The agronomic role of legumes in cropping systems is well understood with the respect to N2 fixation, mechanisms of precrop effects, and environmental impacts. The combined inoculation and tripartite symbiosis between leguminous plants, Rhizobium spp. and vesicular-arbuscular mycorrhizal fungi has been the subject of intensive research. Less attention has been paid to their effects on subsequent crops. Pot experiments were carried out in the greenhouse of Latvia University of Agriculture in 2015 and 2016. Soil used for this experiment was taken from the previous trial, where broad beans (Vicia faba var. major Harz.) were grown. Bean seeds, depending on the variant, were inoculated with rhizobia bacteria or mycorrhiza fungi, or the mixture of both microorganisms. Onions were grown as a subsequent crop. During the experiment, fresh and dry weight of onion leaves was determined. Onion root mycorrhizal colonization frequency and arbuscule abundance in the onion root system were determined. The activity of soil microorganisms was determined by soil respiration intensity. Results showed that the use of microsymbionts increased the subsequent onion leaf harvest in 2014 by 2.3% and in 2015 by 9.5%. In 2015 the highest increase of onion leaf yield was detected in variants were rhizobia strain RP023 was used. Rhizobia strain RV407 gave a positive effect only in combination with mycorrhiza fungi. Precrop treatment with mineral nitrogen fertilizer increased the onion yield in both years – by 12.4 and 14.3%, respectively. In all treatments, a decrease in dry matter content was observed. The highest incidence of mycorrhiza fungi structures was detected under mycorrhiza treatment. Tripartite symbiosis promoted the soil respiration rate.

Healthy seeds are essential for the optimal plant population and yield, but seed-borne pathogens, such as Fusarium spp., may reduce seed germination, quality and cause damping-off of the seedlings. Fusarium graminearum is a dominant pathogen of cereal crops and can cause significant losses of grain yield and quality. It is important to evaluate the role of alternative inoculum source in crop rotation. The aim of this study was to assess the impact of F. graminearum infection on different plant seed germination and seed infestation. The research was conducted at the Institute of Agriculture, Lithuanian Research Centre for Agriculture and Forestry, in 2017. Visually healthy seeds of bean (Vicia faba L.), pea (Pisum sativum L.), lupine (Lupinus angustifolius L.), soybean (Glycine max. (L.) Merr.), lucerne (Medicago sativa L.), white (Trifolium repens L.) and red (Trifolium pratense L.) clover were inoculated with 10 mL of F. graminearum suspension, adjusted to 1×106 conidia per mL. Seed infection was counted 2 and 6 days after inoculation (DAI), seed germination energy and reduction rate – after 3 DAI and germination index – 6 DAI. Results showed that all inoculated seeds were covered with typical to F. graminearum red-purple mycelium. The results of inoculated seeds with F. graminearum showed red-purple mycelium growth on the seeds (infection from 21.25 up to 100%). The results showed that germination energy decreased on pea (2.56%) and lupine (7.79%) seeds. Our results suggest that various plant seeds differently react to F. graminearum infection. The highest infection of F. graminearum was obtained on pea, lupine seeds and the least on red clover.