Future perspective of the research is the production of fuel pellets from energy plant (Phalaris arundinacea L., Festuca arundinacea, etc.) biomass, because it can be better burnt in granule burners and is more environmentally friendly, if compared to the fossil mineral resources (coal, oil, gas), moreover it has low moisture content (70 – 90 g kgE-1) and correspondingly it produces higher combustion energy. The research covered preparation of various content tablets from reed canary grass (Phalaris arundinacea L.) variety ’Marathon’ (N fertilizer rate on the N-90 kg haE-1), energy wood - osier (Salix viminalis L) and poplar (Populus tremula L.) with N fertilizer norms N-0 and N-120 kg haE-1, and afterwards research of the combustion ability of and ash content in these tablets. Combustion ability of reed canary grass (Phalaris arundinacea L.) variety ‘Marathon’ reached 17.48 MJ kgE-1. The highest average combustion ability with different pellet content was found for the fast-growing poplar both with doses of N fertilizer - 18.55 MJ kgE-1 and without N fertilizer - 18.49 MJ kgE-1. Optimum content of various component pellets for biomass was a mixture of components 1/3 (reed canary grass/osier or poplar). The lowest indicators in respect to the ash content were observed for osier (Salix viminalis L.) - 27.9 g kgE-1. The best ash content indicators for a mixture of granular composition was in a mixture of components one-fourth of the reed canary grass with three parts of osier - 34.3 g kgE-1 or with poplar - 41.8 g kgE-1.

This paper discusses some aspects of introducing a new agricultural crop in Latvia – truffles Tuber spp. Truffles are mycorrhiza–forming mushrooms with edible fruit bodies developing in the upper layer of soil. In Latvia, there is a growing interest among farmers to start cultivate truffles. From several cultivated truffle species, the Burgundy truffle Tuber aestivum syn. T. uncinatum is the most suitable to cultivate in Latvia. There are both autochthonous and introduced tree species in Latvia, which are suitable for the Burgundy truffle cultivation and it is suggested to use Quercus robur, Corylus avellana, Tilia cordata and Fagus sylvatica. In order to find the regions in Latvia with the highest potential for successful Burgundy truffle cultivation, climate and soil characteristics had been co-analysed. By superimposing soil and climate maps of Latvia, the most suitable regions for truffle cultivation are situated in the central and southern part of the country. Considering the variability in soil composition even within regions in Latvia, it is thereafter also of great importance to investigate site-specific soil characteristics in order to find the most favourable grounds for truffle cultivation. Other truffle species which might be suitable for cultivation in the future are also discussed, as well as some general recommendations in establishing truffle orchards.

The aim of the present research was to evaluate the affect of genetic and environmental factors on the variability of the goat milk yield, fat and protein content in goat milk in Latvia. Data of 6067 lactation records from 2400 goats of six different breeds were analysed in the period of 2001 to 2010. The highest milk yield (662.7±14.58 kg) was obtained in 2002, the highest fat content (40.7±0.05 g kgE-1) – in 2004, but highest protein content (32.7±0.19 g kgE-1) – in 2008 (p is less than 0.05). Basically there are two goat breeds in Latvia: Latvian goats and Saanen goats. In the research, 3261 Latvian and 2032 Saanen goats in closed lactations were analysed. It was found that Saanen goats gave the highest milk yield (579.3±5.01 kg), but Alpine goats – the highest fat and protein content (respectively 41.7±0.63 and 32.3±0.30 g kgE-1; p is less than 0.05). It was observed that most of all the goats kidded in winter (2379) and spring (3378). The highest milk yield (583.8±7.39 kg) was determined for goats kidded in winter season, but the highest fat content (41.8±0.06) and protein content (32.3±0.03) – for goats kidded in summer season. The average milk yield in the first lactation (1636) was significantly lower than in the third lactation (578.0±8.34; p is less than 0.05) when the goats produced the highest milk yield in the research.

Horseradish (Armoracia rusticana L.) is a perennial plant, with a particularly pungent flavour and significant antioxidant properties. The aim of current research was to determine the total phenol content and antioxidant properties of horseradish depending on genotype and harvest time. For experiments nine genotypes of horseradish roots collected at different times were investigated. Fresh plant material was extracted with ethanol/water solution (80:20 v/v). Total phenols content (TPC) of plant extracts was determined according to the Folin-Ciocalteu spectrophotometric method and results were expressed as gallic acid equivalents (GAE). Antioxidant activity of the extracts was measured on the basis of DPPH˙ free radical scavenging activity and the final results were expressed as inhibition of 2,2-diphenyl-1- picrylhydraziyl (DPPH˙) in percent (I, %). Total phenols content varied among analysed types of horseradish. The highest TPC was detected in horseradish root genotype 280 harvested in September and it also demonstrated the highest DPPH˙ radical scavenging activity, whereas the lowest TPC was detected in horseradish root genotype 26B also harvested in September. TPC and DPPH˙ scavenging antioxidant activity were also significantly influenced by harvest time. Positive correlation was found between antiradical activity and the total phenols content in horseradish roots harvested in September. In further experiments, use of horseradish as natural antioxidants in different food matrixes should be studied.

Carrots (Daucus carota L.) are a globally important vegetable crop providing a source of important nutritional compounds through their carotenoid content whilst adding flavour and texture to many diets across the world. The current research focuses on the evaluation of sugars, vitamin C and dietary fibre content in ‘Nante’ hybrid carrots. The research was accomplished on fresh in Latvia cultivated carrots harvested in Zemgale region in the first part of October 2011 and immediately used for experiments. Late-bearing hybrids of ‘Nante’ carrots were used for analysis: Nante/ Berlikum, Nante/Maestro, Nante/Forto, Nante/Bolero, and Nante/Champion. The major sugars (fructose, glucose and sucrose) were determined by applying the method of high performance liquid chromatography, vitamin C - by titration with 0.05 M iodine solution, and dietary fibre - using standard method No 985.29. In the present experiments it was found that there are significant differences in the sugar, vitamin C and dietary fibre content between different carrot hybrids. The highest content of total sugars was found in Nante/Maestro and Nante/Champion 7.99 g 100 gE-1 and 7.57 g 100 gE-1 hybrids in fresh weight, respectively. The lowest total sugars content was in Nante/Berlikum hybrid − 4.03 g 100 gE-1. Vitamin C content in carrot hybrid Nante/Maestro was the highest 17.61±0.17 mg 100 gE-1, but in hybrid Nante/Champion - the lowest 8.39±0.17 mg 100 gE-1 of fresh weight. The dietary fibre content in analysed carrot samples ranged from 34.25±0.47 g 100 gE-1 in Nante/Maestro to 25.78±1.54 g 100 gE-1 in Nante/Champion hybrids.

Cereal and whole grain products are an important source of dietary fiber in the human diet. Since the milling process highly influences the proportions of the different cell types in the bran, it is expected that brans originating from different genetic/agricultural backgrounds and produced by different processes have different chemical composition. Wheat (Triticum aestivum) bran has not been studied in Latvia, therefore the aim of the present study was to analyze physical – chemical characteristics of wheat bran from Latvia’s industrial mills. Four bran samples were collected from two industrial mills: Stock Company ‘Dobeles dzirnavnieks’ and SC ‘Rigas dzirnavnieks’. All experiments were performed at the Food Technology of Latvia University of Agriculture in May 2011-February 2012. The particle size distribution of the bran samples was determined by sieving. The content of titratable acidity (TA) was detected by titration with 0.1 M NaOH till pH 8.5 was stable for 1 minute. Wheat bran colour was analysed by Hunter Lab colour measurement instrument using ColorTec colorimeter PCM/PSM in CIE L * a * b* system. Moisture content was analyzed using standard ICC 110/1 method by sample drying for 2 h at 150 °C. Results showed that there were significant differences between varieties (p0.05) for titratable acidity and for particle size, but no significant differences (p0.05) were found between the varieties in moisture, pH and colour. TA varied from 6.40±0.71 to 12.05±0.21°, moisture content- from 10.01±0.51% to 11.61±0.47%, pH–from 6.31±0.61 to 6.80±0.05, but colour of bran between the samples did not significantly differ.

Barley (Hordeum vulgare L.) grain is the main raw material in brewing industry, because beer taste and production process depend on barley grain qualitative indices. One of the barley grain’s qualitative indices is grain sprouting activity. There have been numerous researches that showed the effects of selenium (Se) on hull-less barley sprouting activity and positive influence on biologically active substances and high vitamins concentration in grain (Dūma et al., 2002), but hull-less barley is not widely used. The aim of this study was to explore the influence of different selenium concentrations on the barley grains sprouting activity and content of total phenols. Barley with grain viability of 92% have been soaked in diverse solutions (Se concentration 0.5 mg LE-1 – 10 mg LE-1) for 12 hours, then the grain sprouted at the temperature of +18±2 °C for 5 days and dried in the oven for 48 hours at a temperature of +50 °C. After sprouting, the sprouting activity of the grain was determined, but total phenols amount was determined after grain desiccation. The obtained results showed that the increase in selenium concentration in a solution increased barley grain sprouting activity from 16.3% (0.5 mg LE-1) to 27.9% (10 mg LE-1), but the amount of total phenols decreased.

Venison is well known as a traditional meat type in Europe, and it is lower in calories, cholesterol and fat content than common cuts of beef, pork or lamb. The aim of the current research was to determine physico-chemical parameters such protein, fat, pH, moisture content as well microbiological quality of pickled venison during storage. Beef as a control was analyzed for comparison of obtained results. The meat (2×3×2 cm) pieces were pickled in vinegar marinade (composition: tomato sauce, mayonnaise, vinegar, lemon, onion, parsley, paprika, basil, black pepper, rosemary, salt) at 4±2 °C temperature for 48±1 h. The marinated meat was placed in polypropylene trays and hermetically sealed with high barrier polymer film under modified atmosphere (C02 40%+N2 60%) without and with iron-based oxygen scavenger sachets (Mitsubishi Gas Chemical Europe Ageless®). As a control, packaging in air ambiance packed pickled products was used. During storage time, the moisture and protein values significantly (p is less than 0.05) decreased and pH, fat content and colony forming units significantly (p is less than 0.05) increased in the pickled venison samples of all packages. Slower changes in pH of pickled venison and in the protein and moisture content of marinated beef were observed in modified atmosphere with oxygen scavenger during storage.

The survival of inoculated in a cold-smoked sausages Listeria monocytogenes wild strains was studied. The sausages were prepared with and without starter cultures. The survival limits of L. monocytogenes and lactic acid bacteria (LAB) were determined as colony forming units per gram (cfu gE-1) depending on water activity (aw) and pH on 0, 1st, 3rd, 5th, 7th, 14th and 21st days of maturation. The decreasing water activity conditioned by moisture (weight) loss during ripening and pH decrease ensured negative polynomial growth rate of inoculated L. monocytogenes - 0.27 lg (cfu gE-1) each day of ripening time, and - 0.65 lg (cfu gE-1) on the first 7 days of maturation. A significant Pearson’s correlation (p is less than 0.01) was established between decreased values of L. monocytogenes count, aw, salt concentration and LAB growth in sausages during the ripening period of 21 days. The main parameters, maintained negative exponential growth rate of L. monocytogenes in cold smoked sausages, are aw value decrease and LAB (starter culture), which stopped L. monocytogenes growth at the beginning of cold-smoked sausage maturation. If fermentation process went technically and hygienically correctly, the fermented (cold-smoked) sausages could be one of the safest meat products, because in real practice a low level contamination has been seen. The remaining count of L. monocytogenes in cold-smoked sausage depends on the possible initial contamination level and could exceed the European Union regulation value 2.0 lg (cfu gE-1) for ready-to-eat products when contamination at first is more than lg 5.0.

The objective of the present study was to investigate the microbiological content of cows’ milk in Latvia’s organic farms with a purpose to detect potential microbiological threats in milk. Samples were collected in December 2011 at 12 biological dairy farms of Latvia. Raw milk samples (N=155) obtained from cow composite milk were studied. The total mesophilic aerobic and facultative anaerobic microorganisms (MAFAM), the presence of coliforms and coagulase-positive staphylococci, count of yeasts and moulds were analysed using standard methods. Of the sampled cows 50% had a low somatic cell count (SCC) (LESS THAN 200,000 cells mLE-1), 23% - high, but 27% had a very high SCC (greater than 500,000 cells mLE-1). The mean value of MAFAM in the samples with low, high and very high SCC was 4.7, 5.0 and 5.0 log10 colony forming units (cfu) mLE-1, respectively. The yeasts were present in 57% of milk samples with the mean concentration of 3.1 log10 cfu mLE-1. Moulds were found in 27% of all milk samples; their mean concentration was 4.4 log10 cfu mLE-1. Identified mould strains belonged to genera Absidia, Aspergillus, Geotrichum, Mucor and Penicillium. In cases of subclinical mastitis and latent mammary infection the most distributed mastitis pathogens were Staphylococcus aureus, Micrococcus kristinae, Bacillus cereus and coagulase negative staphylococci.