Food fraudulent activities have become a serious issue over the world recently. Seafood products have trading and profitable potential in Turkey owing to the abundance of fisheries and other species. While morphological features are commonly used for species identification in raw seafood products, this identification does not meet the correct classification in cryptic species and processed seafood products. Molecular techniques have been utilized for species authentication in processed seafood items successfully. In this study, the effect of different processing techniques on the DNA quality and DNA degradation isolated from raw and processed anchovy was investigated. Anchovy is one of the important species in both fisheries activities and processing and consumption in Turkey. For this aim, DNA was isolated from processed anchovy groups and un-processed anchovy groups as control by the same extraction methods and the quality of DNA was compared among the groups. The most common processing techniques, frying, baking, smoking, roasting, baking and grilling were applied to anchovy. The results revealed that not only different thermal processing but also treatment with acid and salt cause DNA degradation and quality loss of DNA parameters which are essential for authentication of species and traceability for public health.

The productivity of tomato in Nepal is very low due to lack of high yielding, disease and pests resistant varieties. An experiment was carried out to evaluate horticultural traits of 50 genotypes obtained from World Vegetable Centre (WorldVeg), Taiwan and SAARC region, and local collections during March to August 2020 in open field conditions at National Horticulture Research Centre, Khumaltar, Lalitpur. The objectives were to identify promising open-pollinated tomato cultivars for high yield, appropriate fruit size, and disease resistant. The experiment was carried out in a randomized complete block design with three replications. Results showed significant differences in yield and yield attributing characters including virus infection. The highest yield (39.6 mt ha-1) was produced by HRA43 and it was followed by HRA33 (26.4 mt ha-1). Among the WorldVeg OP lines, AVTO1429 produced the highest yield (16.21 mt ha-1) and it was followed by AVTO1717 (12.95 mt ha-1), AVTO0922 (11.83 mt ha-1) and AVTO1219 (11.7 mt ha-1) respectively. Most of the WorldVeg lines performed better than the check variety ‘Pusa Ruby’. Genotype HRA43, Red Local and Sindhupalchock Local were not affected by virus while Yellow Local showed 3.3% infection. Among the WorldVeg lines, AVTO1712 (20%), AVTO1717 (20%) and AVTO1718 (13%) and AVTO1219 (15%) showed less than 20% virus infection in the open field conditions. Cluster analysis using the unweighted paired group method with arithmetic mean showed that cluster-1 was the largest cluster comprised of 40 genotypes followed by cluster-2 and cluster-4. Genotypes from cluster-4 showed the higher fruit yield (25.1 mt ha-1) and resistant to the virus and the highest number of fruits per plot (1978 in 4.5 m2 area). The yield was low in cluster-1 which could be due to the heavy rainfall during the vegetative and reproductive stages. Genotypes Red Local and Sindhupalchock Local could be used in future tomato breeding program due to their resistant to TYLCV, higher potential yield and highest plant vigour in open field conditions at the mid-hill of Nepal. Considering the overall performance, genotype HRA43, HRA33 and AVTO1429 were promising lines with performance for yield and other horticultural traits.

Ethiopia’s export earning is heavily dependent on primary agricultural commodities and raw materials. Coffee has been the principal export commodity of Ethiopia for many years and continued to be the leading export commodity. The objective of this paper was, therefore, to identify the determinants of Ethiopia’s coffee export to the major trading partners. Eighteen countries were selected based on the importance of the country as Ethiopia’s coffee export destination and availability of the required data. Annual panel data from 2001 to 2016 was collected from FAO database and other relevant sources. After appropriate econometric tests had been applied, random effect model was selected and used to analyze the data. From the seven variables entered into the model, four variables were found to affect Ethiopia’s coffee export significantly. GDP of the importing countries and population size of Ethiopia affect Ethiopia’s coffee export positively as expected. Weighted distance between Ethiopia and its trading partners was also found to have an expected effect, negative, on Ethiopia’s coffee export. Contrary to the hypothesis, foreign direct investment flows to Ethiopia affected Ethiopia’s coffee export negatively. Based on the results, the study draws conclusion and policy implications. To increase Ethiopia’s coffee export, government and other stakeholders should give prime attention to countries where there is high demand for Ethiopia’s coffee. Moreover, coffee exporters should exploit the existing nearest market opportunities. Finally, favourable conditions should be created for the large unemployed labor of the country to increase coffee production and export.

Lumpy skin disease is explained as a significant cattle disease affecting cattle of many parts of the world. An outbreak was experienced in different parts of Bangladesh including Barishal for the first time in the last quarter of 2019. This investigation was carried out to know the outbreak scenario of the disease in southern part of Bangladesh. A total of 726 cattle were included in this study covering 50 dairy farms of Barishal region from September 2019 to December 2019. Morbidity rate was found 21% (CI: 18-24%) and mortality was 1% (CI: 1-2%) in the outbreak area. Young animals (24%) and pregnant animals (70%) were significantly more susceptible compared to aged animals (17%) and non-pregnant animals (15%), respectively. Moreover, male and crossbred cattle were slightly more prone to the infection in comparison to their counterparts. About 45% of the affected animals showed nodular and remaining 55% had edematous lesions. Almost 90% of the affected animals were treated with NSAID followed by antibiotics, antihistaminic, steroid drugs and antiviral drugs where only 20% of the affected animals were treated by authorized veterinary personnel. This was the first epidemiological investigation in the mentioned outbreak area which may serve as a baseline for LSD research in the location needed for developing effective control strategy.

Green tea obtained from the leaves of Camellia sinensis (L.) Kuntze having potential health benefits. It contains significant amounts of antioxidant and is considered as world healthiest drink. Polyphenols in tea are thought to exhibits anti-carcinogenic and anti-inflammatory effects. Green tea catechins acts as immune modulators in immune dysfunction and also play a role in protection from degenerative diseases. Intake of green tea catechins suppress high fat diet induced obesity, prevent lipid and glucose metabolism disorders and reduce risk of coronary heart disease. Green tea constituent epigallocatechin-3-gallate (EGCG) is a potential antioxidant that shows protective effects against photo toxicity and photo carcinogenesis. In vitro animal experiments have suggested that polyphenols in green tea protect from lung cancer by their antioxidant and antimutagenic properties. This study chiefly highlights the convenience of green tea on health and propagates its further prospects.

Bu çalışmada, tıbbi nitelik taşıdığı bilinen Achillea millefolium L. (Civanperçemi) bitkisinin besin elementi konsantrasyonlarının ve antioksidan aktivitesinin belirlenmesi amaçlanmıştır. Bu amaçla; Sivas ili Divriği ilçesinden toplandığı bilinen Civanperçemi bitkisinin makro ve mikro besin elementi konsantrasyonları ile su ekstresinin toplam antioksidan seviyesi (TAS), toplam oksidan seviyesi (TOS) ve oksidatif stres indeksi (OSI) değerleri belirlenmiştir. Çalışmadan elde edilen sonuçlara göre; Civanperçemi bitkisinin bazı makro ve mikro besin elementi konsantrasyonları bakımından yeter düzeyde olduğu, makro elementlerden azot (N), fosfor (P), potasyum (K), kalsiyum (Ca) ve magnezyum, mikro elementlerden demir (Fe), çinko (Zn), mangan (Mn) ve bakır (Cu) konsantrasyonlarının sırasıyla %1,01 N, %0,63 P, %2,43 K, %2,22 Ca, %0,70 Mg, 360,4 mg/kg Fe, 47,6 mg/kg Zn, 85,5 mg/kg Mn ve 28,3 mg/kg Cu olduğu tespit edilmiştir. Antioksidan potansiyelinin ise orta düzeyde olduğu belirlenmiştir. Ayrıca, bitkinin oksidan kapasitesi ve oksidatif stres indeksi düşük olarak tespit edilmiştir.

In this study, it was aimed to report some traits of Gökçeada goats, which raised under the natural conditions of Gökçeada, with respect to their protection within the scope of genetic resources. In this respect the information obtained from the goats supplied from the Island and reared in a semi-intensive system at Çanakkale Onsekiz Mart University and also the information obtained from the literature were collected and the biological and zootechnical identifications of the Gökçeada goat were performed. Gökçeada goats, which are predominantly black, have yellow or red blazes on both sides of their heads including their eyes. It was seen that Gökçeada goats had similar body measurements and lower live weights as compared to those of the same species on the mainland. The birth weight and the mature live weight of Gökçeada goats were found as 2.55 kg and 38 kg, respectively, whereas their mean kid yield per goat at birth was determined as 1.6 to 1.8 kids. Individuals with a milk yield of 591 kg were striking in the Gökçeada genotype, in which the mean lactation length and the mean lactation milk yield were determined as 251 to 259 days and 227 to 245 kg, respectively. Its milk fat (4.92-5.75%) and milk protein (3.29%) resembled those of our other native breeds. It is necessary to reveal, with more elaborate studies, the potential for Gökçeada goat cheese that is greatly demanded on the Island. It was determined that the Gökçeada kids, which were considered as dairy kids, had some small but nonfat carcass and that their meat was soft and of a light color and had a slight smell. One should be careful about the adaptation of the Gökçeada genotype, which successfully maintains itself under the island conditions and which is the source of income for producers, to intensive and extensive goat production systems.

Heavy metal toxicity can either be acute or chronic effects. Long-term exposure of the body to heavy metal can progressively lead to muscular, physical and neurological degenerative process. In this research, a total of 30 representative chocolate samples were collected from local shops and markets in Lahore. All the samples were analysed to assess the levels of Lead (Pb), Nickel (Ni), Chromium (Cr) and Cadmium (Cd) by using Atomic Absorption Spectrophotometer in Pakistan Council of Scientific and Industrial Research (PCSIR), Lahore. It was observed that the majority of chocolate samples contain heavy metals levels higher than the permissible limits as recommended by Punjab Food Rules (PFR) 2011. The levels of Pb ranged between 0.375 and 3.4 mg/kg with 90% samples having concentrations exceeding PFR allowable limit (0.5 mg/kg). Whereas, Ni concentrations varied from 0.005 to 0.28 mg/kg and 80% samples were above the PFR limit (0.025 mg/kg). Further, Cr levels analysed between 0.005 to 0.28 mg/kg and 53% samples had Cr concentrations higher than PFR limit (0.02 mg/kg). Similarly, Cd levels were observed between 0.50-3.25 mg/kg with 53% samples having Cd concentrations exceeding the PFR limit (1.0 mg/kg). Investigation indicates that the quality of available chocolates is not recommendable for eating because of the higher concentrations of toxic heavy metals.

This study was carried out to determine the effects of lactic acid bacteria+ enzyme (LAB+E) inoculants on the fermentation characteristics and feed values of silages prepared from alfalfa harvested at three maturity stages. Alfalfa was harvested at the early, middle and late flowering stages. Sil-All (Alltech, UK) were used as LAB+E inoculants. Inoculants were applied to the silages at the rates of 1×105, 5×105 and 1×106 cfu/g levels in 1 liter capacity plastic bags. The bags were stored at 20±2°C under the laboratory conditions. Three bags from each group were sampled for chemical and microbiological analyses on the 45th day after ensiling. The results showed that LAB+E inoculants reduced pH values and ammonia-nitrogen content, whereas increased lactic acid contents and lactobacillus count of alfalfa silages. High doses LAB+E inoculant decreased neutral detergent fiber and acid detergent fiber content, increased in vitro organic matter digestibility and metabolic energy of alfalfa silages. It has been demonstrated that the most effective application dose of LAB+E inoculant to improve fermentation and feed value of alfalfa silage was 1×106 cfu/g, but 1x105 and 5×105 cfu/g level can also be considered as effective dose.