Melon seeds have been recognized as a nutritional powerhouse, offering a range of essential vitamins and fatty acids that play a critical role in maintaining and enhancing our health. This study aims to analyse the fatty acid profile and water-soluble vitamins in melon seeds in order to highlight the importance of incorporating melon seeds into our diets, not only as a source of nutrients but also as a means of promoting overall health and well-being. It delves into the rich nutritional profile of melon seeds, underscoring their valuable contribution to a balanced diet. Water soluble vitamins C, PP and B1 were detected in melon seeds in this study. Regarding fatty acid profile, PUFA C18:2 was the most abundant (35.31%), followed by MUFA C18:1 (25.83%), although other unsaturated and saturated fatty acids were also determined. This comprehensive analysis of melon seeds’ nutritional benefits encourages further exploration of their potential in enhancing diet quality and supporting long-term health objectives.