خيارات البحث
النتائج 911 - 920 من 4,940
Creating a hierarchy of hazard control for urban stormwater management
2019
Ma, Yukun | Deilami, Kaveh | Egodawatta, Prasanna | Liu, An | McGree, James | Goonetilleke, Ashantha
Urban stormwater reuse is becoming increasingly prevalent to overcome the serious urban water scarcity being experienced around the world. Therefore, the adoption of reliable approaches to minimise the human health risk posed by pollutants commonly present in urban stormwater such as heavy metals and polycyclic aromatic hydrocarbons (PAHs) is critical for safe stormwater reuse. This study collected a total of 40 pollutant build-up samples and analysed the concentrations of nine heavy metals and 15 PAH species. Based on pollutant build-up data, pollutant concentrations in stormwater were estimated through modelling. Risk assessment was conducted using an existing model developed by previous studies. The study outcomes confirmed that simply evaluating the individual pollutant concentrations based on guideline threshold values cannot comprehensively estimate the overall human health risk posed by these pollutants. Accordingly, it is recommended that the assessment of the overall human health risk should be based on the pollutant mix present as provided by the models discussed in this paper. The study has also demonstrated the practical application of a robust risk assessment model to derive the hierarchy of hazard control to provide a reliable underpinning to urban stormwater risk management. The outcomes suggest that decentralised hazard control methods such as the provision of custom designed Water Sensitive Urban Design (WSUD) measures can be implemented in priority areas with high risk from stormwater pollution based on the risk assessment undertaken. Distributed hazard control methods can be applied to reduce the generation of primary toxic pollutants, especially chromium (Cr) and heavy PAHs, through elimination and substitution measures. The percentage reduction in traffic volume required to mitigate the human health risk can be quantified through the risk models presented. The study outcomes will contribute to the development of efficient, targeted and reliable stormwater management strategies and to identify viable opportunities for stormwater reuse.
اظهر المزيد [+] اقل [-]Perfluorooctanoic acid exposure induces apoptosis in SMMC-7721 hepatocellular cancer cells
2019
Lv, Da | Gu, Yi | Guo, Ming | Hou, Ping | Li, Yan | Wu, Ronghui
To better understand the toxicological activities of perfluorooctanoic acid (PFOA), we examined the effects of PFOA on apoptosis and its molecular mechanism in SMMC-7721 hepatoma cells. Cell viability was evaluated by MTT assay and apoptosis was determined by flow cytometry. Western blot and quantitative real-time PCR were used to examine the protein and gene expressions of Bax and Bcl-2. Our results showed that PFOA inhibited SMMC-7721 cell growth and induced apoptosis. PFOA treatment increased Bax expression and increased Bcl-2 expression at both gene and protein levels. Our study demonstrated that PFOA had toxic effects on SMMC-7721 cells, such as inhibiting cell proliferation and inducing apoptosis. Furthermore, we showed that PFOA-mediated induction of apoptosis involved inducing Bax and decreasing Bcl-2 expression as a molecular mechanism of its toxicological effects.
اظهر المزيد [+] اقل [-]Transgenerational endocrine disruption: Does elemental pollution affect egg or nestling thyroid hormone levels in a wild songbird?
2019
Ruuskanen, Suvi | Espín, Silvia | Sánchez-Virosta, Pablo | Sarraude, Tom | Hsu, Bin-Yan | Pajunen, Piia | Costa, Rute A. | Eens, Marcel | Hargitai, Rita | Török, János | Eeva, Tapio
Endocrine disrupting chemicals (EDCs) include a wide array of pollutants, such as some metals and other toxic elements, which may cause changes in hormonal homeostasis. In addition to affecting physiology of individuals directly, EDCs may alter the transfer of maternal hormones to offspring, i.e. causing transgenerational endocrine disruption. However, such effects have been rarely studied, especially in wild populations. We studied the associations between environmental elemental pollution (As, Cd, Cu, Ni, Pb) and maternally-derived egg thyroid hormones (THs) as well as nestling THs in great tits (Parus major) using extensive sampling of four pairs of polluted and reference populations across Europe (Finland, Belgium, Hungary, Portugal). Previous studies in these populations showed that breeding success, nestling growth and adult and nestling physiology were altered in polluted zones compared to reference zones. We sampled non-incubated eggs to measure maternally-derived egg THs, measured nestling plasma THs and used nestling faeces for assessing local elemental exposure. We also studied whether the effect of elemental pollution on endocrine traits is dependent on calcium (Ca) availability (faecal Ca as a proxy) as low Ca increases toxicity of some elements. Birds in the polluted zones were exposed to markedly higher levels of toxic elements than in reference zones at the populations in Finland, Belgium and Hungary. In contrast to our predictions, we did not find any associations between overall elemental pollution, or individual element concentrations and egg TH and nestling plasma TH levels. However, we found some indication that the effect of metals (Cd and Cu) on egg THs is dependent on Ca availability. In summary, our results suggest that elemental pollution at the studied populations is unlikely to cause overall TH disruption and affect breeding via altered egg or nestling TH levels with the current elemental pollution loads. Associations with Ca availability should be further studied.
اظهر المزيد [+] اقل [-]Enantiomeric environmental behavior, oxidative stress and toxin release of harmful cyanobacteria Microcystis aeruginosa in response to napropamide and acetochlor
2019
Xie, Jingqian | Zhao, Lu | Liu, Kai | Liu, Weiping
Harmful algal blooms have emerged as a worldwide issue. After concentrations of herbicides entering water, herbicides in water may pose ecological effects on them. The present study investigates the toxicity and environmental behavior of the herbicides, napropamide and acetochlor as enantiomers and as racemates on Microcystis aeruginosa which is the main specie known to produce hepatotoxins. S-napropamide/acetochlor are degraded faster than their corresponding isomer R-napropamide/acetochlor, with the latter more prone to accumulate in algal cells. Moreover, all the enantiomers did not undergo measurable racemization in the medium and algal cells. S-napropamide/acetochlor exhibited much higher toxicity than R-napropamide/acetochlor, with the S-enantiomer inducing a much greater production of antioxidant defense enzymes (superoxide dismutase (SOD) and malondialdehyde (MDA)) and microcystins (MC). SOD and MC increased after treatment with the herbicides and these increases were dependent on the exposure time, whereas MDA showed no apparent change. The information provided in this work will be useful for understanding the toxicity mechanism and environmental behaviors of different amide herbicides (napropamide and acetochlor) in aquatic environments at the enantiomeric level. Additionally, analysis of chiral herbicides in aquatic system needs more attention to aide in the environmental assessment of chiral herbicides.
اظهر المزيد [+] اقل [-]Urban population exposure to tropospheric ozone: A multi-country forecasting of SOMO35 using artificial neural networks
2019
Antanasijević, Davor | Pocajt, Viktor | Perić-Grujić, Aleksandra | Ristic, Mirjana
Urban population exposure to tropospheric ozone is a serious health concern in Europe countries. Although there are insufficient evidence to derive a level below which ozone has no effect on mortality WHO (World Health Organization) uses SOMO35 (sum of means over 35 ppb) in their health impact assessments. Is this paper, the artificial neural network (ANN) approach was used to forecast SOMO35 at the national level for a set of 24 European countries, mostly EU members. Available ozone precursors’ emissions, population and climate data for the period 2003–2013 were used as inputs. Trend analysis had been performed using the linear regression of SOMO35 over time, and it has demonstrated that majority of the studied countries have a decreasing trend of SOMO35 values.The created models have made majority of predictions (≈60%) with satisfactory accuracy (relative error <20%) on testing, while the best performing model had R² = 0.87 and overall relative error of 33.6%. The domain of applicability of the created models was analyzed using slope/mean ratio derivate from the trend analysis, which was successful in distinguishing countries with high from countries with low prediction errors. The overall relative error was reduced to <14%, after the pool of countries was reduced based on the abovementioned criterion.
اظهر المزيد [+] اقل [-]Cardiovascular endothelial inflammation by chronic coexposure to lead (Pb) and polycyclic aromatic hydrocarbons from preschool children in an e-waste recycling area
2019
Zheng, Xiangbin | Huo, Xia | Zhang, Yu | Wang, Qihua | Zhang, Yuling | Xu, Xijin
Lead (Pb) and polycyclic aromatic hydrocarbon (PAH) exposure is positively associated with cardiovascular disease (CVD), and the possible potential mechanism may be caused by damage to the endothelium by modulation of inflammatory processes. No comprehensive research shows co-exposure of Pb and PAH on cardiovascular endothelial inflammation in electronic waste (e-waste) exposed populations. Given this, the aim of this study is to provide evidence for a relationship between Pb and PAH co-exposure and cardiovascular endothelial inflammation, in an e-waste-exposed population, to delineate the link between a potential mechanism for CVD and environmental exposure. We recruited 203 preschool children (3–7 years) were enrolled from Guiyu (e-waste-exposed group, n = 105) and Haojiang (reference group, n = 98). Blood Pb levels and urinary PAH metabolites were measured. Percentages of T cells, CD4⁺ T cells and CD8⁺ T cells, complete blood counts, endothelial inflammation biomarker (serum S100A8/A9), and other inflammatory biomarkers [serum interleukin (IL)-6, IL-12p70, gamma interferon-inducible protein 10 (IP-10)] levels were evaluated. Blood Pb, total urinary hydroxylated PAH (ΣOHPAH), total hydroxynaphthalene (ΣOHNap) and total hydroxyfluorene (ΣOHFlu) levels, S100A8/A9, IL-6, IL-12p70 and IP-10 concentrations, absolute counts of monocytes, neutrophils, and leukocytes, as well as CD4⁺ T cell percentages were significantly higher in exposed children. Elevated blood Pb, urinary 2-hydroxynaphthalene (2-OHNap) and ΣOHFlu levels were associated with higher levels of IL-6, IL-12p70, IP-10, CD4⁺ T cell percentages, neutrophil and monocyte counts. Mediator models indicated that neutrophils exert the significant mediation effect on the relationship between blood Pb levels and S100A8/A9. IL-6 exerts a significant mediation effect on the relationship between blood Pb levels and IP-10, as well as the relationship between urinary ΣOHFlu levels and IP-10. Our results indicate that children with elevated exposure levels of Pb and PAHs have exacerbated vascular endothelial inflammation, which may indicate future CVD risk in e-waste recycling areas.
اظهر المزيد [+] اقل [-]Metagenomics sheds light on the metabolic repertoire of oil-biodegrading microbes of the South Atlantic Ocean
2019
Appolinario, Luciana R. | Tschoeke, Diogo | Paixão, Raphael V.S. | Venas, Tainá | Calegario, Gabriela | Leomil, Luciana | Silva, Bruno S. | Thompson, Cristiane C. | Thompson, Fabiano L.
Unplanned oil spills during offshore oil production are a serious problem for the industry and the marine environment. Here we assess the biodegradation potential of marine microorganisms from three water depths in the Campos Basin (South Atlantic Ocean): (i) 5 m (surface), (ii) ∼80 m (chlorophyll maximum layer), and (iii) ∼1200 m (near the bottom). After incubating seawater samples with or without crude oil for 52 days, we used metagenomics and classic microbiology techniques to analyze microbial abundance and diversity, and measured physical-chemical parameters to better understand biodegradation processes. We observed increased microbial abundance and concomitant decreases in dissolved oxygen and hydrocarbon concentrations, indicating oil biodegradation in the three water depths treatments within approximately 27 days. An increase in metagenomic sequences of oil-degrading archaea, fungi, and bacteria (Alcanivorax, Alteromonas, Colwellia, Marinobacter, and Pseudomonas) accompanied by a significant increase in metagenomic sequences involved in the degradation of aromatic compounds indicate that crude oil promotes the growth of microorganisms with oil degradation potential. The abundance of genes involved in biodegrading benzene, toluene, ethylbenzene, xylene, alkanes, and poly-aromatic hydrocarbons peaked approximately 3 days after oil addition. All 12 novel metagenome-assembled genomes contained genes involved in hydrocarbon degradation, indicating the oil-degrading potential of planktonic microbes in the Campos Basin.
اظهر المزيد [+] اقل [-]Insights into the uptake, elimination and accumulation of microplastics in mussel
2019
Fernández, Beatriz | Albentosa, Marina
The majority of plastics present in the marine environment are microplastics (MPs, <5 mm). Suspension filter feeders are susceptible species to MPs ingestion. Once ingested MPs can be eliminated packed in fecal pellets, or they can be accumulated within tissues, and likely be transferred along the food web. The research on MPs is hampered by the difficulty on their quantification and the lack of standardized methodologies. Indeed, limited information exits about the capacity of marine organisms to ingest, accumulate and eliminate MPs. In this work we investigated the uptake, elimination and accumulation of MPs (irregularly shaped particles of high density polyethylene, ≤22 μm) in mussel. Mussels were exposed to two concentrations of MPs (2 and 4 mm3 l−1), and their uptake, elimination and accumulation in digestive gland was investigated. The results showed that the uptake of MPs increased at the high concentration tested, and that mussels cleared MPs at the same extent than a food item (microalgae) of similar size. Small MPs (2–4 μm) were less efficiently cleared than the larger ones. Large MPs (>10 μm) were faster eliminated than the smaller ones. The global balance showed that after 6 days of depuration mussels eliminated ≈85% of the MPs cleared, and that ≈2–6% of the MPs cleared remained in the digestive gland, essentially those <6 μm. We recorded a long retention time for MPs, contrasting with the lower times assumed to be necessary to empty mussel's gut before quantifying MPs. Our study emphasized the gap of knowledge on the feeding behaviour of mussels in relation to MPs, and the necessity to investigate it in different marine species, and under different exposure scenarios.
اظهر المزيد [+] اقل [-]Repeated detection of polystyrene microbeads in the Lower Rhine River
2019
Mani, Thomas | Blarer, Pascal | Storck, Florian R. | Pittroff, Marco | Wernicke, Theo | Burkhardt-Holm, Patricia
Microplastics are emerging pollutants in water bodies worldwide. The environmental entry areas must be studied to localise their sources and develop preventative and remedial solutions. Rivers are major contributors to the marine microplastics load. Here, we focus on a specific type of plastic microbead (diameter 286–954 μm, predominantly opaque, white–beige) that was repeatedly identified in substantial numbers between kilometres 677 and 944 of the Rhine River, one of Europe's main waterways. Specifically, we aimed (i) to confirm the reported abrupt increase in microbead concentrations between the cities of Leverkusen and Duisburg and (ii) to assess the concentration gradient of these particles along this stretch at higher resolution. Furthermore, we set out (iii) to narrow down the putative entry stretch from 81.3 km, as reported in an earlier study, to less than 20 km according to our research design, and (iv) to identify the chemical composition of the particles and possibly reveal their original purpose. Surface water filtration (mesh: 300 μm, n = 9) at regular intervals along the focal river stretch indicated the concentration of these spherules increased from 0.05 to 8.3 particles m−3 over 20 km. This spot sampling approach was supported by nine suspended solid samples taken between 2014 and 2017, encompassing the river stretch between Leverkusen and Duisburg. Ninety-five percent of microbeads analysed (202/212) were chemically identified as crosslinked polystyrene-divinylbenzene (PS-DVB, 146/212) or polystyrene (PS, 56/212) via Raman or Fourier-transform infrared spectroscopy. Based on interpretation of polymer composition, surface structure, shape, size and colour, the PS(-DVB) microbeads are likely to be used ion-exchange resins, which are commonly applied in water softening and various industrial purification processes. The reported beads contribute considerably to the surface microplastic load of the Rhine River and their potential riverine entry area was geographically narrowed down.
اظهر المزيد [+] اقل [-]Spatial distribution of lead contamination in soil and equipment dust at children's playgrounds in Beijing, China
2019
Peng, Tianyue | O'Connor, David | Zhao, Bin | Jin, Yuanliang | Zhang, Yunhui | Tian, Li | Zheng, Na | Li, Xiaoping | Hou, Deyi
Lead contamination is widespread across China, posing a serious public health concern. In quantifying child lead exposure, established health risk assessment (HRA) approaches often take into account residential soil lead levels. However, this may not constitute a significant exposure source for children in urban mainland China, where the population mainly dwell in high-rise buildings without back or front yards. In this setting, children's playgrounds may represent a more probable exposure source. The present study analyzed lead levels in settled dust on playground equipment and in surficial soils at 71 playgrounds in Beijing, China. Our results reveal that the average playground dust lead concentration was 80.5 mg/kg, more than twice the average soil lead concentration of 36.2 mg/kg. It was found that there are differences in statistical and spatial distributions for lead in playground dust and soils. Lead levels in equipment dust were largely consistent across Beijing, with elevated levels detected at locations in the main city area, the newly developed Tongzhou District, and the rural counties. Whereas average soil lead concentrations were higher at playgrounds in the main city area than other areas of Beijing. Statistical analysis suggests that the lead content in dust and soil may derive from different natural and anthropogenic sources. Equipment dust lead may be associated with long-distance atmospheric transportation and deposition. Whereas lead in soil is more likely to be associated with local traffic. This study also found that, in certain areas of Beijing, the risk of blood lead levels (BLLs) exceeding safe levels was up to 6 times higher when based on dust exposure than when based on playground soil exposure. The results of this study suggests that HRA undertaken for children in urban mainland China should pay closer attention to children's playgrounds as a lead exposure source, and, in particular, playground equipment dust.
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