This study was carried out to determine the levels of retinol, α-tocopherol, cholecalciferol and reproductive hormones (follicle stimulating hormone - FSH, luteinizing hormone - LH, progesterone, estradiol) in the female rats administrated Allium schoenoprasum L. ethanol extract (ASLEE) and acrylamide. The study was performed on thirty-two Wistar albino female rats (200-220 grams). The rats were divided into 4 groups with an equal number. Serum LH values were higher in the ASLEE group compared to the other groups. Control group: No treatment was performed. Acrylamide group: Acrylamide was administrated by gastric gavage at a dose of 25 mg / kg daily. ASLEE group: ASLEE was administrated by gastric gavage at a dose of 200 mg / kg daily. Acrylamide + ASLEE group: Acrylamide was administrated by gastric gavage at a dose of 25 mg / kg daily. Then ASLEE was administered by gastric gavage at a dose of 200 mg / kg per day. Serum FSH and LH values were significantly lower in the acrylamide group compared to the other groups. Serum LH values in the acrylamide + ASLEE group were significantly restored compared to the acrylamide group. Serum estradiol values were partially lower in the acrylamide group compared to other groups, but there was no significant difference between the groups. Serum progesterone values in the acrylamide group were significantly lower than the control group. Serum progesterone values were higher in the acrylamide + ASLEE group compared to the acrylamide group. As a result, the levels of retinol, α-tocopherol, cholecalciferol and reproductive hormones in ASLEE were determined in this study. In addition, when ASLEE was applied with acrylamide, the rate of change in the relevant parameters was determined.