Verticillium Wilt Disease is one of the most important diseases affecting the rate of cotton yield. There is no economic chemical control for Verticillium wilt, but it is recommended to use resistant varieties to control this disease. This experiment was carried out in a randomized plot design with four replications in the growth chamber to determine the response of some cotton cultivars against a defoliating and non-defoliating pathotypes of Verticilllium dahliae Kleb. In the study, a total of twenty cotton cultivars i.e. the resistant control GIZA 75, the tolerant control CARMEN and the susceptible control ACALA SJ2, defoliating (PYDV6 isolate) and non-defoliating (Vd 11 isolate) pathotypes were used, and cotton varieties were tested using conidial suspension technique. Analysis of variance showed significantly (P