This study aims to use ultraviolet (UV) irradiation to decompose polybrominated diphenyl ethers (PBDEs) in the elutes and then reuse the surfactants. The results indicate that UV can remove 2,2′,4,4′-tetrabrominated diphenyl ether (BDE-47) from surfactant eluents and Triton X series surfactants also can remove BDE-47 from the soil. Triton X-100 (TX-100) is the most promising surfactant during the washing and photodegradation processes. Quench experiments suggest that both ¹O₂ and OH• were involved in the TX-100 decomposition but only ¹O₂ is responsible for the degradation of BDE-47. In analysis of the photoproducts of BDE-47 by Gas Chromatography Mass Spectrum (GC-MS) and Liquid Chromatography High Resolution Mass Spectrometry (LC-HRMS), BDE-47 was mainly debrominated to the lower-brominated BDEs and then oxidized to ring-opening products. The little loss of TX-100 can mainly be attributed to the breakage of polyethylene oxide (PEO) chain. Nevertheless, the washing wastes treated by UV light can exhibit higher solubility for BDE-47 than before, indicating they can be reused for BDE-47 removal from soil. The toxicity assessment experiments were performed using Escherichia coli (E.coli) as an indicator. The results indicate that the removal of BDE-47 by UV irradiation can reduce the toxicity of eluent.

The seasonal variation and distribution among different matrices of endocrine-disrupting chemicals (EDCs) were investigated in the eutrophic water ecosystem of the Pearl River Delta, Guangdong, China. The chlorophyll a (Chl a) levels were generally higher in summer than in spring; however, the concentrations of 4-tert-octylphenol (OP), 4-nonylphenol (NP), and bisphenol A (BPA) in surface water were generally higher in spring (oligotrophic) than in summer (eutrophic). The levels of EDCs in SPM were lower in spring than in summer, a pattern seen in the seasonal variation of Chl a and particulate organic carbon (POC). The seasonal variations of EDCs in water bodies with different levels of eutrophication were analyzed in several dimensions including sediment, POC, algae and fish bile. The log Kₒc for SPM/water was higher in summer than in spring. The log Kₒc values for NP, OP, and BPA exhibited the following trends between matrices: colloid/water > sediment/water > SPM/water > algae/water, colloid/water > sediment/water > algae/water > SPM/water, and colloid/water > algae/water > sediment/water > SPM/water. The EDCs levels were different in fish tissues with the order bile > liver > muscle, with the concentrations being an order of magnitude higher in bile than in liver and an order of magnitude higher in liver than in muscle. The sequence of the bioconcentration factor (log BCF) for bile/water and liver/water was NP < OP < BPA in eutrophic conditions, but NP > OP > BPA in oligotrophic conditions. The order in eutrophic conditions was the same as the log BCF and log Kₒc for algae/water, indicating that the accumulation of EDCs in water bodies could be affected by algae, which could be one of the reasons of the seasonal variation of EDCs in water.

In recent years, numerous studies paid more attention to the molecular mechanisms associated with fluoride toxicity. However, the detailed mechanisms of fluoride immunotoxicity in bovine neutrophils remain unclear. Neutrophil extracellular traps (NETs) is a novel immune mechanism of neutrophils. We hypothesized that sodium fluoride (NaF) can trigger NETs activation and release, and investigate the related molecular mechanisms during the process. We exposed peripheral blood neutrophils to 1 mM NaF for 120 min in bovine neutrophils. The results showed that NaF exposure triggered NET-like structures decorated with histones and granule proteins. Quantitative measurement of NETs content correlated positively with the concentration of NaF. Mechanistically, NaF exposure increased reactive oxygen species (ROS) levels and phosphorylation levels of ERK, p38, whereas inhibiting the activities of superoxide dismutase (SOD) and catalase (CAT) compared with control neutrophils. NETs formation is induced by NaF and this effect was inhibited by the inhibitors diphenyleneiodonium chloride (DPI), U0126 and SB202190. Our findings described the potential importance of NaF-triggered NETs related molecules, which might help to extend the current understanding of NaF immunotoxicity.

Being signaling molecules, nitric oxide (NO) and hydrogen sulfide (H₂S) can mediate a wide range of physiological processes caused by plant metal toxicity. Moreover, legume-rhizobium symbiosis has gained increasing attention in mitigating heavy metal stress. However, systematic regulatory mechanisms used for the exogenous application of signaling molecules to alter the resistance of legume-rhizobium symbiosis under metal stress are currently unknown. In this study, we examined the exogenous effects of sodium nitroprusside (SNP) as an NO donor additive and sodium hydrosulfide (NaHS) as a H₂S donor additive on the phytotoxicity and soil quality of alfalfa (Medicago sativa)-rhizobium symbiosis in lead/cadmium (Pb/Cd)-contaminated soils. Results showed that rhizobia inoculation markedly promoted alfalfa growth by increasing chlorophyll content, fresh weight, and plant height and biomass. Compared to the inoculated rhizobia treatment alone, the addition of NO and H₂S significantly reduced the bioaccumulation of Pb and Cd in alfalfa-rhizobium symbiosis, respectively, thus avoiding the phytotoxicity caused by the excessive presence of metals. The addition of signaling molecules also alleviated metal-induced phytotoxicity by increasing antioxidant enzyme activity and inhibiting the level of lipid peroxidation and reactive oxygen species (ROS) in legume-rhizobium symbiosis. Also, signaling molecules improved soil nutrient cycling, increased soil enzyme activities, and promoted rhizosphere bacterial community diversity. Both partial least squares path modeling (PLS-PM) and variation partitioning analysis (VPA) identified that using signaling molecules can improve plant growth by regulating major controlling variables (i.e., soil enzymes, soil nutrients, and microbial diversity/plant oxidative damage) in legume-rhizobium symbiosis. This study offers integrated insight that confirms that the exogenous application of signaling molecules can enhance the resistance of legume-rhizobium symbiosis under metal toxicity by regulating the biochemical response of the plant-soil system, thereby minimizing potential health risks.

Remediation of heavy metal contaminated soils remains a global challenge. Here, low-molecular-weight organic acids were used to extract Cu and Zn from polluted soils, and the extracted heavy metals were subsequently adsorbed by activated carbon electrodes. The electrochemical adsorption mechanism as well as the influence of pH, organic acid type and voltage were investigated, and the soil remediation effect was further evaluated by the cultivation of rape. After extraction by citrate at initial pH 8.3 and electrochemical adsorption at 0.9 V for 7 d, the concentrations of total and bioavailable Cu in soils decreased from 1090 to 281 to 391 and 52 mg kg⁻¹, and those of Zn decreased from 262 to 39 to 208 and 30 mg kg⁻¹, respectively. Cu and Zn ions were mainly electrochemically adsorbed on the carbon cathode and anode, respectively, resulting in decreases of their concentrations to below 1 mg L⁻¹ in the leachate. The presence of organic acids improved the remediation performance in the order of citrate > oxalate > acetate. The decrease in the initial pH of citrate solution enhanced the removal rate of Zn, while seemed to have no effect on that of Cu. The removal capacity for heavy metals decreased with decreasing cell voltage from 0.9 to 0.3 V. In the rape cultivation experiment, the Cu and Zn contents in shoot and root were decreased by more than 50%, validating the soil remediation effect. The present work proposes a facile method for heavy metal removal from contaminated soils.

Antibiotic and heavy metal pollution of aquatic environments are issues of serious concern, and the macrophyte Myriophyllum aquaticum may provide a viable solution for the removal of these contaminants. However, the toxic effects of coexisting tetracyclines (TCs) and Cu(II) on this plant species are currently unclear. In the present study, we constructed wetland microcosms planted with M. aquaticum and spiked these with three TCs (tetracycline, oxytetracycline, and chlortetracycline) and Cu(II) at concentrations ranging from 100 to 10,000 μg/L to investigate how Cu(II) influences the growth and tolerance responses of plants to TCs. After 12 weeks, we found that TCs had accumulated in the plants, and that plant growth and characteristics were significantly affected by the levels of both TCs and Cu(II). While low Cu(II) levels had a synergistic effect on the accumulation of TCs, high levels were observed to reduce accumulation. However, low levels of TCs and Cu(II) had a hormesis effect on plant growth, with plant biomass and leaf chlorophyll content decreasing and the malondialdehyde content and activities of antioxidant enzymes gradually increasing with an increase in TC dosage. The coexistence of low levels of Cu(II) was, however, found to alleviate these adverse effects. Principal component analysis revealed a close relationship among plant biomass, chlorophyll content, malondialdehyde content, and antioxidant enzyme activities. Considering that the Cu/TC ratio was shown to markedly affect M. aquaticum growth, the respective proportions of these pollutants should be taken into consideration in the future design of constructed wetlands.

Forest understory plant communities in the eastern United States are often diverse and are potentially sensitive to changes in climate and atmospheric inputs of nitrogen caused by air pollution. In recent years, empirical and processed-based mathematical models have been developed to investigate such changes in plant communities. In the study reported here, a robust set of understory vegetation response functions (expressed as version 2 of the Probability of Occurrence of Plant Species model for the United States [US-PROPS v2]) was developed based on observations of forest understory and grassland plant species presence/absence and associated abiotic characteristics derived from spatial datasets. Improvements to the US-PROPS model, relative to version 1, were mostly focused on inclusion of additional input data, development of custom species-level input datasets, and implementation of methods to address uncertainty. We investigated the application of US-PROPS v2 to evaluate the potential impacts of atmospheric nitrogen (N) and sulfur (S) deposition, and climate change on forest ecosystems at three forested sites located in New Hampshire, Virginia, and Tennessee in the eastern United States. Species-level N and S critical loads (CLs) were determined under ambient deposition at all three modeled sites. The lowest species-level CLs of N deposition at each site were between 2 and 11 kg N/ha/yr. Similarly, the lowest CLs of S deposition, based on the predicted soil pH response, were less than 2 kg S/ha/yr among the three sites. Critical load exceedance was found at all three model sites. The New Hampshire site included the largest percentage of species in exceedance. Simulated warming air temperature typically resulted in lower maximum occurrence probability, which contributed to lower CLs of N and S deposition. The US-PROPS v2 model, together with the PROPS-CLF model to derive CL functions, can be used to develop site-specific CLs for understory plants within broad regions of the United States. This study demonstrates that species-level CLs of N and S deposition are spatially variable according to the climate, light availability, and soil characteristics at a given location. Although the species niche models generally performed well in predicting occurrence probability, there remains uncertainty with respect to the accuracy of reported CLs. As such, the specific CLs reported here should be considered as preliminary estimates.

The biological effects of nanoplastics are a growing concern. However, most studies have focused on exposure to high concentrations or short-term exposure. The potential effects of exposure to low environmental nanoplastic concentrations over the long-term and across multiple generations remain unclear. In the present study, Daphnia pulex was exposed over three 21-day generations to a typical environmental nanoplastic concentration (1 μg/L) and the effects were investigated at physiological (growth and reproduction), gene transcription and enzyme activity levels. Chronic exposure did not affect the survival or body length of D. pulex, whereas the growth rate and reproduction were influenced in the F2 generation. Molecular responses indicated that environmental nanoplastic concentrations can modulate the response of antioxidant defenses, vitellogenin synthesis, development, and energy production in the F0-F1 generations, and prolongation resulted in inhibitory effects on antioxidant responses in F2 individuals. Some recovery was observed in the recovery group, but reproduction and stress defenses were significantly induced. Taken together, these results suggest that D. pulex recovery from chronic exposure to nanoplastic may take several generations, and that nanoplastics have potent long-term toxic effects on D. pulex. The findings highlight the importance of multigenerational and chronic biological evaluations to assess risks of emerging pollution.

Due to high global demand, large amounts of abbattoir waste are generated from pork production. Mismanagement of abattoir waste on agricultural lands can result in soil and water contamination with pathogens and contaminants like metals and nutrients. Therefore, possible effects on soil organisms prior to application should be evaluated. Thus, the aim of this study was to determine the effects of fresh pig abattoir waste (PAWf) and waste after stabilization processes on E. andrei through tests of avoidance behavior, acute toxicity and chronic toxicity. In order to do this, the waste was evaluated fresh (i.e., non-treated), and after aerated composting (PAWa), natural composting (PAWn) and vermicomposting (PAWv). In addition, we used a natural soil with no history of agricultural use as control soil. The evaluation was based on avoidance behavior, mortality, initial and final earthworm weight, and reproduction, in addition to a set of enzyme assays formed by acetylcholinesterase, lipid peroxidation, catalase and glutathione S-transferase measured over time. The ecotoxicological results showed that PAWf and PAWa increased AChE activity at different experimental periods, while PAWn decreased activity at 14 days compared to the control. PAWf and PAWa increased TBARS levels at 7 and 14 days, respectively. CAT activity decreased at 3, 7 and 14 days in PAWv, while GST activity increased at 3 days in PAWa and at 3 and 14 days in PAWf compared to the control. In the acute toxicity test, PAWa and PAWn had a toxic effect on E. andrei, resulting in 100% mortality at 14 days of exposure. Based on our findings, pig abattoir waste should undergo vermicomposting prior to agricultural application to soils.

Mosses are frequently used to monitor atmospheric metal contamination but few studies on metal adsorption under controlled conditions are available. Here, the accumulation of the heavy metals copper and zinc was studied in the acrocarp moss Atrichum undulatum. An in vitro culture of A. undulatum was established and the same line, size and equally old remets were exposed to six different treatments representing various metal exposure times and washing scenarios as rain simulation. The metal treatments were done in copper and zinc salts (Cu-acetate, CuSO4, ZnSO4 and ZnCl2, respectively). Energy-Dispersive X-ray microanalysis (EDX) was employed to detect bound heavy metals on the moss plantlets. Element distribution in stems and leaves was measured separately. The aqueous solution of metal salts facilitated an adsorption of both elements in the moss tissue as compared to solid medium. Furthermore, A. undulatum can tolerate pollution of zinc and copper in a distinctive extent; our data point towards a higher zinc tolerance whereas copper is rather harmful. However, semi-quantitatively, less zinc was detected within the moss tissue compared to copper. Interestingly, a strong positive correlation between the accumulation of copper/zinc and iron, and a strong negative correlation between copper/zinc and magnesium, respectively, was documented.