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Influence of Yeast Bioinoculant Isolated from Indian Date Palm Tree (Phoenix sylvestris) Sap on the Health of Wheat Crop and Soil
2023
M. S. Ambawade, N. V. Manghwani, P. R. Madhyani, A. M. Shaikh, D. D. Patil and G. R. Pathade
In this study, three promising yeast isolates were isolated from the sap of the Indian date palm tree (Phoenix sylvestris) and characterized by biochemical tests and 18S rRNA gene sequencing. They were confirmed as Saccharomyces cerevisiae and were designated as strains PYS-1, PYS-2, and PYS-3. These confirmed strains were used for the preparation of bioinoculants. Bioinoculant was prepared and applied to wheat crops, and the effect of Bioinoculant. Statistical analysis is carried out using analysis of variance (ANOVA), and it is found that the absorbance of chlorophyll, protein, and Indole Acetic Acid (IAA) content is significantly increased. The treatment of bioinoculant showed that crops significantly increased chlorophyll, protein, and IAA content. Further, we applied bioinoculant on the soil and measured the soil’s humus content before and after the treatment of bioinoculant. Then, a paired t-test was applied to check the effectiveness of the treatment, and it was found to significantly increase humus content in the soil. The use of bioinoculants is an economically feasible and eco-friendly method.
اظهر المزيد [+] اقل [-]Effect of Soil and Foliar Application of Humic Acid and Brassinolide on Morpho-physiological and Yield Parameters of Black Gram (Vigna mungo)
2023
More Kiran Narayan, Anita Jaswal and Arshdeep Singh
During the Kharif season of 2022-2023 at Lovely Professional University, Jalandhar, Punjab, a field experiment was conducted to investigate the “Effect of soil and foliar application of humic acid and brassinolide on morpho-physiological and yield parameters of Black gram (Vigna mungo).” The experiment was designed using a Randomised Block Design (RBD) with three replications and eight treatments. Compared to the other treatments, RDF + humic acid 0.1% + brassinolide 0.1ppm (foliar application) was the optimal treatment for most morphological and yield parameters. Plant height (cm), number of primary branches per plant, number of secondary branches per plant, dry matter accumulation (g), chlorophyll Index (SPAD), and leaf area (cm2) were highest under T7- RDF + humic acid 0.1%+brassinolide 0.1ppm (foliar applied) conditions. Minimum phenological observations were recorded for soil and foliar applications of brassinolide, including days to first flowering, days to 50 percent flowering, and days to pod initiation. Number of pods /plant, pod length(cm), pod weight (g), no. of seeds /pod, test weight (g), seed yield (q/ha), stover yield (q/ha), and harvest index (%) were significantly influenced by the T7 and recorded higher values. The increased seed yield may be attributable to plants treated with growth regulators remaining physiologically more active to accumulate sufficient food reserves for developing blossoms and seeds.
اظهر المزيد [+] اقل [-]Ambient Air Quality Monitoring with Reference to Particulate Matter (PM10) in Kolhapur City
2023
C. S. Bhosale, P. R. Mane, J. S. Salunkhe, V. M. Mothgare, S. S. Sutar, S. B. Manglekar, A. S. Jadhav and P. D. Raut
Air is an important medium for all living beings and is essential for the well-being of all. Monitoring of air is important to know the quality of air. The air quality monitoring was carried out in Kolhapur City under the National Air Monitoring Program. The present study involves the assessment of PM10 as described in the National Ambient Air Quality Standards (NAAQS). The source apportionment study related to particulate matter was carried out in Kolhapur City. The study also determined the average PM10 concentration in the city as it will be useful for preparing an action plan to reduce PM10 concentration. PM10 concentration was calculated as per the standard method adopted by CPCB. Sampling was carried out for 8 hours in three shifts twice a week at each sampling site for three consecutive years. Mahadwar Road (MR) and Dabholkar Corner (DC) were selected per the surrounding residential area, population density, and traffic conjunction. The third site Shivaji University (SUK), was selected as a control site. The results indicated that the PM10 level has risen above the prescribed standards of NAAQS. The reason for the rise in PM10 may be due to fossil fuel burning, construction activity, vehicles, and unpaved roads. The Analysis of Variance (ANOVA) technique is used to check the equality of the mean concentration of PM10 at these three locations and found a significant difference between mean concentrations of PM10, suggesting increased particulate matter.
اظهر المزيد [+] اقل [-]Biodiesel from Dunaliella salina Microalgae Using Base Catalyzed Transesterification – An Assessment through GC/MS, FTIR and NMR Studies
2023
V. Hariram, M. Janarthanan, R. Christu Paul, A. Sivasankar, M. Wasim Akram, E. Sangeethkumar, V. Ramanathan, P. Sajid Khan and S. Manikanta Reddy
Algal biofuels are a promising renewable feedstock to produce energy that can supplement future energy demands greatly. The present study aims to utilize Dunaliella salina, a hypersaline, unicellular greenish-orange micro-algae, to produce bio-oil. F/2 nutrient media and trace metal and vitamin solution under carbon-dioxide-rich conditions were used to cultivate the microalgae. Ultrasonic extraction method at 60 Hz for 90 min isolated 650 mL of bio-oil. A single-stage based-catalyzed transesterification process with methanol and sodium hydroxide yielded 380 mL of Pure Dunaliella salina biodiesel at % an extraction efficiency of 87%. The Phytochemical screening on the cultivated Dunaliella sp. was performed to understand its feasibility to be used as a fuel for IC engines. Furthermore, the obtained biodiesel was characterized using Fourier Transform Infrared Spectrometer (FTIR), Gas Chromatography Mass Spectrometer (GCMS), and Nuclear Magnetic Resonance (NMR) spectral analysis.
اظهر المزيد [+] اقل [-]Effects of Natural Radioactivity of Some Chemical and Organic Fertilizers on Gonads in Iraqi Kufa Markets
2023
Abdulhussein A. Alkufi, Shaymaa A. Kadhim, Azhar S. Alaboodi and Shatha F. Alhous
This study assessed the hazard indicators of common chemical and organic fertilizers widely available in the markets of Najaf Governorate, Iraq. The concentrations of natural radionuclides were measured in thirteen types of fertilizers by Gamma spectrum using NaI(Tl) (3*3) detector. The average radioactivity of the nuclides 226Ra, 232Th, and 40K was (48.91, 37.04, and 702.4675) Bq.kg-1, respectively, for (Di-Aluminum Phosphate) the chemical fertilizers of the type (DAP) and the (nitrogen, phosphorus, and potassium) chemical fertilizers of the type (NPK) the average radioactivity of the nuclides 226Ra, 232Th, and 40K were (35.78, 42.356 and 1519.653) Bq.kg-1, respectively, while the average radioactivity of the organic fertilizers (Orga.) were 55.153, 23.148 and 1451.258 for the three studied nuclei. As for the average values for radium equivalent were 155.967, 213.363, and 200.0023 (Bq.kg-1) for (DAP), (NPK) and organic fertilizers, respectively. The values of the external severity index (Hex), gamma radiation hazard index (Iγ), and representative alpha index (Iα) were within the permissible limits determined by the UNCEAR 2000. The highest value of total annual effective dose equivalent(TAED) was 1.468 mSv.y-1, the lowest value was 0.302 mSv.y-1, and the mean values were 0.722 mSv.y-1. In contrast, the highest value for annual gonadal dose equivalent(AGED) was 1392.527 μSv.y-1, the lowest value was 275.361 μSv.y-1, and the average values for all models were 672.135 μSv.y-1. The Excess Lifetime Cancer Risk (ELCR), the highest value was 4.039 × 10-3, the lowest value was 0.833× 10-3, and the average value was 1.988× 10-3 for all fertilizers. The Pearson correlation between radioactive variables and cluster analysis was recognized for the three types of fertilizer samples despite it not being widely accepted. The study can be considered preliminary data for subsequent studies.
اظهر المزيد [+] اقل [-]Economic Impact of Climate Change on Agriculture: A Study of India and its Neighbouring Countries Using ARDL Approach
2023
Mashud Ahmed and Paramita Saha
This study aims to analyze the association between the share of agriculture in GDP and changes in climatic variables, notably per capita CO2 emissions and temperature change, using time series data of India, Bangladesh, and Nepal for the period 1961-2018. The ARDL bounds testing method was applied to analyze the relationships among the research variables for both short-term and long-term. The results revealed that in the long run, per capita CO2 emissions and temperature change have no statistically significant relationship with India and Nepal’s share of agriculture in GDP. However, temperature change has demonstrated a positive and statistically significant relationship with the share of agriculture in Bangladesh’s GDP. Temperature change has a significant and adverse impact on the share of agriculture in India’s GDP in the short run, whereas CO2 has no significant effect. In the short run, CO2 shows a positive and significant connection with the share of agriculture in Bangladesh’s GDP. Still, temperature change is negatively and significantly associated with the proportion of agriculture in the nation’s GDP. Different lag values of both CO2 and temperature change have significant relationships with the share of GDP in agriculture in the short run in Nepal. As agriculture is a key source of GDP for all three countries, it is vital to implement suitable policies and make plans and strategies to mitigate climate change’s harmful consequences in agriculture.
اظهر المزيد [+] اقل [-]Risk Perception of Healthcare Workers Regarding Polymer Medical Waste Management
2023
Jasneet Kaur, Sheela Upendra and Shital Barde
Despite the relatively small amounts produced, medical waste is a significant issue for the government and the healthcare industry primarily because it poses threats to persons and the environment. Healthcare wastes pose threats to the environment and public health, but knowledge of these concerns and how they relate to management techniques is still lacking. Evaluating waste management practices and healthcare staff expertise is critical to identify shortcomings and lower risk levels. The study was conducted at three selected private hospitals. These three hospitals contain 462 beds, all together with 184 healthcare workers. The study was designed as a descriptive cross-sectional study, and three private healthcare institutions in the study region were evaluated. These 100 healthcare workers were chosen by purposive sampling. The hospitals under consideration include general surgical, Gynecological, pediatric, and various specialty treatments. Data was gathered for the study utilizing a quantitative questionnaire, which includes closed-ended structured questionnaires. The results showed that most healthcare workers practice waste segregation at source. Chemical cross-linking was very poor. However, waste shredding technology is being used substantially. Risk perception among Healthcare workers regarding polymer help waste management showed more of average to high risk. The study concluded that despite the existence of policies and plans, execution is still subpar at private healthcare institutions. The creation of guidelines for hospital waste management, their distribution to other institutions, and their encouragement to do a critical self-evaluation should all be required by the government.
اظهر المزيد [+] اقل [-]Palladium-Based Catalytic Treatment and a Rhizobacterial-Assisted Detoxification for the Enhanced Removal of Lindane
2023
Rachana Sharma and Prabhu Thangadurai
This study aimed to assess the efficacy of a bimetallic system consisting of Mg0-Pd0 and the bacterium Acinetobacter sp. for the complete detoxification of lindane. Our results demonstrate that palladium immobilized on activated charcoal achieved a removal rate of >99% for 100 mg.L-1 of Lindane within 10 minutes, with the accumulation of trace amounts of intermediates. The reductive transformation of lindane followed 1st-order kinetics, with a calculated rate constant (kobs) of 0.77 min-1. The bimetallic system resulted in the formation of a non-toxic hydrocarbon as the end-product, indicating complete dehalogenation of lindane. Furthermore, Acinetobacter sp. effectively mineralized >98% of 100 mg.L-1 of Lindane after 26 h of cultivation without any accumulation of toxic metabolite(s) in the reaction medium, demonstrating the efficiency of the biological system. Integrating both chemical and biological systems could provide significant advantages for the treatment of lindane, reducing the treatment time and overall cost. This synergistic approach can significantly enhance the overall removal efficiency of lindane from contaminated soil and water.
اظهر المزيد [+] اقل [-]Saccharification of Various Wastepaper Materials by Cellulase from Brown Garden Snail (Cornu aspersum) at Different Incubation pH Values
2023
T. M. Ndlovu and J. P. H. van Wyk
Increased solid waste pollution and the negative effect of fossil fuel consumption on the environment are issues that would require more scientific attention and application to deal effectively with these phenomena. Wastepaper, a major component of solid waste, is classified as organic waste due to the presence of cellulose, a glucose-based biopolymer that is part of its structural composition. The saccharification of cellulose into glucose, a fermentable sugar, can be achieved with a hydrolytic enzyme known as cellulase. Although cellulase from fungal species such as Trichoderma, Aspergillus, and Penicillium are well described, knowledge about cellulase isolated from the brown garden snail is limited as it has not been the subject of many research endeavors. The waste paper has been described as a suitable resource for bio-energy development due to cellulose, a structural component of this bio-material that can be degraded into glucose, a fermentable sugar. Although paper materials such as newspaper, office paper, filter paper, Woolworths and Pick and Pay (retailers) advertising paper, as well as foolscap paper, were saccharified by different cellulases, the degradation of these paper materials by garden snail cellulase is a novel investigation from our laboratory. With the effects of temperature and incubation time on this cellulase action when degraded paper materials have already been investigated and reported, this study dealt with the garden snail cellulase action when degraded paper materials at different pH values. Most of the paper materials were degraded optimally at a pH value of 6.0, while optimum saccharification was observed at pH 4.5 when newspaper and brown envelope paper were degraded, with office paper showing maximum bioconversion at pH 7.0. The difference in the structural composition of the paper materials also affects the degree of saccharification, as the amount of sugar released from the various paper materials at optimum pH values is not similar. Together with other catalytic parameters, the pH value of this enzymatic catalysis is also to be considered when designing the development of waste paper as a bio-product resource, with limiting environmental pollution as an additional advantage of this process.
اظهر المزيد [+] اقل [-]Predictability Augmentation by In-silico Study to In-vivo and In-vitro Results of Lung Doses of Airborne Fine and Ultrafine Particles Inhaled by Humans at Industrial Workplaces
2023
M. Ali
This study correlates computational predictions with in vivo and in vitro experimental results of inhaled fine and ultrafine particulate matter (PM) transport, dissemination, and deposition in the human respiratory airways. Epidemiological studies suggest that workplace exposure to anthropogenic pollutant PMs is a risk factor for increased susceptibility to acute broncho-pulmonary illnesses. However, investigations on detailed human inhalation and PM transport processes are restrictive from time, cost, and ethical perspectives. Computational simulation based on the Multiple Path Particle Dosimetry (MPPD) model was employed to quantify the risks associated with workplace exposure of these PMs. Here, the physical, mechanical, and electrical properties of PMs of carbon black (CB) and ultrafine particles (UFPs) from wire-cut electrical discharge machining (WEDM), with mass median aerodynamic diameter (CMAD) in the range of 1 nm to 1000 nm, were used as input parameters of MPPD. Additionally, it mimicked occupational workers’ age, body mass index, and oronasal-combinational nose and mouth breathing exposure time. The deposition results were compared with several vivo and in vitro experimental data reported in the literature, and satisfactory agreements were found. For example, a total lung dose of CB-PMs of 100 nm is the highest (28%), while a 380 nm dose is the lowest (15%). Afterward, deposition increases with particle size, reaching 26% for 1000 nm. In the case of WEDM-UFPs, about 98% of all 1.0 nm inhaled particles remain in the lung. Subsequently, the deposition dose decreases with the particle size and reaches up to 28% for 100 nm particles. Approximately 51% of deposited WEDM-UFPs are of CMAD ≤ 5 nm. The images of lung geometry also observed the maximum deposited mass and mass flux rate in the head, tracheobronchial, and pulmonary airways.
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