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النتائج 581 - 590 من 4,896
Statistical determination of crucial taxa indicative of pollution gradients in sediments of Lake Taihu, China
2019
Li, Yi | Wu, Hainan | Shen, Yun | Wang, Chao | Wang, Peifang | Zhang, Wenlong | Gao, Yu | Niu, Lihua
In order to accurately monitor the changes in a freshwater ecosystem in response to anthropogenic stressors, microbe–environment correlations and microbe–microbe interactions were combined to determine crucial indicator taxa in contaminated sediments. The diversity, composition, and co–occurrence pattern of bacterial communities in 23 sediment samples collected from Lake Taihu were explored using 16S rRNA amplicon sequencing analysis. Fisher's exact test showed that the cluster analyses of samples could show a direct correlation between the relative abundance of bacterial communities and the physicochemical properties of the sediment (P < 0.0001), suggesting that bacterial communities can be used to monitor contamination gradients in freshwater sediments. According to the microbe–environment correlation, 24 orders and 60 families were initially identified via indicator species analysis as indicator taxa of different pollution levels. The co–occurrence network further showed that topological features of bacterial communities were clearly different at different pollution levels, although the diversity and composition of bacterial communities displayed similarities between minimally and moderately polluted sites. Indicator taxa were then screened for keystone species, which co–occurrence relationships showed the high degree and low betweenness centrality values (i.e. degree >5, betweenness centrality <1000) of the network. Nine orders and 13 families were finally extracted as crucial indicator taxa of the different pollution levels in eutrophic Lake Taihu. Obtaining crucial indicator taxa from environmental sequences allows to trace increasing levels of pollution in aquatic ecosystems and provides a novel mean to monitor watersheds sensitive to anthropic influences.
اظهر المزيد [+] اقل [-]Accumulation, ecological-health risks assessment, and source apportionment of heavy metals in paddy soils: A case study in Hanzhong, Shaanxi, China
2019
Xiao, Ran | Guo, Di | Ali, Amjad | Mi, Shenshen | Liu, Tao | Ren, Chunyan | Li, Ronghua | Zhang, Zengqiang
Contamination of agricultural soil by heavy metals has become a global issue concerning food security and human health risk. In this study, a soil investigation was conducted to evaluate metals accumulation, potential ecological and health risks as well as to identify sources of metals in paddy soils in Hanzhong City, which is located in a sedimentary basin. Ninety-two (92) surface soil samples (bulk soil) and their corresponding rice samples, 21 irrigation water samples, and 18 fertilizer samples were collected from two typical counties and quantified for the heavy metals (i.e., As, Cd, Cu, Hg, Pb, and Zn) concentrations. The results showed that As, Cd, and Zn were the main contaminants in soils in the studied area. Additionally, elevated Hg content in soils might also pose risks to the local ecosystem. Cadmium and As demonstrated high mobility, and their average contents in rice grains were slightly higher than the permissible threshold (0.20 mg kg⁻¹). Moreover, Pb, As, and Cd intake via rice consumption might result in potential risks to local residents. Metal distribution revealed that pollution in the studied area is non-homogeneous, and agricultural activities (As, Cu, and Cd), transportation emission (Cu and Pb), coal combustion (Hg and As), and smelting activities (Zn, Pb, and Cu) were ascertained as the potential sources based on the Positive matrix factorization (PMF) analysis results.
اظهر المزيد [+] اقل [-]Response of bleached and symbiotic sea anemones to plastic microfiber exposure
2019
Romanó de Orte, Manoela | Clowez, Sophie | Caldeira, K. (Ken)
Microplastics are emerging contaminants in the marine environment. They enter the ocean in a variety of sizes and shapes, with plastic microfiber being the prevalent form in seawater and in the guts of biota. Most of the laboratory experiments on microplastics has been performed with spheres, so knowledge on the interactions of microfibers and marine organisms is limited. In this study we examined the ingestion of microfibers by the sea anemone Aiptasia pallida using three different types of polymers: nylon, polyester and polypropylene. The polymers were offered to both symbiotic (with algal symbionts) and bleached (without algal symbionts) anemones. The polymers were introduced either alone or mixed with brine shrimp homogenate. We observed a higher percentage of nylon ingestion compared to the other polymers when plastic was offered in the absence of shrimp. In contrast, we observed over 80% of the anemones taking up all types of polymers when the plastics were offered in the presence of shrimp. Retention time differed significantly between symbiotic and bleached anemones with faster egestion in symbiotic anemones. Our results suggest that ingestion of microfibers by sea anemones is dependent both on the type of polymers and on the presence of chemical cues of prey in seawater. The decreased ability of bleached anemones to reject plastic microfiber indicates that the susceptibility of anthozoans to plastic pollution is exacerbated by previous exposure to other stressors. This is particularly concerning given that coral reef ecosystems are facing increases in the frequency and intensity of bleaching events due to ocean warming.
اظهر المزيد [+] اقل [-]The accumulation and distribution of five antibiotics from soil in 12 cultivars of pak choi
2019
Yu, Xiaolu | Liu, Xiaoxia | Liu, Hang | Chen, Junhao | Sun, Ying
There is a lack of understanding about the potential accumulation of antibiotics in plants exposed to low-dose contaminated soil. 12 Brassica rapa subsp. chinensis cultivars were used to investigate the different accumulation capacities of sulfamethoxypyridazine, tetracycline, ofloxacin, norfloxacin and difloxacin from the soil. The results showed a significant variation (p < 0.05) among the 12 cultivars in the accumulation of antibiotics. Cultivars Y1 and Y2 had the highest accumulation capacity with average concentrations of 3.26 and 3.00 μg kg⁻¹, respectively, while cultivars Y4 and Y9 had the lowest accumulation capacity with average concentrations of 0.83 and 0.89 μg kg⁻¹. The average antibiotic concentration in all edible part samples (2.74 μg kg⁻¹) of the treatment group was about 3.0-fold of that of the control group (0.93 μg kg⁻¹). The average bioconcentration factors of sulfamethoxypyridazine, tetracycline, ofloxacin, norfloxacin and difloxacin were 0.051, 0.031, 0.017, 0.036 and 0.034, respectively, indicating a higher uptake of sulfamethoxypyridazine compared to ofloxacin. And the mobility of antibiotics in soil is a main factor affecting the bioavailability for plants. The average concentration of antibiotics in edible parts of cultivar Y12 on the 25th and 45th day were 1.52 and 1.73 μg kg⁻¹ and that of the roots were 3.73 and 6.61 μg kg⁻¹, respectively. The concentrations of tetracycline and difloxacin in the edible parts and roots significantly increased with growing time, while the concentration of sulfamethoxypyridazine and ofloxacin changed little throughout the growing period. The potential risks of antibiotics in vegetables on human health cannot be ignored. Overall, attention should be paid to the translocation of antibiotics from soil to plants.
اظهر المزيد [+] اقل [-]Acute and chronic effects of polystyrene microplastics on juvenile and adult Daphnia magna
2019
Eltemsah, Yehia Sayed | Bohn, Thomas
We investigate the distribution and effects of polystyrene microplastic (MP) particles in exposure experiments with the ecotoxicology model organism Daphnia magna. The aim was to investigate the short and long-term toxicity of MP at different concentrations. To achieve this goal, the effects of 6 μm commercially available polystyrene beads on two different life-stages of D. magna: < 24 h old juveniles and 9 days old adults was assessed. The following end points in test animals were measured: (1) survival, (2) growth, (3) individual and population fecundity, (4) age at maturation and (5) body size of newborn offspring. These response variables were followed in two acute and two chronic experiments. The acute experiments showed that MP is not acutely toxic to D. magna within 48 h, but cause added mortality within 120 h. The juveniles were about 50% more sensitive than the adults tested. In life-cycle experiments testing chronic exposure to MP, again, animals exposed as juveniles at relatively high concentrations, i.e. > 30 μg ml⁻¹ showed higher sensitivity. We observed slightly increased mortality, reduced growth and stimulation of early reproduction at the cost of later reproduction. Animals exposed after reaching adulthood did not show increased mortality and showed a stimulation response with higher reproductive rates than the control group. However, both the growth rate of mother animals and the body size of newborn declined with increasing dose of MP. We conclude that these effects indicate a role of MP in mechanical interaction/interference with the animal on the level of feeding (clogging filtering functions), digestion (gut filled with plastic particles), and/or other animal behavior. The study also illustrates how MP with slow break-down rates may accumulate in the environment and enter the food-chain as obstructing non-food particles in filter-feeding organisms.
اظهر المزيد [+] اقل [-]Endogenous cycles, activity patterns and energy expenditure of an intertidal fish is modified by artificial light pollution at night (ALAN)
2019
Pulgar, José | Zeballos, Danae | Vargas Ruiz, Juan Carlos | Aldana, Marcela | Manriquez, Patricio H. | Manriquez, Karen | Quijón, Pedro A. | Widdicombe, Stephen | Anguita, Cristobal | Quintanilla, Diego | Duarte, Cristian
The increase of global light emissions in recent years has highlighted the need for urgent evaluation of their impacts on the behaviour, ecology and physiology of organisms. Numerous species exhibit daily cycles or strong scototaxic behaviours that could potentially be influenced if natural lighting conditions or cycles are disrupted. Artificial Light Pollution at Night (ALAN) stands for situations where artificial light alters natural light-dark cycles, as well as light intensities and wavelengths. ALAN is increasingly recognized as a potential threat to biodiversity, mainly because a growing number of studies are demonstrating its influence on animal behaviour, migration, reproduction and biological interactions. Most of these studies have focused on terrestrial organisms and ecosystems with studies on the effects of ALAN on marine ecosystems being more occasional. However, with the increasing human use and development of the coastal zone, organisms that inhabit shallow coastal or intertidal systems could be at increasing risk from ALAN. In this study we measured the levels of artificial light intensity in the field and used these levels to conduct experimental trials to determine the impact of ALAN on an intertidal fish. Specifically, we measured ALAN effects on physiological performance (oxygen consumption) and behaviour (activity patterns) of “Baunco” the rockfish Girella laevifrons, one of the most abundant and ecologically important intertidal fish in the Southeastern Pacific littoral. Our results indicated that individuals exposed to ALAN exhibited increased oxygen consumption and activity when compared with control animals. Moreover, those fish exposed to ALAN stopped displaying the natural (circatidal and circadian) activity cycles that were observed in control fish throughout the experiment. These changes in physiological function and behaviour could have serious implications for the long-term sustainability of fish populations and indirect impacts on intertidal communities in areas affected by ALAN.
اظهر المزيد [+] اقل [-]Nuclear receptor AHR-mediated xenobiotic detoxification pathway involves in atrazine-induced nephrotoxicity in quail (Coturnix C. coturnix)
2019
Zhang, Cong | Li, Huixin | Qin, Lei | Ge, Jing | Qi, Zhang | Talukder, Milton | Li, Yan-Hua | Li, Jin-Long
Atrazine (ATR), one of the most widely used pesticides in agricultural production, are gradually concerned due to potential ecosystem and health risks. Further, the induction of ATR nephrotoxicity and detoxification response is still unknown. To evaluate ATR-induced nephrotoxicity, quails were treated with 0, 50, 250 or 500 mg/kg ATR by gavage administration for 45 days. Histopathology indicated that ATR exposure caused renal tubular epithelial cell swelling and endoplasmic reticulum degeneration, suggesting that ATR exposure causes renal impairment even renal diseases. Notably, ATR interfered cytochrome P450 system (CYP450s) homeostasis by enhancing contents or activities of CYP450s (total CYP450, Cyt b5, AH, APND, NCR and ERND) and the expression of CYP450 isoforms (CYP1A, CYP1B, CYP2C and CYP3A). ATR triggered phase II detoxifying reaction, reflected by the elevated GSH level, GST activity and the up-regulation of GST isoforms (GSTa, GSTa3 and GSTt1) and GSH synthetase (GCLC). Moreover, ABC transporters were activated to expel ATR from the body by increasing expression of MRP1 and P-GP gene. Accompanying these alterations, the nuclear receptors (AHR, CAR and PXR) were activated by ATR in a dose-dependent manner. Analysis results of present study demonstrated that the induction of phase II detoxifying enzyme system and ABC transporters could be modulated by nuclear receptors response and CYP450s disturbance in low-dose ATR-treated quail. In conclusion, all data suggested that nuclear receptors AHR-mediated detoxification pathway was involved in ATR-induced nephrotoxicity. These results provided new evidence about the nephrotoxic effects of ATR on the response of biotransformation and detoxification system.
اظهر المزيد [+] اقل [-]Transcriptional responses to starvation stress in the hepatopancreas of oriental river prawn Macrobrachium nipponense
2019
Li, Fajun | Fu, Chunpeng | Xie, Yannian | Wang, Aili | Li, Jianyong | Gao, Junping | Cui, Xinyu
Various crustaceans are farmed using aquaculture, and food deprivation or fasting can occur due to changing of environmental or management strategies. However, the molecular mechanisms underlying responses to starvation in crustaceans remain unclear. To address this, 12 hepatopancreas transcriptomes were compared for oriental river prawn (Macrobrachium nipponense) from four fasting stages (0, 7, 14 and 21 d). Gene Ontology functional annotation and Kyoto Encyclopaedia of Genes and Genomes pathway enrichment analysis of differentially expressed genes were subsequently performed. During the early stages of starvation (0–7 d), drug metabolism via the cytochrome P450 pathway and metabolism of xenobiotics by the cytochrome P450 pathway were enriched, suggesting that they metabolised compounds generated under starvation stress. As starvation proceeded (7–14 d), the retinol (vitamin A) metabolism pathway was activated, based on three up-regulated genes (CYP3, ADH and UGT), along with the two p450 pathways. Meanwhile, vitamin A was gradually consumed. As acute starvation was reached (14–21 d), vitamin A deficiency decreased the mRNA expression levels of IGF-I that is involved in the mTOR signalling pathway, which ultimately affected the growth and development of M. nipponense. Our results implicate drug/xenobiotic metabolism by cytochrome P450s in adaptation to starvation stress. Furthermore, metabolic cascades (CYP and retinol pathways) and growth (mTOR signalling) pathways are clearly triggered in crustaceans during starvation. The findings expand our understanding of the genes associated with hepatopancreas functioning in M. nipponense, and the underlying molecular mechanisms that govern the responses of crustaceans to starvation stress.
اظهر المزيد [+] اقل [-]A methodological framework for identifying potential sources of soil heavy metal pollution based on machine learning: A case study in the Yangtze Delta, China
2019
Jia, Xiaolin | Hu, Bifeng | Marchant, Ben P. | Zhou, Lianqing | Shi, Zhou | Zhu, Youwei
It is a great challenge to identify the many and varied sources of soil heavy metal pollution. Often little information is available regarding the anthropogenic factors and enterprises that could potentially pollute soils. In this study we use freely available geographical data from a search engine in conjunction with machine learning methodologies to identify and classify potentially polluting enterprises in the Yangtze Delta, China. The data were classified into 31 separate and four integrated industry types by five different machine learning approaches. Multinomial naive Bayesian (NB) methods achieved an accuracy of 87% and Kappa coefficient of 0.82 and were used to classify the geographic data from more than 260,000 enterprises. The relationship between the different industry classes and measurements of soil cadmium (Cd) and mercury (Hg) concentrations was explored using bivariate local Moran's I analysis. The analysis revealed areas where different industry classes had led to soil pollution. In the case of Cd, elevated concentrations also occurred in some areas because of excessive fertilization and coal mining. This study provides a new approach to investigate the interaction between anthropogenic pollution and natural sources of soil heavy metals to inform pollution control and planning decisions regarding the location of industrial sites.
اظهر المزيد [+] اقل [-]Trends in surface water chemistry in afforested Welsh catchments recovering from acidification, 1991–2012
2019
Broadmeadow, S.B. | Nisbet, T.R. | Forster, J.
A key criterion of the UK Government's policy on sustainable forest management is safeguarding the quality and quantity of water. Forests and forestry management practices can have profound effects on the freshwater environment. Poor forest planning or management can severely damage water resources at great cost to other water users; in contrast good management that restores and maintains the natural functions of woodland can benefit the whole aquatic ecosystem.Forests and forest management practices can affect surface water acidification. Monitoring of water chemistry in ten forest and two moorland acid-sensitive catchments in upland Wales commenced in 1991. The streams were selected to supplement the United Kingdom Upland Waters Monitoring Network (UWMN) with additional examples of afforested catchments. Analysis of 22 years of water chemistry data revealed trends indicative of recovery from acidification. Excess sulphate exhibited a significant coherent decline, accompanied by increases in pH and “charge-balance based” acid neutralising capacity (CB-ANC). Alkalinity and “alkalinity-based” acid neutralising capacity (AB-ANC) exhibited fewer trends, possibily due to the variable responses of the organic - carbonate species to increasing pH in these low alkalinity streams. Whilst total anthropogenic acidity declined, dissolved organic carbon and Nitrate-Nitrogen (NNO₃) concentrations have risen, and the contribution of NNO₃ to acidification has increased.Between-stream variability was analysed using Principal Component Analysis of the trend slopes. Hierarchical clustering of the changes in stream water chemistry indicated three distinct clusters with no absolute distinction between moorland and forest streams. Redundancy analysis was used to test for significant site-specific variables that explained differences in the trend slopes, with rainfall, crop age, base cation concentration and forest cover being significant explanatory variables.
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