The aim of the study was to find the diversity index of flora, investigation of species at pond area and biomass calculation of economic plants at Chashma Barrage, Pakistan. The study area consisted of river Bella, pond area and eastern edge of river bed near Ali Wali Ghandi village at Chashma Barrage. The seasonal variation in the abundance of floral species was also related with the increase in temperature. Cyperus deformus, Phragmites kerka, Saccharum spontaneum were most abundant in the river ‘Bella’ habitat. Persicassia amphibian, Cyperus deformus and Polygonum royleanum were abundant in Aliwali Ghandi habitat. Aquatic plants of families Potamogetonaceae, Nymphaeaceae, Najadaceae and Hydrocharitaceae were most common in pond area . The ecotones were wide and variable between the aquatic biotopes and the river bed biotopes. On the Aliwali Ghandi site the moist soil with puddles and small channels of water were found and Cyperus deformus biotope prevailed. In the river ‘Bella’ site Phragmites, Sacharum was the biotope and in water Hydrilla biotope was recorded. An increase in biomass of Phragmites, Sacharum and Typha was recorded with the passage of time. The species of the area have significant importance in socio economics of the local community of Chashma Barrage.

The roles of protein in bodybuilding and the regulation of biological processes are important in sustaining life. A large amount of protein is required by both humans and animals and this cannot be supplied by only conventional sources. This is because of the rapid increase in world population. The present sources of protein will not meet global protein demand in years to come. Scientists explore the production of single-cell protein (SCP), as an alternative source of protein, through the utilization of wastes and low-value materials. SCP can supply high-quality protein containing both essential and non-essential amino acids that can be utilized by humans and animals. Protein from microbial biomass is cheaper than animal proteins because the substrates used in the production are generally cheaper and more readily available. Moreover, the production process does not require arable land and the entire process can be completed within a short time. This article reviewed the process of SCP production. Different raw materials used in the production and variations in growth media preparation methods were discussed. Various sources of fermentation microorganisms and their potential substrate were reviewed. Growth media enrichment using different carbon, nitrogen, and mineral sources was also discussed.

Cooking takes the largest percentage of energy consumption and most households still depend on wood fuel energy, which contributes significantly to global warming. However, a major consideration for market infiltration of a green energy product is the willingness of consumers to pay for it. The study examined the determinants of willingness to pay for biofuel among small-scale food sellers in Ibadan metropolis. Primary data were collected from one hundred and fifty-five small-scale food sellers in Ibadan metropolis using a multi-stage sampling procedure. Data were analysed using descriptive statistics and probit regression. The majority of the food sellers were female (91.0%) with less than five household members (65.8%) and had tertiary education (40.0%). Most of them were not aware of biofuel (82.6%) but were willing to substitute firewood for biofuel (78.1%). The probability of willingness to pay for biofuel was increased by household size, being a female but reduced by bid at the unit price of ₦500 per litre. However, it was reduced by household size and education at ₦600 per litre. Based on the findings of this study, the entrepreneurs should take advantage of the emerging biofuel market by creating awareness of green energy and its benefits among the citizenry while government should support its production so that it can be sold at affordable price.