Tryptophan (Trp) is an essential amino acid for mammals since they cannot synthesise it. Wheat (Triticum aestivum L.) is a major source of dietary Trp, and wheat high in Trp would thus be very beneficial. This study investigated the impact of location and wheat variety on the content of free Trp and mineral nutrients in wheat grain. Four wheat cultivars from national variety tests on wheat with varying protein contents were selected from seven different locations of Sweden representing different climatic zones and soil contents of trace elements. Grain was analysed for Trp content using reversed phase HPLC. Mineral nutrients were analysed using inductively coupled plasma emission spectrometry. Wheat yield and grain content of nutrients and Trp differed significantly between sites. High N content in grain was correlated with a high content of Cu, Fe and S, and also Ca and Mg. However, it was correlated with a low content of K and Na. Trp content was positively correlated with Na and Zn content and negatively correlated with K/Na and K/(Na+Zn) ratio. The varieties differed in Trp content and the two ratios, but there were no significant differences in yields between the varieties at any location. The results indicated salt stress induction of Trp synthesis, which may protect wheat against salt stress and yield losses.

The mean content of minerals in grains varies significantly among different parts of the world as it depends on the type of grain growing conditions and fertilizer application. In this study, the content of minerals in the wheat, oat and barley grains of the crop of the year 2006 from different regions of Latvia were investigated. The grain samples were analysed for K, Mg, Ca, Na, Cu, Mn, Fe, and Zn using Atomic Absorbtion Spectrometer, and for P using spectrophotometer. Phosphorus content in different types of grains varied from 3.10 to 5.65 g kgE-1, potassium - from 4.37 to 6.10 g kgE-1, calcium - from 0.40 to 1.40 g kgE-1, magnesium - from 1.20 to 1.68 g kgE-1, manganese – from 14.50 to 55.54 g kgE-1, copper - from 3.35 to 6.01 g kgE-1, zinc - from 18.72 to 31.84 g kgE-1, iron - from 42.39 to 120.40 g kgE-1, and sodium - from 32.34 to 55.52 g kgE-1. The greatest difference was found between the content of manganese and iron in barley, wheat and oat grains, but small difference was between the mean content of P, K, Zn, Cu, Mg, and Na.

The aim of this study was to investigate the effect of industrially designed light-emitting diode (LED) lamp lighting on the nutritional quality of Brassicaceae microgreens. Red pak choi (Brassica rapa var. chinensis ‘Rubi F1 ’), tatsoi (Brassica rapa var. rosularis) and mustard (Brassica juncea L. ‘Red Lion’) were grown in a greenhouse (20±2/18±2 °C) during winter season, and the solar daily integral (DLI) was ~3.46±1.16 mol mE-2 dE-1. The light spectra of lamp consist of 8 violet (420-430), 16 blue (460-470 nm), 8 orange (610-615 nm), 3 red (620-630 nm), 56 red (660-670 nm), 8 white (contain blue (400-500 nm), green (500-600 nm) and red (600-700 nm)) LEDs. The treatments of ~150 and ~250 µmol m-2 sE-1 LED irradiance levels (LED 150 and LED 250) for 16 h dE-1 in comparison with high pressure sodium (HPS) lamps (~150 µmol mE-2 sE-1) as a control were performed. Photophysiological response to the artificial light varied among Brassicaceae species. Microgreens treated with LED 150 and LED 250 were significantly (P is less than or equal to 0.05) shorter and formed smaller hypocotyls. The photooxidative changes were evoked by both lighting treatments and led to higher phytochemical (phenols, ascorbic acid, flavonols, anthocyanins) and mineral element (Ca, K, Mg, Na, P, Fe, Zn) contents, and the DPPH and ABTS free radicals scavenging activities in all microgreens. Significantly lower content of nitrate was obtained with LED 150 treatment. Finally, LED lamps have the potential to be used as the main light source for growing high nutritional quality microgreens in greenhouses.