The effect of dietary supplement with 17% high-amylose barley (Hordeum vulgare, HAB) 'Karmose' on boar taint was studied on 72 crossbred entire male pigs (Sus scrofa domestica) Swedish Yorkshire dams x Swedish Landrace sires). All pigs were fed the same commercial diet (12.4 MJ metabolised energy (ME) per kg, digestible crude protein (CP) 13.5%) twice a day until the average live weight (LW) in the pen reached 100 +- 11.8 kg. Then, 32 out of 72 pigs received an HAB diet. Half of these pigs received HAB for 14 days and the other half for 28 days. The pigs were slaughtered at an average LW of 122.3 +- 8.6 kg (age 167.2 +- 7.5 days). The HAB diet did not affect performance, carcass and meat quality characteristics, and puberty status, but reduced plasma skatole levels in the pigs (least squares means, 3.6 vs. 2.0 ng mlE-1, P = 0.037). However, this diet failed to reduce skatole, indole and androstenone levels in fat (P is greater than 0.10). Nevertheless, it is desirable to continue investigation of HAB to optimise the feeding procedure to improve efficiency of HAB effect on fat skatole levels. Additionally, validation of a greater amount of HAB in the diet should be performed.

Tryptophan is a precursor for the production of skatole and indole in the large intestine of pigs. Accumulation of these compounds in adipose tissue causes on objectionable odour in meat from entire male pigs. Potential market impact illustrates the importance in developing an accurate method of determining skatole and indole content in adipose tissue. In the present study, a method is described for determination of skatole and indole concentrations by liquid chromatography combined with mass spectrometry (LC-MS) using atmospheric pressure chemical ionization (APCI). The optimization of chromatographic and APCI conditions is reported. This method showed excellent linearity over the concentration range tested (from 10 to 1000 ng mlE-1 of liquefied fat for both skatole and indole).