In this study, it was investigated that the inhibition effect of some lactic acid bacteria (Lactobacillus acidophilus NCC68, Lactobacillus casei Shirota, Lactobacillus rhamnosus (Ezal, commercial starter cultures)) which possessed with probiotic characteristics, against Bacillus cereus, Salmonella Enteritidis, Escherichia coli, Escherichia coli 0157:H7 ATCC 35150 and Staphylococcus aureus ATCC 25923. Besides, the inhibitory effect of probiotic cultures which used with meat and meat product additives that garlic extract over the antagonistic effects of sensitive pathogens were investigated in vitro. Consequently, the whole of lactic acid bacteria and garlic extract which were used in this study, showed inhibition effects against all selected pathogenic bacteria. Staphylococcus aureus ATCC 25923 was determined as the most sensitive pathogenic bacteria while Bacillus cereus was the most resistant bacteria against lactic acid bacteria and garlic extract. There was a distinctive increase in inhibition effects were observed by used of a combination with lactic acid bacteria and garlic extract.

Coffee cultivation depends on water supplied by rain or irrigation, which, in turn, affect productivity and harvest distribution. Knowing the influence of plant spacing on the behavior of soil moisture and water consumption, is likely to determine a crop’s planting density, fertilization and planting times, all based on regional water availability. In this context, the study was conducted at Naranjal Experimental Station, municipality of Chinchiná (Caldas, Colombia) at 04° 58’ N, 75° 39’ W; 1,381 m a.s.l., aimed to describe moisture performance in a coffee plantation established on  an Andisol, under three planting densities. This was done by estimating the evapotranspiration (ETo) and assessing crop evapotranspiration (ETc). Soil moisture was measured with a capacitance probe equipped with sensors spaced at 10, 20, 30, 40, 60 and 100 cm. Moisture content in the soil profile varied with planting density and from dry to humid periods. ETo and ETc were observed to be influenced by weather conditions, which determined higher values during the dry season and lower ones in the rainy season. Although planting density was found to affect hydraulic soil properties, sufficient water supply for the plants along both humid and dry seasons was stabilized by the water storage capacity that characterizes Andisols. The capacitance probe constitutes an effective tool for studying water performance in soils with coffee vocation in Colombia, especially when they are sensitive to the limitations imposed by water deficit resulting, in turn, from current or future climate variability. | El café es un cultivo dependiente del agua disponible en el suelo, bien sea a través de la lluvia o del riego, ya que ésta tiene un marcado efecto en la productividad y la distribución de la cosecha. Conocer el efecto de la distancia entre plantas sobre el comportamiento de la humedad en el suelo y el consumo de agua, influye en las prácticas agronómicas del sistema productivo del café, entre ellas: la densidad de siembra, la fertilización, y las épocas de siembra. El estudio se realizó en la Estación Experimental Naranjal ubicada en Chinchiná (Caldas, Colombia) a 04° 58’ N, 75° 39’ O; 1,381 m de altitud y tuvo como objetivos conocer el comportamiento de la humedad en un Andisol sembrado con café en tres densidades de siembra, estimar la evapotranspiración de referencia (ETo) y evaluar la evapotranspiración del cultivo (ETc). La humedad del suelo se midió con una sonda de capacitancia de 100 cm con sensores espaciados cada 10 cm. La humedad en el perfil del suelo varió con la densidad de siembra y entre los periodos secos y húmedos considerados. La ETo y la ETc fueron influenciadas por las condiciones de clima, siendo mayores en la época seca y menores en la época de lluvias. La densidad de siembra afectó las propiedades hidráulicas del suelo. No obstante, por la capacidad de almacenar agua que caracteriza a los Andisoles, las plantas tuvieron agua disponible aún en las épocas secas. La sonda de capacitancia mostró ser un instrumento efectivo para el estudio del comportamiento del agua en los suelos de la región cafetera de Colombia, especialmente aquellos sensibles a las limitaciones ocasionadas por el déficit hídrico como producto de la variabilidad climática actual y futura.

Abstract Coffee cultivation depends on water supplied by rain or irrigation, which, in turn, affect productivity and harvest distribution. Knowing the influence of plant spacing on the behavior of soil moisture and water consumption, is likely to determine a crop’s planting density, fertilization and planting times, all based on regional water availability. In this context, the study was conducted at Naranjal Experimental Station, municipality of Chinchiná (Caldas, Colombia) at 04° 58’ N, 75° 39’ W; 1,381 m a.s.l., aimed to describe moisture performance in a coffee plantation established on an Andisol, under three planting densities. This was done by estimating the evapotranspiration (ETo) and assessing crop evapotranspiration (ETc). Soil moisture was measured with a capacitance probe equipped with sensors spaced at 10, 20, 30, 40, 60 and 100 cm. Moisture content in the soil profile varied with planting density and from dry to humid periods. ETo and ETc were observed to be influenced by weather conditions, which determined higher values during the dry season and lower ones in the rainy season. Although planting density was found to affect hydraulic soil properties, sufficient water supply for the plants along both humid and dry seasons was stabilized by the water storage capacity that characterizes Andisols. The capacitance probe constitutes an effective tool for studying water performance in soils with coffee vocation in Colombia, especially when they are sensitive to the limitations imposed by water deficit resulting, in turn, from current or future climate variability. | Resumen El café es un cultivo dependiente del agua disponible en el suelo, bien sea a través de la lluvia o del riego, ya que ésta tiene un marcado efecto en la productividad y la distribución de la cosecha. Conocer el efecto de la distancia entre plantas sobre el comportamiento de la humedad en el suelo y el consumo de agua, influye en las prácticas agronómicas del sistema productivo del café, entre ellas: la densidad de siembra, la fertilización, y las épocas de siembra. El estudio se realizó en la Estación Experimental Naranjal ubicada en Chinchiná (Caldas, Colombia) a 04° 58’ N, 75° 39’ O; 1,381 m de altitud y tuvo como objetivos conocer el comportamiento de la humedad en un Andisol sembrado con café en tres densidades de siembra, estimar la evapotranspiración de referencia (ETo) y evaluar la evapotranspiración del cultivo (ETc). La humedad del suelo se midió con una sonda de capacitancia de 100 cm con sensores espaciados cada 10 cm. La humedad en el perfil del suelo varió con la densidad de siembra y entre los periodos secos y húmedos considerados. La ETo y la ETc fueron influenciadas por las condiciones de clima, siendo mayores en la época seca y menores en la época de lluvias. La densidad de siembra afectó las propiedades hidráulicas del suelo. No obstante, por la capacidad de almacenar agua que caracteriza a los Andisoles, las plantas tuvieron agua disponible aún en las épocas secas. La sonda de capacitancia mostró ser un instrumento efectivo para el estudio del comportamiento del agua en los suelos de la región cafetera de Colombia, especialmente aquellos sensibles a las limitaciones ocasionadas por el déficit hídrico como producto de la variabilidad climática actual y futura.

2012 yılında yürürlüğe giren 6360 sayılı büyükşehir yasası ile büyükşehirlerde kır kent ayrımı gözetilmeyerek büyükşehir belediyelerinin hizmet götürme alanı il mülki sınırını kapsayacak şekilde genişletilmiştir. Böylece büyükşehir konumunda yer alan illerde tüzel kişiliğe sahip olan köy ve belde yerleşim yerlerinin tüzel kişilikleri kaldırılarak mahalleye dönüştürülmüştür. Durum böyle olunca bugüne kadar kentsel alana yönelik hizmet anlayışına sahip belediyelerin daha önce köy olan mahallelere belediye hizmetlerini götürmek, tarımsal alt yapı hizmetleri, hayvancılık yatırımları ve faaliyetlerini desteklemesi gibi yeni görev alanları ortaya çıkmıştır. Bu çalışma ile 6360 sayılı büyükşehir yasasının kırsal yaşama olası etkileri araştırılmıştır. Bu kapsamda Kocaeli ili Gölcük İlçesine bağlı Nüzhetiye köyü seçilmiştir. Yeni yasa ile artık Nüzhetiye mahallesi olan mahalle sakinlerine, yeni büyükşehir yasasından haberdar olup olmadıkları ve olumlu ya da olumsuz yönde etkilenip etkilenmedikleri sorulmuştur. Elde edilen bulgular ışığında önerilerde bulunulmuştur.

Plant genetic improvement has a double scientific nature: it is both an empirical science of nature and an applied science of design. In this context, this paper provides an investigation of scientific prediction as an essential fea-ture of plant genetic improvement as an applied science of design with special emphasis in the improvement of stone fruits (Prunus genus). In considering plant genetic improvement, we can speak of three levels of knowledge. These levels are associated with molecular biology (micro level); the genetic constitution of an individual (meso level); and the phenotype and overall appearance of the new release (macro level). These levels of reality affect both the type of prediction and the methodology applied. The prediction is the main objective of the plant genetic improvement as science of design. It is very important to have a knowledge of possible future to make a new design that will be complete within a few years, more than 12 years in the case of stone fruit that is the subject of this work. Finally, in developing a scientific prediction applied to Prunus breeding it is necessary to consider different internal (the genetic nature of the starting plant material, the available methodologies, etc.) and external (social acceptation, environmental factors, biotic and abiotic stresses, etc.) variables.The degree of knowledge of these variables determines the quality of the prediction in the design of the new Prunus varieties. | La mejora genética vegetal tiene una doble índole científica: Ciencia Empírica de la Naturaleza y Ciencia Aplicada de Diseño. En este contexto el presente trabajo pretende indagar en la predicción científica como rasgo esencial de esta disciplina en cuanto Ciencia Aplicada de Diseño con especial referencia la mejora de frutales de hueso (del género Prunus). En mejora genética vegetal podemos hablar de tres niveles de conocimiento asociados a la biología molecular (nivel micro), la constitución genética de un individuo (nivel meso) y al fenotipo o aspecto global de la nueva variedad (nivel macro), que afectan tanto a los tipos de predicción como a las metodologías a aplicar. La predicción constituye el objetivo principal de la mejora genética como Ciencia de Diseño. Es clave tener un conocimiento del futuro posible para poder hacer un nuevo diseño que estará completo al cabo de algunos años, más de 12 años en el caso de los frutales de hueso que es el objeto de este trabajo. Asimismo, es necesario tener en cuenta en el desarrollo de esta predicción científica aplicada a la mejora de Prunus diversas variables internas (la naturaleza genética del material vegetal de partida, las metodologías disponibles, etc.) y externas (la aceptación social, los factores medioambientales, los estreses bióticos y abióticos, etc.) a la hora de llevar a cabo esta predicción. El grado de conocimiento de estas variables determinará la calidad de la predicción en el diseño de nuevas variedades de Prunus.

Applying different technological processes during the production of food has a lethal effect on the bacteria but DNA of these bacterial strains may cause false positive results when detected by real time PCR technique because they preserve their existence for a certain period of time. To overcome this shortcoming of the real time PCR technique, a new method has been developed in recent years, based on the removal of dead cell DNA from the medium by treatment with Propodium Monoazide (PMA) before DNA extraction. In this study, real-time PCR method was combined with PMA application for the detection of live cells of Salmonella Typhimurium in heat treated milk samples. For this purpose, milk samples inoculated with S. Tyhimurium were heat treated at different temperatures (60, 65, 70 and 75°C) and times (15, 60, 300, 900 sec) and number of live bacteria was determined comparatively by direct real-time PCR, PMA/real-time PCR and conventional cultural method. As a result, unlike the direct real time PCR technique, PMA/real-time PCR method prevents to a certain extent of false positive results from dead cells at all tested temperatures and times but higher results were obtained from PMA/real-time PCR method when compared to conventional cultural results. Therefore, further studies should be carried out to optimize the conditions of the PMA application in order to eliminate the high positive results detected by the PMA / real-time PCR method

186 accessions of 9 species of Brachiaria were established in a design of fringes divided on 2 repetitions. In the third year seeds were evaluated to test their potencial under 2 different fertility levels: a) One recommended in order to establish pastures; b) Other in order to establish a system composed rice-pasture. The yield and the seed weight unit did not present significant differences (P | 186 accesiones de 9 especies de Brachiaria, se establecieron en un diseño de franjas divididas, con 2 repeticiones, para evaluar 2 niveles de fertilidad: a) el recomendado para establecer pasturas; b) el nivel para establecer un sistema integrado arroz-pastos. En el tercer año se evaluó el potencial de rendimiento de semillas. En las pruebas de germinación y latencia se evaluó el efecto de 2 tratamientos: 1) Agua y 2) Semilla escarificada en ácido sulfúrico; las de b. humidicola se trataron con Nitrato de Potasio. El rendimiento promedio y el Peso Unidad de las semillas no presentaron diferencias significativas (P

Meat is one of the main nutrition source in the human diet with its excellent protein, vitamin and mineral contents. Despite its advantages, being high-priced makes meat products open to adulteration. Meat products are mixed food types which can contain different species of meat. However, mixing two or more types of meats is not always allowed by laws. On the other hand, replacement high quality meats with cheaper meat types are a cost lowering way for the producers. The commonly consumed meat types differ from country to country, but generally economical, ethnic and religion concerns are in the foreground. In this case, species identification techniques are gaining importance. Although some techniques depending on DNA or spectroscopy have been developed for many years, choosing the best method for species identification is still among the controversial issues today. Thus, the currently used methods and promising techniques in this area were discussed in this review.

In strawberry, potassium fertilization has been little studied, which difficult management strategies for an increasing yield and understanding the use of this nutrient in strawberry crop. The aim of this study was to evaluate the influence of sources and rates of potassium (K) fertilizers on yield, K level and agronomic traits of strawberry cv. Camarosa fruit, carried out under greenhouse conditions. A randomized in a completely block design with four replicates in a factorial arranged 6x3 (six rates of potassium x three sources of potassium fertilizers), was performed. Fertilizers sources used were as follows: potassium sulphate, potassium nitrate and potassium chloride in 0, 60, 120, 180, 240 and 300 kg ha-1 K2O doses. An increasing potassium rates have allowed a higher potassium content in leaves and fruits. A higher potassium content in leaves was obtained by KNO3 (11.70 g kg-1). Yield, fruit number per plant and potassium extraction per plant, increased linearly with the evaluated doses and fruit average weight at a dose of 183 kg ha-1. There was no significant difference among potassium content in leaves. Strawberry production is maximized through potassium fertilization, independent of the source origin. | En fresa, se ha estudiado poco la fertilización potásica, lo que complica las estrategias de manejo para aumentar la productividad, y para la comprensión de la utilización de este nutriente en el cultivo de la fresa. El objetivo de este estudio fue evaluar la influencia de las fuentes y dosis de fertilizantes de potasio (K) en el rendimiento, la concentración de K, y las características agronómicas de la fruta de fresa cv. Camarosa, cultivada en condiciones de invernadero. El diseño experimental fue completamente al azar, con cuatro repeticiones, en un 6x3 factorial (seis tasas de K x tres fuentes de fertilizantes potásicos). Las fuentes utilizadas fueron sulfato de potasio, nitrato de potasio y cloruro de potasio aplicado en las siguientes tasas: 0, 60, 120, 180, 240 y 300 kg ha-1 de K2O. Las tasas crecientes de potasio permitieron un mayor contenido de potasio en hojas y frutos. El contenido de potasio más alto a nivel foliar más alto fue obtenido a través de KNO3 (11,70 g kg-1). El rendimiento, número de frutos por planta y la extracción de potasio por planta, aumentó linealmente con las dosis evaluadas y el peso medio del fruto a una dosis de 183 kg. ha-1. No hubo diferencia significativa entre las fuentes de potasio para el contenido foliar de potasio. La producción de fresa se maximiza mediante la fertilización potásica, independientemente del origen de la fuente.