The identification of plants in which the bees forage is key in establishing bee farms and increasing honey production. In this study pollen analysis of honey samples from the southeastern part of Nigeria was carried out to ascertain their floral sources and ecological origin. The honey samples were acetolyzed and microscopically studied to determine the pollen types. A total of seventy-one pollen types belonging to forty-one families of plants were identified. The honey samples were dominated by pollen grains from the families of Arecaceae, Euphorbiaceae, Myrtaceae, Irvigiaceae, Fabaceae, Combretaceae/Melastomataceae, and phyllanthaceae. Some of the dominant pollen grain identified include Elaeis guineensis, Alchornea cordifolia, Hymenocardia acida, Ocimum gratissimum, Syzygium guineense, Nauclea latifolia and Afzelia africana. Out of the six samples studied Njikoka sample was monofloral having Mimosa pigra as predominant pollen while Ayamelum, Ekwusigo, Nsukka, Ezeagu, and Udenu samples are multifloral containing Elaeis guineensis, Phyllanthus sp., Piliostigma reticulatum, Irvingia sp., Alchornea cordifolia, and Lannea sp. as major secondary pollen. All the samples analyzed have Elaeis guineensis in common except Ezeagu, indicating that these plants are present in all five locations. These results can also be used as a tool in the geographical identification of Southeastern Nigeria honey from other geopolitical zones.

Using functional foods which are recently a rising value in the food science arouses curiosity in terms of health among people. Honey, as a natural functional food, might play an important role in the increase of this value with a large but conscious use of it in kitchens. Having a prebiotic property with its oligosaccharide components, honey has a characteristic of supporting the increase of probiotic Bifidobacterium and lactobacilli bacteria. In the current study, the culinary status of honey was investigated depending on the views of professional chef trainers. For honey that shows different compositions, chefs point out that, because of the changes of nectars in the flora collected by bees, the qualitative honey bought from local producers could be a more functional food by using it with sauces, milk products like yoghurt and cheese, with such spices as ginger, turmeric, black pepper, and cinnamon at room temperature without an application of heat treatment.

The types and densities of honeyed plants vary depending on the altitude. This causes the amount and Physicochemical structure of honey produced in apiaries of different altitudes to change. In this study, honey harvest from the honeybee colonies placed at different altitudes in the same geographical region was carried out in the first week of September. Standard laboratory methods were used to determine some physicochemical properties of the honey samples. Some of the minimum and maximum average values obtained as a result of the analysis of honey samples; moisture 14.70% and 18.60%, free acidity 20.50 meq/kg and 25.30 meq/kg, pH 3.20 and 4.30, EC 0.22 and 0.44 mS/cm, fructose 32% to 45%, glucose 0.10% to 0.18%, sucrose from 0.66% 1.80%, maltose ranged from 0.66% to 1.80%. Also, HMF ranged from 1.80 mg/kg to 3.50 mg/kg, proline 530.00 mg/kg and 710.00 mg/kg, Density from 1.44 g/cm3 to 1.49 g/cm3, Invertase 20.30 U/kg-28.50 U/kg, Diastase activity 13.23-19.07 and Total phenolic content ranged from 76.00-94.00 g. It has been determined that the physicochemical structures of honey produced at different heights are statistically different from each other. This study aims to determine the effect of altitude difference on the quantity and physicochemical structure of honey.

Water kefir is a fermented probiotic beverage produced by fermentation of dried sugary fruits and sugar using water kefir grains. Sucrose is used as a sugar source in the traditional production of water kefir. The aim of this study is to determine the effects of grape molasses, honey and unrefined sugar on water kefir grains instead of sucrose. In the study, 4 groups were formed: grape molasses (10 g), honey (10 g), unrefined sugar (10 g) and refined sugar (10 g). 2 g of water kefir grain and 500 mL of drinking water were used for each group. And each group samples were fermented for 25°C 48 hours. The study continued for 5 weeks. The pH values and the titratable acidity values of water kefir samples and the weight of water kefir grains were determinated once every two day, while microbiological analyzes (Lactobacillus spp., Lactococcus spp. and yeast contents) and ethanol content of samples were determinate once every 15 days. In this study; it has been observed that the use of different sugars has no significant effect on the weights of the water kefir grains. In addition, it was determined that the Lactococcus spp. and yeast amounts in the water kefirs were higher in the samples produced by using honey and grape molasses. As a result, it was revealed that grape molasses, honey and unrefined sugar could be used as fermentation substrate in production of water kefir. At the same time, water kefir can be shown as an alternative fermented product for vegan consumers with allergies to dairy products.

Honey samples were obtained from wild and domesticated sources and analyzed for some physicochemical properties such as color, pH, moisture content, ash content, refractive index, specific gravity, total solid, viscosity, glucose and fructose content following Standard Association of Official Analytical Chemistry. The following range of values for pH (3.55-4.20), moisture content (18.50-25.60%), soluble solids (74.10-81.20%), ash content (0.08-0.14%), specific gravity (1.38-1.47), refractive index (81.3-83.4%), fructose content (40.5-63.04%) and glucose content (19.35-32.34%). The mineral composition analyzed revealed potassium to be the dominant mineral in the honey samples followed by Calcium. However, Cadmium and lead where not detected in the honey samples. The results indicated that parameters such as pH, moisture content, ash content, specific gravity, sugar (majorly fructose and glucose content), fructose/glucose ratio, glucose/water ratio conform within the limit of the international standard for honey. However, moisture contents of the wild honey samples (22.05% and 25.60%) were a little higher than the Codex Standards of ≤ 21%. In conclusion, the honey samples investigated have the needed quality criteria and are good for human consumption. The results also revealed excellent organoleptic acceptability of the honey samples, hence are suitable for human uses.

Honey samples were obtained from wild and domesticated sources and analyzed for some physicochemical properties such as color, pH, moisture content, ash content, refractive index, specific gravity, total solid, viscosity, glucose and fructose content following Standard Association of Official Analytical Chemistry. The following range of values for pH (3.55-4.20), moisture content (18.50-25.60%), soluble solids (74.10-81.20%), ash content (0.08-0.14%), specific gravity (1.38-1.47), refractive index (81.3-83.4%), fructose content (40.5-63.04%) and glucose content (19.35-32.34%). The mineral composition analyzed revealed potassium to be the dominant mineral in the honey samples followed by Calcium. However, Cadmium and lead where not detected in the honey samples. The results indicated that parameters such as pH, moisture content, ash content, specific gravity, sugar (majorly fructose and glucose content), fructose/glucose ratio, glucose/water ratio conform within the limit of the international standard for honey. However, moisture contents of the wild honey samples (22.05% and 25.60%) were a little higher than the Codex Standards of ≤ 21%. In conclusion, the honey samples investigated have the needed quality criteria and are good for human consumption. The results also revealed excellent organoleptic acceptability of the honey samples, hence are suitable for human uses.

The objective of this study was to develop a rapid infrared technique to determine 10 key quality parameters (sucrose, glucose, fructose, reducing sugar, 5-HMF, °Brix, moisture content, water activity, pH and free acidity) in honey by using new generation portable and handheld devices. The composition of honey samples (n=59) collected from different parts of Turkey was analyzed by using established reference methods, giving wide range of concentrations for each parameter. The levels of sucrose and 5-HMF in some samples were above the established regulatory limits (Codex Alimentarius and European Union standards), indicating possible adulteration or process and storage abuse. Spectra were collected by using portable Fourier-Transformed infrared (FTIR) and handheld NIR (Near Infrared) spectrometers. Partial least squares regression (PLSR) approach was used to correlate the spectral features with compositional reference values, giving strong linear correlation coefficients and standard errors of prediction. Although both systems performed similarly, portable FTIR system was superior in predictions of sucrose, 5-HMF and free acidity while portable NIR system performed noticeably better for °Brix and moisture content. The data indicates that all of the 10 parameters can be measured within the minutes using both systems, providing reliable screening capabilities, flexibility and the potential for in-field applications.

Honey is known to be a natural food product with antibacterial and wound-healing properties. Among its different types, some attributed more with medical applications including Manuka honey. In this study, some wound healing-related parameters were systematically investigated on two different types of Turkish honey (Chestnut and Anzer), in comparison with Manuka honey. Chestnut honey had considerable amount of hydrogen peroxide (5 μg/mL) whereas Manuka and Anzer honey did not contain any. Additionally, Chestnut honey had the highest acidity among all tested samples (24.50 meq/kg), as well as higher antioxidant activity (IC50 ; 2.45 mg/ml) than those of Anzer (13.02 mg/ml) and Manuka honey (6.27 mg/ml). It was also demonstrated that Chestnut honey displayed a considerable antimicrobial activity against selected pathogens. On the other hand, in the scratch assay performed as a model for the wound healing process, Anzer honey induced the highest cell proliferation corresponding to almost total wound closure for all concentrations tested. Overall, the results showed that the Turkish Chestnut and Anzer honey samples had distinguished characteristics for wound healing that is comparable to Manuka honey, and therefore are both promising candidates for use in clinical applications.

Honey is the most known and consumed bee product by consumers. Therefore, from the past to the present, the investigation of the factors affecting the supply and consumption of honey has been on the agenda of the researchers. For this purpose, in our survey study, the effects of honey sales carried out via television channels (commercials) on consumers were investigated. According to the survey results, it was determined that 87.91% of consumers had a negative view about honey sales performed via television, 3.54% had a positive opinion, and 8.55% had no opinion on the issue. It was found that 5.83% of consumers bought honey through television commercials, and the education, income, gender and number of individuals in their households have an effect on the tendency to buy honey. Regarding the exposing of companies selling fake or adulterated honey by the Ministry of Agriculture and Forestry, results showed that this situation positively affected 54.53 of consumers in terms of trust in honey positively, affected 13.30% of consumers negatively, and did not affect 13.30% of consumers in any way. As a result, deceptive honey commercials lead to consumer abuse and create an environment of distrust of honey. In order to minimize speculation on honey, it is seen necessary to increase deterrent penalties for businesses that lead to unfair competition.