This study aimed to identfy chemical composition, ruminal degradation characeristics and metabolizable energy (ME) content of five different chickpea line and a check cultivar’s straw using nylon bag technique. Feed samples were incubated as three replicates of each fistulated Holstein heifer for 0, 8, 12, 24, 36, 48, 72 and 96 h. Degradation characteristics of dry matter (DM) and neutral detergent fiber (NDF) in rumen were determined by using this mathematical expression D=a+b(1-e-ct). Crude protein (CP), acid detergent fiber (ADF), neutral detergent fiber (NDF), and ash contents of straw were ranged from 5.61 to 7.42%, 51.33 to 56.0%, 63.67 to 67.0%, and 8.0 to 9.0% respectively. Besides Rapidly soluble fraction (a), potantial degradability (a+b) and effective dry matter degradability (EDDM ) were ranged from 17.86 to 21.41, 54.40 to 59.43, 49.65 to 54.91% respectively. Estimated ME of chickpea entries straw were ranged from 5.96 to 7.37 MJ/kg. Metabolizable energy content of control chickpea cultivar was significantly higher than the other chickpea straw of lines. The research values of ME revealed that significant differences were determined among the lines in terms of energy content. In addition to, a strong relationship between straw NDF level and ME content were determined.