Development of new approach methodologies is urgently needed to characterize the likelihood that complex mixtures of chemicals affect water quality. Omics advances in ecotoxicology allow assessment on a broadest coverage of disrupted biological pathway by mixtures. Here the usefulness of transcriptomic analyses for evaluation of combined effects and identification of main effect components are explored. Two artificial mixtures (Mix 1 and Mix 2) were tested by a concentration-dependent reduced zebrafish transcriptome (CRZT) approach and toxicity bioassays using zebrafish embryos. Then, the toxicities and transcriptomic effects of 12 component chemicals on embryos were incorporated into additivity models to characterize the combined effects of chemicals in mixtures and to identify the main bioactive compounds. Mix 1 and Mix 2 displayed similar embryo toxicities (LD₅₀: 6.6 μM and 8.7 μM, respectively), however, Mix 2 elicited broader biological process perturbations and 5-fold higher transcriptome potency (point of departure eliciting a 20% pathway response, PODₚₐₜₕ₂₀) than Mix 1. The predicted mixture toxicities derived from additivity expectations deviated by 2-fold or less from the measured embryo toxicities except for the Jaw defect endpoint; most biological processes deviated by 3-fold or less. Finally, diclofenac (DFC) and propiconazole (PCZ) were identified as the main contributing components (≥80% explanation) to the embryo toxicity and biological process perturbations by Mix 1. While DFC and chlorophene (CLP) explained up to 80% of the embryo toxicities and biological effects of Mix 2 associated with development and Metabolism processes. The CRZT approach provides a powerful tool for assessment of biological pathways perturbed by chemicals in mixtures and for identification of main bioactive compounds.

Fumonisins (FBs) are mycotoxins that are widely distributed in crops and feed, and ingestion of FBs -contaminated crops is harmful to animal health. Furthermore, it is unknown if Fumonisins B1 (FB1) can cause intestinal toxicity. To investigate FB1-induced intestinal toxicity, mice were treated with 0 or 5 mg/kg FB1 by gavage administration for 42 days. Histopathology indicated that FB1 exposure caused proliferation of intestinal epithelial cells, intestinal villi and epithelial layer shedding, intestinal gland atrophy, and necrosis. Notably, FB1 interfered with nuclear xenobiotic receptors (NXR) homeostasis by regulating the level of aryl hydrocarbon receptor (AHR), constitutive androstane receptor (CAR), pregnane X receptor (PXR) and downstream target genes (CYP450s). Moreover, abnormal expression of inflammatory cytokines (IL-1β, IL-2, IL-4, IL-10, and TNF-α) indicated the occurrence of inflammation. The present study provides new insights regarding the mechanism of FB1-induced intestinal toxicity through activating the NXR system and by triggering inflammatory responses in the intestinal tract in mice.

Oil-contaminated soils resulted from drilling activities can cause significant damages to the environment, especially for living organisms. Treatment and management of these soils are the necessity for environmental protection. The present study investigates the field study of seven oil-contaminated soils treated by different stabilization/solidification (S/S) methods, and the selection of the best treated site and treatment method. In this study, first, the ratios of consumed binders to the contaminated soils (w/w) and the treatment times for each unit of treated soils were evaluated. The ratios of consumed binders to the contaminated soils were between 6 and 10% and the treatment times for each unit of treated soils were between 4.1 and 18.5 min/m³. Physicochemical characteristics of treated soils were also determined. Although S/S methods didn’t change the water content of treated soils, they increased the porosity of soils. Unexpectedly, the cement-based S/S methods didn’t increase the pH of the treated soils. The highest and the lowest leaching of petroleum hydrocarbons was belonging to S/S using diatomaceous earth (DE) and the combination of Portland cement, sodium silicate and DE (CS-DE), respectively. The best acid neutralization capacity was obtained for soils treated using the combination of Portland cement and sodium silicate (CS). Based on the best-worst multi-criteria decision-making method (BWM-MCDM), the soils treated using CS-DE was select as the best. The BWM-MCDM can be used as an effective tool for the selection of the best alternative in all areas of environmental decontamination.

Natural media such as soil and sediment contain mineralogical and organic components with distinct chemical, surface, and electrostatic properties. To better understand the role of various soil and sediment components on particle transport, columns were packed with quartz sand and natural sediment with added Fe oxyhydroxide coating, illite clay, and peat moss to investigate how these added components influence nTiO₂ retention and transport in geochemically heterogeneous medium. Results showed that nTiO₂ transport was low at pH 5, attributable to the electrostatic attraction between positively-charged nTiO₂ and negatively-charged medium. While illite did not notably affect nTiO₂ transport at pH 5, Fe oxyhydroxide coating increased nTiO₂ transport due to electrostatic repulsion between Fe oxyhydroxide and nTiO₂. Peat moss also increased nTiO₂ transport at pH 5, attributable to the increased DOC concentration, which resulted in higher DOC adsorption to nTiO₂ and intensified electrostatic repulsion between nTiO₂ and the medium. At pH 9, nTiO₂ transport was high due to the electrostatic repulsion between negatively-charged nTiO₂ and medium surfaces. Fe oxyhydroxide coating at pH 9 slightly delayed nTiO₂ transport due to electrostatic attraction, while illite clay and peat moss substantially inhibited nTiO₂ transport via straining/entrapment or electrostatic attraction. Overall, this study demonstrated that pH has a considerable effect on how minerals and organic components of a medium influence nTiO₂ transport. At low pH, electrostatic attraction was the dominant mechanism, therefore, nTiO₂ mobility was low regardless of the differences in mineralogical and organic components. Conversely, nTiO₂ mobility was high at high pH and nTiO₂ retention was dominated by straining/entrapment and sensitive to the mineralogical and organic composition of the medium.

In this study, the YH consortium, an ethene-producing culture, was used to evaluate the effect of vitamin B₁₂ (VB₁₂) on trichloroethene (TCE) dechlorination by transferring the original TCE-reducing culture with or without adding exogenous VB₁₂. Ultra-high performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS) was applied to detect the concentrations of VB₁₂ and its lower ligand 5,6-dimethylbenzimidazole (DMB) in the cultures. After three successive VB₁₂ starvation cycles, the dechlorination of TCE stopped mostly at cis-dichloroethene (cDCE), and no ethene was found; methane production increased significantly, and no VB₁₂ was detected. Results suggest that the co-cultured microbes may not be able to provide enough VB₁₂ as a cofactor for the growth of Dehalococcoides in the YH culture, possibly due to the competition for corrinoids between Dehalococcoides and methanogens. The relative abundances of 16 S rRNA gene of Dehalococcoides and reductive dehalogenase genes tceA or vcrA were lower in the cultures without VB₁₂ compared with the cultures with VB₁₂. VB₁₂ limitation changed the microbial community structures of the consortia. In the absence of VB₁₂, the microbial community shifted from dominance of Chloroflexi to Proteobacteria after three consecutive VB₁₂ starvation cycles, and the dechlorinating genus Dehalococcoides declined from 42.9% to 13.5%. In addition, Geobacter, Clostridium, and Desulfovibrio were also present in the cultures without VB₁₂. Furthermore, the abundance of archaea increased under VB₁₂ limited conditions. Methanobacterium and Methanosarcina were the predominant archaea in the culture without VB₁₂.

Hexavalent chromium and nitrate co-contaminated groundwater remediation are attracting extensive attention worldwide. However, the transformation pathways of chromium and nitrate and the interplay mechanism between them remain unclear. In this work, zeolite-supported nanoscale zero-valent iron/palladium (Z-Fe/Pd) was synthesized and used for the first time to simultaneously remediate Cr(VI) and nitrate. Transmission electron microscopy, X-ray diffraction, and X-ray photoelectron spectroscopy analyses confirmed that nanoscale zero-valent iron/palladium was successfully loaded onto zeolite and it exhibited good dispersibility and oxidation resistance. Results of batch experiments showed that the Cr(VI) and nitrate removal efficiencies decreased from 95.5% to 91.5% to 45% and 73%, respectively, with the initial solution pH increasing from 3.0 to 8.0. The removal rates and efficiencies of Cr(VI) and nitrate under anoxic conditions were higher than those under open atmosphere because the dissolved oxygen diminished the electron selectivity toward the target pollutants. Moreover, the presence of Cr(VI) inhibited nitrate reduction by forming Fe(III)-Cr(III) hydroxide to impede electron transfer. Cr(VI) removal was promoted by nitrate, within limits, by balancing the consumption and generation rate of Fe₃O₄, which enhanced electron migration from the Fe(0) core to the external surface. The removal capacities of Cr(VI) and nitrate reached 121 and 95.5 mg g⁻¹, respectively, which were superior to the removal capacities of similar materials. Results of product identification, XRD, and XPS analyses of spent Z-Fe/Pd indicated that the reduction of Cr(VI) was accompanied by adsorption and co-precipitation, whereas the reduction of nitrate was catalyzed by the synergism of Fe(0) and Pd(0). An alternative to the simultaneous remediation of Cr(VI) and nitrate from groundwater under anoxic conditions is provided.

Whereas the adverse effects of vanadium released from smelting activities on soil microbial ecology have been widely recognized, little is known about spatiotemporal vanadium distribution and microbial community dynamics in typical contaminated sites. This study describes vanadium contents associated with health risk and microbial responses in both topsoil and subsoil during four consecutive seasons around an ongoing-production smelter in Panzhihua, China. Higher levels of vanadium concentration exceeding soil background value in China (82 mg/kg) were found close to the smelter. Vanadium concentrations decreased generally with the increase in distance to the smelter and depth below surface, as soil vanadium pollution is induced mainly by atmospheric deposition of vanadium bearing dust during smelting. Residual fraction was the predominated vanadium form in soils, with pronounced increase in bioavailable vanadium during rainfall period due to frequent drought-rewetting process. Topsoil close to the smelter exhibited significant contamination, inducing high probability of adverse health effects. Spatiotemporal vanadium distribution creates filtering effects on soil microorganisms, promoting metal tolerant genera in topsoil (e.g. Microvirga) and subsoil (e.g. Bacillus, Geobacter), which is the key in maintaining the community structure by promoting cooperative relation with other taxa. Our results reveal spatiotemporal vanadium distribution in soils at site scale with potential health risk and microbial responses, which is helpful in identifying severe contamination and implementing bioremediation.

Soluble iron (FeS) in aerosols contributes to free oxygen radical generation with implications for human health, and potentially catalyzes sulfur dioxide oxidation. It is also an important external source of micronutrients for ocean ecosystems. However, factors controlling FeS concentration and its contribution to total iron (FeT) in aerosols remain poorly understand. Here, FeS and FeT in PM₂.₅ was studied at four urban sites in eastern China from 21 to 31 December, 2017. Average FeT (869–1490 ng m⁻³) and FeS (24–68 ng m⁻³) concentrations were higher in northern than southern China cities, but Fe solubility (%FeS, 2.7–5.0%) showed no spatial pattern. Correlation analyses suggested %FeS was strongly correlated with FeS and PM₂.₅ instead of FeT concentrations. Individual particle observations confirmed that more than 65% of nano-sized Fe-containing particles were internally mixed with sulfates and nitrates. Furthermore, there was a high correlation between sulfates or nitrates/FeT molar ratio and %FeS. We also found that the sulfates/nitrates had weaker effects on %FeS at RH < 50% than at RH > 50%, suggesting RH as indirect factor can influence %FeS in PM₂.₅. These results suggest an important role of chemical processing in enhancing %FeS in the polluted atmosphere.

Coal-Gas replacement project has been implemented to decrease haze pollution in China in recent years. Airborne arsenic (As) mostly originates from coal burning processes. It is noteworthy to compare the distribution of arsenic fraction in PM₂.₅ before and after coal-gas replacement. Eighty PM₂.₅ samples were collected in Baoding in December 2016 (coal dominated year) and December 2017 (gas dominated year) at different functional areas including residential area (RA), industrial area (IA), suburb (SB), roadside (ST) and Botanical Garden Park (BG). The fraction, bioavailability and health risk of As in the PM₂.₅ samples were investigated and compared between these two years. Arsenic was mainly distributed in the non-specifically sorbed fraction (F1) and the residual fraction (F5). However, the proportion of F1 to the total As in 2017 was higher than that in 2016, while the proportion of As in the amorphous and poorly-crystalline hydrous oxides of Fe and Al fraction (F3) in 2017 was lower. The distributions of fraction and bioavailability showed temporal and spatial characteristics. The total concentration and bioavailability of As in SB and IA were significantly higher than those in RA, ST and BG. The BF (Bioavailability Factor) values of As ranged from 0.30 to 0.61. Health risk assessment indicated that the hazard quotient (HQ) and carcinogenic risk (CR) of As in PM₂.₅ significantly decreased after coal-gas replacement.

The present study attempts to explore and compare the seasonal variability in chemical composition and contributions of different sources of fine and coarse fractions of aerosols (PM₂.₅ and PM₁₀) in Delhi, India from January 2013 to December 2016. The annual average concentrations of PM₂.₅ and PM₁₀ were 131 ± 79 μg m⁻³ (range: 17–417 μg m⁻³) and 238 ± 106 μg m⁻³ (range: 34–537 μg m⁻³), respectively. PM₂.₅ and PM₁₀ samples were chemically characterized to assess their chemical components [i.e. organic carbon (OC), elemental carbon (EC), water soluble inorganic ionic components (WSICs) and heavy and trace elements] and then used for estimation of enrichment factors (EFs) and applied positive matrix factorization (PMF5) model to evaluate their prominent sources on seasonal basis in Delhi. PMF identified eight major sources i.e. Secondary nitrate (SN), secondary sulphate (SS), vehicular emissions (VE), biomass burning (BB), soil dust (SD), fossil fuel combustion (FFC), sodium and magnesium salts (SMS) and industrial emissions (IE). Total carbon contributes ∼28% to the total PM₂.₅ concentration and 24% to the total PM₁₀ concentration and followed the similar seasonality pattern. SN and SS followed opposite seasonal pattern, where SN was higher during colder seasons while SS was greater during warm seasons. The seasonal differences in VE contributions were not very striking as it prevails evidently most of year. Emissions from BB is one of the major sources in Delhi with larger contribution during winter and post monsoon seasons due to stable meteorological conditions and aggrandized biomass burning (agriculture residue burning in and around the regions; mainly Punjab and Haryana) and domestic heating during the season. Conditional Bivariate Probability Function (CBPF) plots revealed that the maximum concentrations of PM₂.₅ and PM₁₀ were carried by north westerly winds (north-western Indo Gangetic Plains of India).