. The presence of Ca²⁺ or Mg²⁺ inhibited Cu²⁺ adsorption by MPs, due to competition for the adsorption sites. Moreover, Cu²⁺ adsorption by MPs was affected by various types of LMWOAs. The Cu²⁺ adsorption on PA was significantly reduced by citric acid, followed by oxalic acid, and oxalic acid was particularly evident for Cu²⁺ adsorption on PMMA. UV aging (200 h) had different effect on Cu²⁺ adsorption on MPs and it depends on the change of carbonyl index. Results demonstrate that soil environmental factors can change the ability of different MPs to adsorb Cu²⁺ and affect the transport of pollutants as carriers.

Although coastal marshes are net carbon sinks, they are net methane sources. Aerobic methanotrophs in coastal marsh soils are important methane consumers, but their activity and populations are poorly characterized. DNA stable-isotope probing followed by sequencing was used to determine how active methanotrophic populations differed in the main habitats of a Chinese coastal marsh. These habitats included mudflat, native plant-dominated, and alien plant-dominated habitats. Methylococcaceae was the most active methanotroph family across four habitats. Abundant methylotroph sequences, including methanotrophs and non-methane-oxidizing methylotrophs (Methylotenera and Methylophaga), constituted 50–70% of the 16S rRNA genes detected in the labeled native plant-dominated and mudflat soils. Methylotrophs were less abundant (~ 20%) in labeled alien plant-dominated soil, suggesting less methane assimilation into the target community or a different extent of carbon cross-feeding. Canonical correspondence analysis indicated a significant correlation between the active bacterial communities and soil properties (salinity, organic carbon, total nitrogen, pH, and available phosphorus). Importantly, these results highlight how changing vegetation or soil features in coastal marshes may change their resident active methanotrophic populations, which will further influence methane cycling.

Arsenic exposure by groundwater contamination is a menace which threatens more than 26 million individuals of West Bengal. Interestingly, with similar levels of arsenic exposure, only 15–20% of the population show arsenic-induced skin lesions, the hallmarks of chronic arsenic toxicity, but the rest do not. In this study, our aim was to identify whether microRNAs (miRNA) have any role to play in causing such arsenic susceptibility. Global plasma miRNA profiling was done in 12 arsenic-exposed individuals with skin lesions and 12 exposed individuals without skin lesions. Two hundred two miRNAs were found to be differentially regulated between the two study groups. Results were validated by quantitative real-time PCR in 30 exposed subjects from each of the groups, which showed that among others miR-21, miR-23a, miR-27a, miR-122, miR-124, miR-126, miR-619, and miR-3613 were significantly upregulated and miR-1282 and miR-4530 were downregulated in the skin lesion group compared with the no skin lesion group. Bioinformatic analyses predicted that these altered miRNAs have targets in 7 different biochemical pathways, including glycerophospholipid metabolism, colorectal cancer, glycosphingolipid biosynthesis, T cell receptor signaling, and neurotrophin signaling pathways; glycerophospholipid metabolism pathway being the most enriched pathway. Association study show that these microRNAs contribute significantly to the increased prevalence of other non-dermatological health effects like conjunctival irritations of the eyes and respiratory distress in the study subjects. To our knowledge, this is the first study of its kind involving miRNA expressions contributing to arsenic susceptibility in the exposed population of West Bengal.

Composting is an attractive way to recycle organic wastes because the product (compost) can be used as an organic fertilizer or a culture substrate. This study assessed coal fly ash (CFA; at 0, 15, and 35%) and/or vinegar residue (VR; at 0, 35, and 55%) as additives in the green waste (GW) composting process. Compost maturity was assessed based on the following indicators: water-holding capacity, pH, total organic carbon, electrical conductivity, total Kjeldahl nitrogen, and germination index. Other important compost properties that were determined included germination percentage and root length of seeds in a germination assay, crude fiber degradation, specific UV absorption, E₄/E₆ ratio, microbial numbers (culturable bacteria and volatile fatty acid-degrading bacteria), enzyme activities (dehydrogenase, β-glucosidase, acid-phosphatase, urease, and ortho-diphenol oxidase), and available nutrients. When added together, CFA and VR improved all of these properties. As indicated by the maturity indicators and other properties, the best treatment (the combined addition of 15% CFA and 55% VR) required only 23 days to produce a mature and high quality compost.

In soil, vanadium (V) contamination is commonly concomitant with chromium (Cr) contamination, which poses potential risks to humans, animals, and plants due to the transfer of toxic metals and the increase in their concentrations via the food chain or through direct exposure. This study applied a multi-step column leaching process using low-molecular-weight organic acids (LMWOAs) to treat V-contaminated soil from a smelter site that contains 2015.1 mg V kg⁻¹ and 1060.3 mg Cr kg⁻¹. After leaching three times with an equivalent solution/soil ratio of 0.3 mL/g using 1.0 M oxalic acid solution, the total removal rates reached 77.2% and 7.2% for V and Cr, respectively, while the removal rates of the extractable fractions reached 118.6% and 99.2% due to the reduction in residual fraction (F₄) of toxic metals. Simultaneously, the distribution and redistribution of geochemical fractions of V and Cr were determined with a sequential extraction technique, and the greater proportion of potential mobile fractions of V (65.1%) may increase its leaching from soil relative to Cr (7.1%). In addition, a lower pH of the leaching agent increased the efficiency of the leaching process to an extent. Compared with batch extraction with a typical solution to soil ratio of 10 mL/g, multi-step column leaching used less agent and hence produced less wastewater. This strategy could reduce the mobilization and bioavailability of toxic metals, and potentially enhance in situ soil flushing for the remediation of V- and Cr- contaminated soil.

Maternal exposure to high levels of persistent organic pollutants (POPs) and trace elements is an important concern for fetal growth. In our previous study, we showed the polychlorinated biphenyl (PCB) levels in maternal serum from the Chiba Study of Mother and Child Health (C-MACH) cohort and their relationships between PCB levels in cord serum with birth weight of newborn. Various reports on the relationship between chemical exposure and birth status have been published; however, studies that analyze the effects of both PCB and metal exposure together in one cohort are still limited. In this study, we aimed to determine the relationship of maternal serum levels of PCBs and toxic and essential trace elements [mercury (Hg), manganese (Mn), selenium (Se), and cadmium (Cd)], with birth weight and head circumference, in the C-MACH cohort. The median concentration of total PCBs in maternal serum around 32 gestational weeks (n = 62) was 360 pg g⁻¹ wet wt (41 ng g⁻¹ lipid wt). The levels of Hg, Mn, Se, and Cd in maternal serum were 0.89, 0.84, 100, and 0.024 ng g⁻¹, respectively. In this study, the Bayesian linear model determined the relationships of the birth weight and head circumference with combinations of PCB levels, toxic and essential trace elements, and questionnaire data. We found that PCB concentrations in maternal serum were weakly and negatively related to birth weight, whereas trace elements were not associated with birth weight. Serum PCB and Mn levels were negatively associated with head circumference, whereas other trace elements were not associated with head circumference. These results showed that maternal exposure to PCBs may be related to birth weight and head circumference, while maternal exposure to Mn is related to head circumference, even when adjusted based on the exposure levels of other contaminants, and maternal and fetal characteristics. Therefore, our findings indicate that maternal exposure to PCBs and Mn might be negatively related with birth weight and head circumference.

Increased use of colistin, a last resort drug due to failure of carbapenems, has possibly contributed in development and spread of resistance to colistin among Enterobacteriaceae. The colistin belongs to the family of polymyxins, cationic polypeptides, with broad-spectrum activity against Gram-negative bacteria. In this study, we obtained 253 non-duplicate bacterial isolates from sewage water in Delhi and phenotypically screened for colistin resistance. Of the 47 positive isolates, the colistin resistance gene mcr-1 was detected among 5 isolates. Based on 16S ribosomal RNA–based identification, bacterial isolates were found to be Escherichia coli, Aeromonas veronii, and Aeromonas dhakensis. Extended spectrum β-lactamases (ESBL)–resistant determinants CTX-M and TEM were detected in all five mcr-1 positive isolates. On the basis of literature survey, this is the first report of mcr-1 gene from Aeromonas veronii and Aeromonas dhakensis worldwide. Furthermore, mcr-1 gene has not been reported earlier from sewage water in India. Antibiotic susceptibility test of all five isolates against 9 different classes of drugs revealed multidrug-resistant phenotype with high minimum inhibitory concentration values. In vitro transconjugation studies showed successful transfer of mcr-1 and other ESBL-resistant determinants. The occurrence of colistin resistance phenotype conferred by plasmid-based mcr-1 gene in the environment and an ever-increasing list of bacterial isolates is a cause of concern. A comprehensive survey of different water bodies and epidemiological studies are required to assess the risk of dissemination of resistance determinants.

In this paper, a flexible and efficient nano-reinforced polymer inclusion membrane (PIM) was fabricated and used for cyanide (CN⁻) extraction from water samples. Aliquat 336 (a liquid anion exchanger) was embedded in poly(vinyl chloride) (PVC) support as the extractant. Mg-Al-CO₃ layered double hydroxide (LDH) with high surface area and anion exchange ability was applied to promote the extraction efficiency of PIM. A PIM comprising 56% PVC, 40% Aliquat 336, and 4% Mg-Al-CO₃ LDH showed the best extraction efficiency. A single beam ultraviolet-visible spectrophotometer was used for the detection of cyanide. Surface morphology of the PIM was studied by field emission scanning electron microscopy. The experimental parameters influencing the extraction process were investigated and optimized. The intra- and inter-day relative standard deviations at two different concentrations were in the range of 2.8–7.6%. The dynamic range of the method was in the range of 5–500 μg L⁻¹, and the detection limit was 1.4 μg L⁻¹. The LDH reinforced PIM showed proper characteristics for the extraction of cyanide from real water and wastewater samples with recoveries between 82 and 115%.