خيارات البحث
النتائج 611 - 620 من 6,473
Toxic effects and mechanisms of three commonly used fungicides on the human colon adenocarcinoma cell line Caco-2
2020
Tao, Huaping | Bao, Zhiwei | Jin, Cuiyuan | Miao, Wenyu | Fu, Zhengwei | Jin, Yuanxiang
Fungicides, usually refer to the chemical agents that can effectively control or kill the pathogenic microorganisms. Here, we revealed the effects of three different fungicides, imazalil (IMZ), chlorothalonil (CTL) and carbendazim (CBZ), which are typical broad-spectrum fungicides that are detected at high levels in the natural environment, on heterogeneous human epithelial colorectal cells (Caco-2 cells). All three fungicides had the potential to induce different degrees of toxicity, cause apoptosis, reactive oxygen species (ROS) and even change the cell cycle in the cells. The half maximal inhibitory concentration (IC50) of CTL is the lowest among these three fungicides, suggesting that it may have the highest exposure risk, followed by IMZ, and CBZ. The results of the real-time PCR, Western blotting, and mitochondrial membrane potential (MMP) assays and the activities of key enzymes suggested that CTL induced apoptosis in Caco-2 cells via a mitochondrial-dependent pathway, as indicated by the upregulation of the expression of the apoptotic p53 and bax genes, the increase of the apoptosis marker cytochrome-c, the decrease of mRNA level of bcl-2 gene, and the decrease in the MMP. Exposure to two other fungicides also upregulated the transcriptional level of bax and the expression of cytochrome-c, but the mRNA level of bcl-2 was increased (IMZ) or unchanged (CBZ), suggesting that other pathways may be involved in the induction of cellular apoptosis by these two fungicides. In addition, all three of the fungicides could induce oxidative stress in Caco-2 cells. Our data showed that the three different kinds of fungicides all caused toxic effects in Caco-2 cells through various pathways.
اظهر المزيد [+] اقل [-]Dibutyl phthalate contamination accelerates the uptake and metabolism of sugars by microbes in black soil
2020
Chen, Wenjing | Wang, Zhigang | Xu, Weihui | Tian, Renmao | Zeng, Jin
Dibutyl phthalate (DBP) is widely used as plasticizer and has been detected in the environment, posing a threat to animal health. However, the effects of DBP on agricultural microbiomes are not known. In this study, DBP levels in black soil were evaluated, and the impact of DBP contamination on the uptake and metabolism of sugars in microbes was assessed by glucose absorption tests, metaproteomics, metabolomics, enzyme activity assays and computational simulation analysis. The results indicated that DBP contamination accelerated glucose consumption and upregulated the expression of porins and periplasmic monosaccharide ATP-binding cassette (ABC) transporter solute-binding proteins (SBPs). DBP and its metabolic intermediates (carboxymuconate and butanol) may form a stable complex with sugar transporters and enhance the rigidity and stability of these proteins. Sugar metabolism resulting in the generation of ATP and reducing agent (NADPH), as well as the expression of some key enzymes (dehydrogenases) were also upregulated by DBP treatment. Moreover, a diverse bacterial community appears to utilize sugar, suggesting that there are widespread effects of DBP contamination on soil microbial ecosystems. The results of this study provide a theoretical basis for investigating the toxicological effects of DBP on microbes in black soil.
اظهر المزيد [+] اقل [-]Impact of mariculture-derived microplastics on bacterial biofilm formation and their potential threat to mariculture: A case in situ study on the Sungo Bay, China
2020
Sun, Xuemei | Chen, Bijuan | Xia, Bin | Li, Qiufen | Zhu, Lin | Zhao, Xinguo | Gao, Yaping | Qu, Keming
Microplastics (MPs) pollution in the marine environment has attracted considerable global attention. However, the colonization of microorganisms on mariculture-derived MPs and their effects on mariculture remain poorly understood. In this study, the MPs (fishing nets, foams and floats) and a natural substrate, within size ranges (1–4 mm), were then incubated for 21 days in Sungo Bay (China), and the composition and diversity of bacterial communities attached on all substrates were investigated. Results showed that bacterial communities on MPs mainly originated from their surrounding seawater and sediment, with an average contribution on total MPs adherent population of 47.91% and 37.33%, respectively. Principle coordinate analysis showed that community similarity between MPs and surrounding seawater decreased with exposure time. In addition, lower average bacterial community diversity and higher relative abundances of bacteria from the genera Vibrio, Pseudoalteromonas and Alteromonas on MPs than those in their surrounding seawater and sediments indicated that MPs might enrich potential pathogens and bacteria related with carbohydrate metabolism. They are responsible for the significant differences in KEGG Orthology pathways (infectious disease and carbohydrate metabolism) between MPs and seawater. The KO pathway (Infectious Diseases) associated with MPs was also significantly higher than those with feathers in the nearshore area. MPs might be vectors for enrichment of potentially pathogenic Vibrio, and enhance the ecological risk of MPs to mariculture industry.
اظهر المزيد [+] اقل [-]Heat shock pretreatment induced cadmium resistance in the nematode Caenorhabditis elegans is depend on transcription factors DAF-16 and HSF-1
2020
Wang, Shunchang | You, Mu | Wang, Chengrun | Zhang, Yuecheng | Fan, Caiqi | Yan, Shoubao
Cadmium (Cd) exposure poses a serious environmental problem due to the metal’s bioaccumulation and difficult to eliminate from body. Understanding the mechanisms of Cd detoxification and resistance can provide insights into methods to protect against the damaging effects of the heavy metal. In the present study, we found that heat shock (HS) pretreatment increased Cd resistance of the nematode Caenorhabditis elegans by reducing the bagging phenotype and protecting the integrity of the intestinal barrier. HS pretreatment increased the expression of heat shock protein-16.2 (HSP-16.2) prior to Cd exposure, and HS-induced Cd resistance was absent in worms with hsp-16.2 loss-of-function mutation. Worm strain with daf-2(e1370) mutation presented enhanced HS-induced Cd resistance, which was eliminated in worm strains of daf-16(mu86) and hsf-1(sy441). HS pretreatment increased DAF-16 nuclear localization and HSF-1 granule formation prior to Cd exposure. DAF-16 and HSF-1 was essential in reducing bagging formation and protecting the integrity of intestinal barrier after HS pretreatment. In conclusion, the present study demonstrated that HS-induced Cd resistance in C. elegans is regulated by the DAF-16/FOXO and HSF-1 pathways through regulation of HSP-16.2 expression.
اظهر المزيد [+] اقل [-]Facile construction of highly reactive and stable defective iron-based metal organic frameworks for efficient degradation of Tetrabromobisphenol A via persulfate activation
2020
Huang, Mei | Wang, Yan | Wan, Jinquan | Ma, Yongwen | Chi, Haiyuan | Xu, Yanyan | Qiu, Shuying
Achieving large pore size, high catalytic performance with stable structure is critical for metal–organic frameworks (MOFs) to have more hopeful prospects in catalytic applications. Herein, we had reported a method to synthesize highly reactive yet stable defective iron-based Metal organic frameworks by using different monocarboxylic acids with varying lengths as a modulator. The physical−chemical characterization illustrating that modulators could improve the crystallinity, enlarge pore size and enhance catalytic performance and octanoic acid (OA) was screened to be the suitable choice. The catalytic performance of catalysts was detected through persulfate (PS) activation for degrading Tetrabromobisphenol A (TBBPA). The study demonstrated that the highest degradation efficiency for 0.018 mmol L−1 TBBPA was that 97.79% in the conditions of the 1.0 g L−1 Fe(BDC)(DMF,F)-OA-30 dosage and TBBPA:PS = 200:1. In addition, there was observed that no obvious change of the crystal structure, little the leachable iron concentration in the solutions and no significant loss of catalytic activities of Fe(BDC)(DMF,F)-OA-30 after 5th cycles. The iron valence state of Fe(BDC)(DMF,F)-OA-30 before and after degradation and electrochemical properties reveal that the partial substitution of organic ligands by octanoic acid, when removing OA and forming defects by heat and vacuum treatment to generate coordinatively unsaturated metal sites and accelerate the original transmission of electronic, leading to enhance the activity of persulfate activation for efficient removal TBBPA.
اظهر المزيد [+] اقل [-]Potentially toxic elements in toys and children’s jewelry: A critical review of recent advances in legislation and in scientific research
2020
Guney, Mert | Kismelyeva, Symbat | Akimzhanova, Zhanel | Beisova, Kamila
Contamination by potentially toxic elements (PTEs) in children’s toys and jewelry is an ongoing problem where PTEs can become bioavailable especially via oral pathway (ingestion as a whole or of parts, and mouthing) and may cause adverse health effects for children. In the present review, legislation updates from the last decade in the United States (U.S.), Canada, and the European Union (E.U.) on PTEs in toys and jewelry are presented. Then, a literature review mostly covering the last decade on the total concentration, bioavailability, children’s exposure, and bioaccessibility of PTEs in toys and jewelry is provided. The U.S. and Canadian legislations mainly focus on lead (Pb) and cadmium (Cd) total/soluble concentration limits to prevent exposure and have received several updates within the last decade, extending particularly the covered span of children’s products. It seems that the introduction, subsequent enforcement, and update of regulations in developed countries have shifted the problem towards developing countries. In terms of categories, metallic toys and children’s jewelry still have the most severe PTE contamination and the presence of Pb and Cd in these articles is an ongoing issue. Some studies suggest that color can be used as an indicator for the potential presence of PTEs (linked to chemicals such as lead chromate, cadmium sulfide) but the evidence is not always clear. Another concern is vintage/second-hand toys and jewelry as those items might have been produced before the legislation was present. As total and bioaccessible concentrations of PTEs in toys and jewelry do not always correlate, approaches considering bioaccessibility (e.g. of the E.U.) are more scientifically appropriate and help with better estimation of risk from exposure. Studies on toy and jewelry contamination using in vitro bioaccessibility techniques has become more common, however, there is still no in vitro test specifically designed and validated for toys and jewelry.
اظهر المزيد [+] اقل [-]Lead contamination from gold mining in Yellowknife Bay (Northwest Territories), reconstructed using stable lead isotopes
2020
Pelletier, Nicolas | Chételat, John | Cousens, Brian | Zhang, Shuangquang | Stepner, Dan | Muir, Derek C.G. | Vermaire, Jesse C.
The contributions of contaminant sources are difficult to resolve in the sediment record using concentration gradients and flux reconstruction alone. In this study, we demonstrate that source partitioning using lead isotopes provide complementary and unique information to concentration gradients to evaluate point-source releases, transport, and recovery of metal mining pollution in the environment. We analyzed eight sediment cores, collected within 24 km of two gold mines, for Pb stable isotopes, Pb concentration, and sediment chronology. Stable Pb isotope ratios (²⁰⁶Pb/²⁰⁷Pb, ²⁰⁸Pb/²⁰⁴Pb) of mining ore were different from those of background (pre-disturbance) sediment, allowing the use of a quantitative mixing model. As previously reported for some Arctic lakes, Pb isotope ratios indicated negligible aerosol inputs to sediment from regional or long-range pollution sources, possibly related to low annual precipitation. Maximum recorded Pb flux at each site reached up to 63 mg m⁻² yr⁻¹ in the period corresponding to early years of mining when pollution mitigation measures were at a minimum (1950s–1960s). The maximum contribution of mining-derived Pb to these fluxes declined with distance from the mines from 92 ± 8% to 8 ± 4% at the farthest site. Mining-derived Pb was still present at the sediment surface within 9 km of Giant Mine more than ten years after mine closure (5–26 km, 95% confidence interval) and model estimates suggest it could be present for another ∼50–100 years. These results highlight the persistence of Pb pollution in freshwater sediment and the usefulness of Pb stable isotopes to quantify spatial and temporal trends of contamination from mining pollution, particularly as concentrations approach background.
اظهر المزيد [+] اقل [-]Three organophosphate flame retardants (OPFRs) reduce sperm quality in Chinese rare minnows (Gobiocypris rarus)
2020
Chen, Rui | Hong, Xiangsheng | Yan, Saihong | Zha, Jinmiao
Organophosphate flame retardants (OPFRs) are widespread in the aquatic environment, but the effects of these chemicals on reproductive toxicity are far from clear. In this study, sperm quality in adult male Chinese rare minnows after exposure to tris-(2-butoxyethyl) phosphate (TBOEP), tris-(1,3-dichloro-2-propyl) phosphate (TDCIPP), and triphenyl phosphate (TPHP) was investigated. No obvious change in sperm concentration and vitality was observed after treatments, whereas significant changes in sperm velocity and morphology were found following all treatments (P < 0.05). Moreover, OPFR exposure significantly increased the apoptosis ratios in testis cells. Analysis of the transcriptomic data revealed that Na⁺/K⁺ ATPase (NKA) related genes were significantly downregulated, and the NKA enzyme activities after all treatments were significantly inhibited (P < 0.05). However, no obvious change in hormone levels in the groups exposed to TBOEP and TDCIPP was observed. These findings indicate that the OPFR-induced reduction of sperm quality might be due to the effects of OPFRs on NKA enzyme instead of changes in hormone levels.
اظهر المزيد [+] اقل [-]Exposure to etoxazole induces mitochondria-mediated apoptosis in porcine trophectoderm and uterine luminal epithelial cells
2020
Park, Wonhyoung | Lim, Whasun | Park, Sunwoo | Whang, Kwang-Youn | Song, Gwonhwa
Etoxazole is an organofluorine insecticide widely used in agriculture. Exposure to insecticides is a serious environmental problem owing to their cytotoxic effects in humans and animals. Reproductive toxicity of various organofluorine insecticides have been shown in previous studies. However, few studies have evaluated the toxicity of etoxazole in mammals. We aimed to examine the toxic effects of etoxazole in porcine trophectoderm (pTr) and uterine luminal epithelial (pLE) cells. To estimate the effects of etoxazole, we conducted assays after treatment with multiple concentration of etoxazole (0, 2, 4, 6 and 9 μM) to pTr and pLE cells for 0–72 h. Etoxazole decreased the cell proliferation, viability, and migration of pTr and pLE cells. Further, etoxazole induced apoptosis via cell cycle arrest and disruption of mitochondrial membrane potential. We also found that pro-apoptotic proteins and endoplasmic reticulum (ER) stress-response proteins were activated in response to etoxazole. Finally, we observed that etoxazole altered the PI3K/AKT and MAPK signaling pathways and the mRNA expression of genes associated with implantation. Collectively, these results suggest that etoxazole disrupts normal cellular physiology and might cause early implantation failure.
اظهر المزيد [+] اقل [-]Formation and mobilization of methylmercury across natural and experimental sulfur deposition gradients
2020
Åkerblom, Staffan | Nilsson, Mats B. | Skyllberg, Ulf | Bjørn, Erik | Jonsson, Sofi | Ranneby, Bo | Bishop, Kevin
We investigated the influence of sulfate (SO₄²⁻) deposition and concentrations on the net formation and solubility of methylmercury (MeHg) in peat soils. We used data from a natural sulfate deposition gradient running 300 km across southern Sweden to test the hypothesis posed by results from an experimental field study in northern Sweden: that increased loading of SO₄²⁻ both increases net MeHg formation and redistributes methylmercury (MeHg) from the peat soil to its porewater. Sulfur concentrations in peat soils correlated positively with MeHg concentrations in peat porewater, along the deposition gradient similar to the response to added SO₄²⁻ in the experimental field study. The combined results from the experimental field study and deposition gradient accentuate the multiple, distinct and interacting roles of SO₄²⁻ deposition in the formation and redistribution of MeHg in the environment.
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