Growing world population, scientists aim to achieve high-yielding products by using new techniques and methods in the fields of food, agriculture, and livestock. The aim of this study is to identify the DGAT/K232A gene polymorphism for Enhancing Performance Characteristics in Simmental cattle and to use it in breeding programs. DGAT/K232A gene polymorphism was analyzed by RFLP method in 70 Simmental cattle using CfrI restriction enzyme. The frequency of the K allele was found 0.77, while A allele was 0.23. The distribution of DGAT1-K232A genotype frequencies in the breed was not balanced (p<0.05). No significant relationship was found between DGAT1/K232Agene polymorphisms and milk yield due to the small number of samples.