The effect of antifreeze protein type III (AFPIII) and human heat shock protein 70 (HHSP70), added to the vitrification medium of mouse embryos, on post-freeze/thaw in vitro embryonic growth rates and cell numbers were investigated. In total 20 female mice were synchronized. After synchronization, 2 females and 1 male were mated in the same cage. Twenty-four h after mating, the embryos were collected at the pronuclear stage. In total 8 groups were established, including a positive control group (C+), a negative control group (C-), and treatment groups that were vitrified in a medium added with 200, 400 and 800 ng/ml of AFPIII (AFPIII200, AFPIII400, AFPIII800), and 1, 2 and 4 µg/ml of HHSP70 (HHSP70-1, HHSP70-2, HHSP70-4). Solid surface vitrification (SSV) medium was prepared for the vitrification of the embryos. Once thawed, in vitro development rates of embryos were followed at 24, 48, 72 and 96 h. Four embryos, which progressed to the full blastocyst stage, were taken from each group and stained with the Hoeschst 33258 and propidium iodine (PI) dyes to determine the inner cell mass (ICM), trophectoderm (TE) and total cell numbers. The groups showed statistically significant difference for in vitro embryonic development rates at 48, 72 and 96 h (p

The current study presents a Turkish Truffle Checklist based on literature and a newly discovered species. Within the two divisions, the list includes 104 species belonging to 35 genera and 20 families, as well as their range and Turkish names. Tuber oligospermum was one of them, and it was recorded for the first time in Turkey. A brief description of newly reported species was provided, along with images of their macro and micro-morphology.

Garo people are tribal communities who live in the middle northern part of Bangladesh from time immemorial. The purpose of the research was to identify the reasons, outline, and impact on the relocation of Garo people in the city areas in Bangladesh. Primary data were collected from face-to-face contact through an interview schedule. The findings indicate that poverty, lack of production, unemployment, and security were the main push factors where urban job opportunity, urban life style and social security were pull factors for garo migration. Pattern analysis showed that 69 percent of respondents migrate to the city without a plan. Large farm and family size discourged the unplanned migarion where deteriorate social security influence garo people for unplanned migration. Study also revealed that garo people access to better income, health facility, transportation have increased. In contrast, the involvement of agricultureal activity greatly reduced. Moreover, migrated family maintain their daily requirement for food by purchasing market. Government should take policy for improving the security, income opportunity, and infrastructure for discouraging the unplanned migration from village to town.

Foeniculum vulgare Mill, known as fennel, is a medicinal plant of the Apiaceae family, widely used in traditional Algerian medicine. The aim of this study was to estimate the polyphenols and flavonoids content and to evaluate the antioxidant, the analgesic and the antiulcer activities of aqueous extract of F. vulgare seeds. Quantitative determination of total polyphenols and flavonoids revealed that this extract contained 551.45±0.010 mg gallic acid equivalent/g of dry extract and 284.83±0.008 mg quercetin equivalent/g of dry extract respectively. The study of the gastroprotective effect showed that this extract is able to protect the stomach against lesions induced by 70% ethanol. The percentages of protection were 55.54±6.99 and 71±3.09% for the 200 and 400 mg/kg doses respectively. The study of the analgesic activity indicated that the aqueous extract of F. vulgare reduced the pain induced by acetic acid (0.6%) with an inhibition rate of 47.89% and 68.65% for doses of 200 and 400 mg/kg respectively. Free radical 2,2-diphenyl-1-picrylhydrazyl and iron chelation tests were applied to evaluate the in vitro antioxidant activity. The free radical scavenging activity of F. vulgare extract against 2,2-diphenyl-1-picrylhydrazyl radicals revealed an IC50 value of IC50=30.91±0.49 mg/mL in comparison with gallic acid (0.038±0.0002 mg/mL). The iron chelating test showed that the extract had a high capacity for iron chelating, which was estimated at 0.346±0.003 mg/mL in comparison with the chelating reference agent, ethylene diaminetetraacetic acid.

The storage of spermatozoa and keeping alive for years in the spermatheca by the honey bee queen is a phenomenon allowing her to fertilize eggs throughout her life. In this study, the queens that were 2-week, 1-year, and 2-year-old were analyzed to determine the viability of spermatozoa in their spermathecae. The sister queens reared by the grafting method were instrumentally inseminated with 8 μl fresh semen when they became 6 days old. One week after instrumental insemination, one batch of queens (2-week-old) was dissected for spermatozoa viability test. Another set of queens was introduced into production colonies in Langstroth hives after the onset of oviposition in the mating nuclei. The queens were maintained in production colonies for one year and two years until the dissection process for spermatozoa viability test. The viability of spermatozoa was measured by the dual staining method. We determined that the mean viability of spermatozoa in 2-week, 1-year and 2-year-old queens were 97.3%, 91.1% and 88.1%, respectively. The viability of spermatozoa in queens decreased with age, and the differences between the viability means were significant. However, we did not detect a steep decline in the viability of spermatozoa in queens (6% in one year and 9% in two years) in a wide range of timescale in contrast to previous reports. Furthermore, we found high viability of spermatozoa in the spermathecae of queens at the start of their lives and one- and two-years of age.

Postharvest storage conditions frequently affect nutritional quality of fruits and vegetables. Temperature and storage duration are the most important factors to extend shelf life and maintain quality of fresh watermelon. This study was conducted to determine the changes in the antioxidant properties of watermelon during storage. Fruits of the watermelon cultivars were harvested and stored at 5ºC for a period of 15 days. During storage antioxidant contents (lycopene and total phenolics) and total antioxidant activity were evaluated. The objective of this work was to determine the content of the antioxidant properties in different watermelon genotypes at four different postharvest storage periods (0, 5, 10 and 15 days). Furthermore, the nutritional quality is strongly influenced by the storage period. The cv Mahdia was the most suitable for extended storage periods.

Mechanical properties provide information to design and develop suitable machines (equipment) for processing, transporting, and conveying chestnuts. Four chestnut cultivars that have not been studied before were investigated in the study carried out for this purpose. Some engineering properties of Macit 55, Akyüz, Ali Nihat, and Bouche de Betizac chestnut cultivars were determined and compared. The mechanical properties were determined by rupture force, rupture energy, deformation, and firmness values. The friction coefficients of chestnut varieties on a galvanized sheet, stainless steel, and rubber surfaces were investigated. Mechanical properties were determined using a Universal Testing Machine. The values obtained from the samples were obtained by compression between the parallel plate along the X, Y, and Z axes. For the static friction coefficient, while the galvanized sheet surface had the lowest value (0.145), the rubber surface had the highest value (0.212). For rupture forces, the force required to break the chestnut at the Z loading axis position (714.09 N) was higher than the required force at the Y loading axis position (396.35 N) of the fruit.

During cheese ripening many biochemical reactions occur, including hydrolysis of fat, lactose metabolism, and protein breakdown. The aim of this study is to compare the direct measurement method from cheese with simple extraction methods in determining the hydrolysis of fats by FTIR spectroscopy. For this purpose, white cheese samples were produced, ripened for 60 days and analysed at 20-day intervals. The content of fatty acids was determined by gas chromatography, while its estimation was performed using Partial Least Squares Regression (PLSR). As a result, the PLSR performance obtained from fat extract samples by FTIR spectroscopy was shown higher values than the performance obtained from the direct measurement from cheese. It has been shown that simple extraction methods provide higher performance in FTIR spectroscopic measurements made from cheese.

The purpose of this paper is to look into the determinants of agricultural output growth in Ethiopia. Along with this general objective, this study intends to look at the trend of total factor productivity growth in the agricultural sector and its contribution to agricultural output growth. Using autoregressive distributed lag model bounds testing, this research estimates the long-run and short-run cointegration between agricultural output growth and the total factor productivity. In this study, Augmented Dickey-Fuller and Phillip-Perron unit root tests were used to find out the order of integration of the variables. The selected econometric model goes through all the diagnostic tests and confirms the absence of heteroscedasticity, serial correlation, and normality. The finding of this study indicates that total factor productivity, agricultural land, agricultural machinery, and fertilizer all have a significant and positive effect on the growth of agricultural output. According to the findings of this study, total factor productivity is the primary driver of agricultural output growth. Therefore, as it has played a strong role in developed countries' agriculture, total factor productivity has the potential to be a game-changer in terms of sustainable agricultural growth. Taking into account the findings of this study, we strongly recommend that the government of Ethiopia should devise policies in the agricultural sector that could enhance the level of total factor productivity.