Lake eutrophication has become a serious environmental problem in China. Manipulations covering more elements of trophic pyramid are methods for lakes to obtain clear water state and should be studied in detail. In the present study, Meishan Dongpo Lake was divided into two parts, and a combined biomanipulation project was conducted in one part (RLake), and the other part was used as a control (CLake). Species of submerged-plant, fish, macrobenthos, and zooplankton were screened and a certain number of them were added to adjust the eco-chain in RLake. After restoration, the coverage of submerged macrophytes reached >85%; zooplankton greater than 0.6 mm in size increased in number, and the ratio of zooplankton biomass to chlorophyll-a (Chl-a) concentration increased. The dominant fish species changed, and disturbance of the sediment was reduced. The average density of mollusks in RLake was 111.5 ± 19.8 ind m⁻², which was much higher than that in CLake (36.7 ± 2.1 ind m⁻²). Water quality and clarity were substantially improved, and nutrient concentrations, particularly total phosphorus, total nitrogen, and Chl-a were significantly reduced. The aquatic community parameters were negatively correlated with the nutrient parameters and Chl-a. The ecological restoration have adjusted the aquatic ecosystem in RLake, and many positive feedback effects among the aquatic communities made them remove internal nutrients and Chl-a more efficiently.

Total concentrations of hydrophobic organic contaminants (HOCs) in sediment present a poor quality assessment parameter for aquatic organism exposure and environmental risk because they do not reflect contaminant bioavailability. The bioavailability issue of HOCs in sediments can be addressed by application of multi-ratio equilibrium passive sampling (EPS). In this study, riverbed sediment samples were collected during the Joint Danube Survey at 9 locations along the Danube River in 2013. Samples were ex-situ equilibrated with silicone passive samplers. Desorption isotherms were constructed, yielding two endpoints: pore water (CW:₀) and accessible (CAS:₀) concentration of polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs), polycyclic aromatic hydrocarbons (PAHs) and polybrominated diphenyl ethers in sediment. CW:₀ concentrations of DDT and its breakdown products exhibited elevated levels in the low Danube, with the maximum in the river delta. Other investigated HOCs did not show any clear spatial trends along the river, and only a moderate CW:₀ variability. CAS:₀ in sediment ranged from 10 to 90% of the total concentration in sediment. CW:₀ was compared with freely dissolved concentration in the overlaying surface water, measured likewise by passive sampling. The comparison indicated potential compound release from sediment to the water phase for PAHs with less than four aromatic rings, and for remaining HOCs either equilibrium between sediment and water, or potential compound deposition in sediment. Sorption partition coefficients of HOC to organic carbon correlated well with octanol-water partition coefficients (KOW), showing stronger sorption of PAHs to sediment than that of PCBs and OCPs having equal logKOW. Comparison of CW:₀ values with European environmental quality standards indicated potential exceedance for hexachlorobenzene, fluoranthene and benzo[a]pyrene at several sites. The study demonstrates the utility of passive sampling as an innovative approach for risk-oriented monitoring of HOCs in river catchments.

The pressure on natural water resources associated with increasing water scarcity highlights the value of using reclaimed water through the development of efficient and environmentally friendly treatment technologies. In this work, the use of magnetic nanoparticles in photo-Fenton catalysis for water disinfection was considered to inactivate natural enteric bacteria present in municipal wastewater effluents under white light and neutral pH. The most recommended ranges were evaluated in key variables such as the loading and composition of nanoparticles (NPs), hydrogen peroxide (H₂O₂) concentration, the light source (UV and visible) and treatment time were evaluated in wastewater disinfection expressed in terms of total coliforms and Escherichia coli colony forming units (CFU). The magnetic separation of NPs allowed the disinfection process to be carried out in different cycles, facilitating the recovery of the nanocatalyst and avoiding its discharge with the treated effluent.

The 2011 spill at platforms B and C of the Penglai 19-3 oil field in the Bohai Sea has been the worst oil spill accident in China. To assess long-term effects, a comprehensive monitoring program of chemical and biological variables (within a 2.2 km radius of the spill site) was conducted five years after the spill. Comparison of nutrient, Chl-a and oil concentrations in seawater, TOC, PAHs, heavy metals concentrations within the sediments, and the abundance and biomass of macrobenthic organisms to values obtained before and after the oil spill in previous studies indicate habitat recovery has occurred within the Bohai Sea following the episodic oil release. Observed elevated oil concentration in the water column and higher concentrations of two heavy metals, five PAHs, TOC, TOC/TN and lower values of δ¹³C, together with a reduction in macrobenthic biomass in near-field samples, suggest the influence of contaminants from chronic releases of oil and operational waste discharges within the vicinity of the oil platforms.

Glyphosate-based herbicides, the most extensively used herbicides in the world, are available in an enormous number of commercial formulations with varying additives and adjuvants. Here, we study the effects of one such formulation, Credit41, in two genetically diverse yeast strains. A quantitative trait loci (QTL) analysis between a sensitive laboratory strain and a resistant strain linked mitochondrial function to Credit41 resistance. Two genes encoding mitochondrial proteins identified through the QTL analysis were HFA1, a gene that encodes a mitochondrial acetyl CoA carboxylase, and AAC3, which encodes a mitochondrial inner membrane ATP/ADP translocator. Further analysis of previously studied whole-genome sequencing data showed that, although each strain uses varying routes to attain glyphosate resistance, most strains have duplications of mitochondrial genes. One of the most well-studied functions of the mitochondria is the assembly of Fe–S clusters. In the current study, the expression of iron transporters in the transcriptome increased in cells resistant to Credit41. The levels of iron within the cell also increased in cells exposed to Credit41 but not pure glyphosate. Hence, the additives in glyphosate-based herbicides have a significant contribution to the negative effects of these commercial formulations on biological systems.

A dissolved air flotation (DAF) system is one of the water treatment processes that purifies contaminants through a buoyancy effect by attaching the moiety of micro-bubbles on their free surface. Since the DAF system was first used in the drinking water treatment in the 1960s, it has been recognized as an effective treatment for the water purification process. Most previous works laid great emphasis on the internal flow behaviors of fluid to improve the purification efficiency of the DAF system. Nevertheless, the practical implementation with a pilot plant indeed revealed some technical incompleteness for the DAF system. To circumvent for the technical incompleteness, numerical simulation based on computational fluid dynamics (CFD) has been carried out to understand the in-depth knowledge on internal flow phenomena in the DAF system. However, the standard k-ε turbulence model has been conventionally used in the most studies without any proper consideration process. Accordingly, the objectives of this study were to investigate the major effects on the internal flow behaviors for an efficient numerical simulation of DAF when a different turbulence model and micro-bubble parameters are used. As a result, the present study found that the standard k-ε model would be not proper for the internal flow simulation of the DAF process and a careful consideration would be required for a more accurate prediction. In addition, the present study examined a desirable internal flow pattern with various operating conditions of the micro-bubble. Consequently, the main findings of this study are expected to provide realistic information to related researchers for designing the DAF system with the optimal operating parameters.

Despite the mercury (Hg) control measures adopted by the international community, Hg still poses a significant risk to ecosystem and human health. This is primarily due to the ability of atmospheric Hg to travel intercontinentally and contaminating terrestrial and aquatic environments far from its natural and anthropogenic point sources. The issue of Hg pollution is further complicated by its unique physicochemical characteristics, most noticeably its multiple chemical forms that vary in their toxicity and environmental mobility. This meant that most of the risk evaluation protocols developed for other metal(loid)s are not suitable for Hg. Soil is a major reservoir of Hg and a key player in its global cycle. To fully assess the risks of soil Hg it is essential to estimate its bioavailability and/or availability which are closely linked to its toxicity. However, the accurate determination of the (bio)-available pools of Hg in soils is problematic, because the terms ‘bioavailable’ and ‘available’ are ill-defined. In particular, the term ‘bioavailable pool’, representing the fraction of Hg that is accessible to living organisms, has been consistently misused by interchanging with other intrinsically different terms e.g. mobile, labile, reactive and soluble pools. A wide array of physical, chemical, biological and isotopic exchange methods were developed to estimate the (bio)-available pools of Hg in soil in an attempt to offer a plausible assessment of its risks. Unfortunately, many of these methods do not mirror the (bio)-available pools of soil Hg and suffer from technical drawbacks. In this review, we discuss advantages and disadvantages of methods that are currently applied to quantify the (bio)-availability of Hg in soils. We recommended the most feasible methods and give suggestions how to improve the determination of (bio)-available Hg in soils.

The carbon dots (C-dots) mediated Sn₂Ta₂O₇/SnO₂ heterostructures with spongy structure were successfully assembled by simple hydrothermal route. The photocatalytic removal efficiency of amoxicillin (AMX, 20 mg L⁻¹) over C-dots/Sn₂Ta₂O₇/SnO₂ was estimated to reach up 88.3% within 120 min simulated solar light irradiating. Meanwhile, the HPLC-MS/MS analysis and density functional theory (DFT) computation were examined to clarify the photo-degradation pathway of AMX. The mechanism investigation proposed that with the modification of C-dots, the photocatalysts improves the utilization of solar energy by harvesting the long wavelength solar light due to their unique up-converted photoluminescence (UCPL). In addition, the porous spongy structure and plenty of tiny C-dots promote the ability of adsorption by enlarged specific surface area. Furthermore, the C-dots mediated Z-type heterojunction of Sn₂Ta₂O₇/SnO₂ facilitates the efficient separation and transfer of photo-induced carriers. Our work affords a promising approach for the design of the high-efficient photocatalysts to remedy poisonous antibiotics in aqueous environment.

A tropical soil Actinomycete, Gordonia amicalis HS-11, has been previously demonstrated to degrade unsaturated and saturated hydrocarbons (squalene and n-hexadecane, respectively) in an effective manner. In present study, G. amicalis HS-11 degraded 92.85 ± 3.42% of the provided diesel oil [1% (v/v)] after 16 days of aerobic incubation. The effect of different culture conditions such as carbon source, nitrogen source, pH, temperature, and aeration on degradation was studied. During degradation, this Actinomycete synthesized surface active compounds (SACs) in an extracellular manner that brought about a reduction in surface tension from 69 ± 2.1 to 30 ± 1.1 mN m⁻¹ after 16 days. The morphology of cells grown on diesel was monitored by using a Field Emission Scanning Electron Microscope. Diesel-grown cells were longer and clumped with smooth surfaces, possibly due to the secretion of SACs. The interaction between the cells and diesel oil was studied by Confocal Laser Scanning Microscope. Some cells were adherent on small diesel droplets and others were present in the non-attached form thus confirming the emulsification ability of this organism. The fatty acid profiles of the organism grown on diesel oil for 48 h were different from those on Luria Bertani Broth. The genotoxicity and cytotoxicity of diesel oil before and after degradation were determined. Cytogenetic parameters such as mitotic index (MI); mitosis distribution and chromosomal aberration (type and frequency) were assessed. Oxidative stress was evaluated by measuring levels of catalase, superoxide dismutase and concentration of malondialdehyde. On the basis of these studies it was deduced that the degradation metabolites were relatively non-toxic.

PFASs are highly persistent in both natural and living environment, and pose a significant risk for wildlife and human beings. The present study was carried out to determine the inhibitory behaviours of fourteen PFASs on metabolic activity of two major isoforms of carboxylesterases (CES). The probe substrates 2-(2-benzoyl-3-methoxyphenyl) benzothiazole (BMBT) for CES1 and fluorescein diacetate (FD) for CES2 were utilized to determine the inhibitory potentials of PFASs on CES in vitro. The results demonstrated that perfluorododecanoic acid (PFDoA), perfluorotetradecanoic acid (PFTA) and perfluorooctadecanoic acid (PFOcDA) strongly inhibited CES1 and CES2. The half inhibition concentration (IC₅₀) value of PFDoA, PFTA and PFOcDA for CES1 inhibition was 10.6 μM, 13.4 μM and 12.6 μM, respectively. The IC₅₀ for the inhibition of PFDoA, PFTA and PFOcDA towards CES2 were calculated to be 9.56 μM, 17.2 μM and 8.73 μM, respectively. PFDoA, PFTA and PFOcDA exhibited noncompetitive inhibition towards both CES1 and CES2. The inhibition kinetics parameters (Kᵢ) were 27.7 μM, 26.9 μM, 11.9 μM, 4.04 μM, 29.1 μM, 27.4 μM for PFDoA-CES1, PFTA-CES1, PFOcDA-CES1, PFDoA-CES2, PFTA-CES2, PFOcDA-CES2, respectively. In vitro-in vivo extrapolation (IVIVE) predicted that when the plasma concentrations of PFDoA, PFTA and PFOcDA were greater than 2.77 μM, 2.69 μM and 1.19 μM, respectively, it might interfere with the metabolic reaction catalyzed by CES1 in vivo; when the plasma concentrations of PFDoA, PFTA and PFOcDA were greater than 0.40 μM, 2.91 μM, 2.74 μM, it might interfere with the metabolic reaction catalyzed by CES2 in vivo. Molecular docking was used to explore the interactions between PFASs and CES. In conclusion, PFASs were found to cause inhibitory effects on CES in vitro, and this finding would provide an important experimental basis for further in vivo testing of PFASs focused on CES inhibition endpoints.