The accuracy of the nitrogen (N) budget is of great importance for evidence-based decision-making to address both food security and environmental protection challenges. This study attempts to advance understanding of uncertainties in China's N budget using the Coupled Human And Natural Systems (CHANS) model and Monte Carlo simulation from 1980 to 2018. Results show that the spatial and temporal variations in agricultural and industrial activities and insufficient knowledge on N cycling parameterization are the two dominant causes of uncertainties in the N budget in China. Uncertainties of N inputs generally are <10%, while they are <30% for N outputs and >30% for N accumulations. Uncertainty of nitrogen oxides emission is more sensitive to energy consumption due to the large contributions from industry and transportation. While the uncertainty of ammonia emission is predominantly affected by agricultural activity. Combining surface measurements, satellite observations, and atmospheric simulation models enables cross-check of N fluxes in multiple systems and reduces uncertainties of N budget.

Freshwater salinization is a rapidly emerging ecological issue and is correlated with significant declines in aquatic biodiversity. It remains unclear how changing salinity regimes affect the physiology of sensitive aquatic insects. We used the parthenogenetic mayfly, Neocloeon triangulifer, to ask how ionic exposure history alters physiological processes and responses to subsequent major ion exposures. Using radiotracers (²²Na, ³⁵SO₄, and ⁴⁵Ca), we observed that mayflies chronically reared in elevated sodium or sulfate (157 mg L⁻¹ Na or 667 mg L⁻¹ SO₄) had 2-fold (p < 0.0001) and 8-fold (p < 0.0001) lower ion uptake rates than mayflies reared in dilute control water (16 mg L⁻¹ Na and 23 mg L⁻¹ SO₄) and subsequently transferred to elevated salinities, respectively. These acclimatory ion transport changes provided protection in 96-h toxicity bioassays for sodium, but not sulfate. Interestingly, calcium uptake was uniformly much lower and minimally influenced by exposure history, but was poorly tolerated in the toxicity bioassays. With qRT-PCR, we observed that the expression of many ion transporter genes in mayflies was influenced by elevated salinity in an ion-specific manner (general upregulation in response to sulfate, downregulation in response to calcium). Elevated sodium exposure had minimal influence on the same genes. Finally, we provide novel light microscopic evidence of histomorphological changes within the epithelium of the Malpighian tubules (insect primary excretory system) that undergoes cellular degeneration and necrosis secondary to calcium toxicity. We conclude that physiological plasticity to salinity stress is ion-specific and provide evidence for ion-specific toxicity mechanisms in N. triangulifer.

Road traffic emissions are an increasingly important source of particulate matter in urban and non-road environments, where non-tailpipe emissions can contribute substantially to elevated levels of metals associated with adverse health effects. Thus, better characterization and quantification of traffic-emitted metals is warranted. In this study, real-world emission factors for fine particulate metals were determined from hourly x-ray fluorescence measurements over a three-year period (2015–2018) at an urban roadway and busy highway. Inter-site differences and temporal trends in real-world emission factors for metals were explored. The emission factors at both sites were within the range of past studies, and it was found that Ti, Fe, Cu, and Ba emissions were 2.2–3.0 times higher at the highway site, consistent with the higher proportion of heavy-duty vehicles. Weekday emission factors for some metals were also higher by 2.0–3.5 times relative to Sundays for Mn, Zn, Ca, and Fe, illustrating a dependence on fleet composition and roadway activity. Metal emission factors were also inversely related to relative humidity and precipitation, due to reduced road dust resuspension under wetter conditions. Correlation analysis revealed groups of metals that were co-emitted by different traffic activities and sources. Determining emission factors enabled the isolation of traffic-related metal emissions and also revealed that human exposure to metals in ambient air can vary substantially both temporally and spatially depending on fleet composition and traffic volume.

In recent years, severe air pollution still frequently occurs in winter despite the effective implementation of clean air actions in China. Therefore, field measurements of particle composition and gas precursors were collected from December 1, 2018 to January 15, 2019 at an urban site in a central Chinese city to investigate the existing mechanisms of pollution. The hourly averaged PM₂.₅ concentration during the campaign was 92.7 μg m⁻³, with nitrate and organic aerosol (OA) demonstrated as the principal components. Generally, NO₂ oxidation in the daytime was observed as the major mechanism for nitrate generation, and aerosol water content (AWC) showed its influential role with the associated increases in the nitrogen oxidation and nitrate partitioning ratios. When AWC increased from dozens to hundreds of μg m⁻³ after the afternoon, nocturnal N₂O₅ hydrolysis was demonstrated as the overriding mechanism and provoked extreme contamination of nitrates. Five sources of organic aerosols (OAs) were identified: hydrocarbon-like OAs (HOAs, 16.5%), coal combustion OAs (CCOAs, 19.2%), biomass burning OAs (BBOAs, 9.9%), semi-volatile oxygenated OAs (SV–OOAs, 29.4%), and low-volatile oxygenated OAs (LV-OOAs, 25.0%). SV-OOAs and LV-OOAs were identified as gasSOAs and aqSOAs according to their sensitivities to the atmospheric oxidation capacity and AWC. In addition, aqueous-phase processing was found to be the dominant pathway for SOA formation when the AWC concentration was higher than 80 μg m⁻³. As an influential factor for nitrate and SOA formation, AWC could be greatly affected by RH and the concentrations of inorganic species. Sulfate, which was mainly contributed by anthropogenic emissions, was demonstrated to be a significant factor for active aqueous phase reactions, although SO₂ has been dramatically reduced in recent years. Above all, this study revealed the significant role of AWC in current pollution episode in winter, and will assist in establishing future measures for pollution mitigation.

Ammonia (NH₃) is an important precursor for forming PM₂.₅. In this study, we estimated the impact of upwind transboundary and local downwind NH₃ emissions on PM₂.₅ and its inorganic components via photochemical grid model simulations. Nine sensitivity scenarios with ±50% perturbations of upwind (China) and/or downwind (South Korea) NH₃ emissions were simulated for the year 2016 over Northeast Asia. The annual mean PM₂.₅ concentrations in the downwind area were predicted to change from −3.3 (−18%) to 2.4 μg/m3(13%) when the NH₃ emissions in the upwind and downwind areas were perturbed by -50% to +50%. The change in PM₂.₅ concentrations in the downwind area depending on the change in NH₃ emissions in the upwind area was the highest in spring, followed by winter. This was mainly attributed to the change in nitrate (NO₃⁻), a secondary inorganic aerosol (SIA) that is a predominant constituent of PM₂.₅. Since NH₃ is mainly emitted near the surface and vertical mixing is limited during the night, it was modeled that the aloft nitric acid (HNO₃)-to-NO₃⁻ conversion in the morning hours was increased when the NH₃ accumulated near the surface during nighttime begins to mix up within the Planetary Boundary Layer (PBL) as it develops after sunrise. This implies that the control of upwind and/or downwind NH₃ emissions is effective at reducing PM₂.₅ concentrations in the downwind area even under NH₃ rich conditions in Northeast Asia.

Inhaled polycyclic aromatic hydrocarbons (PAHs) can directly interact with the lung surfactant (PS) lining of alveoli, thereby affecting the normal physiological functions of PS, which is a serious threat to lung health. In spite of the extensive study of benzo[a]pyrene (BaP, a representative of PAHs), its potential biophysical influence on the natural PS is still largely unknown. In this study, the interfacial interaction between PS (extracted from porcine lungs) and BaP is investigated in vitro. The results showed that the surface tension, phase behavior, and interfacial structure of the PS monolayers were obviously altered in the presence of BaP. A solubilization test manifested that PS and its major components (dipalmitoyl phosphatidylcholine, DPPC; bovine serum albumin, BSA) could in turn accelerate the dissolution of BaP, which followed the order: PS > DPPC > BSA, and mixed phospholipids were significantly responsible for the solubilization of BaP by PS. In addition, solubilization of BaP also enhanced the consumption of hydroxyl radicals (·OH) in the simulated lung fluid, which could disturb the balance between oxidation and antioxidation.

PM₂.₅ in Shijiazhuang was collected from October 15, 2018 to January 31, 2019, and selected toxic elements were measured. Five typical haze episodes were chosen to analyze the health risks and critical risk sources. Toxic elements during the haze episodes accounted for 0.33% of PM₂.₅ mass. Non-cancer risk of toxic elements for children was 1.8 times higher than that for adults during the haze episodes, while cancer risk for adults was 2.5 times higher than that for children; cancer and non-cancer risks were primarily attributable to As and Mn, respectively. Health risks of toxic elements increased during the growth and stable periods of haze episodes. Non-cancer and cancer risks of toxic elements during the haze stable periods were higher than other haze stages, and higher for children than for adults during the stable period. Mn was the largest contributor to non-cancer risk during different haze stages, while As was the largest contributor to cancer risk. Crustal dust, vehicle emissions, and industrial emissions were critical sources of cancer risk during the clean-air periods; while vehicle emissions, coal combustion, and crustal dust were key sources of cancer risk during the haze episodes. Cancer risks of crustal dust and vehicle emissions during the haze episodes were 2.0 and 1.7 times higher than those in the clean-air periods. Non-cancer risks from emission sources were not found during different periods. Cancer risks of biomass burning and coal combustion increased rapidly during the haze growth period, while that of coal combustion decreased sharply during the dissipation period. Vehicle emissions, crustal dust, and coal combustion were significant cancer risk sources during different haze stages, cancer risk of each source was the highest during the stable period. Southern Hebei, Northern and central Shaanxi were potential risk regions that affected the health of both adults and children in Shijiazhuang.

We used real-world exposure scenarios to evaluate the effect of six ambient air pollutant (PM₂.₅, PM₁₀, NO₂, SO₂, CO, and O₃) exposure on renal function among older adults without chronic kidney disease (CKD). We recruited 169 older adults without CKD in Beijing, China, for a longitudinal study from 2016 to 2018. The Modification of Diet in Renal Disease (MDRD) and the Chronic Kidney Disease Epidemiology Collaboration (EPI) equations were employed to derive the estimated glomerular filtration rate (eGFR). A linear mixed-effects model with random intercepts for participants was employed to determine the effects of air pollutants on renal function evaluated on the basis of eGFR and urinary albumin/creatinine ratio at different exposure windows (1-, 2-, 3-, 5-, 7-, 14-, 28-, 45-, and 60-days moving averages). An interquartile range (IQR) increase in NO₂ for was associated with significant decreases of in eGFR (MDRD equation) [percentage changes: −4.49 (95% confidence interval: −8.44, −0.37), −5.51 (−10.43, −0.33), −2.26 (−4.38, −0.08), −3.71 (−6.67, −0.65), −5.44 (−9.58, −1.11), −5.50 (−10.24, −0.51), −6.15 (−10.73, −1.33), and −6.34 (−11.17, −1.25) for 1-, 2-, 5-, 7-, 14-, 28-, 45-, and 60-days moving averages, respectively] and in eGFR (EPI equation) [percentage changes: −5.04 (−7.09, −2.94), −6.25 (−8.81, −3.62), −5.16 (−7.34, −2.92), −5.10 (−7.85, −2.28), −5.83 (−8.23, −3.36), −6.04 (−8.55, −3.47) for 1-, 2-, 14-, 28-, 45-, and 60-days moving averages, respectively]. In two-pollutant model, only the association of NO₂ exposure with eGFR remained robust after adjustment for any other pollutant. This association was stronger for individuals with hypertension for the EPI equation or BMI <25 kg/m² for the MDRD equation at lags 1 and 1–2. Our findings suggest that NO₂ exposure is associated with eGFR reduction among older adults without CKD for short (1-, 2-days) and medium (14-, 28-, 45-, 60-days) term exposure periods; thus, NO₂ exposure may contribute to renal impairment.

To assess genetoxicity and the underlying mechanisms of carbamazepine (CBZ) toxicity in fish, adult Chinese rare minnows (Gobiocypris rarus) were exposed to 1, 10, and 100 μg/L CBZ for 28 d. Comet assays indicated that hepatic DNA damage was significantly increased in groups of minnows exposed to CBZ at all concentrations in a dose-dependent manner compared to those of the control groups (p < 0.05). Liver levels of 8-hydroxydeoxyguanosine (8-OHdG) were significantly increased at 10 and 100 μg/L CBZ (p < 0.05). TUNEL assays indicated that the average apoptotic rates of the livers of female and male minnows were significantly increased following exposure to CBZ at all concentrations for 28 d (p < 0.05). Significant increases in caspase 3 and 9 activities after CBZ exposure at all concentrations and caspase 8 at 10 and 100 μg/L CBZ exposure reflected the presence of mitochondrial apoptosis (p < 0.05). The mRNA levels of gadd45a, mdm2, casp3 and casp9 in female and male minnows exposed to CBZ at all concentrations were significantly increased compared with those in the control groups (p < 0.05). Significant increases in the levels of p21 in female minnows exposed to 1 and 100 μg/L CBZ, p53 in female minnows at all CBZ treatments and bcl2 in male minnows exposed to 1 and 100 μg/L CBZ were observed, indicating p53 pathway activation. The inhibition of ras levels in females and males exposed to CBZ at all concentrations and increased levels of raf1 in males exposed to CBZ at all concentrations indicated Ras/Raf1/MAPK (ERK) activation. Therefore, the present study demonstrates that CBZ at environmentally relevant levels induces DNA damage and apoptosis in Chinese rare minnows by the Ras/Raf/ERK/p53 signaling pathway.

Although estuaries act as a sink of many pollutants, the assessment of microplastics pollution in these transition environments remain scarce or unknown. Sediments from estuarine intertidal areas possess a great ecological concern but also could act as a sink of both buoyant and non-buoyant plastic debris. In this work, we have compared microplastic abundances and their main characteristics (size, shape, colour and polymer type) on intertidal sediments from SW Atlantic Argentinean estuaries (Samborombón, Mar Chiquita, Quequén Grande and Bahía Blanca). The mean microplastics abundance in surface sediments among estuaries was ranged from 0 to 1030 ± 657 items per kg d. w. Upper intertidal areas have shown significant higher microplastic abundances in comparison with lower intertidal zones (p < 0.05). In general, higher mean abundances were observed in surface (0–10 cm) compared to deeper sediments (10–30 cm), which revealed recent deposition trends of microplastics particles. However, higher microplastic abundances were observed in deeper sediments from Bahía Blanca (p < 0.05). Large (MPs, 1–5 mm) and small microplastics (MMPs, 1–0.1 mm) were observed in sediments from all estuaries, representing from 17 to 100% of total microplastics abundance. In terms of shape, fragments and films forms were dominant in all samples while fibers and foams were moderately or poorly represented. White, black, blue and transparent particles were observed as the main colour contribution of microplastics in sediments. The most common polymers identified included polyethylene, polyethylene terephthalate/polyester, polyvinyl chloride and polypropylene. The results showed that microplastic characteristics are different among the estuarine environments, which suggests different anthropogenic sources. Besides, deeper sediments should also be included on intertidal sediment monitoring to identify potential environmental liabilities from anthropogenically impacted estuaries.