The aim of studies was to identify how different morphological and biological characteristics influence spring barley (Hordeum vulgare L.) competitiveness against weeds in various organic and conventional farming locations. Do those traits have an influence on the yield and in which growing conditions the selection of genotypes for organic farming should be done? The plant traits related to competitiveness against weeds at the beginning of the vegetation period are important in breeding for organic agriculture. Ten different varieties were included in trials for three years in two organic and two conventional environments. The included varieties were divided in four groups with respect to time of release and adaptation to growing environments: old extensive varieties and landraces for low input agriculture, medium old varieties with good adaptation to unfavourable conditions, modern varieties for low input, and modern varieties for high input conditions. Results showed that such traits as productive tillering ability, development speed and plant height at the beginning of stem elongation stage provide good soil shading. The most of traits were negatively related to the grain yield. Selection of genotypes for traits which influence spring barley competitiveness against weeds, such as a growth habit in the tillering stage, development speed in tillering stage, plant height at the beginning of stem elongation, length of flag leaf and the width of flag leaf, and the plant height before harvest, may take place in conventional conditions as well as in organic conditions.

The objective of the study was to investigate the microbiological content of cow (Bos primigenius) milk in Latvian organic farms according to season and herd size with a purpose to detect their impact on the distribution of mastitis causing pathogens in milk. Samples were collected in 14 organic dairy farms of Latvia, 4 times through 2012: in winter, autumn, spring and summer period. Raw milk samples (n=564) obtained from cow composite milk were studied. The samples were divided into three groups in accordance with the number of cows in the cow-shed: A (3-30), B (31-60) and C (61-124). The total colony count (TTC) and the isolation of mastitis causing bacteria were analysed using standard methods. Bacterial growth occurred in 90.4% of samples. Isolated microorganisms belonged to 35 species, and the following bacteria were the most prevalent agent, including Coagulase negative staphylococci in 29.4%, Staphylococcus aureus in 24.1%, Kocuria kristinae in 12.9%, and Enterobacteriaceae spp. in 10.3% out of 564 milk samples. Depending on the season, the average number of TCC was the lowest in summer (4.66 ± 4.01 log10 CFU mLE-1), moderately higher in spring and winter (4.72 ± 4.18 and 4.82 ± 3.54 log10 CFU mLE-1, respectively), but significantly higher in autumn (5.43 ± 4.80 log10 CFU mL-E1). Coliforms were not isolated from group A herds instead of B (3.1%) and C (4.6%). Occurrence of Staphylococcus aureus was noticeably higher in B (19.1%) and C (20.8%) than in A (12.6%) herds. Most of group A herds met the milk quality requirements, while group C herds produced more contaminated milk.

Field trials with the spring wheat (Triticum aestivum L.) were carried out on the experimental fields of Estonian University of Life Sciences in 2011. The yield quality (volume weight, 1000 kernel weight, falling number, gluten content, gluten index, gluten content in dry matter) of spring wheat was studied in cultivar ‘Vinjett’. The wheat was part of the five-year crop rotation experiment where red clover (Trifolium pratense L.), spring wheat, peas (Pisum sativum L.), potato (Solanum tuberosum L.) and barley (Hordeum vulgare L.) undersown with red clover were following each other. There were three treatments which followed the crop rotation. In one treatment mineral fertilizers and pesticides were used, but other two treatments were organic without any synthetic agrochemicals. In mineral fertilizing treatments, the background of P25 and K95 kg ha-1 the N amount varied from 0 to 150 kg haE-1. Herbicides Sekator and MCPA 750 were used in conventional part. The aim of this research was to study red clover’s after-effect and different N fertilizer amounts’ influence on the spring wheat yield quality. In treatments where no mineral fertilizers were used and which only followed the red clovers after effect had higher gluten index and gluten content in dry matter. Higher mineral nitrogen amounts and organic treatments decreased spring wheat 1000 kernel weights and volume weights. The wet gluten content and falling number increased with increase of the amount of mineral N.

Ecological farming is relevant to protected areas. The aim of the study is to perform an analysis of farms applying organic farming measures as well as the analysis of the change of their areas in Aukštadvaris and Krekenava regional parks (RP). The object of the investigation - the farms of Aukštadvaris and Krekenava regional parks. The study is carried out to determine how many farms, forming the regional parks‘ territory, are managed according to the principles of organic farming. The change of the above- mentioned farming area for 2009-2011 is analyzed as well. Paluknys and Naujamiestis subdistricts, not incorporated into the territory of regional parks, were chosen for the comparison analysis. The analysis of the 2009 – 2011 period showed that the declared areas in Krekenava RP have decreased, and the area of farms applying organic measures has increased by 81.69 hectares (nearly 2.5 times). The number of organic farms increased by 1.5 times in Aukštadvaris RP over the period of 2009-2011, and the area has increased by 187.36 ha. The percentage calculations revealed that in 2011 the area of farms applying organic farming measures in Aukštadvaris RP made up 18.89 per cent of the total area declared, the number of farms - 6.61 per cent. The area of farms applying organic farming measures in Krekenava RP was only 1.71 per cent, measures were applied only by 1.89 per cent of all the declared.

The main purpose of the research was to investigate the physically-chemical parameters of organic and conventional hull-less barley (Hordeum vulgare) harvested in Latvia. In the experiment the following hull-less barley harvested in 2012 from the State Priekuli Plant Breeding Institute (Latvia) was used: ‘Irbe’, PR4651, PR3808.21 and PR5099. The following quality parameters of grain – protein, starch, the bulk density and β-glucan content – were measured by standard methods – Infratec™ 1241 Grain Analyser (Denmark) corresponding to ISO 12099; thousand-grain weight (TGW) (ISO 520:2010); the falling number (ISO 3093:2009); moisture content (LVS 272:2000) were also determined. In the present experiments no significant differences in protein, moisture, starch, β-glucan content and TGW were detected, however, significant differences in the bulk density of all analyzed hull-less barley samples were established. The lower analyzed parameter value of 780 ± 1 g LE-1 was obtained for the conventional and organic hull-less barley PR 5099. However, the higher bulk density value of 821 ± 1 g LE-1 was obtained in the conventional hull-less barley variety ‘Irbe’. No significant differences in the bulk density of conventional and organic hull-less barley ‘Irbe’, PR4651 and PR3808.21 were found. Significant differences in the falling number were detected in the analyzed hull-less barley samples harvested in the conventional and organic farming. The lower falling number value was obtained in the organic hull-less barley line PR 5009 – 81 ± 4 s, the higher in the conventional and organic hull-less barley line PR 3808.21 as 362 ± 5 s and 373 ± 2 s, respectively.

Products of organic origin used in agriculture to reduce the application of artificial pesticides and fertilizers are investigated world-wide now. Composting organic fertilizers, plant residue and household waste, results in new products of organic origin. In several countries (USA, Russia, Belorussia, and some others), the role of earthworms in organic waste processing and the possible further use of this processed product have been investigated. In Latvia, similar investigations are few but they are needed. A goal of our investigation was to investigate the impact of extracts made from the products of organic origin on potato (Solanum tuberosum) tuber yield in biological crop production system. A field experiment was established at the State Stende Cereals Breeding Institute in 2011, using an early-maturing potato variety ‘Borodjanskij Rozovij’ suitable for growing both in conventional and organic production systems. Tubers or plants were treated with peat elixir and earthworm bio-humus extract obtained at different temperatures: +45 °C and +95 °C, and their mixtures. In total, 18 treatments and untreated control variant were included in the experiment. Tubers were treated just prior to planting, but potato plants were treated three times in season. In the first production year, the obtained potato tuber yield ranged 26.16-45.38 t haE-1 in treated plots, and 35.27 t haE-1 in untreated control plot. Data were subjected to analysis of variance. In 2011, tuber yield was significantly dependent on organic extracts applied, which increased the yield significantly (p is lee than 0.05) in four treatments, but in the rest of them significantly decreased if compared to control.

The objective of the present study was to investigate the microbiological content of cows’ milk in Latvia’s organic farms with a purpose to detect potential microbiological threats in milk. Samples were collected in December 2011 at 12 biological dairy farms of Latvia. Raw milk samples (N=155) obtained from cow composite milk were studied. The total mesophilic aerobic and facultative anaerobic microorganisms (MAFAM), the presence of coliforms and coagulase-positive staphylococci, count of yeasts and moulds were analysed using standard methods. Of the sampled cows 50% had a low somatic cell count (SCC) (LESS THAN 200,000 cells mLE-1), 23% - high, but 27% had a very high SCC (greater than 500,000 cells mLE-1). The mean value of MAFAM in the samples with low, high and very high SCC was 4.7, 5.0 and 5.0 log10 colony forming units (cfu) mLE-1, respectively. The yeasts were present in 57% of milk samples with the mean concentration of 3.1 log10 cfu mLE-1. Moulds were found in 27% of all milk samples; their mean concentration was 4.4 log10 cfu mLE-1. Identified mould strains belonged to genera Absidia, Aspergillus, Geotrichum, Mucor and Penicillium. In cases of subclinical mastitis and latent mammary infection the most distributed mastitis pathogens were Staphylococcus aureus, Micrococcus kristinae, Bacillus cereus and coagulase negative staphylococci.

The research was carried out on ASU WRI grounds in Juodkiškes village in Endocalcari Endohypogleyic Cambisol (CMg-n-w-can). Research basis is 3 different field studies. In Variant I an organic cropping system was used with manure and without mineral fertilizers and pesticides, in II - organic-mineral cropping system with manure and mineral fertilizers, using pesticides, in Variant III - mineral cropping system with mineral fertilizers and pesticides. Minimum mineral nitrogen content in soil was in the organic cropping system, and the total minimal field crop capacity - in the mineral cropping system. However, it has the highest energy efficiency of this system (ETK - 13). Minimal N-NO3 - concentration in drainage water was in the mineral cropping system. In the organic-mineral and organic cropping systems concentration of this compound increases by 11 percent (to 21.8 mg l-1). The minimal concentration of ammonia nitrogen was under organic cropping conditions. The organic cropping system showed the highest runoff and maximum leaching of nitrogen compounds by drainage.

Organic farmers need varieties adapted to variable growing conditions, because in organic farms environmental conditions significantly vary in soil nutrient status, weed and disease pressure not only between years, but also among farms within a season. Modern varieties developed under high input agricultural systems do not always perform well under organic growing conditions. Therefore, farmers need such varieties of cereals that can adapt to variable growing conditions and maintain productivity. One of the ways to promote adaptation to environment is growing of variety mixtures. Field trials were conducted during 2014 – 2016 under organic and conventional farming systems in two locations with an aim to estimate the advantages of barley mixtures in respect of yield and its stability. The trial consisted of eight mixtures of barley varieties and breeding lines, components of mixtures in pure stand and three check varieties. The mixtures were combined by using two, three and five components. Three mixtures, each in one out of 11 environments significantly out–yielded, but one mixture had significantly lower yield than the average value of their components. Some mixtures showed a tendency to out–yield the average of components in both farming systems. All mixtures insignificantly out–yielded check varieties in 2015, but in 2016, yield was within the range between varieties with lowest and highest yield. The results of yield stability analysis showed that mixtures could be used as one of the approaches to stabilize yield – only one of eight mixtures had a lower yield than the average over all environments.

The assessment and conservation of agricultural biodiversity is one of the current goals of European Union policies, which are reflected in European Biodiversity Strategy. The biodiversity of soil seed bank and above-ground weed flora were investigated in spring cereals in organic and conventional farming system. The study was carried out between 2012 and 2014. Species of above-ground wild flora were counted on a surface of 1 square m in the field. Soil for seed bank evaluation was taken from the field and evaluated by seedling emergence method in a greenhouse. There were no significant differences in number of species between two communities. Biodiversity of weed community, described by Shannon’s diversity index and Simpson’s dominance index, was significantly better maintained in the soil seed bank than in above-ground flora in both farming systems. A strong, positive correlation between the number of species, Shannon’s diversity index, Simpson’s dominance index of soil seed bank and above-ground weed species was found in both farming systems, while abundance of seeds and above-ground weeds was significantly, positively correlated only in the conventional farming system. Qualitative composition of weed flora depended more on the type of community (soil seed bank or above-ground weed communities) than the farming system.