Under open environmental conditions, Trihodermin and BioMikss were used for trial purposes to study lettuce (Lactuca sativa L.) and strawberry (Fragaria x ananassa Duch) yields in 2008. Experiments were carried out in the Region of Riga at the farm 'Pukulejas'. The lettuce cultivar 'May King' and the strawberry cultivar 'Senga Sengana' were both used in the trial. The purpose of the trial was to evaluate the use of biological products when applied on the above mentioned trial plants. In the field trial, both Trihodermin and BioMikss in dry powder form in peat mixture were applied for soil treatment 10 kg haE-1. Trihodermin contains cells of microscopic fungus Trichoderma harzianum 8-21 and Trichoderma viride 1-5. BioMikss is a mixture of microorganisms, containing cells of seven various bacteria (Azotobacter chroococcum 23, Polyangium cellulosum 5-t, Polyangium 56, Pseudomonas putida 48-t, Rhizobium meliloti 15, Streptomyces griseoviridis P-t and Streptomyces cellulosae D) and cells of two various fungus (Trichoderma harzianum 7-t and Trichoderma viride A-L). Three times during the growing period Trichodermin and BioMikss water solution 100 g kgE-1 was applied on the trial plants. When compared to non-treated plants, significantly increased yield was observed on BioMikss-treated lettuce. A significantly increased yield was also observed on BioMikss-treated strawberries. The results of the experiments showed that in open environment, both strawberry and lettuce plants treated with BioMikss showed resistance to the grey mould, caused by a parasitic fungus Botrytis cinerea. The results prove that biological products, by environmentally friendly means, revitalize soils, protect plants, and enhance growth, immunity and productive potential.

The research focuses on the study of physically-chemical and microbiological parameter changes in Latvian wild cranberries during convective drying. The research was accomplished on fresh Latvian wild cranberries. The following quality parameters were controlled during the experiments: moisture content (oven-dry method), content of vitamin C (LVS EN 14130:2003), content of polyphenol (HPLC), preparation of samples for microbiological testing (LVS EN ISO 7218:2007), enumeration of yeasts and moulds (ISO 21527-2:2008(E)), counting of lactic acid bacteria (LVS ISO 13721:1995), and total plate count (LVS EN ISO 4833:2003A). The research detected the following optimal convective drying parameters of wild cranberries: temperature – +50±1 deg C and drying time – 20.3 hours. The moisture content of dried berries equalled to 9.0±0.1%. The content of vitamin C decreased 1.9 times in wild cranberries dried at the temperature of +50±1 deg C compared with the content of vitamin C in non-dried cranberries and was 13.05 mg 100 gE-1 in dry matter. The results of current experiments show, that the content of such polyphenols as gallic, caffeic, and epicatechin acids decreased 9.70, 9.90, and 11.68 times, respectively, during the treatment at temperatures up to +50±1 deg C compared with the initial content of these compounds in non-dried berries. The drying temperature substantially influences the microflora development in cranberries. It is possible to decrease significantly the content of LAB, mould, and fungi in berries provided the drying temperature of the wild cranberries does not exceed +50±1 deg C, thus prolonging the ready product shelf-life.

The present study was undertaken to estimate enzymatically hydrolysed and non–hydrolysed wheat (Triticum aestivum) and rye (Secale cereale) bran microflora. Enzymatic hydrolysis was accomplished by α – amylase from Bacillus amyloliquefaciens and by Viscozyme L which contain a wide range of enzymes responsible for the breakdown of carbohydrates into simple sugars. Wheat and rye bran samples were collected from native mills, namely Stock Company (SC) ‘Rigas dzirnavnieks’ wheat bran with large particle size (WLSR), SC ‘Jelgavas dzirnavas’ rye bran with small particle size (RSSJ), SC ‘Dobeles dzirnavnieks’ wheat bran with small particle size (WSSD) and wheat bran with large particle size (WLSD). Gained results indicate that before enzymatic hydrolysis all of the bran samples showed similar microbiological contamination with total plate count (TPC), yeasts and lactic acid bacteria. Enzymatic hydrolysis of bran gives the possibility to partially eliminate the microbiological contamination with TPC, yeasts and lactic acid bacteria. The amount of microorganisms after enzymatic hydrolysis (before storage) were decreased and ranged from 5.26 ± 0.04 to 5.45 ± 0.01 log CFU gE-1, from 4.81 ± 0.01 to 5.60 ± 0.05 log CFU gE-1, and from 4.09 ± 0.01 to 5.10 ± 0.05 log CFU gE-1, respectively. After eight weeks of storage (temperature – 20 ± 1 °C, relative humidity – 40 ± 1%) enumeration of microorganisms showed significant decrease of colony–forming units in all bran samples. The amount of TPC, yeasts and lactic acid bacteria in the control bran samples fluctuated in a range from 4.84 ± 0.04 to 5.49 ± 0.05 log CFU gE-1, from 4.86 ± 0.03 to 5.25 ± 0.03 log CFU g-1, 3.53 ± 0.03 to 4.21 ± 0.02 log CFU gE-1 respectively.

The objective of the present study was to investigate the microbiological content of cows’ milk in Latvia’s organic farms with a purpose to detect potential microbiological threats in milk. Samples were collected in December 2011 at 12 biological dairy farms of Latvia. Raw milk samples (N=155) obtained from cow composite milk were studied. The total mesophilic aerobic and facultative anaerobic microorganisms (MAFAM), the presence of coliforms and coagulase-positive staphylococci, count of yeasts and moulds were analysed using standard methods. Of the sampled cows 50% had a low somatic cell count (SCC) (LESS THAN 200,000 cells mLE-1), 23% - high, but 27% had a very high SCC (greater than 500,000 cells mLE-1). The mean value of MAFAM in the samples with low, high and very high SCC was 4.7, 5.0 and 5.0 log10 colony forming units (cfu) mLE-1, respectively. The yeasts were present in 57% of milk samples with the mean concentration of 3.1 log10 cfu mLE-1. Moulds were found in 27% of all milk samples; their mean concentration was 4.4 log10 cfu mLE-1. Identified mould strains belonged to genera Absidia, Aspergillus, Geotrichum, Mucor and Penicillium. In cases of subclinical mastitis and latent mammary infection the most distributed mastitis pathogens were Staphylococcus aureus, Micrococcus kristinae, Bacillus cereus and coagulase negative staphylococci.

Grain legumes are important crops for the diversification of European farming system. In Latvia, the areas of faba beans (Vicia faba) and field peas (Pisum sativum) are increasing and legumes that have not been previously cultivated are sown. Globally, the important causal agent of legume diseases belongs to the genus Botrytis. Botrytis spp. cause significant losses in faba beans and infect a wide range of legume crops. Currently, the composition, pathogenicity and biological characteristics of Botrytis species in legumes in Latvia are not known. The aim of this study was to summarize the information about Botrytis spp. on legumes, using monographic method. The researches carried out all over the world show a high genetical diversity of Botrytis cinerea. Non-host specific B. cinerea has been found on 586 plant genera, including many legume crops. B. cinerea causes gray mold on leaves and pods of field peas, gray mold on pods of faba beans, and chocolate spot of faba bean leaves. B. fabae can infect plants from Fabaceae family. B. fabiopsis is known as a pathogen of faba beans. B. fabiopsis has been observed in Latvia, but no information is available on its presence in Europe. B. pseudocinerea has been found on beans, peas, and clover (Trifolium). Some other Botrytis spp. have been detected on legumes; however, their occurrence and importance are not clear. Summary of existing knowledge about Botrytis spp. on legumes is necessary to continue investigations about the diversity and economic importance of Botrytis spp. in legumes.

A total of 120 food samples from 6 traditional and 3 non-traditional (Oriental) public catering establishments in Latvia were taken. Preliminary all food samples were divided in three food groups (categories) depending on the components of the products and processing methods. Ready-to-eat foods (RTE) with high level of total amount of microorganisms (aerobic colony count-ACC) and total coliforms (TC), as well as appropriate infringements of hygienic requirements in public catering establishments which had an impact on microbial contamination were clarified. The reasons for microbiological contamination of RTE and the growth of the microorganisms were analyzed in dependence on food category group, the activity of hydrogen ions (pH) in food, food temperature at the sampling point, their processing mode and composition. Different indicator microorganisms including Staphylococcus, Enterobacter, Escherichia, Bacillus, yeasts, moulds and etc. were identified. The comparison between the temperature and pH and the level of microbiological contamination of the samples of food and hygienic practice in the catering establishments were clarified.

The effect of some ingredients on stability of butter during its storage time has been investigated in order to better evaluate the functionality of confectionery. The research established that butter does not contain enough free fatty acids, derived as a result of hydrolysis, to decrease the number of mould and group of Enterobacteriaceae, but they hinder the growth of yeast and aerobic mesophilic and facultative anaerobic microorganisms. The quality of the products can be provided and propagation of microorganisms can be hindered by the comounds that are not traditional preservatives, such as vanilin and glucose syrop. A 10% glucose syrup additive worked as the facilitator of the development of microorganisms because it increased growth of aerobic mesophilic and facultative anaerobic microorganisms, 50% of glucose syrup additive worked as the procrastinator of the development of microorganisms.