Plastic, and especially microplastic, contamination of soils has become a novel research field. After the detection of microplastics in soils, spatial distribution and dynamics are still unknown. However, the potential risks associated with plastic particles in soils cannot be sufficiently assessed without knowledge about the spatial distribution of these anthropogenic materials. Based on a spatial research approach, including soil surveys, this study quantified the mesoplastic (MEP, > 5.0 mm) and coarse microplastics (CMP, 2.0–5.0 mm) content of twelve floodplain soils. At four transects in the catchment area of the Lahn river (Germany), soils down to a depth of 2 m were examined for plastic content for the first time. MEP and CMP were detected through visual examination after sample preprocessing and ATR-FTIR analyses. Average MEP and CMP concentrations range between 2.06 kg⁻¹ (±1.55 kg⁻¹) and 1.88 kg⁻¹ (±1.49 kg⁻¹) with maximal values of 5.37 MEP kg⁻¹ to 8.59 CMP kg⁻¹. Plastic particles are heterogeneously distributed in samples. Both plastic size classes occur more frequently in topsoils than in soil layers deeper than 30 cm. The maximal depth of CMP occurrence lies between 75 and 100 cm. Most common CMP polymer type was PE-LD, followed by PP and PA. MEP and CMP particles occur frequently at near channel sides and more often on riparian strips or grassland than on farmland. Vertical distribution of CMP indicates anthropogenic relocation in topsoils and additional deep displacement through natural processes like preferential flow paths or bioturbation. By comparing sedimentation rates of the river with the maximum age of plastic particles, sedimentation as a deposition process of plastic in floodplains becomes probable. From our findings, it can be concluded that an overall widespread but spatial heterogenous contamination occurs in floodplain soils. Additionally, a complex plastic source pattern seems to appear in floodplain areas.

Biochar sequesters cadmium (Cd) by immobilisation, but the process is often less effective in field trials than in the laboratory. Therefore, the involvement of soil components should be considered for predicting field conditions that could potentially improve this process. Here, we used biochar derived from Spartina alterniflora as the amendment for Cd-contaminated soil. In simulation trials, a mixture of kaolin, a representative soil model component, and S. alterniflora-derived biochar immobilised Cd by forming silicon-aluminium-Cd-containing complexes. Interestingly, the biochar recalcitrance index value increased from 48% to 53%–56% because of the formation of physical barriers consisting of kaolinite minerals and Cd complexes. Pot trials were performed using Brassica chinensis for evaluating the effect of S. alterniflora-derived biochar on plant growth in Cd-contaminated soil. The bio-concentration factor values in B. chinensis were 24%–31% after soil remediation with biochar than in control plants. In summary, these results indicated that soil minerals facilitated Cd sequestration by biochar, which reduced Cd bioavailability and improved the recalcitrance of this soil amendment. Thus, mechanisms for effective Cd remediation should include biochar-soil interactions.

Heavy metal pollution is widespread, and has an increasing trend in some countries and regions. It can be easily accumulated in plants, leading to plant species loss and affecting plant community composition. Artificial restoration can conserve plant diversity in contaminated soils and accelerate the recovery of polluted ecosystems. The application of nitrogen (N) and phosphorus (P) is inexpensive and convenient, which can increase the resistance of plants to adversity and promote the growth of plants in heavy metal polluted soils. In order to examine the effect of N and P nutrition on the conservation of plant community, we conducted a comparison experiment in greenhouse using soil with low N and P concentration, and set five treatments: C (soil with no heavy metals and fertilizer addition), H (soil with heavy metals addition but with no fertilizer), HN (soil with heavy metals and N addition), HP treatment(soil with heavy metals and P addition), HNP treatment (soil with heavy metals, N and P addition). Our results showed that heavy metal pollution reduced plant species by 300%, and significantly decreased plant diversity (P < 0.05). N addition increased the richness of plant species and increased the dominance of Euphorbia peplus, but had no significant effect on plant diversity and community structure, while reduced the evenness of plant species. P addition of HP and HNP treatments restored plant species richness and increased plant diversity under heavy metal pollution. The plant community structures of these two treatments were more similar to that of group C. Compared with N addition, P addition had a better performance to restoring the species composition and relative dominance of plant communities. Our results provided a guidance for the restoration of plant communities and the conservation of plant species in low N and P concentration soils with the context of heavy metal pollution.

The excessive application of manure has caused a high load of phosphorus (P) in the North China Plain. Having an understanding of how manure application affects soil P changes and its transport between different soil layers is crucial to reasonably apply manure P and reduce the associated loss. Based on our 28-year field experiments, the compositions and changes of P species and the risk of P loss under excessive manure treatments were investigated, i.e., no fertilizer (CK), mineral fertilizer NPK (NPK), NPK plus 22.5 t ha⁻¹ yr⁻¹ swine manure (LMNPK), and NPK plus 33.75 t ha⁻¹ yr⁻¹ swine manure (HMNPK). Manure application increased the content of orthophosphate and myo-inositol hexaphosphate (myo-IHP), especially the orthophosphate content exceeded 95%. The amount of orthophosphate in manure and the conversion of organic P to inorganic P in soil were the main reasons for the increased soil orthophosphate. Compared with NPK treatment, soil microbial biomass phosphorus and alkaline phosphatase activity in LMNPK and HMNPK treatments significantly increased. Compared with NPK treatment, a high manure application rate under HMNPK treatment could increase the abundance of organic P-mineralization gene phoD by 60.0% and decrease the abundance of inorganic P-solubilization gene pqqC by 45.9%. Due to the continuous additional manure application, soil P stocks significantly increased under LMNPK and HMNPK treatments. Furthermore, part of the P has been leached to the 60–80 cm soil layer. Segmented regression analysis indicated that CaCl₂–P increased sharply when Olsen-P was higher than 25.1 mg kg⁻¹, however the content of Olsen-P did not exceed this value until 10 years after consecutive excessive manure application. In order to improve soil P availability and decrease the risk of P loss, the manure application rate should vary over time based on soil physicochemical conditions, plants requirements, and P stocks from previous years.

We investigated the influence of sulfate (SO₄²⁻) deposition and concentrations on the net formation and solubility of methylmercury (MeHg) in peat soils. We used data from a natural sulfate deposition gradient running 300 km across southern Sweden to test the hypothesis posed by results from an experimental field study in northern Sweden: that increased loading of SO₄²⁻ both increases net MeHg formation and redistributes methylmercury (MeHg) from the peat soil to its porewater. Sulfur concentrations in peat soils correlated positively with MeHg concentrations in peat porewater, along the deposition gradient similar to the response to added SO₄²⁻ in the experimental field study. The combined results from the experimental field study and deposition gradient accentuate the multiple, distinct and interacting roles of SO₄²⁻ deposition in the formation and redistribution of MeHg in the environment.

A typical two-day start-up of municipal solid waste incinerators (MSWIs) can yield polychlorinated dibenzo-p-dioxin and dibenzofuran (PCDD/F) and polychlorinated biphenyl (PCB) emission quantities around 10 times higher than those from an entire year of normal operations, as measured in this study. Thus, we tested specific control strategies for inhibiting the formation of chlorinated persistent organic pollutants (Cl-POPs), namely, extensively cleaning the ash accumulated beneath the furnace bed of the combustion chamber and deposited on the walls of the superheater and economizer and shortening the residence time of the flue gas in the optimal temperature window for Cl-POP formation. Also, we advanced the injection times of the activated carbon and lime slurry to lower Cl-POP emissions during start-up. Our findings show that these strategies were highly effective and reduced the Cl-POP emissions by > 98%, most of which (96.4–98.2%) was attributable to inhibiting formation. In summary, the proposed control strategies require no modifications to existing air pollution control devices, have little influence on operational cost, and are effective and feasible for the majority of MSWIs.

Urban areas are inherently noisy, and this noise can disrupt biological processes as diverse as communication, migration, and reproduction. We investigated how exposure to urban noise affects sleep, a process critical to optimal biological functioning, in Australian magpies (Cracticus tibicen). Eight magpies experimentally exposed to noise in captivity for 24-h spent more time awake, and less time in non-rapid eye movement (non-REM) and REM sleep at night than under quiet conditions. Sleep was also fragmented, with more frequent interruptions by wakefulness, shorter sleep episode durations, and less intense non-REM sleep. REM sleep was particularly sensitive to urban noise. Following exposure to noise, magpies recovered lost sleep by engaging in more, and more intense, non-REM sleep. In contrast, REM sleep showed no rebound. This might indicate a long-term cost to REM sleep loss mediated by noise, or contest hypotheses regarding the functional value of this state. Overall, urban noise has extensive, disruptive impacts on sleep composition, architecture, and intensity in magpies. Future work should consider whether noise-induced sleep restriction and fragmentation have long-term consequences.

Microcystis aeruginosa (MA) is a primary hazardous cyanobacteria species in aquatic ecosystems that can produce microcystin-LR (MC-LR), which harms aquatic animals. The intestine is an important target tissue for MA and MC-LR. In this study, we investigated the effects of MA and MC-LR exposure on the intestinal microbiota variation and immune responses of Litopenaeus vannamei. Shrimp were experimentally exposed to MA and MC-LR for 72 h. The results showed that both MA and MC-LR exposure caused marked histological variation and apoptosis characteristics and increased oxidative stress in the intestine. Furthermore, the relative expression levels of antimicrobial peptide genes (ALF, Crus, Pen-3) decreased, while those of pro-inflammatory cytokines (MyD88, Rel, TNF-a), a pattern-recognition receptor (TLR4) and a mediator of apoptosis (Casp-3) increased. MA and MC-LR exposure also caused intestinal microbiota variation, including decreasing microbial diversity and disturbing microbial composition. Specifically, the relative abundance of Proteobacteria decreased in the two stress groups; that of Bacteroidetes decreased in the MA group but increased in the MC-LR group, while Tenericutes varied inversely with Bacteroidetes. Our results indicate that MA and MC-LR exposure causes intestinal histopathological and microbiota variations and induces oxidative stress and immune responses in L. vannamei. In conclusion, this study reveals the negative effects of MA and MC-LR on the intestinal health of shrimp, which should be considered in aquaculture.

Mono-(2-ethylhexyl) phthalate (MEHP) is the primary monoester transformation product of the commonly used plasticizer, di-2-ethylhexyl phthalate (DEHP), and has been frequently detected in various environmental compartments (e.g., soil, biosolids, plants). Plants growing in contaminated soils can take up MEHP, and consumption of the contaminated plants may result in unintended exposure for humans and other organisms. The metabolism of MEHP in plants is poorly understood, but critical for evaluating the potential human and environmental health risks. The present study represents the first attempt to explore the metabolic fate of MEHP in plants. We used Arabidopsis thaliana cells as a plant model and explored metabolic pathways of MEHP using deuterium stable isotope labelling (SIL) coupled with time-of-flight high resolution mass spectrometer (TOF-HRMS). A. thaliana rapidly took up MEHP from the culture medium and mediated extensive metabolism of MEHP. Combining SIL with TOF-HRMS analysis was proved as a powerful method for identification of unknown MEHP metabolites. Four phase Ⅰ and three phase Ⅱ metabolites were confirmed or tentatively identified. Based on the detected transformation products, hydroxylation, oxidation, and malonylation are proposed as the potential MEHP metabolism pathways. In cells, the maximum fraction of each transformation product accounted for 2.8–56.5% of the total amount of metabolites during the incubation. For individual metabolites, up to 2.9–100% was found in the culture medium, suggesting plant excretion. The results in the cell culture experiments were further confirmed in cabbage and A. thaliana seedlings. The findings suggest active metabolism of MEHP in plants and highlight the need to include metabolites in refining environmental risk assessment of plasticizers in the agro-food systems.