The effect of different Na concentrations on the fate of trace metals (Cd, Cu, Ni, Zn) in standard OECD soil was evaluated by performing soil leaching column experiments. Five Na concentrations added in synthetic irrigation water (0, 1, 5, 10, 50 mM) were studied in order to evaluate the fate of the metals contained in both the irrigation water leachate and the soil layer. In all experiments, metals mostly accumulated on the top soil layer (0–0.5 cm), at variable concentrations according to the Na content in the artificial irrigation water. Nevertheless, concentration peaks of metal contamination occurred at different sampling time in the soil leachates depending on the metal and on influent water sodicity. Peaks of metals in the leachate appeared simultaneously with the release of organic matter and/or release of Al, suggesting significant involvement of colloids in metals transport. Sodium concentration (10–50 mM) was demonstrated to highly reduce colloidal mobilization leading to the accumulation of more than 95% of the influent metal in the top soil layer. Conversely, low Na concentrations (1–5 mM) favored colloidal transport leading to the recovery of metals in the soil leachates.

3,3′-dichlorobiphenyl (PCB-11) is an emerging PCB congener widely detected in environmental samples and human serum, but its toxicity potential is poorly understood. We assessed the effects of three concentrations of PCB-11 on embryotoxicity and Aryl hydrocarbon receptor (Ahr) pathway interactions in zebrafish embryos (Danio rerio). Wildtype AB or transgenic Tg(gut:GFP) strain zebrafish embryos were exposed to static concentrations of PCB-11 (0, 0.2, 2, or 20 μM) from 24 to 96 h post fertilization (hpf), and gross morphology, Cytochrome P4501a (Cyp1a) activity, and liver development were assessed via microscopy. Ahr interactions were probed via co-exposures with PCB-126 or beta-naphthoflavone (BNF). Embryos exposed to 20 μM PCB-11 were also collected for PCB-11 body burden, qRT-PCR, RNAseq, and histology. Zebrafish exposed to 20 μM PCB-11 absorbed 0.18% PCB-11 per embryo at 28 hpf and 0.61% by 96 hpf, and their media retained 1.36% PCB-11 at 28 hpf and 0.84% at 96 hpf. This concentration did not affect gross morphology, but altered the transcription of xenobiotic metabolism and liver development genes, impeded liver development, and increased hepatocyte vacuole formation. In co-exposures, 20 μM PCB-11 prevented deformities caused by PCB-126 but exacerbated deformities in co-exposures with BNF. This study suggests that PCB-11 can affect liver development, act as a partial agonist/antagonist of the Ahr pathway, and act as an antagonist of Cyp1a activity to modify the toxicity of compounds that interact with the Ahr pathway.

Anaerobic ammonium oxidation (anammox) is recognized as an important bioprocess for nitrogen removal, yet little is known about the associated microbial communities in urban river networks which are intensively disturbed by human activity. In the present study, we investigated the community composition and abundance of anammox bacteria in the urban river network of Shanghai, and explored their potential correlations with nitrogen removal activities and the environmental parameters. High biodiversity of anammox bacteria was detected in the sediment of urban river networks, including Candidatus Brocadia, Scalindua, Jettenia, and Kuenenia. Anammox bacterial abundance ranged from 3.7 × 10⁶ to 3.9 × 10⁷ copies g⁻¹ dry sediment based on 16S rRNA gene, which was strongly correlated to the metabolic activity of anammox bacteria (P < 0.01). A strong linkage between anammox bacteria and denitrifiers was detected (P < 0.05), implying a potential metabolic interdependence between these two nitrogen-removing microbes was existed in urban river networks. Sediment ammonium (NH₄⁺) made a significant contribution to the anammox bacterial community-environment relationship, while anammox bacterial abundance related significantly with sediment total organic carbon (TOC) and silt contents (P < 0.05). However, no statistically significant correlation was observed between cell-specific anammox rate and the measured environmental factors (P > 0.05). In general, the community composition and abundance of anammox bacteria in different hierarchies of the river network was homogeneous, without significant spatial variations (P > 0.05). These results provided an opportunity to further understand the microbial mechanism of nitrogen removal bioprocesses in urban river networks.

Once released into the environment, engineered nanomaterials can significantly influence the transformation and fate of organic contaminants. To date, the abilities of composite nanomaterials to catalyze environmentally relevant abiotic transformation reactions of organic contaminants are largely unknown. Herein, we investigated the effects of two nanocomposites – consisting of anatase titanium dioxide (TiO2) with different predominantly exposed crystal facets (i.e., {101} or {001} facets) anchored to hydroxylated multi-walled carbon nanotubes (OH-MWCNT) – on the hydrolysis of 1,1,2,2-tetrachloroethane (TeCA), a common groundwater contaminant, at ambient pH (6, 7 and 8). Both OH-MWCNT/TiO2 nanocomposites were more effective in catalyzing the dehydrochlorination of TeCA than the respective component materials (i.e., bare OH-MWCNT and bare TiO2). Moreover, the synergistic effect of the two components was evident, in that the incorporation of OH-MWCNT increased the TeCA adsorption capacity of the nanocomposites, significantly enhancing the catalytic effect of the deprotonated hydroxyl and carboxyl groups on nanocomposite surfaces, which served as the main catalytic sites for TeCA hydrolysis. The findings may have important implications for the understanding of the environmental implications of composite nanomaterials and may shed light on the design of high-performance nanocomposites for enhanced contaminant removal.

Modeling for the toxicity of ionic liquids (ILs) is necessary to fill data gaps for untested chemicals and to understand the relevant mechanisms at the molecular level. In order for many researchers to easily predict toxicity and/or develop some prediction model, simple method(s) based on a single parameter should be proposed. Therefore, previously our group developed a comprehensive toxicity prediction model with unified linear free-energy relationship descriptors to address the single parameter for predicting the toxicities, as follows (Cho et al., 2016b).Log 1/toxicity in the unit of mM= (2.254 Ec – 2.545 Sc + 0.646 Ac – 1.471 Bc + 1.650 Vc + 2.917 J+ – 0.201 Ea + 0.418 Va + 0.131 J−) – 0.709.It is considered that the model can calculate the general toxicological effect of ILs in parenthesis, as it was developed on the basis of numerous toxic effects i.e., 58 toxicity testing methods and about 1600 data points. In order to check the hypothesis, the values calculated by the model were correlated with four different datasets from insect cell line (Spodoptera frugiperda 9), earthworm (Eisenia fetida), nematode (Caenorhabditis elegans), and fish (Danio rerio). The results clearly showed that the calculated values are in good agreement with each dataset. In the case of S. frugiperda 9 cells, the calculated parameters were correlated with log1/LC50 values, measured after 24 h and 48 h incubation, in R2 of 0.67 and 0.88, respectively. The R2 values for the earthworm, nematode, and fish were 0.88, 0.96, and 0.94–0.95, respectively. This study confirmed that the comprehensive model can be simply and accurately used to predict toxicity of ILs.

Herein, we investigated the effects of di-n-butyl phthalate (DBP) on photosynthesis, ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) content, oxidative damage, and biomass accumulation of different tissues in wheat (Triticum aestivum L) planted in cinnamon soils. The photosynthetic or fluorescence parameters (except for the intercellular carbon dioxide concentration), chlorophyll content, RuBisCO content, and biomass of roots, stems, and leaves decreased at the seedling, jointing, and booting stages under the stress of DBP. Compared with the control, the content of superoxide anions and hydrogen peroxide in the roots, stems, and leaves increased with increasing DBP concentrations at the seedling, jointing, and booting stages. The activities of superoxide dismutase (SOD) and catalase (CAT) in the roots, stems, and leaves increased under the 10 and 20 mg kg−1 DBP treatments; however, no significant changes were observed under the 40 mg kg−1 DBP treatment at the seedling stage (except for the SOD activity in roots). The increase in SOD and CAT activities in the roots, stems, and leaves with increasing DBP concentration at the jointing and booting stages suggested that an increase in the activities of these antioxidant enzymes may play an important role in defending against excess reactive oxygen species under DBP stress. The biomass of wheat roots, stems, and leaves decreased with an increase in DBP concentration, which was presumably caused by a decrease in photosynthesis and RuBisCO. The effect of DBP on wheat roots, stems, and leaves decreased with wheat growth.

Organic pollutants in the Arctic seas have been of concern to many researchers; however, the vast dynamic marine water poses challenges to their comprehensive monitoring within appropriate spatial and temporal scales in the Arctic. In this study, on-board passive sampling of organic pollutants using a self-developed device coupled with triolein-embedded cellulose acetate membranes (TECAMs) was performed during an Arctic cruise. The TECAM extracts were used for target analysis of organophosphorus flame retardants (PFRs), and non-target screening of persistent, bioaccumulative, and toxic (PBT) contaminants using two-dimensional gas chromatography with time-of-flight mass spectrometry (GC × GC-TOFMS). Sixteen chemicals were screened out as PBT contaminants from the 1500 features in the non-target analysis and further identified. Consequently, two chlorinated PFRs (tris(chloroisopropyl)phosphate and tris(1,3-dichloroisopropyl)phosphate) and four PBT contaminants (4-tert-butylphenol, 2-isopropylnaphthalene, 1,1,3-trimethyl-3-phenylindane, and 1-phenylnonan-1-one) were accurately quantified, with the temporally and spatially integrated concentrations ranging from 0.83 ng L⁻¹ to 20.82 ng L⁻¹ in the seawaters. Sources and transport of the contaminants were studied, and ocean current transport (West Spitsbergen Current, WSC) and local sources (human settlement, Arctic oil exploitation, and petroleum fuel emissions) were found to contribute to the presence of the different contaminants. Finally, annual transport fluxes of the contaminants from the North Atlantic to the Arctic Ocean by WSC were estimated, and the results indicate that their hazard to the Arctic should be concerned.

Bisphenol A (BPA) is widely used in consumer products and is a potential endocrine disruptor linked with abnormal development of male reproductive tract. However, its action and its effects on the pathways in the development of male gonad are still unclear. Here we report that effects of BPA exposure during gestation on male gonad development. Sprague-Dawley rats were gavaged daily with BPA (0, 4, 40, and 400 mg/kg body weight) from gestational day 12 to day 21. BPA dose-dependently decreased serum testosterone levels (0.45 ± 0.08 ng/ml and 0.32 ± 0.08 ng/ml for 40 and 400 mg/kg BPA, respectively) versus the control level (1.11 ± 0.22 ng/ml, Mean ± SE). BPA lowered Leydig cell Insl3 and Hsd17b3 mRNA and their protein levels at doses of 40 and 400 mg/kg. BPA also lowered Leydig cell (Lhcgr, Cyp11a1, and Cyp17a1) and Sertoli cell (Amh) mRNA and their protein levels at 400 mg/kg. BPA decreased fetal Leydig cell number via inhibiting their proliferation, but it did not affect fetal Sertoli cell number. In conclusion, the current study shows that in utero exposure to BPA inhibits fetal Leydig and Sertoli cell differentiation, possibly disrupting the development of male reproductive tract.

Melatonin (Mel) serves as an important signalling molecule in various aspects of stress tolerance in plants. However, the function of Mel in pesticide metabolism remains unknown. Here, selecting the widely used fungicide carbendazim (MBC) as the model, we found that exogenous Mel had the ability to alleviate pesticide phytotoxicity and residues in tomato as well as in some other vegetables. Additionally, overexpression of the Mel biosynthetic gene caffeic acid O-methyltransferase 1 (COMT1) significantly enhanced the capacity of the tomato to reduce MBC phytotoxicity and residue. This outcome was mainly because of the Mel-induced antioxidant capability, as well as the key detoxification process. Indeed, levels of reactive oxygen species (ROS) and lipid peroxides significantly decreased after applying exogenous Mel or overexpressing COMT1, which resulted from direct ROS scavenging, and increased Mel levels significantly enhanced antioxidant enzymatic activity. More importantly, Mel activated the ascorbate-glutathione cycle to participate in glutathione S-transferase-mediated pesticide detoxification. A grafting experiment showed that rootstocks from COMT1 transgenic plants increased the Mel accumulation of wild-type scions, resulting in MBC metabolism in the scions. To our knowledge, this is the first report providing evidence of Mel-induced pesticide metabolism, which provides a novel approach for minimizing pesticide residues in crops by exploiting plant self-detoxification mechanisms.

MicroRNAs (miRNAs) are a class of small non-coding RNA that control multiple biological processes through negative post-transcriptional regulation of gene expression. Recently a role of miRNAs in the response of aquatic organisms to environmental toxicants emerged. Toxicant-induced changes in miRNA expression might then represent novel biomarkers to evaluate the health status of these organisms. In this study, we aimed to identify the miRNA repertoire in the liver of the European eel Anguilla anguilla and to compare their differential expression between a polluted site located in the Gironde Estuary and a pristine site in Arcachon Bay (France).A total of 299 mature miRNAs were identified. In polluted water, 19 miRNAs were up-regulated and 22 were down-regulated. We predicted that these differentially expressed miRNAs could target 490 genes that were involved in ribosome biogenesis, response to hormones, response to chemical and chromatin modification. Moreover, we observed only few examples (29) of negative correlation between the expression levels of miRNAs and their targets suggesting that, in the system studied, miRNAs might not only regulate gene expression directly by degrading mRNA but also by inhibiting protein translation or by regulating other epigenetic processes.This study is the first example of in situ investigation of the role of miRNAs in the response of a fish species to water quality. Our findings provide new insights into the involvement of epigenetic mechanisms in the response of animals chronically exposed to pollution and pave the way for the utilization of miRNAs in aquatic ecotoxicology.