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Bazı Anadolu Yerli Sığır Irklarının MtDNA D-loop Dizi Analizi: Genetik Çeşitlilik ve Popülasyon Geçmişinin Değerlendirilmesi النص الكامل
2023
Müge Doğan | Mehmet Nizamlıoğlu
Maternal kalıtımın moleküler göstergesi olan mitokondrial DNA, genomik DNA‘ya oranla daha hızlı evrimleşmesi, rekombinasyonun olmayışı ve türlerin coğrafi dağılımına göre farklılıklar göstermesi gibi özelliklerinden dolayı popülasyon genomiği, filogenetik ve filocoğrafik çalışmalarda sıklıkla tercih edilen belirteçlerden birisidir. Bu çalışmada Anadolu'da yetiştirilen bazı yerli sığır ırklarının, ırk içi ve ırklar arası genetik çeşitlilikleri ile filogenetik ilişkilerinin ortaya konulması amacıyla mitokondrial DNA D-Loop bölgesi dizi analizleri gerçekleştirildi. Öncelikle araştırmaya konu olan sığır ırklarının örnekleme çalışması tamamlandı. Standart Fenol/Kloroform Yöntemi kullanılarak DNA izolasyonu yapılan örneklerin mitokondrial DNA D-Loop bölgesinin yükseltgenmesi ise Polimeraz Zincir Reaksiyonu ile yapıldı. DNA dizi analizi sonrası yerli sığır ırklarına ait veriler GenBANK’ tan alınan referans mitokondrial DNA dizileri ile birlikte hizalandı. Haplotip ve nükleotid çeşitlilikleri ile popülasyon içi ve popülasyonlar arası genetik ve filogenetik ilişkileri değerlendirildi. Elde edilen veriler ile Anadolu yerli sığır ırklarının, nükleotid ve haplotip çeşitliliği, haplotip sayıları ile popülasyon içi ve popülasyonlar arası varyasyonun oldukça yüksek olduğu belirlendi. Anadolu da yetiştirilen yerli sığır ırklarının filogenetik olarak Asya ve Avrupa sığır ırkları arasında yer aldığı sonucuna varıldı.
اظهر المزيد [+] اقل [-]Tekirdağ İli Tarım Parsellerinin Arazi Toplulaştırma Açısından Şekil Analizi ile Değerlendirilmesi النص الكامل
2023
Erdem Kurşun | Fatih Konukcu | Bahadır Altürk
Şekil bozukluğu, tarımsal etkinlikleri ve kırsal kalkınmayı olumsuz yönde etkileyen en önemli etmenlerden birisidir. Bu araştırmada, Tekirdağ iline ait 346.740 tarım parselinin şekil uygunluğu altı farklı kriterden (kenar uzunluğu, dar açı sayısı, geniş açı sayısı, köşe sayısı, kompaklık ve düzenlilik) oluşan Parsel Şekil İndeksi (PŞİ) yöntemiyle belirlenmiştir. PŞİ yöntemi, bir geometriyi diğer klasik şekil yöntemlerinden çok daha kapsamlı bir şekilde analiz etmektedir. Parsellerin şekil uygunluğunun bu yöntemle tespit edilmesi ve sonrasında şekil bozukluğu olan parsellerde yapılacak düzeltmeler özellikle tarımsal mekanizasyonun olumsuz etkilerinin en aza indirilmesini sağlamaktadır. Çalışmada, şekil uygunluk dereceleri “çok bozuk şekilli parseller”, “bozuk şekilli parseller”, “uygun şekilli parseller” ve “ideal şekilli parseller” olmak üzere dört farklı şekilde sınıflandırılmıştır. Elde edilen sonuçlara göre; ildeki parsellerin %17,27’sinin çok bozuk şekilli, %35,21’inin bozuk şekilli, %35,54’ünün uygun şekilli ve %11,98’inin ise ideal şekilli geometriye sahip olduğu belirlenmiştir. Üretimi olumsuz etkileyen şekil bozukluğunun il genelinde fazla olmasından dolayı arazi toplulaştırma çalışmalarının ivedilikle yapılması gerekmektedir. PŞİ yöntemi, Türkiye’de planlanan arazi toplulaştırma projeleri öncesi mekânsal yer seçiminde, bölge, havza, köy veya mahalle ölçeğinde büyük fayda sağlayabilir.
اظهر المزيد [+] اقل [-]Farklı Kurutma Ajanları Eklenmesinin Dondurarak Kurutulmuş Hünnabın Fizikokimyasal Özellikleri ve Sorpsiyon Davranışı Üzerine Etkisi النص الكامل
2023
Banu Koç | Tarık Yörükoğlu
Bu çalışmada, inülin, gam arabik ve peynir altı suyu tozu gibi kurutucu ajanların eklenmesi ile dondurularak kurutulmuş hünnap tozunun, fiziksel özellikleri (yığın, partikül ve sıkıştırılmış yoğunluk, akabilirlik, gözeneklilik, partikül boyutu, higroskopiklik, kekleşme derecesi ve rekonstitüsyon özellikleri), kimyasal özellikleri (nem içeriği, renk, camsı geçiş sıcaklığı, toplam fenolik madde ve antioksidan aktivite) ve nem sorpsiyon izotermleri incelenmiştir. Kurutucu ajan olarak peynir altı suyu eklenmiş dondurarak kurutulmuş hünnap tozunun nem içeriği, higroskopi, Carr indeks, Hausner oranı ve kekleşme derecesi değerleri kurutucu ajan eklenmemiş (kontrol) hünnap tozundan daha düşük olmasına rağmen, camsı geçiş sıcaklığı, yığın ve sıkıştırılmış yoğunluk değerleri daha yüksek bulgulanmıştır. İnülin (74,07) ve peynir altı suyu tozu (72,07) eklenmiş hünnap tozunun parlaklık değerleri, gam arabik (66,56) eklenmiş hünnap tozu ve kontrole (65,85) göre daha parlak olduğu belirlenmiştir. Hünnap tozlarının hepsinde, nem sorpsiyon izotermleri sıcaklığa ve kurutucu ajanının tipine bağlı olarak değişmekte ve denge nem içerikleri ise artan sıcaklıkla birlikte azalmaktadır. Peynir altı suyu tozu içeren hünnap tozunun tek tabaka denge nem içeriği diğer tozlardan daha düşük çıkmış (GAB modeline göre 25°C için M0=0,072 g·g-1 kuru katı ve 40°C için M0=0,062 g·g-1 kuru katı) ve bu nem içeriğini sırasıyla inülin, gam arabik kurutucu ajan eklenmiş hünnap tozları ve son olarak kontrol grubu izlemiştir.
اظهر المزيد [+] اقل [-]Assessing Vegetable Farmers’ Knowledge of Disease and Pests Control Methods in Ghana: A Survey of Tomato (Solanum lycopersicum [L]) Farmers in the Mampong Municipality of the Ashanti Region of Ghana النص الكامل
2023
John Bokaligidi Lambon | Mahama Sanuel | Joseph Honger | David Yakubu
A survey of 200 farmers in the Mampong Municipality in the Ashanti region was conducted between June and October 2016. It was to determine their knowledge of different pests and disease control methods employed, access to extension services, pesticides use and other pests control methods. The study relied on data collected from respondents, 12 key informants (pesticides dealers, vegetable buyers and local chief farmers), field surveillance and observation as well as the reports of the Directorate of Agriculture in the Municipality. Data were captured and analyzed using MS Excel and Statistical Package for Social Sciences (SPPS) version 16 (SPSS Inc., Chicago, IL) and interpreted into simple percentages on tables and charts. The result showed that farmers are above 20 years and 18% were female. The majority (74%) have at least basic education with six (6) or more years’ of experience in tomato production. Sixty-four percent (64%) had no access to extension services due to poor contact with the agricultural extension agents (AEAs). Most respondents (70%) lacked education on pesticides and alternative (e.g., integrated pests’ management) control. Nine-two percent acknowledged the dangers of pesticides to public health. Sixty-four percent practice bi-weekly calendar spraying while 30 % and 6% practice weekly and occasional spraying respectively. Forty-four percent of farmers throw used containers or leave them on the farm. More education is needed to ensure safe use of pesticides and wholesome tomatoes for the public.
اظهر المزيد [+] اقل [-]Determinants of Choice of Marketing Outlet for Edible Insects among Smallholder Farmers and Traders in Western Kenya النص الكامل
2023
Lydia Akinyi Omondi | Mary Orinda
The edible insect sector has, in recent times, gained significant prominence and attention. Particularly, the government of Kenya has made remarkable steps to achieve a potentially large and valuable edible insect market, with a significant milestone being the passing of regulations on edible insects as a new source of proteins. However, research on the edible insect marketing environment is still indistinct. Therefore, the study sought to evaluate the determinants of the market outlets preferred or used by smallholder farmers and traders of domesticated and field-collected edible insects (including cricket, bees, winged termites, lake flies, and dung beetle) in Siaya and Vihiga counties. This cross-sectional study was done among 188 edible insects’ farmers and traders. Purposive sampling identified the study area, while snowball sampling reached the study participants. Data was collected using structured questionnaires and analyzed using multinomial logit regression to assess independent-dependent variable relationships, yielding marginal effects. Study findings showed that at 95% confidence interval, the yield was significant to the three outlets used by the farmers. Gender was significant to both institution and open-air markets outlets but insignificant to selling at the farm gate. Age, education level, and experience in marketing were insignificant to all the three market outlets. Marketing training was significant to the institution and open-air markets. The study also showed that farmers and traders had limited choices to sell their edible insect produce, which was majorly affected by yield and age variables. In view of these findings, enhancing edible insect marketing and training through initiatives that would increase production among farmers and breaking the attitudes toward open-air marketing among male farmers is pivotal to the thriving of the novel food enterprise towards achieving food security in the region.
اظهر المزيد [+] اقل [-]Phenotypic and Pathogenic Characterization of Leaf Fungi of Yam (Dioscorea spp) Varieties Grown In Côte D’Ivoire النص الكامل
2023
Ganemtore Souleymane | Camara Brahima | Kamara Adjata | Kassi Koffi Fernand Jean-Martial | Kone Daouda
Yam (Dioscorea spp) occupies an important place in the diet of the populations of Côte d’Ivoire. It is a real source of starch and therefore generates enormous commercial potential. However, the decrease in production due to pest attacks represents a real threat to this crop. This study was conducted with the aim of improving yam production in Côte d’Ivoire. To do so, isolations carried out on yam leaves showing symptoms of foliar diseases have allowed us to identify 9 fungal genera. These were Colletotrichum sp., Fusarium sp., Pestalotiopsis sp., Pestalotia sp., Botryodiplodia sp., Aspergillus sp., Mucor sp., Curvularia sp. and Phytophtora sp. Among these fungi, the genus Colletotrichum sp. was the most isolated with a rate of 56% followed by the Fusarium and Pestalotia genera (8%). Pathogenicity tests performed on healthy leaves of two yam varieties revealed that the Dioscorea alata is more susceptible to fungi compared to Dioscorea rotundata. The largest average diameter of necrosis was caused by Pestalotiopsis sp. (5.97 cm) on the Dioscorea alata variety while the smallest was caused by Colletotrichum sp.9 on Dioscorea rotundata (0.5 cm). Combatting these fungi need to be developed for effective management of leaf diseases of yam in Côte d’Ivoire.
اظهر المزيد [+] اقل [-]Selçuk University Museum Interior Design and Application Example النص الكامل
2023
Ali Akçaova | Mehmet Noraslı
The aim of this study is to cover the design-implementation processes of the museum interiors of the museum building in Selçuk University campus, which consists of foyer, temporary and permanent exhibition areas that can realize the aims and functions of the museum in accordance with the culture of the institution to which it is affiliated. Two types of materials were used within the scope of the study. The first type of material is theoretical and visual information based publications, thesis studies, lecture notes, architectural and interior design printed publications and internet resources. The information scanned and analyzed was handled from general to specific. The second material is the analysis of the Selçuk University Museum building located in Selçuk University Alaeddin Keykubat Campus by the authors with the on-site identification stages, interviews with the relevant people, the requirements of the museum foyer, temporary, permanent exhibition areas and existing interior photographs. The method followed in the study is observation and detection. As a result of the researches and observations made by utilizing publications, thesis studies, course notes, architectural and interior architectural printed publications and internet resources on the subject, an identification study was carried out in the existing museum building. As a result of the research, it was stated that the museums within the university campus have an important place in terms of establishing a bond between the public and the university, apart from the educational areas affiliated to the institution. Apart from education and training structures, from a different perspective, it was mentioned that they are common multi-purpose areas and the positive aspects they add to the institution to which they are affiliated. The interior revisions made in the determined areas of the museum building, the project design process and the implementation phases were mentioned.
اظهر المزيد [+] اقل [-]Comparison of Different Extraction Methods on the Recovery Efficiencies of Valuable Components from Orange Peels النص الكامل
2023
Mehmet Emin Argun | Aslıhan Öztürk | Mustafa Şamil Argun
Supercritical-CO2 extraction, Soxhlet extraction, and ultrasound-assisted extraction methods were conducted in this study to recover valuable components, specifically phenolic antioxidant compounds, from orange peels. Basic operating parameters such as temperature and pressure, which affect the extraction efficiency of phenolic substances in orange peel with supercritical-CO2, were designed using the central composite design methodology. In the Soxhlet and ultrasound-assisted extraction methods, 2-hour extraction processes were carried out using ethanol at different concentrations (50%, 80% and 100%) as a solvent. Yield comparison was made by performing total phenolic content, antioxidant activity and total flavonoid content analyses in the extracts. The total phenolic content (TPC) in the extracts was determined to be 5034 mg GAE/L for supercritical-CO2 extraction at 61.5°C and 20 MPa. In comparison, Soxhlet extraction yielded a TPC of 1728 mg GAE/L, while the ultrasound-assisted extraction method resulted in a TPC of 4056 mg GAE/L. It was determined that the optimum operating parameters of supercritical-CO2 extraction were 60°C and 26.4 MPa in case all the responses were maximized. The best phenolic recovery was obtained at 100% ethanol in Soxhlet extraction and 80% ethanol in ultrasound-assisted extraction. Although supercritical-CO2 extraction is an environmentally friendly application, the recovery rate of valuable components from raw materials is lower than in Soxhlet extraction and ultrasound-assisted extraction. However, since the volume of the extracts obtained from the supercritical-CO2 extraction is small, the ratio of phenolic compounds is higher.
اظهر المزيد [+] اقل [-]Mapping the Dispersion Pollution Load of Animal Waste and Investigating its Environmental Effects: The Case of Karaman النص الكامل
2023
Yusuf Alparslan Argun | Özgür Çakmakcı
Animal wastes are not stored correctly and are used haphazardly without control in agricultural lands. As a result, it causes many irreparable environmental pollution, especially water pollution. These wastes, which are described as diffuse pollution, cause pollution of both underground and surface water resources directly or indirectly and even make them unusable. In this research, waste generation as a result of animal breeding in Karaman province, its districts, neighborhoods and villages and the effects of animal wastes on environmental pollution were evaluated with distributed pollutant load calculations. In the study, the number of 1019277 ovine and 81368 bovine in Karaman in 2022 was used. The total nitrogen (TN) produced annually by the animals has been calculated as 1,723.23 tons/year, and the total phosphorus (TP) amount is determined as 124.23 tons/year. Additionally, for large ruminant animals, the annual total amount of dry manure is 130,305.77 tons, and for small ruminant animals, it is 41,984.27 tons. To prevent environmental pollution, these wastes should be stored in closed areas in compliance with standards, and processes such as composting, drying, and biogas production should be applied. By doing so, not only can environmental pollution be mitigated but also economic value can be obtained. The proper management and utilization of these wastes have high economic potential and can contribute to sustainable development, supporting the country’s economy. In addition, this study is a source for researchers working in the field in calculating the pollution load of animal wastes and is thought to be a guide for decision makers and practitioners.
اظهر المزيد [+] اقل [-]Screening of Promising Maize Varieties Against Maize Weevil (Sitophilus zeamais Motschulky) Under Storage Condition النص الكامل
2023
Sarjit Pandey | Dharma Raj Katuwal | Rashmi Aryal
The maize weevil (Sitophilus zeamais Motschulsky) causes significant quantitative and qualitative losses during storage. To identify resistant varieties of maize against this pest, an experiment was conducted in a Completely Randomized Design (CRD) with 11 varieties in free and no-choice conditions. The study measured weight loss, mean bored grain, debris, and weevil numbers at 30, 60, and 90 days. The findings showed that BG13Y-POP, Manakamana-7, and RML-19/RML-6 were the most resistant varieties, with weight loss percentages of 1.99%, 1.47%, and 1.74%, respectively, and final weevil numbers of 104, 72, and 73. Ganesh-2 and ZM-401 were the most susceptible varieties, with weight losses of 7.34% and 6.05%, respectively. The maximum debris weight was found in RML-761/RL-105 (1.98 g), while the minimum was found in Manakamana-7 (0.26 g). The highest number of bored grains was observed in Ganesh-2 (81), while the lowest number was observed in Rampur-4 (51). Similarly, ZM-401 (158) and Ganesh-2 (165) exhibited the highest weevil population, while the lowest count was found in Rampur-4 (72). Overall, using resistant varieties, such as Manakamana-7, BG13Y-POP, and RML-19/RML-6, can be an effective approach for reducing post-harvest losses from weevil infestation.
اظهر المزيد [+] اقل [-]