Root-knot nematodes (RKN), (Meloidogyne spp.), are the major biotic factor responsible for the limiting production of Kiwifruit in Nepal including Kiwifruit orchard of Warm Temperate Horticulture Center, Nepal. Hence, there is a pressing demand for nematicides that are both easily accessible and cost-effective while being environmentally friendly. A screenhouse experiment was conducted in the Summer of 2023 with an objective to evaluate the effects of different plant extracts, bio-control agents, and chemical nematicides against RKN on Kiwifruit seedlings. The experiment was set up in a Completely Randomized Design with three replications and eight treatments which include the extracts of Allium sativum and Lantana camara, Trichoderma viride, Pseudomonas fluorescens, Cartap hydrochloride, Fosthiazate, Inoculated control and Uninoculated control. The results revealed that Trichoderma viride proved to be the most effective in reducing the nematode population, displaying a low root gall index of 3.11, a minimal reproductive factor of 0.24, and a high percentage of nematode control at 91.71%. It was also found to be efficient in promoting the growth parameters of Kiwifruit seedlings. Additionally, regression analysis exhibited a significantly positive interaction between root gall index and reproductive factor, while indicating a negative interaction between reproductive factor and growth parameters. Therefore, T. viride (@ 20 gm per 2000 cm3 of soil) should be soil drenched before the seedlings are transplanted into the main field for effective and sustainable management of RKN. Nevertheless, further research is needed to determine the efficacy of T. viride in infested roots of Kiwifruit trees in field condition of Kiwifruit orchard.