Nowadays excessively used corticosteroids in veterinary medicine induce steroid hepatopathy in dogs (Canis lupus familiaris). The objective of this study was to determine the possibility of the hepatoprotectants to decrease the corticosteroid-induced alteration in such dogs’ blood serum enzymes as alaninaminotransferase (ALAT), gammaglutamyltranferase (GGT), alkaline phosphatase (AP) and corticosteroid-induced thermostable alkaline phosphatase (cAP). The study took place in private veterinary clinics in Riga, Latvia, during 2013, with the permission of dogs’ owners. Twenty eight animals, which received corticosteroids due to present diagnosis, were divided into two groups. In the first group long-lasting corticosteroid methylprednisolone acetate injection was used once, while in the second group the hepatoprotectants were used after the injection of corticosteroids. It was discovered that after 14 and after 30 days of hepatoprotectants use, blood enzymes were significantly lower (p is less than 0.05) than in dogs that did not receive hepatoprotectants. In both groups the enzyme values did not reach the reference limits. The study is set to investigate further if and when the values reach the reference limits.

β-galactosidase (EC 3.2.1.23) is one of the widely used enzymes for lactose-free milk production and whey permeate treatment. Enzymes can be obtained from microorganisms, plants and animals. Nowadays, microorganisms are becoming an important source for production of commercially available enzymes, which are of great interest and offer several advantages such as easy handling and high production yield. The aim of this review was to summarize findings of research articles on the application of commercially available β-galactosidase preparates in dairy industry, to analyse and compare the most suitable β-galactosidase commercial preparates for lactose hydrolysis. The results showed that the main factor to choose an appropriate β-galactosidase for lactose hydrolysis was reaction condition. Enzymes from microorganisms contain a wide range of optimal pH from 4.0 (Penicillium simplicissimum and Aspergillus niger) to 8.5 (Bacillus subtilis). The greatest commercial potential has enzymes obtained from fungi (Aspergillus oryzae and Aspergillus niger) and yeasts (Kluyveromyces lactis and Kluyveromyces fragilis). Fungal origin enzymes are more suitable for the hydrolysis of lactose in acid whey due to its acidic pH but yeasts origin enzymes for milk and sweet whey. In the study, commercial preparates from different suppliers with the purpose to analyse their lactose hydrolysis potential and give more detailed characteristics of each preparate advantages and drawbacks were also summarized.

Corticosteroid therapy is often used on dogs (Canis lupus familiaris) to treat different kinds of diseases. One of the most common complications of corticosteroid use is steroid hepatopathy, a specific pathology only in dogs. The objective of this study was to determine whether and how significant the liver functional changes after one administration of different kinds of corticosteroids in standard dosages are. The study took place in private veterinary clinics in Riga, Latvia, during 2013 - 2014, with the permission of dogs’ owners. Twenty animals, which received corticosteroids due to present diagnosis, were divided into four groups. To reach the aim such corticosteroids as dexamethasone sodium phosphate, prednisolone acetate, methylprednisolone acetate and hydrocortisone aceponate were used in standard dosage one time to these dogs, respectively. Then, such blood serum enzymes as alaninaminotransferase (ALAT) and alkaline phosphatase (AP) were determined 24, 48 and 96 hours after the use of corticosteroids. It was discovered that the only one administration of dexamethasone sodium phosphate and methylprednisolone acetate in standard dosage can significantly increase (p is less than 0.05) ALAT and AP mean values in dogs. The corticosteroid prednisolone acetate was used once in standard dosage and hydrocortisone aceponate spray was used once and did not statistically significantly (p is greater than 0.05) change the values of alaninaminotransferase (ALAT) and alkaline phosphatase (AP) in dogs’ blood serum during this study.

The content of reducing sugars can be 30% higher if modified amylase is added to scald. Investigations were carried out with the rye-flour and fine flour scald. Photometrical method was used as a standard method for the determination of reducing sugars. Enzymes were inactvated using the advanced method. Results show, that the amount of reducing sugars is 1.8% lower in rye-flour and 2.1% lower in fine flour compare to the results obtained using the standard method.