Salinity is a limiting factor in the growth of plants in coastal wetlands. The interaction of halophytes with salt-tolerant endophytes has been one of the major concerns in this area. However, the mechanism by which endophytes promote halophyte growth remains unclear. The growth and physiological responses of Suaeda salsa inoculated with endophytic bacteria (Sphingomonas prati and Sphingomonas zeicaulis) at 0 ‰ and 20 ‰ NaCl were studied. The results showed that Sphingomonas zeicaulis had stronger positive effects on the growth of Suaeda salsa under 0 ‰ NaCl, and Sphingomonas prati performed better under 20 ‰ NaCl. Sphingomonas prati inoculation increased the mean height, root length, fresh weight and dry weight by 45.43%, 9.91%, 82.00% and 102.25%, respectively, compared with the uninoculated treatment at 20 ‰ NaCl. Sphingomonas prati inoculation decreased MDA content by 23.78%, while the soluble sugar and soluble protein contents increased by 15.08% and 12.57%, respectively, compared to the control, at 20 ‰ NaCl. Increases in SOD and CAT in the Sphingomonas prati inoculation were 1.03 and 1.47-fold greater, respectively, than in the Sphingomonas zeicaulis inoculation, under 20 ‰ NaCl. Moreover, Sphingomonas prati and Sphingomonas zeicaulis had antagonistic interactions in Suaeda salsa according to the results of the “interaction equation” (most G values were negative). PCA, clustering analysis and the PLS model revealed two mechanisms for regulating plant salt tolerance by which Sphingomonas prati enhanced Suaeda salsa growth: (1) Sphingomonas prati improved intracellular osmotic metabolism and (2) Sphingomonas prati promoted the production of CAT in the antioxidant enzyme system and retained permeability. This study provides new insight into the comprehensive understanding and evaluation of endophytic bacteria as biological inoculants in plants under salt stress.

Co-selection of antibiotic resistance genes (ARGs) by heavy metals might facilitate the spread of ARGs in the environments. Cadmium contamination is ubiquitous, while, it remains unknown the extent to which cadmium (Cd²⁺) impact plasmid-mediated transfer of ARGs in aquatic bacterial communities. In the present study, we found that Cd²⁺ amendment at sub-inhibitory concentration significantly increased conjugation frequency of RP4 plasmid from Pseudomonas putida KT2442 to a fresh water microbial community by liquid mating method. Cd²⁺ treatment (1–100 mg/L) significantly increased the cell membrane permeability and antioxidant activities of conjugation mixtures. Amendments of 10 and 100 mg/L Cd²⁺ significantly enhanced the mRNA expression levels of mating pair formation gene (trbBp) and the DNA transfer and replication gene (trfAp) due to the repression of regulatory genes (korA, korB and trbA). Phylogenetic analysis of transconjugants indicated that Proteobacteria was the dominant recipients and high concentration of Cd²⁺ treatment resulted in expanded recipient taxa. This study suggested that sub-inhibitory Cd²⁺ contamination would facilitate plasmid conjugation and contributed to the maintenance and spread of plasmid associated ARGs, and highlighted the urgent need for effective remediation of Cd²⁺ in aquatic environments.

The presence of pharmaceuticals in the aquatic environment, both in marine and freshwater reservoirs, is a major concern of global environmental protection. Among the drugs that are most commonly used, NSAIDs tend to dominate. Currently, being aware of the problem caused by drug contamination, it is extremely important to evaluate the scale and the full spectrum of its consequences, from short-term to long-term effects. The influence on non-target aquatic animals can take place at many levels, and the effects can be seen both in behaviour and physiology, but also in genetic alterations or reproduction disorders, affecting the development of entire populations. This review summarises all the advances made to estimate the impact of NSAIDs on aquatic animals. Multicellular animals from all trophic levels, inhabiting both inland waters, seas and oceans, have been considered. Particular attention has been paid to chronic studies, conducted at low, environmentally-relevant concentrations, to estimate the real effects of the present pollution. The number of such studies has indeed increased in recent years, allowing for a better insight into the possible consequences of pharmaceutical pollution. It should be stressed, however, that our knowledge is still limited to a few model species, while there are many groups of organisms completely unexplored regarding the effects of drugs. Therefore, the main aim of this paper was to summarise the current state of knowledge on the toxicity of NSAIDs in aquatic animals, also identifying important gaps and major issues requiring further analysis.

Polychlorinated biphenyls (PCBs) may transfer into the neonates through the placental transfer and via breastfeeding after the delivery, thus might be harmful to the infant. Sixty colostrum samples in the Yangtze River Region were collected to investigate the concentration, distribution pattern, and enantiomer characteristic of the PCB exposure. Among all samples, over 90% of pollutants were tetra-to hepta-chlorinated PCBs. The sum concentration of the PCB was 512 (IQR: 322–856) ng g⁻¹ lipid weight. Enantiomer fraction (EF) of PCB 95 and PCB 149 was found lower than the racemic value, while EFs of PCB 45 and PCB 136 were found higher and near-racemic state, respectively. The concentration pattern and enantiomeric properties of the PCBs indicated that the mothers from Mianyang had a recent exposure to PCBs. Among all samples, similar exposure and metabolic pathways of the PCB congeners were observed. PCB exposure showed no significant correlation with the birth outcome of the infants, but 43.3% of the infants have potential health risks via breastfeeding.

Chinese children have been exposed to high level of lead due to polluted air, dust, contaminated foods and water, etc. In this research, we investigated published blood lead levels (BLLs) reflecting 1,057,832 Chinese children aged at 0–12 and teenagers aged at 13–18 in the past 30 years (1991–2020). The data mining and estimation were performed innovatively by Monte Carlo simulation to remedy the skewed distribution-induced bias. The temporal trend of Chinese children’s BLLs showed an obvious decrease in the past decades from 88.74 μg/L (Geometric SD = 4.09) during 1991–1995 to 27.32 μg/L (Geometric SD = 4.18) during 2016–2020. This study also indicated that children’s BLLs of Yunnan, Guizhou, Shanxi were at relatively high levels and most provinces showed a downward trend. Chinese boys aged at 1–18 years old had higher BLLs (GM: 44.03 μg/L) compared to girls (GM: 41.32 μg/L) (p < 0.001). At different age groups, Chinese children’s BLLs were 42.04 μg/L (1–3 years old), 52.88 μg/L (4–6 years old), 50.49 μg/L (7 and above years old), respectively. Although the BLLs of Chinese children exhibited a continuous declined trend in the past 30 years, it was still higher than that in developed countries, which indicated that more efforts are needed in children’s BLLs control.

Environmental exposures are one of the greatest threats to human health, yet we lack tools to answer simple questions about our exposures: what are our personal exposure profiles and how do they change overtime (external exposome), how toxic are these chemicals, and what are the sources of these exposures? To capture variation in personal exposures to airborne chemicals in the gas and particulate phases and identify exposures which pose the greatest health risk, wearable exposure monitors can be deployed. In this study, we deployed passive air sampler wristbands with 84 healthy participants (aged 60–69 years) as part of the Biomarkers for Air Pollutants Exposure (China BAPE) study. Participants wore the wristband samplers for 3 days each month for five consecutive months. Passive samplers were analyzed using a novel gas chromatography high resolution mass spectrometry data-processing workflow to overcome the bottleneck of processing large datasets and improve confidence in the resulting identified features. The toxicity of chemicals observed frequently in personal exposures were predicted to identify exposures of potential concern via inhalation route or other routes of airborne contaminant exposure. Three exposures were highlighted based on elevated toxicity: dichlorvos from insecticides (mosquito/malaria control), naphthalene partly from mothballs, and 183 polyaromatic hydrocarbons from multiple sources. Other exposures explored in this study are linked to diet and personal care products, cigarette smoke, sunscreen, and antimicrobial soaps. We highlight the potential for this workflow employing wearable passive samplers for prioritizing chemicals of concern at both the community and individual level, and characterizing sources of exposures for follow up interventions.

The current study for the first time demonstrates the interference of a free-living, N₂-fixing, and nanoparticle (NP) tolerant Azotobacter salinestris strain ASM recovered from metal-polluted soil with tomato plant-metal oxide NPs (ZnO, CuO, Al₂O₃, and TiO₂) interactions in a sandy clay loam soil system with bulk materials as control. Tomato plants were grown till full maturity in soils amended with 20–2000 mg kg⁻¹ of each metal-oxide NP with and without seed biopriming and root-inoculation of A. salinestris. A. salinestris was found metabolically active, producing considerably high amounts of bioactive indole-3-acetic-acid, morphologically unaffected, and with low alteration of cell membrane permeability under 125–1500 μgml⁻¹ of NPs. However, ZnO-NPs slightly alter bacterial membrane permeability. Besides, A. salinestris secreted significantly higher amounts of extracellular polymeric substance (EPS) even under NP exposure, which could entrap the NPs and form metal-EPS complex as revealed and quantified by SEM-EDX. NPs were also found adsorbed on bacterial biomass. EPS stabilized the NPs and provided negative zeta potential to NPs. Following soil application, A. salinestris improved the plant performance and augmented the yield of tomato fruits and lycopene content even in NPs stressed soils. Interestingly, A. salinestris inoculation enhanced photosynthetic pigment formation, flower attributes, plant and fruit biomass, and reduced proline level. Bacterial inoculation also reduced the NP’s uptake and accumulation significantly in vegetative organs and fruits. The organ wise order of NP’s internalization was roots > shoots > fruits. Conclusively, A. salinestris inoculation could be an alternative to increase the production of tomato in metal-oxide NPs contaminated soils.

Lake water pollution has caused many serious ecological issues globally. An emerging public concern over water quality deterioration in lakes has heightened the need to evaluate the water quality of lakes at long-term scales, particularly for those with high hydrological alterations. This study combines the Mann–Kendall (M–K) test and self-organising map (SOM) to characterise and evaluate water quality trends in Dongting Lake, China, from 1991 to 2018, before and after the inauguration of the Three Gorges Dam (TGD). Herein, six water quality parameters were selected, namely pH, permanganate index (CODMₙ), ammonia nitrogen (NH₃–N), total nitrogen (TN), total phosphorus (TP), and the five-day biochemical oxygen demand (BOD₅). Our results show that the concentrations of TN and BOD₅ increase significantly throughout the study period (|Z| ≥ 1.96). The number of abrupt change points for the six water quality parameters in the post-TGD period was greater than that in the pre-TGD period, which indicates an increased risk of water deterioration in the post-TGD period. The SOM results show that the pH values ranged from 7.64 to 7.85 among the four clusters; besides, the concentrations of the remaining water quality parameters from 1991 to 1997 and 2000 to 2003 were relatively lower, suggesting that the water quality in the pre-TGD period was better. The classification of TN and TP ranged from Level Ⅳ–Ⅴ among the clusters, which did not satisfy the level Ⅲ standard for potable water, thereby posing a higher ecological risk to the Dongting Lake. These results indicate the deterioration of the water quality in Dongting Lake during the post-TGD period under the influences of pollution load and hydrological regulation. Therefore, strict controls on the external nutrient loading and hydrological regulations should be considered for water quality management.

Microplastics receive significant societal and scientific attention due to increasing concerns about their impact on the environment and human health. Marine mammals are considered indicators for marine ecosystem health and many species are of conservation concern due to a multitude of anthropogenic stressors. Marine mammals may be vulnerable to microplastic exposure from the environment, via direct ingestion from sea water, and indirect uptake from their prey. Here we present the first systematic review of literature on microplastics and marine mammals, composing of 30 studies in total. The majority of studies examined the gastrointestinal tracts of beached, bycaught or hunted cetaceans and pinnipeds, and found that microplastics were present in all but one study, and the abundance varied between 0 and 88 particles per animal. Additionally, microplastics in pinniped scats (faeces) were detected in eight out of ten studies, with incidences ranging from 0% of animals to 100%. Our review highlights considerable methodological and reporting deficiencies and differences among papers, making comparisons and extrapolation across studies difficult. We suggest best practices to avoid these issues in future studies. In addition to empirical studies that quantified microplastics in animals and scat, ten studies out of 30 (all focussing on cetaceans) tried to estimate the risk of exposure using two main approaches; i) overlaying microplastic in the environment (water or prey) with cetacean habitat or ii) proposing biological or chemical biomarkers of exposure. We discuss advice and best practices on research into the exposure and impact of microplastics in marine mammals. This work on marine ecosystem health indicator species will provide valuable and comparable information in the future.

The behaviors of typical veterinary antibiotics (oxytetracycline, ciprofloxacin and sulfamethazine) and 75 types of corresponding antibiotic resistant genes (ARGs) in four substrate systems (zeolite, gravel, red brick, and oyster shell) were investigated in this study. The results indicated that during treating synthetic livestock wastewater with individual antibiotic influent concentration of 100 μg/L, the effluent contained oxytetracycline and ciprofloxacin concentrations of 0.7–1.5 μg/L and 1.0–1.9 μg/L, respectively, in the zeolite and red brick systems, which were significantly lower than those of the other substrate systems (4.6–14.5 μg/L). Statistical correlation analyses indicated that the difference regarding oxytetracycline and ciprofloxacin removal among the four substrates was determined by their adsorption capacity which was controlled by the chemisorption mechanism. The average removal efficiency of sulfamethazine in the gravel system (48%) was higher than that of the other substrate systems (34–45%), and biodegradation may alter the sulfamethazine performance because of its co-metabolism process. Although tetG, floR, sul1, and qacEΔ1 were the dominant ARGs in all substrate systems (8.74 × 10⁻²-6.34 × 10⁻¹), there was difference in the total ARG enrichment levels among the four substrates. Oyster shell exhibited the lowest total relative abundance (1.56 × 10⁰) compared to that of the other substrates (1.82 × 10⁰–2.27 × 10⁰), and the ARG total relative abundance exhibited significant negative and positive correlations with the substrate pH and system bacterial diversity (P < 0.05), respectively. In summary, this study indicated that due to the difference of adsorption capacity and residual abundant nutrient in wastewater, the wetland substrate selection can affect the removal efficiency of veterinary antibiotics, and antibiotics may not be the determining factor of ARG enrichment in the substrate system.