Bu çalışmanın amacı, yarı kurak iklim bölgesinde tesis edilen ve farklı kök sistemlerine sahip olan üzümsü meyvelerinin toprak sıkışması üzerine etkilerinin araştırmaktır. Bu amaçla ahududu, böğürtlen ve gojiberi yetiştiriciliği yapılan bahçede toprak sıkışmasını değerlendirmek için hacim ağırlığı, penetrasyon direnci ve kesme direnci ölçümleri yapılmıştır. Elde edilen bulgulara göre, bahçedeki henüz üç yaşındaki bitkilerin toprakların hacim ağırlığı üzerine bir etkisi bulunmamıştır. Ancak kök sistemleri birbirinden farklı olan bu bitkilerden böğürtlen ve gojiberinin toprakların penetrasyon direncine etkisi aynı iken ahududunun yetiştiği toprağın penetrasyon direnci diğer ikisinden istatiksel bakımdan farklı ve daha düşük bulunmuştur. Her üç bitkinin yetiştiği toprağın kesme direncinin birbirinden istatiksel bakımdan farklı olduğu, yetiştikleri toprakların kesme direnci değerleri bakımından bitkilerin böğürtlen

In the present study, totally 49 samples, which showed the symptoms of leaf and shoot blight and cankers with brown discoloration of necrotic tissues on mature branches, were collected from 22 districts and areas of Konya Province between 2017 and 2019. Presence rate of E. amylovora in collected samples, showing symptoms of the disease, from the province was determined to be 40% for blackberry and raspberry and 33% rosehip for rosehip in three years. Bacteria consistently isolated from the diseased tissues were identified on the basis of biochemical, physiological, and molecular tests, comparing with a reference strain of E. amylovora, isolated from blackberry (Kbb 371). Twenty seven representative bacterial strains were gram-negative, rod-shaped, mucoid, fermentative, positive for levan formation and acetoin production, no growth at 36°C, positive for gelatin hydrolysis, and negative for esculin hydrolysis, indole, urease, catalase, oxidase, arginine dehydrolase, reduction of nitrate, acid production from lactose, and inositol. All strains induced a hypersensitive response in tobacco (Nicotiana tobacum cv. White Burley) 24 h after inoculation with a 108 CFU ml-1 bacterial suspension in sterile distilled water. The strains were identified as E. amylovora using the species-specific primers set A/B (1), which amplified a 1-kb DNA fragment in PCR, and the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) method. In order to fulfill the Koch postulates, pathogenicity test was confirmed by injecting bacterial suspensions of 108 CFU ml-1 in sterile distilled water into the shoot tips of 3-year-old blackberry R. fruticosus cv. Chester, raspberry R. idaeus cv. Heritage and rosehip R. canina. All tests were repeated three times. The bacterium was re-isolated from inoculated plants and identified as E. amylovora. Phytosanitary measures are needed to prevent any further spread of the bacterium as potential inoculum sources to new blackberry, raspberry and rosehip growing areas.

In the present study, totally 49 samples, which showed the symptoms of leaf and shoot blight and cankers with brown discoloration of necrotic tissues on mature branches, were collected from 22 districts and areas of Konya Province between 2017 and 2019. Presence rate of E. amylovora in collected samples, showing symptoms of the disease, from the province was determined to be 40% for blackberry and raspberry and 33% rosehip for rosehip in three years. Bacteria consistently isolated from the diseased tissues were identified on the basis of biochemical, physiological, and molecular tests, comparing with a reference strain of E. amylovora, isolated from blackberry (Kbb 371). Twenty seven representative bacterial strains were gram-negative, rod-shaped, mucoid, fermentative, positive for levan formation and acetoin production, no growth at 36°C, positive for gelatin hydrolysis, and negative for esculin hydrolysis, indole, urease, catalase, oxidase, arginine dehydrolase, reduction of nitrate, acid production from lactose, and inositol. All strains induced a hypersensitive response in tobacco (Nicotiana tobacum cv. White Burley) 24 h after inoculation with a 108 CFU ml-1 bacterial suspension in sterile distilled water. The strains were identified as E. amylovora using the species-specific primers set A/B (1), which amplified a 1-kb DNA fragment in PCR, and the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) method. In order to fulfill the Koch postulates, pathogenicity test was confirmed by injecting bacterial suspensions of 108 CFU ml-1 in sterile distilled water into the shoot tips of 3-year-old blackberry R. fruticosus cv. Chester, raspberry R. idaeus cv. Heritage and rosehip R. canina. All tests were repeated three times. The bacterium was re-isolated from inoculated plants and identified as E. amylovora. Phytosanitary measures are needed to prevent any further spread of the bacterium as potential inoculum sources to new blackberry, raspberry and rosehip growing areas.

Bu çalışmanın amacı, yarı kurak iklim bölgesinde tesis edilen ve farklı kök sistemlerine sahip olan üzümsü meyvelerinin toprak sıkışması üzerine etkilerinin araştırmaktır. Bu amaçla ahududu, böğürtlen ve gojiberi yetiştiriciliği yapılan bahçede toprak sıkışmasını değerlendirmek için hacim ağırlığı, penetrasyon direnci ve kesme direnci ölçümleri yapılmıştır. Elde edilen bulgulara göre, bahçedeki henüz üç yaşındaki bitkilerin toprakların hacim ağırlığı üzerine bir etkisi bulunmamıştır. Ancak kök sistemleri birbirinden farklı olan bu bitkilerden böğürtlen ve gojiberinin toprakların penetrasyon direncine etkisi aynı iken ahududunun yetiştiği toprağın penetrasyon direnci diğer ikisinden istatiksel bakımdan farklı ve daha düşük bulunmuştur. Her üç bitkinin yetiştiği toprağın kesme direncinin birbirinden istatiksel bakımdan farklı olduğu, yetiştikleri toprakların kesme direnci değerleri bakımından bitkilerin böğürtlen