This study was performed to detect morphological changes of the gastric mucosa according to the prevalence of helicobacters in the gastric mucosa of domestic dogs (Canis familiaris) from the Small animal clinic of Faculty of Veterinary Medicine, Latvia within 2008 year period. Mucosal samples were taken from several places of cardiac, fundic and pyloric gland region of the stomach to detect helicobacters with light histological examination. Seventeen dogs of eighteen sampled animals showed presence of tightly spiraled helicobacters. Compared to gastric regions our study showed significantly higher prevalence of helicobacters in the cardiac and fundic gland region than in the less effected pyloric gland region of the stomach. Histological examination also showed the depth of location of helicobacters in gastric pits and deeper glandular epithelium of the gastric mucosa in different regions of the stomach. In the pyloric gland region helicobacters are located deeper in the gastric pits and glandular epithelium than in the gastric mucosa of cardiac and fundic gland region Morphological changes of gastric mucosa was divided into 4 groups: 1) mucosa without morphological changes, 2) mucosal hyperemia, 3) mucosal erosions, and 4) mucosal polips. Morphological changes especially mucosal polips are significantly more in the pyloric gland region than in the cardiac and fundic gland region. The amount of morphological changes in the helicobacter positive and negative samples were not significantly different in the cardiac and fundic gland region, but in the pyloric gland region morphological changes of the gastric mucosa are significantly more in the helicobacter positive samples than in the helicobacter negative samples.

A study was conducted to determine efficiency of an organic acids and phytogenic additives on pigs' growth processes and digestive tract microflora. The feed of the control group (C) did not contain the additives. The feed of the organic acid group (A) contained 6 kg of organic acids additive per tonne feed for pigs after weaning, 4 kg per tonne feed for grower pigs, and 3 kg per tonne feed for finished pigs. The feed of the phytogenic additive group (F) contained 0.5% of phytogenic additive per tonne feed for pigs after weaning, and 0.2% per tonne feed for grower and finished pigs. The feed of organic acids plus phytogenic additives group (A+F) contained 6 kg of organic acids additive per tonne feed for pigs after weaning, 4 kg per tonne feed for grower pigs, and 3 kg per tonne feed for finished pigs 0.5% of phytogenic additive per tonne feed for pigs after weaning, and 0.2% per tonne feed for grower and finished pigs. Compared to groups pigs from the group (F) had by 9.7%, group (A+F) - by 8.3 % and from group (A) had by 3.2% higher mass. The bests results of feed conversion were found in the group (A+F) - 2.84 kg, which was by 8.5% less than in the group (C), whereas the group (F) had by 5.1% and group (A) - by 6.7% better results than the control group. The results of Duodenum and Rectum microflora analyses showed that use of organic acid and phytogenic additives reduced mould yeast Staphylococcus sp. colony formed units (CFU gE-1).

A study was conducted to determine efficiency of an organic acids additive on pigs metabolic processes and diggestive tract microflora. The pigs of control group were fed without an organic acids additive. The feed of the trial group piglets contained 6 kg of organic acids additive per tonne feed, for starter pigs - 4 kg per tonne feed, for finished pigs - 3 kg per tonne feed. The study indicated that at the age of 130 days, pig mass in the trial group was 54.08 kg an average, but in the control group - 51.06 kg, which showed that pigs from the trial group had by 6% higher average mass than in the control group (p is less than 0.005). At the age of 160 days, pig mass in the trial group was an average 94.3 kg, in the control group - 90.63 kg; pigs from the trial group had by 4% higher mass than in the control group (p is less than 0.005). Feed consumption for the trial group was by 1% less than in the control group. Average daily gain for the trial group was 0.663 kg, which was by 6% more than for the control group pigs (p is less than 0.005). Feed conversion in the trial group was 3.06 kg, but in the control group - 3.275 kg, i.e. 6.4% higher than in the trial group. Analyses of Jejunum microflora showed that use of acidifier additive reduced Mould colony formed units - CFU amount in 1 g of the trial group sample by 6%. Staphylococcus species amount in the trial group was 500 CFU in 1 g of sample, but in the control group - 40000 CFU, i.e. 80 times more. In the trial group, Lactic acid bacteria CFU amount in 1 g of sample was by 6% higher than in the control group. The results of Rectum microflora analyses showed that Staphylococcus species amount in the trial group was 12000 CFU in 1 g of sample, but in the control group it was by 34% higher.

A study was conducted to determine efficiency of a phytoadditive on pig growth processes and digestive tract microflora. The pigs of control group were fed without the phytoadditive. The feed of the trial group piglets contained 0.5% of the phytoadditive per tonne feed, for starter pigs and finished pigs - 0.2% per tonne feed. The study indicated that at the age of 170 days, pig mass in the trial group was 111.67+-1.22 kg on average, but in the control group - 101.79+-0.81 kg, which showed that pigs from the trial group had by 9.7% higher average mass than in the control group (p is less than 0.05). Average daily gain for the trial group was 0.777+-0.009 kg, which was by 12 % more than for the control group pigs (p is less than 0.05). Feed conversion in the trial group was 2.928 kg, but in the control group - 3.129 kg, which was by 6.4% higher than in the trial group. Gastric microflora analyses showed that use of phytoadditive reduced mould colony forming units (CFU) amount in the trial group decreased by 24 times. Duodenum microflora analyses showed that use of phytoadditive reduced mould CFU amount by 25%, yeast CFU amount by 34%, Escherichia coli mesophilic and termophilic forms CFU by 16.3% compared to the control. A lactic acid bacterium CFU in the trial group was 2.5 times higher compared to the control. Rectum microflora analyses showed that use of phytoadditive reduced mould CFU amount by 31.6%, yeast CFU amount - by 62%, Escherichia coli mesophilic and termophilic forms by 57 % and 15.6 % respectively. Lactic acid bacteria CFU amount in 1 g of sample in the trial group increased by 5.1 times.

The aim of this study was to determine the influence of Jerusalem artichoke flour feeding on the general health status of calves (Bos Taurus), the animal live weight gain and digestive canal morphological development in the first four months of life, as well as to find out the optimal of three inulin doses. Jerusalem artichoke (Helianthus tuberosus) concentrate produced in Latvia contains the prebiotic – inulin. The study was performed on four groups of animals - a control group of 10 animals and three prebiotic groups, 10 animals in each group. All groups were fed the whole milk, but the PreG6 group animals were fed daily with 6 grams of prebiotic, calves PreG12 group were fed with 12g of prebiotic, but each calf in PreG24 group – 24g of prebiotic per day. CoG control animals did not receive feed supplement. Faecal mass consistence of PreG6, PreG12 groups of animals was more stable within the whole research period than CoG. We found that the control group animal weight gain on the 56th research day is statistically significantly (p is less than 0.05) lower than of those calves that received inulin as a food supplement. The highest growth rates have shown PreG12 and PreG24 group of animals, significantly (p is less than 0.05) being ahead of PreG6 group of calves, the highest average daily live weight gain was observed in PreG24 group (0.95 ± 0.093 kg).