خيارات البحث
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The Effect of Common Mistletoe (Viscum album L.) Extracts, Collected from Different Host Plants, on Some Plant Pathogenic Bacteria
2020
Sabriye Belgüzar | Bahadır Şin | Zeliha Eroğlu | İzzet Kadıoğlu | Yusuf Yanar
In this study, antibacterial effects of semi-parasitic plant common mistletoe (Viscum album L.), collected from different woody host, extracts on the tomato bacterial cancer and wilt disease agent Clavibacter michiganensis subsp. michiganensis, pepper and tomato bacterial leaf spot disease agent Xanthomonas axonopodis pv. vesicatoria and tomato bacterial leaf spot disease agent Pseudomonas syringae pv.tomato were determined. The common mistletoe collected from pine, pear, acacia and mahaleb trees. The leaves and stems water extracts of common mistletoe were added to Nutrinet agar medium before autoclaving at the final concentrations of 1%, 2.5%, 5% and 10%. The bacterial concentration was adjusted to 108 cfu/ml with spectrophotometer to within an 0.2 at 600 nm. Then, 100 µl of bacterial inoculums were spread over the extracts amended media plates. As a control group, pathogens were plated on the unamended media. The study was established in 3 repetitions and repeated 2 times. At the end of the incubation period, bacteria growing on all treated petri dishes were collected and their density was measured in a spectrophotometer. Based on the results of the study, 1% and 2.5% concentration of the extracts obtained from leaves and stems of common mistletoe collected from different trees were not effective on the bacteria tested, while 5% and 10% concentration of them inhibited the bacterial growth completely (100%). Also, it was observed that there wasn’t difference on the pathogens on the basis of the host where mistletoe was collected. According to the results of this study conducted under in vitro conditions, in vivo studies should be carried out with the common mistletoe extract, which is effective on the bacterial pathogens.
اظهر المزيد [+] اقل [-]Antimicrobial Effect of Red Beet (Beta vulgaris var. Cruenta Alef.) On Some Foodborne Pathogens
2019
Oktay Tomar | Gamze Yıldırım
Red beet (Beta vulgaris var. Cruenta Alef.) it is a flowering plant belonging to the family of Amaranthaceae and is cultivated in the regions extending from the west of Europe to all the Mediterranean coasts In Turkey; especially in the Aegean and Marmara regions. In the World, it is used in the production of milk and milk products, fruit juices, sauces, soups, confectionery, jelly, tomato paste, breakfast products, sausages and sausage products. In Turkey, in addition to these, it is widely used, in producing the turnip juice and pickles. There beet is particularly rich in minerals such as sodium, potassium, magnesium, calcium and phosphorus and also contains iron and selenium. Further; rich in antioxidant phenolic substances, betalain and flavonoid. In this study; antibacterial effect of extracts obtained from red beet outer shell and flesh inner part by using different solvents (ethanol, methanol and distilled water) on some food pathogens were determined by dise diffusion method. As a result of the research; the most antimicrobial effect was observed in the water extract obtained from the shell portion of red beet against Listeria monocytogenes with a 17 mm dilution diameter. This value was determined to be followed by methanol extract obtained from the interior of the red beet against Pseudomonas aeroginosa with a 16 mm dilution diameter. The lowest MIC and MBC values were on the Enterobacter aerogenes of beet peel methanol extract with values of 23.44and 31.25 μg / mL, respectively. All results were compared with the reference values of Eucastand CLSI laboratories and resistance and sensitivity were determined.
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