خيارات البحث
النتائج 901 - 910 من 4,896
Marine litter from fishery activities in the Western Mediterranean sea: The impact of entanglement on marine animal forests
2019
Consoli, Pierpaolo | Romeo, Teresa | Angiolillo, Michela | Canese, Simonepietro | Esposito, Valentina | Salvati, Eva | Scotti, Gianfranco | Andaloro, Franco | Tunesi, Leonardo
The anthropogenic marine debris, especially abandoned, lost or otherwise discarded Fishing Gear (ALDFG), represents a rising concern, because of its potential harmful impact on the marine animal forests. We carried out 13 km of video recordings, by means of a remotely operated vehicle, from 10 to 210 m depth, in an anthropised area of the Tyrrhenian Sea (Mediterranean Sea). This site, for its high ecological importance and biodiversity value, has been identified for the establishment of a new marine protected area (MPA). The aim of this paper was to assess marine litter abundance and its effects on the benthic fauna. The debris density, in the study area, ranged from 0.24 to 8.01 items/100 m2, with an average of 3.49 (±0.59) items/100 m2. The derelict fishing gear, mainly fishing lines, were the main source of marine debris, contributing 77.9% to the overall litter. The impacts of debris on the benthic fauna were frequently recorded, with 28.5% of the litter entangling corals and impacting habitats of conservation concern. These impacts were exclusively caused by the derelict fishing gear (91.2% by longlines), and the highest percentage (49.1%) of ALDFG causing impacts was observed from 41 to 80 m depth, in the coralligenous biocenosis. The results of the present study will help the fulfilment of “harm” monitoring, as recommended by the Marine Strategy Framework Directive (MSFD) and the UN Environment/MAP Regional Plan on the marine litter management in the Mediterranean Sea. Regarding the actions to reduce the derelict fishing gear, preventive measures are usually preferred instead of the extensive removals based on cost-effectiveness and sustainability. The establishment of a new MPA in the area could be a good solution to reduce ALDFG, resulting in the improvement of the ecological status of this coastal area.
اظهر المزيد [+] اقل [-]Low-dose cadmium exposure acts on rat mesenchymal stem cells via RANKL/OPG and downregulate osteogenic differentiation genes
2019
Lv, Ying-Jian | Wei, Qin-Zhi | Zhang, Yang-Cong | Huang, Rui | Li, Bai-Sheng | Tan, Jian-Bin | Wang, Jing | Ling, Hai-Tuan | Wu, Shi-Xuan | Yang, Xing-Fen
Chronic cadmium (Cd) toxicity is a significant health concern, and the mechanism of long-term low-dose Cd exposure on bone has not been fully elucidated till date. This study aimed to assess the association between rat mesenchymal stem cells (MSCs) and long-term Cd exposure through 38-week intake of CdCl2 at 1 and 2 mg/kg body weight (bw). Increased gene expression of receptor activator of NF-κB ligand (RANKL) and decreased gene expression of osteoprotegerin (OPG) were observed. Fold change of RANKL gene expression (fold change = 1.97) and OPG gene expression (fold change = 1.72) showed statistically significant differences at dose 2 mg/kg bw. Decreased expression of key genes was observed during the early osteogenic differentiation of MSCs. The gene expression of Osterix in 1 mg/kg bw group was decreased by 3.70-fold, and the gene expressions of Osterix, Osteopontin, collagen type I alpha 2 chain (COL1a2) and runt-related transcription factor 2 (RUNX2) in 2 mg/kg bw group were decreased by 1.79, 1.67, 1.45 and 1.35-folds, respectively. Exposure to CdCl2 induced an increase in the renal Cd load, but only an adaptive response was observed, including increased expression of autophagy-related proteins LC3B and Beclin-1, autophagy receptor p62, and heme oxygenase 1 (HO-1), which is an inducible isoform that releases in response to stress. There were no significant changes in the urinary low molecular weight proteins including N-acetyl-b-D-glucosaminidase (NAG), β2-microglobulin and albumin (U-Alb). Urinary calcium (Ca) excretion showed no increase, and no obvious renal histological changes. Taken together, these results indicated that the chronic CdCl2 exposure directly act on MSCs through RANKL/OPG pathway and downregulate the key genes involved in osteogenic differentiation of MSCs. The toxic effect of Cd on bone may occur in parallel to nephrotoxicity.
اظهر المزيد [+] اقل [-]Oiling of the continental shelf and coastal marshes over eight years after the 2010 Deepwater Horizon oil spill
2019
Turner, R Eugene | Rabalais, Nancy N. | Overton, Edward B. | Meyer, Buffy M. | McClenachan, Giovanna | Swenson, Erick M. | Besonen, Mark | Parsons, Michael L. | Zingre, Jeffrey
We measured the temporal and spatial trajectory of oiling from the April, 2010, Deepwater Horizon oil spill in water from Louisiana's continental shelf, the estuarine waters of Barataria Bay, and in coastal marsh sediments. The concentrations of 28 target alkanes and 43 target polycyclic aromatic hydrocarbons were determined in water samples collected on 10 offshore cruises, in 19 water samples collected monthly one km offshore at 13 inshore stations in 2010 and 2013, and in 16–60 surficial marsh sediment samples collected on each of 26 trips. The concentration of total aromatics in offshore waters peaked in late summer, 2010, at 100 times above the May, 2010 values, which were already slightly contaminated. There were no differences in surface or bottom water samples. The concentration of total aromatics declined at a rate of 73% y−1 to 1/1000th of the May 2010 values by summer 2016. The concentrations inside the estuary were proportional to those one km offshore, but were 10–30% lower. The oil concentrations in sediments were initially different at 1 and 10 m distance into the marsh, but became equal after 2 years. Thus, the distinction between oiled and unoiled sites became blurred, if not non-existent then, and oiling had spread over an area wider than was visible initially. The concentrations of oil in sediments were 100–1000 times above the May 2010 values, and dropped to 10 times higher after 8 years, thereafter, demonstrating a long-term contamination by oil or oil residues that will remain for decades. The chemical signature of the oil residues offshore compared to in the marsh reflects the more aerobic offshore conditions and water-soluble tendencies of the dissolved components, whereas the anaerobic marsh sediments will retain the heavier molecular components for a long time, and have a consequential effect on the ecosystems.
اظهر المزيد [+] اقل [-]Clock-Bmal1 mediates MMP9 induction in acrolein-promoted atherosclerosis associated with gut microbiota regulation
2019
Wu, Xiaoyue | Chen, Lijun | Zeb, Falak | Li, Chaofeng | Jiang, Pan | Chen, Aochang | Xu, Chuyue | Haq, Ijaz ul | Feng, Qing
Circadian rhythm is believed to play important roles in atherosclerosis. The gut microbiota is found to be closely related to atherogenesis, and shows compositional and functional circadian oscillation. However, it's still unclarified whether circadian clock and intestinal microbiota are involved in the progression of atherosclerosis induced by environmental pollutant acrolein. Herein, patients with atherosclerosis showed higher MMP9, a promising biomarker for atherosclerosis, and lower Bmal1 and Clock expression in the plasma. Interestingly, acrolein exposure contributed to the increased MMP9, decreased Clock and Bmal1, and activated MAPK pathways in human umbilical vein endothelial cells (HUVECs). We found that knockdown of Clock or Bmal1 lead to upregulation of MMP9 in HUVECs, and that Clock and Bmal1 expression was elevated while MAPK pathways were blocked. Atherosclerotic apolipoproteinE-deficient mice consumed a high-fat diet were used and treated with acrolein (3 mg/kg/day) in the drinking water for 12 weeks. Upregulation of MMP9, and downregulation of Clock and Bmal1 were also observed in plasma of the mice. Besides, acrolein feeding altered gut microbiota composition at a phylum level especially for an increased Firmicutes and a decreased Bacteroidetes. Additionally, gut microbiota showed correlation with atherosclerotic plaque, MMP9 and Bmal1 levels. Therefore, our findings indicated that acrolein increased the expression of MMP9 through MAPK regulating circadian clock, which was associated with gut microbiota regulation in atherosclerosis. Circadian rhythms and gut microbiota might be promising targets in the prevention of cardiovascular disease caused by environmental pollutants.
اظهر المزيد [+] اقل [-]Differential patterns of nitrogen and δ15N in soil and foliar along two urbanized rivers in a subtropical coastal city of southern China
2019
Mgelwa, Abubakari Said | Hu, Ya-Lin | Liu, Jin-Fu | Qiu, Qingyan | Liu, Zheng | Yannick Ngaba, Mbezele Junior
Urbanization usually pollutes the environment leading to alterations in key biogeochemical cycles. Therefore, understanding its effects on forest nitrogen (N) saturation is becoming increasingly important for addressing N pollution challenges in urban ecosystems. In this study, we compared soil (N availability, net N mineralization, net nitrification, and δ¹⁵N) and foliar (N concentrations and δ¹⁵N) variables in upstream, midstream and downstream forest stands of Bailongjiang River (BJR; more urbanized) and Wulongjiang River (WJR; less urbanized), the two branches of the Minjiang River Estuary. Total soil N, ammonium, nitrate, net N mineralization and nitrification rates, as well as soil δ¹⁵N were significantly higher in BJR compared with WJR forest stands. While no substantial difference in foliar N concentrations was noted between rivers, foliar δ¹⁵N was on average more than 2.5 times higher in BJR than WJR forest stands. Across the study area, foliar δ¹⁵N was positively related to soil δ¹⁵N, which also had positive linear relationships with soil nitrate concentrations, net N mineralization and net nitrification rates. Moreover, all variables except foliar δ¹⁵N and ammonium concentrations showed decreasing patterns in the order: upstream > midstream > downstream along the BJR forest stands. Soil ammonium and foliar values (N concentrations and δ¹⁵N) revealed clear patterns along the WJR, with the former increasing and the latter decreasing from the upstream to downstream forest stands. Our findings indicate an increase in urbanization-induced N inputs from the WJR to BJR and that forest stands along the BJR especially at the upstream have higher N availability and are shifting rapidly towards N saturation state. These results emphasize the need for effective N pollution control in urban environments through sustainable urban planning.
اظهر المزيد [+] اقل [-]Intensity dependent disruptive effects of light at night on activation of the HPG axis of tree sparrows (Passer montanus)
2019
Zhang, Xinjie | Yang, Wenyu | Liang, Wei | Wang, Yong | Zhang, Shuping
Artificial light at night (ALAN) has become increasingly recognized as a disruptor of the reproductive endocrine process and behavior of wild birds. However, there is no evidence that ALAN directly disrupt the hypothalamus-pituitary-gonadal (HPG) axis, and no information on the effects of different ALAN intensities on birds. We experimentally tested whether ALAN affects reproductive endocrine activation in the HPG axis of birds, and whether this effect is related to the intensity of ALAN, in wild tree sparrows (Passer montanus). Forty-eight adult female birds were randomly assigned to four groups. They were first exposed to a short light photoperiod (8 h light and 16 h dark per day) for 20 days, then exposed to a long light photoperiod (16 h light and 8 h dark per day) to initiate the reproductive endocrine process. During these two kinds of photoperiod treatments, the four groups of birds were exposed to 0, 85, 150, and 300 lux light in the dark phase (night) respectively. The expression of the reproductive endocrine activation related TSH-β, Dio2 and GnRH-I gene was significantly higher in birds exposed to 85 lux light at night, and significantly lower in birds exposed to 150 and 300 lux, relative to the 0 lux control. The birds exposed to 85 lux had higher peak values of plasma LH and estradiol concentration and reached the peak earlier than birds exposed to 0, 150, or 300 lux did. The lower gene expression of birds exposed to 150 and 300 lux reduced their peak LH and estradiol values, but did not delay the timing of these peaks compared to the control group. These results reveal that low intensity ALAN accelerates the activation of the reproductive endocrine process in the HPG axis, whereas high intensity ALAN retards it.
اظهر المزيد [+] اقل [-]Endocrine disrupting chemicals in wild freshwater fishes: Species, tissues, sizes and human health risks
2019
Zhou, Xinyi | Yang, Zhaoguang | Luo, Zhoufei | Li, Haipu | Chen, Guoyao
Increasing attention has been devoted to the adverse effects of endocrine disrupting chemicals (EDCs) on aquatic environments, such as water, sediment and sludge. To date, few studies have investigated the bio-accumulative characteristics of EDCs in different tissues of diverse wild freshwater fish species and their combined impacts on human health. Five EDCs were investigated in the muscle, liver, gill and, especially, gonad of three fish species collected from the Xiangjiang River, southern China. Carnivorous Siniperca Chuatsi or omnivorous Cyprinus Carpio accumulated higher contents of bisphenol A (BPA) and estrone than herbivorous Parabramis Pekinensis in muscle. Furthermore, 4-n-nonylphenol and estrone were found at higher levels and more frequently in the liver, implying that the liver played an important role in basic metabolism for accumulation, biotransformation and excretion of EDCs. Highest concentrations of BPA found in the gonad revealed that the BPA may pose a serious threat to the reproductive system of aquatic organisms. The mean liver/muscle concentration ratios of 4-n-nonylphenol, BPA, estrone and 17α-ethynyl estradiol confirmed the prolonged exposure of the fish to these EDCs. In addition, the relationships between the fish sizes and the EDC concentrations analyzed by Pearson correlation analysis implied that the bioaccumulation of diethylstilbestrol and BPA increased with the growth of Parabramis Pekinensis, and there was a balance between the uptake rate and elimination rate of EDCs in Siniperca Chuatsi and Cyprinus Carpio. Most importantly, the cumulative impacts of combined EDCs on human health by fish consumption were evaluated. The total estradiol equivalent quantity of estrogens was higher than that of phenols. Also, based on the results of the Monte-Carlo simulation, the 95th percentile values of the total estimated daily intakes from consuming the three freshwater fish species from the Xiangjiang River were higher than the acceptable daily intake.
اظهر المزيد [+] اقل [-]Size-dependent adsorption of antibiotics onto nanoparticles in a field-scale wastewater treatment plant
2019
Yu, Kaifeng | Sun, Chi | Zhang, Bo | Hassan, Muhammad | He, Yiliang
This work present aims to evaluate the effect of a conventional wastewater treatment process on the number of nanoparticles, and the role of nanoparticles as a carrier of antibiotics. A set of methods based on asymmetrical flow field flow fractionation coupled with multi-angle light scattering to separate and quantify nanoparticles in real wastewater was established. The characterization of nanoparticles was conducted by transmission electron microscopy, energy dispersive spectrometer, UV–visible spectrophotometer and three-dimensional excitation-emission matrix fluorescence spectroscopy. The adsorption of different sizes of nanoparticles separated from the real wastewater for four targeted antibiotics (sulfadiazine, ofloxacin, tylosin and tetracycline) was studied. The results show that the number of nanoparticles were increased in the wastewater treatment process and the size range between 60 and 80 nm was predominant in wastewater samples. The nanoparticles were mainly composed of O, Si, Al and Ca elements and organic components were in the size range of 0–10 nm. Targeted antibiotics were dominantly adsorbed onto nanoparticles with 60–80 nm size range at each stage. The concentrations of tetracycline adsorbed on nanoparticles were surprisingly increased in the end of the treatment process, while ofloxacin and tylosin had the completely opposite phenomenon to tetracycline. The pH and ionic strength definitely affected the aggregation of nanoparticles and interaction with the antibiotics. It is of great significance to give insights into nanoparticle-antibiotic assemblages for the effective treatment and avoiding the water risks due to nanoparticles’ ubiquitous and their risks of carrying antibiotics.
اظهر المزيد [+] اقل [-]Exposures to chemical contaminants: What can we learn from reproduction and development endpoints in the amphibian toxicology literature?
2019
Slaby, Sylvain | Marin, Matthieu | Marchand, Guillaume | Lemiere, Sébastien
Environmental contamination is one of the major factors or cofactors affecting amphibian populations. Since 2000, the number of studies conducted in laboratory conditions to understand impacts of chemical exposures increased. They aimed to characterize biological effects on amphibians. This review proposes an overview of biological responses reported after exposures to metals, phytopharmaceuticals or emerging organic contaminants and focuses on endpoints relating to reproduction and development. Due to amphibian peculiar features, these periods of their life cycle are especially critical to pollutant exposures.Despite the large range of tested compounds, the same model species are often used as biological models and morphological alterations are the most studied observations. From the results, the laboratory-to-field extrapolation remained uneasy and exposure designs have to be more elaborated to be closer to environmental conditions. Few studies proposed such experimental approaches. Lastly, gametes, embryos and larvae constitute key stages of amphibian life cycle that can be harmed by exposures to freshwater pollutants. Specific efforts have to be intensified on the earliest stages and notably germ cells.
اظهر المزيد [+] اقل [-]Glutathione alleviates the cadmium exposure-caused porcine oocyte meiotic defects via eliminating the excessive ROS
2019
Zhou, Changyin | Zhang, Xue | Chen, Yixuan | Liu, Xiangping | Sun, Yuxin | Xiong, Bo
Heavy metal cadmium (Cd) is a widespread environmental contaminant with a potential toxicity that might adversely influence the health of experimental animals and humans. It has been known that Cd might accumulate in vertebrates for many years and thus leads to the hepatic and renal toxicity. Additionally, Cd concentration in the ovary increases with age and is highly related to the reproductive hazard. However, the underlying mechanisms regarding how Cd affects the female reproductive system especially the oocyte quality have not yet fully defined. Here, we reported that Cd exposure led to the defective nuclear maturation of oocytes via the impairment of cytoskeleton assembly, displaying the aberrant spindle organization, chromosome alignment and actin polymerization. In the meantime, Cd exposure caused the impaired cytoplasmic maturation by showing the disrupted dynamics of mitochondrial integrity and cortical granules, and thereby resulting in the compromised sperm binding ability and fertilization capacity of oocytes. More importantly, we found that glutathione (GSH) supplementation was able to recover the meiotic failure induced by Cd exposure through suppressing the excessive ROS level, DNA damage accumulation and apoptotic incidence. Taken together, our findings demonstrate that Cd exposure has the adverse effects on the oocyte meiotic maturation as well as subsequent fertilization, and provide a potential effective strategy to improve the quality of Cd-exposed oocytes.
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