In order to identify the effects of nutrients on gene expression and to assess the interactions between genes and nutrition by means of various cutting-edge technologies, the interdisciplinary branch ‘Nutrigenomics’ was created. Therefore, nutrigenomics corresponds to the use of knowledge and techniques of nutrition, genomics, transcriptomics, proteomics, epigenomics, and metabolomics to seek and explain the cross-talk between nutrition and genes in molecular level. Macronutrients are important dietary signals that control metabolic programming of cells and have important roles in maintaining cellular homeostasis by influencing specific gene expression. Recent advancements in molecular genetics studies, for instance, use of next-generation sequencing, microarray and qPCR array to investigate the expression of transcripts, genes, and miRNAs, has a crucial impact on understanding and quantitative measurement of the impact of dietary macronutrients on gene function. This review will shade a light on the interactions and mechanisms how the dietary source of macronutrients changes the expression of specific mRNA and miRNA. Furthermore, it will highlight the exciting recent findings in relation to animal performance characteristics which eventually help us to identify a dietary target to improve animal production.

Bu çalışmada, ticari faj preparatının çiğ sütten yapılmış beyaz peynirde Salmonella kontrolü üzerine etkisi araştırılmıştır. Bu amaçla Salmonella faj preparatı kullanılmıştır. Beyaz peynir üretimi 4 grupta yapılmıştır. Bunlar; 1-kontrol grubu (çiğ süt), 2-çiğ süt+patojen (Salmonella Typhi, %0,05), 3- çiğ süt+faj ilave edilen (%0,1), 4-patojen (%0,05) + faj (%0,1) grubu olarak belirlenmiştir. Beyaz peynir örneklerinde olgunlaşmanın 0., 30., 60. ve 90. günlerinde fizikokimyasal ve mikrobiyolojik analizleri yapılmıştır. Yapılan fiziko-kimyasal analizler; pH, titrasyon asitliği (ºSH), kuru madde, kuru maddede yağ, tuz ve kuru maddede tuz tayinidir. Mikrobiyolojik analizler; toplam aerobik mezofil bakteri, laktik asit bakterileri, Stafilokok spp., koliform grubu bakteri, Salmonella spp. ile faj ilave edilen peynir örneklerinde faj titresi analizidir. Ayrıca faj ilave edilen örneklerin sütlerinde ve peynir altı sularında faj titresi durumu incelenmiştir. Peynir örneklerinde, faj uygulamasının veya söz konusu bir patojen bulaşısının örnekler arasında fiziko-kimyasal ile genel mikrobiyolojik özellikleri bakımından farklılık gösterici etkisinin olmadığı elde edilen veriler sonucunda belirlenmiştir. Bu değerler tüm peynir gruplarında sadece süreye göre değişkenlik göstermiştir. Salmonella spp. sayısında ise 90. günde 2 ve 4 numaralı grup arasındaki fark kontrol grubuna göre kıyaslandığında istatistiksel olarak önemli bulunmuştur. Bunun haricinde Salmonella spp. sayıları gruplar arasında farklı bulunmamış, sadece olgunlaşmanın doğal süreci olarak günlere göre azalış göstermiştir. Bu çalışma sonucunda, çiğ sütten yapılan beyaz peynirlerde bakteriyofaj ilavesinin peynirin fiziko-kimyasal ve mikrobiyolojik özelliklerine etki etmediği ve faj etkisi ile patojen bakteri sayısında önemli bir fark olmadığı tespit edilmiştir.

The role of university partners in the innovation adoption process in the implementation of agricultural extension is very necessary to get attention to realize the success of empowering rice seed farmers. The purpose of this research is (1) to know the role of universities in the process of adopting innovation in IPB 3S seedling in Aceh Province, (2) to know the perception of farmers toward the characteristics of the innovation of IPB 3S varieties, and (3) to know the role of universities in the implementation process of extension agriculture to rice seed farmers. This research uses qualitative descriptive approach with data measurement using Likert scale. The results showed that in general the universities play significant role in innovation adoption process to rice seed farmers. The role is arranged by sequence; (1) implementing cultivation skill (2) strengthening farmer institution, (3) liaison with the government (4) guidance of transfer of technology, and (5) liaison with production market. Farmers perception on the characteristics of innovation optimum production technology package (IPB-Prima) IPB 3S (1) has a relative profitability, (2) easy to try, (3) conformity, (4) observable, and (5) innovation subtly level. The role of partners in the implementation of agricultural extension in sequence (1) conformity of extension materials, (2) intensity of extension, (3) appropriateness of extension method and (4) accuracy of media usage. The university should pay attention on the mechanism of facilitating technology transfer with the use of media and appropriate extension methods to rice seed farmers. The university also should build good communication with private organizations to help farmers in terms of seed price certainty produced by rice seed farmers in Aceh Province, Indonesia.

Developing alternative control methods such as using beneficial microorganisms and their metabolites to control the plant diseases has gained so much importance along recent years and research on this area are increasing day by day. In this study the possibilities of using microorganisms which have antimicrobial effects on controlling soil-borne fungi at strawberry production were investigated. Effects of different lactic acid bacteria (LAB) strains were studied in vitro and in vivo against the development of several soil-borne fungi. LAB were screened for antifungal activity by using cell free supernatant against Fusarium sp., Rhizoctonia sp., Macrophomina sp., Botrytis sp., Phtopythium sp., Cylindrocarpon sp. and Pestalotiopsis sp. Cell free supernatant of LAB isolates showed antifungal activity against fungi. In vitro effective strains of LAB were used in pot experiments to search their effects on fungal development and the development of the plant. While the antifungal effects of all LAB strains tested in vitro experiments exhibited promising results, in vivo experiments revealed similar effects on different fungi genera.

Bu çalışmada, Gümüşhane ilinde halk tarafından farklı tıbbi amaçlarla kullanılmakta olan atkuyruğu (Equisetum arvense) bitkisinin farklı polariteye sahip çözücülerle elde edilmiş ekstrelerinin toplam fenolik miktarı, antimikrobiyal ve antioksidan aktiviteleri incelenmiştir. Toplam fenolik miktarı gallik asit eşdeğeri olarak spektrofotometrik yöntemle ölçülmüştür. Antimikrobiyal aktivite, disk difüzyon ve mikrodilüsyon (MIC değeri) yöntemleriyle belirlenmiş; antioksidan aktivite ise ABTS [(2,2'-azino-bis (3-etilbenzotiazolin-6-sülfonik asit)] ve DPPH (2,2-difenil-1-pikrilhidrazil) yöntemleri kullanılarak tespit edilmiş ve troloks eşdeğeri olarak hesaplanmıştır. Toplam fenolik miktarı, en yüksek etil asetat ekstresinde (108,9 mgGAE/g ekstre) tespit edilirken, en yüksek antioksidan kapasite etanol ekstresinde 15,76 µg/ml troloks eşdeğeri olarak bulunmuştur. Ayrıca, en yüksek antimikrobiyal aktivite etanol ekstresinde MRSA’ya karşı 4 µg/ml MIC değeri olarak tespit edilmiştir.

The present study investigated the effects of dietary carob (Ceratonia siliqua) syrup supplementation on growth performance, haematological, serum biochemical and immunological parameters of tilapia, Oreochromis mossambicus. Five isonitrogenous and isoenergetic diets were formulated to contain carob syrup at levels of 0%, 5%, 2.5%, 1.25%, and 0.625%. Fish were fed experimental diets for 60 days. There were no particular differences in weight gain, feed conversion ratio, specific growth rate, red blood cell count, haematocrit ratio (%), serum total protein, albumin, and globulin levels of fish fed experimental diets. However, dietary carob syrup especially with 1.25% incorporation significantly decreased serum glucose, triglyceride, cholesterol levels. The dietary carob syrup especially at 1.25% significantly increased the phagocytic activity, phagocytic index, respiratory burst and potential killing activity. In conclusion, findings of the present study indicate that feeding tilapia with a diet containing 1.25% carob syrup over a period of 60 days might be adequate to improve immune parameters and serum biochemical variables without any adverse effect on growth performance and haematological parameters of fish.

Effects of storage duration, soaking time and parboiling temperature on cooking properties (cooking time, water uptake ratio, solid loss, cooked kernel length, and amylose) of Ofada rice was determined and optimised using response surface methodology. Storage duration, soaking time and parboiling temperature were 1, 5, and 9 months; 1, 3, and 5 days; and 80, 100, and 120°C. Data were analysed by ANOVA and regression analysis. The cooking time ranged between 14-38 min, water uptake ratio (WUR) 2.51-4.61, solid loss 1.47-4.78%, cooked kernel length 6.32-11.90 and amylose 17.34-26.28%. There exist significant differences in the cooking properties. The coefficient R2 ranged between 0.97-0.75 which is a positive indicator of the model fitness. Storage duration and parboiling temperature influenced cooking except in solid loss and cooked kernel length respectively. Effect of soaking time was found prominent in WUR and solid loss. Optimum treatment for quality cooking properties are storage of paddy for 5 months, soaking for 18h and parboiling at 80°C to yield 20 min cooking time, 4.22 water uptake ratio, 4.11% solid loss, 10.58 mm cooked kernel length and 25.08% amylose. The validated experiment yielded 21.41 min, 3.99, 2.73%, 8.20 mm and 26.39% for cooking time, water uptake ratio, solid loss, cooked kernel length and amylose respectively.

The primary purpose of this study was to determine factors influencing consumer preferences for UHT milk consumption in Erzurum province. The primary data used in this research was derived from Palandoken, Yakutiye and Aziziye districts of Erzurum province in 2010. The factor analysis was used to find out the factors affecting consumer preferences for UHT milk and to reduce these factors. As for the segmentation of consumers and bringing out the profile of each segment, cluster analysis was used. According to the results, 95.00% of households consumed UHT milk. 18 factors that are affecting the consumption of UHT milk were reduced to five main factors with factor analysis. The factor scores which determined with factor analysis were divided into three clusters by cluster analysis. UHT milk for consumers entering the first cluster has because of homogenous and packaging as well as intrinsic and extrinsic properties for advertising and price advantage is preferred. UHT milk for consumers entering the second cluster has ease of preparation and transportation, and confidential properties are preferred by reason. On the contrary, consumers entering the third cluster prefer to UHT milk for a good diet product.

Bu çalışmanın amacı, yüksek Cd konsantrasyonuna sahip toprağa çinko (Zn) uygulamaları ile ekmeklik ve makarnalık buğdayın Cd alımına olan etkisini araştırmaktır. Çalışma sera koşullarında Zn noksanlığına sahip kireç içeriği yüksek bir toprakta tesadüf blokları deneme desenine göre dört tekerrürlü olarak gerçekleştirilmiştir. Denemede Cd uygulamaları 0 (Cd 0) ve 10 (Cd10) mg kg-1 Cd dozlarında uygulanmıştır. Çinko uygulamaları ise topraktan 0 (Zn 0) ve 10 (Zn 10) mg kg-1 Zn ile toprak + yapraktan (Zn10+3Y; Zn10+8Y) olacak şekilde yapılmıştır. Yapraktan Zn uygulamalarına sapa kalkma döneminde başlanmış ve haftada 1 defa olmak üzere 3 ve 8 defa yapraktan %0,1 dozunda ZnSO4.7H2O uygulaması yapılmıştır. Bitkiler tane olgunluk döneminde hasat edilmiş ve tanelerde Zn ve Cd konsantrasyonları belirlenmiştir. Elde edilen sonuçlardan topraktan ve toprak+yapraktan Zn uygulamaları ile ekmeklik ve makarnalık buğdayların tane Zn konsantrasyonlarında önemli artışların olduğu görülmüştür. Toprağa Cd uygulaması ile Karacadağ çeşidine göre Harran-95 çeşidinin tanesinde daha fazla Cd birikimi olduğu görülmüştür. Karacadağ çeşidinin kontrol koşullarında 31 μg kg-1 olan Cd konsantrasyonu, Cd 10 koşullarında 3.616 μg kg-1’e, Harran çeşidinin ise 70 μg kg-1 tane Cd konsantrasyonu Cd10 koşullarında 8.434 μg kg-1’e yükseldiği görülmüştür. Bununla birlikte, toprak, toprak + yapraktan Zn uygulamaları, makarnalık buğday çeşitlerinin tane Cd konsantrasyonlarını önemli ölçüde azaltmıştır. Haran-95 çeşidinin Cd 10 koşullarında 8.434 μg kg-1 olan tane Cd konsantrasyonu, Zn10 koşullarında 6.464 μg kg-1’a, Zn10+3Y koşullarında 7.932 μg kg-1’a, Zn10+8Y koşullarında ise 6.745 μg kg-1’a düşmüştür. Sonuçlardan makarnalık buğday çeşidinin ekmeklik buğday çeşidine göre topraktan daha fazla Cd aldığı görülmüştür. Bunun yanında topraktan ve yapraktan Zn uygulamaları ile makarnalık buğday çeşidinin Cd alımında daha fazla azalma olduğu görülmüştür.

The traditional fermented food consumption has become very popular because of the increasing public concern about food additives. Lactic Acid Bacteria (LAB) species have traditionally been used as starter cultures in the production of fermented food. LAB can acquire antibiotic resistance from other bacteria in the natural environment and different resistant mechanisms via mutation. The resistance of bacteria to antibiotics is an increasingly important public health problem worldwide. In this study, antibiotic resistance of 115 Streptocoocus thermophilus and 35 Lactobacillus bulgaricus isolates obtained from traditional Turkish yogurts were tested against kanamycin, chloramphenicol, erythromycin, ampicillin, rifampicin, tetracycline, vancomycin and gentamicin using disc diffusion method. Study results showed that most strains were susceptible to all the antibiotics tested while a few of them were determined to be resistant only to kanamycin, ampicillin, erythromycin, and tetracycline. When contacted in a human body, resistant strains might transfer the related genes to the pathogenic species, which may result in devastating consequences.