The efficiency of biological products combined with biological fertilization for controlling Pratylenchus brachyurus in soybean crops, and effects of these treatments on soybean crops were evaluate. A greenhouse experiment was conducted at the Mato Grosso State University, in Brazil, using a randomized block design with a 10×2 factorial arrangement, and four replications. The treatments consisted of Trichoderma asperellum, B. subtilis, Purpureocillium lilacinum, B. subtilis + T. asperellum, B. subtilis + P. lilacinum, T. asperellum + P. lilacinum, B. subtilis + T. asperellum + P. lilacinum and abamectin, and Controls with, and without nematodes. Nematode population, plant height, stem base diameter, and soil microbiological characteristics (at 60 and 120 days after sowing - DAS), shoot dry weight (60 DAS), and number of pods per plant, and grain yield (120 DAS) were evaluated. The treatments were efficient for the control of phytonematodes at 60 DAS; the efficiency of treatments with biological products increased when combined with biological fertilization. The biological products were more efficient for controlling the nematodes than abamectin at 120 DAS; and the percentage of control were higher when they were combined with biological fertilization, in both growing periods. The biological treatments resulted in better agronomic characteristics, and higher number of pods per plant, and grain yield, affecting the plants in the first growing period, and significant interaction with the biological fertilizer for these variables in the second growing period. The interaction between treatments and biological fertilization was significant for soil microbiological characteristics in the second growing period. | Objetivou-se avaliar a eficiência de produtos biológicos associados à adubação biológica no biocontrole de Pratylenchus brachyurus na cultura da soja, além do impacto destestratamentos sobre o desenvolvimento da cultura. O experimento foi conduzido em casa de vegetação, na UNEMAT, Campus de Tangará da Serra, com delineamento experimental em blocos casualizados, esquema fatorial 10X2, com quatro repetições. Foram avaliados: testemunha com e sem nematoides, Trichoderma asperellum, Bacillus subtilis, Purpureocillium lilacinum, B. subtilis+ T. asperellum, B. subtilis+ P. lilacinum, T. asperellum+ P. lilacinum, B. subtilis+ T. asperellum+ P. lilacinum e abamectina. Avaliou-se o nível populacional de P. brachyurus, altura, diâmetro do colo das plantas e características microbiológicas do solo aos 60 e 120 DAS; massa seca da parte aérea aos 60 DAS; número de vagens/planta e produtividade aos 120 DAS. Observou-se que aos 60 DAS os tratamentos promoveram eficiência no controle do fitonematoides, quando associados a adubação biológica somente os tratamentos biológicos melhoraram sua eficiência. Aos 120 DAS os produtos biológicos apresentaram maior eficiência no controle de P. brachyurus que a abamectina, aumentando sua eficiência quando adicionada a adubação biológica (nas duas épocas de semeadura). As características agronômicas, número de vagens/planta e produtividade submetidas aos tratamentos biológicos apresentaram melhor desempenho, havendo interação significativa entre tais tratamentos na primeira época de semeadura e interação com à aplicação do adubo biológico para estas variedades nas segunda época de semeadura. Com relação ás características microbiológicas do solo, houve interação entre os tratamentos somente com a aplicação da adubação biológica na segunda época de semeadura.

The efficiency of biological products combined with biological fertilization for controlling Pratylenchus brachyurus in soybean crops, and effects of these treatments on soybean crops were evaluate. A greenhouse experiment was conducted at the Mato Grosso State University, in Brazil, using a randomized block design with a 10×2 factorial arrangement, and four replications. The treatments consisted of Trichoderma asperellum, B. subtilis, Purpureocillium lilacinum, B. subtilis + T. asperellum, B. subtilis + P. lilacinum, T. asperellum + P. lilacinum, B. subtilis + T. asperellum + P. lilacinum and abamectin, and Controls with, and without nematodes. Nematode population, plant height, stem base diameter, and soil microbiological characteristics (at 60 and 120 days after sowing - DAS), shoot dry weight (60 DAS), and number of pods per plant, and grain yield (120 DAS) were evaluated. The treatments were efficient for the control of phytonematodes at 60 DAS; the efficiency of treatments with biological products increased when combined with biological fertilization. The biological products were more efficient for controlling the nematodes than abamectin at 120 DAS; and the percentage of control were higher when they were combined with biological fertilization, in both growing periods. The biological treatments resulted in better agronomic characteristics, and higher number of pods per plant, and grain yield, affecting the plants in the first growing period, and significant interaction with the biological fertilizer for these variables in the second growing period. The interaction between treatments and biological fertilization was significant for soil microbiological characteristics in the second growing period.

Dwarf coconut tree is the main variety for commercial use in Brazil, which ranks fourth in world coconut production. However, the genotypes used still have limitations and genetic variability is required. The aim of this study was to estimate the genetic variability in dwarf coconut accessions preserved at the Germplasm Bank of Brazil at different harvesting times and using agronomic descriptors of plant and fruits. The accessions Brazilian Green Dwarf-Jiqui, Cameroon Red Dwarf, Malayan Red Dwarf, Brazilian Red Dwarf-Gramame, Brazilian Yellow Dwarf-Gramame, and Malayan Yellow Dwarf were assessed by means of 30 descriptors Variance analysis was performed and the genetic diversity was quantified by using the Mahalanobis’ generalized distance and expressed by means of UPGMA clusters, Tocher optimization, and canonical variables. The maximum likelihood analysis was used to estimate the components of variance with the data of each plant in a sample of 11 descriptors of great importance for the genetic improvement of the coconut tree. A phenotypic divergence was found among the accessions using the UPGMA clusters, Tocher optimization and graphic dispersion obtained with canonical variables. The use of the maximum likelihood analysis confirms the existence of genetic variability in the accessions for the descriptors fruit polar and equatorial diameter, nut polar diameter, total fruit weight, and epicarp thickness, which presented a heritability varying from 0.17 to 0.39. There is a possibility of genetic gains with the selection of these traits for use of accessions in breeding programs. | O coqueiro anão é a principal variedade para uso comercial no Brasil, que ocupa atualmente a quarta posição na produção mundial. No entanto, os genótipos utilizados no país ainda apresentam limitações e há necessidade de variabilidade genética.  Este trabalho teve por objetivo estimar a variabilidade genética em acessos de coqueiro-anão conservados no Banco de Germoplasma existente no Brasil, em diferentes épocas de colheita, utilizando descritores agronômicos de planta e frutos. Os acessos anão-verde-do-Brasil-de-Jiqui; anão-vermelho-de-Camarões; anão-vermelho-da-Malásia; anão-vermelho-de-Gramame; anão-amarelo-de-Gramame e anão-amarelo-da-Malásia foram avaliados por meio de 30 descritores. Análise de variância foi realizada e a  diversidade genética foi quantificada utilizando a distância generalizada de Mahalanobis e expressa por meio de agrupamentos UPGMA, otimização de Tocher e variáveis canônicas.  A análise de máxima verossimilhança foi utilizada para estimar os componentes de variância com os dados de cada planta em uma amostra de 11 descritores de maior importância para o melhoramento genético do coqueiro. Foi encontrada divergência fenotípica entre os acessos usando os agrupamentos UPGMA, Tocher e a dispersão gráfica obtida com variáveis canônicas. O emprego da análise de máxima verossimilhança confirma a existência de variabilidade genética nos acessos para os descritores diâmetro polar e equatorial do fruto, diâmetro polar da noz, peso total do fruto e espessura de epicarpo que apresentaram herdabilidade variando de 0,17 a 0,39. Há possibilidade de ganhos genéticos com a seleção desses caracteres para uso dos acessos em programas de melhoramento genético.

Dwarf coconut tree is the main variety for commercial use in Brazil, which ranks fourth in world coconut production. However, the genotypes used still have limitations and genetic variability is required. The aim of this study was to estimate the genetic variability in dwarf coconut accessions preserved at the Germplasm Bank of Brazil at different harvesting times and using agronomic descriptors of plant and fruits. The accessions Brazilian Green Dwarf-Jiqui, Cameroon Red Dwarf, Malayan Red Dwarf, Brazilian Red Dwarf-Gramame, Brazilian Yellow Dwarf-Gramame, and Malayan Yellow Dwarf were assessed by means of 30 descriptors Variance analysis was performed and the genetic diversity was quantified by using the Mahalanobis’ generalized distance and expressed by means of UPGMA clusters, Tocher optimization, and canonical variables. The maximum likelihood analysis was used to estimate the components of variance with the data of each plant in a sample of 11 descriptors of great importance for the genetic improvement of the coconut tree. A phenotypic divergence was found among the accessions using the UPGMA clusters, Tocher optimization and graphic dispersion obtained with canonical variables. The use of the maximum likelihood analysis confirms the existence of genetic variability in the accessions for the descriptors fruit polar and equatorial diameter, nut polar diameter, total fruit weight, and epicarp thickness, which presented a heritability varying from 0.17 to 0.39. There is a possibility of genetic gains with the selection of these traits for use of accessions in breeding programs.

Arbuscular mycorrhizal fungi (AMF) and growth promoting bacteria in plants (PGPBs) benefit the survival and development of plantlets; such benefits are attributed to the increased absorption of nutrients, increased photosynthetic rate and tolerance to abiotic stress. The aim of this study was to evaluate if the co-inoculation PGPBs and the AMF, Glomus clarum, improves the growth of micropropagated Manihot esculenta Crantz. The experiment was conducted in the greenhouse. The PGPBs used were Azospirillum amazonense (BR 11140), Herbaspirillum seropedicae (BR 11175), Gluconacetobacter diazotrophicus (BR 11284) and Streptomyces sp. (S 30) and mixtures with double inoculation (Streptomyces sp + A. amazonense, Streptomyces sp + H. seropedicae, Streptomyces sp + G. diazotrophicus, A. amazonense + H. seropedicae, A. amazonense + G. diazotrophicus, and H. seropedicae + G. diazotrophicus). Strains of PGPBs inoculated in cassava cv. "BRA Pretinha III" influenced mycorrhizal colonization and the number of glomerospores, and synergistic effects occurred between Glomus clarum and PGPBs. The crude protein content revealed the contribution of PGPBs to nitrogen nutrition in cassava where the inoculated plants assimilated N in equal proportion with those that received mineral nitrogen. The combined inoculation of PGPBs in the presence of Glomus clarum was significant in cassava and fostered better performance in plant growth over time, similar to all the variables studied. Co-inoculation of PGPBs and AMF can meet the N need of cassava, with implications for the reduced use of N fertilizer. | Os fungos micorrízicos arbusculares (FMA) e com as bactérias promotoras de crescimento (BPCPs) beneficiam a sobrevivência e o desenvolvimento de plântulas; tais benefícios são atribuídos ao aumento da absorção de nutrientes, aumento da taxa fotossintética, e aumento da tolerância a estresse abiótico. O objetivo deste estudo foi o de avaliar se a co-inoculação de BPCPs e do FMA, Glomus clarum, melhora o crescimento de Manihot esculenta Crantz micropropagada. As BPCPs utilizadas foram Azospirillum amazonense (BR 11140), Herbaspirillum seropedicae (BR 11175), Gluconacetobacter diazotrophicus (BR 11284) e Streptomyces sp. (S 30), isoladas e em mistura com dupla inoculação (Streptomyces sp. + A. amazonense), (Streptomyces sp. + H. seropedicae), (Streptomyces sp. + G. diazotrophicus), (A. amazonense + H. seropedicae), (A. amazonense + G. diazotrophicus), (H. seropedicae + G. diazotrophicus). As estirpes de BPCPs inoculadas na mandioca cv. "BRA Pretinha III" influenciaram a colonização micorrízica e o número de glomerosporos. Nessas  condições ocorreram efeitos sinérgicos de Glomus clarum com essas BPCPs. O teor de proteína bruta revelou a contribuição de BPCPs na nutrição nitrogenada de mandioca em que  as plantas inoculadas assimilaram N em proporção igual àquelas que receberam nitrogênio mineral. A inoculação combinada de BPCPs na presença de Glomus clarum foi significativa na mandioca e melhorou o crescimento da planta ao longo do tempo, como em todas as variáveis estudadas. A co-inoculação de BPCPs e FMA pode atender a necessidade de N para mandioca, implicando redução no uso de fertilizante nitrogenado.

Arbuscular mycorrhizal fungi (AMF) and growth promoting bacteria in plants (PGPBs) benefit the survival and development of plantlets; such benefits are attributed to the increased absorption of nutrients, increased photosynthetic rate and tolerance to abiotic stress. The aim of this study was to evaluate if the co-inoculation PGPBs and the AMF, Glomus clarum, improves the growth of micropropagated Manihot esculenta Crantz. The experiment was conducted in the greenhouse. The PGPBs used were Azospirillum amazonense (BR 11140), Herbaspirillum seropedicae (BR 11175), Gluconacetobacter diazotrophicus (BR 11284) and Streptomyces sp. (S 30) and mixtures with double inoculation (Streptomyces sp + A. amazonense, Streptomyces sp + H. seropedicae, Streptomyces sp + G. diazotrophicus, A. amazonense + H. seropedicae, A. amazonense + G. diazotrophicus, and H. seropedicae + G. diazotrophicus). Strains of PGPBs inoculated in cassava cv. "BRA Pretinha III" influenced mycorrhizal colonization and the number of glomerospores, and synergistic effects occurred between Glomus clarum and PGPBs. The crude protein content revealed the contribution of PGPBs to nitrogen nutrition in cassava where the inoculated plants assimilated N in equal proportion with those that received mineral nitrogen. The combined inoculation of PGPBs in the presence of Glomus clarum was significant in cassava and fostered better performance in plant growth over time, similar to all the variables studied. Co-inoculation of PGPBs and AMF can meet the N need of cassava, with implications for the reduced use of N fertilizer.

Intercropping system and the use of green manure with spontaneous species has been an alternative farming method applied to the productive sector of vegetables in the northeastern semi-arid region. The objective of this work was to determine which amount of Calotropis procera must be incorporated into the soil to provide the highest productive performance of the component crops and increase the profitability of the carrot and cowpea intercropping. The experimental design used was randomized complete blocks with five replicates. The treatments consisted of four amounts of C. procera incorporated into the soil: 10, 25, 40 and 55 t ha-1 on a dry basis. The characteristics evaluated in this intercropping system were: commercial productivity of carrot roots, yield of cowpea green grains, land equivalent ratios for component crops and for the  intercropped system, score of the canonical variable of the association, and the economic indicators of gross income, net income, rate of return, and net profit margin. The maximum agronomic efficiency of the carrot x cowpea intercropping was reached at the land equivalent ratio of 1.12, using 43.39 t ha-1 of C. procera biomass incorporated in the soil, while the maximum economic efficiency of the carrot and cowpea crops association was obtained at the net income of R$ 17,856.43 ha-1, in the amount of 40.60 t ha-1 of C. procera biomass added to the soil. | Sistema consorciado e uso de adubação verde com espécies espontâneas tem sido um método alternativo de cultivo aplicado ao setor produtivo de hortaliças na região semiárida nordestina. O objetivo deste trabalho foi determinar qual quantidade de Calotropis procera deve ser incorporada ao solo para proporcionar o melhor desempenho produtivo das culturas componentes e aumentar a rentabilidade do consórcio de cenoura e caupi. O delineamento experimental utilizado foi de blocos completos casualizados com cinco repetições. Os tratamentos consistiram de quatro quantidades de C. procera incorporadas ao solo: 10, 25, 40 e 55 t ha-1, em base seca. As características avaliadas neste sistema consorciado foram: produtividade comercial de raízes de cenoura, produtividade de grãos verdes de caupi, índices de uso eficiente da terra das culturas componentes e do consórcio, escore da variável canônica da associação e os indicadores econômicos renda bruta, renda líquida, taxa de retorno e margem de lucro líquido. A máxima eficiência agronômica do consórcio cenoura x caupi foi alcançada no índice de uso eficiente da terra de 1,12, utilizando-se 43,39 t ha-1 de biomassa de C. procera incorporada ao solo, enquanto a eficiência econômica máxima da associação cenoura x caupi foi obtida com a renda líquida de R$ 17.856,43 ha-1, na quantidade de 40,60 t ha-1 de biomassa de C. procera adicionada ao solo.

Intercropping system and the use of green manure with spontaneous species has been an alternative farming method applied to the productive sector of vegetables in the northeastern semi-arid region. The objective of this work was to determine which amount of Calotropis procera must be incorporated into the soil to provide the highest productive performance of the component crops and increase the profitability of the carrot and cowpea intercropping. The experimental design used was randomized complete blocks with five replicates. The treatments consisted of four amounts of C. procera incorporated into the soil: 10, 25, 40 and 55 t ha-1 on a dry basis. The characteristics evaluated in this intercropping system were: commercial productivity of carrot roots, yield of cowpea green grains, land equivalent ratios for component crops and for the intercropped system, score of the canonical variable of the association, and the economic indicators of gross income, net income, rate of return, and net profit margin. The maximum agronomic efficiency of the carrot x cowpea intercropping was reached at the land equivalent ratio of 1.12, using 43.39 t ha-1 of C. procera biomass incorporated in the soil, while the maximum economic efficiency of the carrot and cowpea crops association was obtained at the net income of R$ 17,856.43 ha-1, in the amount of 40.60 t ha-1 of C. procera biomass added to the soil.

Brave bean (Capparis flexuosa L.) is a Caatinga species that is used as forage, mainly during the dry season when some plant species lose their leaves. The aim of this study was to assess genetic diversity within and among brave bean populations using Random Amplified Polymorphic DNA (RAPD) markers. Brave bean leaves were collected from 30 accessions in the following municipalities of Paraíba state, Brazil: Barra de Santa Rosa (BSR), Cuité (C), São João do Cariri (SJC), Damião (D), Baraúna (B), and Picuí (P). DNA extraction followed the standard methodology of CTAB with modifications. RAPD analyses were carried out using 18 primers, and polymorphism of the amplified DNA fragments was visualized using agarose gel electrophoresis. Data were used to calculate Jaccard Similarity Coefficient values, which were then used to group samples with the Unweighted Pair Group Method with Arithmetic Mean. Cophenetic Correlation Coefficient, Stress, and Distortion Coefficient values were also calculated from these analyses. Band polymorphism was generated with 14 primers, but the sampled populations showed low numbers of polymorphic loci (27 in BSR, 18 in C, 7 in SJC, 9 in D, and 0 in B and P). The highest polymorphic information content was found in samples from the BSR (9 groups), C (22 groups), SJC (7 groups), and D (6 groups) municipalities. In the interpopulation analysis, 34 groups were formed, the matrices of which showed high cophenetic correlations (0.95 to 0.98), but low stress (12.9 to 17.45%) and distortion (3.05%). Therefore, results showed that there was genetic variability both among and within brave bean populations. | O feijão-bravo (Capparis flexuosa) é uma espécie nativa da Caatinga e é utilizada como forrageira, principalmente na estação seca onde parte das espécies vegetais perdem suas folhas. Objetivou-se estudar a diversidade genética dentro e entre populações de feijão-bravo usando marcadores RAPD. O estudo foi realizado no Laboratório de Biotecnologia Vegetal do CCA/UFPB. Foram coletadas folhas de 30 acessos de feijão-bravo/população nos seguintes municípios do estado da Paraíba: Barra de Santa Rosa (BSR), Cuité (C), São João do Cariri (SJC), Damião (D), Baraúna (B) e Picuí (P). A extração de DNA seguiu a metodologia de utilização do CTAB com modificações. Análises de RAPD foram realizadas usando 18 primers e o polimorfismo de fragmentos de DNA amplificados foi visualizado em gel de agarose após eletroforese. Os dados foram submetidos ao Coeficiente de Similaridade Jaccard, agrupados seguindo a UPGMA e calculou-se o Coeficiente de Correlação Cofenética, Estresse e Distorção. 14 primers geraram polimorfismo de banda, no entanto, as populações apresentaram baixo número de locos polimórficos (27 em BSR, 18 em C, 7 em SJC, 9 em D, 0 em B e P) sendo o maior conteúdo de informação polimórfica foi encontrado nos municípios de BSR (9 grupos), C (22 grupos), São João do Cariri (7 grupos) e D (6 grupos). Formou-se 34 grupos na análise interpopulacional. As matrizes formadas apresentaram altos índices de correlação cofenética (0,95 a 0,98), baixo estresse (12,9 a 17,45%) e distorção (3,05%). Portanto, há variabilidade genética entre e dentro de populações de feijão-bravo.

Brave bean (Capparis flexuosa L.) is a Caatinga species that is used as forage, mainly during the dry season when some plant species lose their leaves. The aim of this study was to assess genetic diversity within and among brave bean populations using Random Amplified Polymorphic DNA (RAPD) markers. Brave bean leaves were collected from 30 accessions in the following municipalities of Paraíba state, Brazil: Barra de Santa Rosa (BSR), Cuité (C), São João do Cariri (SJC), Damião (D), Baraúna (B), and Picuí (P). DNA extraction followed the standard methodology of CTAB with modifications. RAPD analyses were carried out using 18 primers, and polymorphism of the amplified DNA fragments was visualized using agarose gel electrophoresis. Data were used to calculate Jaccard Similarity Coefficient values, which were then used to group samples with the Unweighted Pair Group Method with Arithmetic Mean. Cophenetic Correlation Coefficient, Stress, and Distortion Coefficient values were also calculated from these analyses. Band polymorphism was generated with 14 primers, but the sampled populations showed low numbers of polymorphic loci (27 in BSR, 18 in C, 7 in SJC, 9 in D, and 0 in B and P). The highest polymorphic information content was found in samples from the BSR (9 groups), C (22 groups), SJC (7 groups), and D (6 groups) municipalities. In the interpopulation analysis, 34 groups were formed, the matrices of which showed high cophenetic correlations (0.95 to 0.98), but low stress (12.9 to 17.45%) and distortion (3.05%). Therefore, results showed that there was genetic variability both among and within brave bean populations.

The presence of microorganisms, especially during storage, can lead to the rapid deterioration of macauba fruits (Acrocomia aculeata) and reduce the quality of extracted oil for biodiesel production. An alternative to ensure the quality of the oil is the use of ozone gas, a oxidizing agent that has high antimicrobial and sanitizing power. The objective of this study was to evaluate the influence of high concentrations of ozone gas on macaúba fruit and its effect on the quality of the oil throughout storage for the production of biodiesel. Ozonation was performed 20 days after harvest using fruits with and without the epicarp. Ozone was applied at a concentration of 18.0 mg L-1 and a flow of 1.5 L min-1 for 10 h. After ozonation, the fruits were stored for different periods, and the physical-chemical parameters of the oil were evaluated. The ozonation of fruits without the epicarp visually reduced microorganism attack. In general, the ozonation process of macauba fruits maintained the visual characteristics of the fruits and the physical-chemical characteristics of the oil for biodiesel production compared to untreated fruits. The average acidity values of the oil were lower in the treatments with the ozone gas. Removal of the epicarp tends to facilitate the ozonation process, potentially by improving gas penetration into the fruit. | A presença de micro-organismo, especialmente durante o armazenamento, pode levar à deterioração rápida dos frutos de macaúba (Acrocomia aculeata) e reduzir a qualidade do óleo extraído para a produção de biodiesel. Uma alternativa para garantir a qualidade do óleo é o uso de gás ozônio, um poderoso agente oxidante que possui alto poder antimicrobiano e sanitizante. O objetivo deste estudo foi avaliar a influência do ozônio gasoso em alta concentração (18,0 mg L-1) em frutos de macaúba (com e sem epicarpo) e seu efeito sobre a qualidade do óleo durante todo o armazenamento para a produção de biodiesel. A ozonização foi realizada 20 dias após a colheita, utilizando frutas com e sem epicarpo. O ozônio foi aplicado na concentração de 18,0 mg L-1 e fluxo de 1,5 L min-1 durante 10 h. Após a ozonização, os frutos foram armazenados por diferentes períodos e foram avaliados os parâmetros físico-químicos do óleo. A ozonização de frutos sem epicarpo reduziu visualmente o ataque de micro-organismos. Em geral, o processo de ozonização de frutos de macauba manteve as características visuais dos frutos e físico-químicas do óleo para produção de biodiesel em relação ao controle não tratado. Os valores médios de acidez do óleo foram menores nos tratamentos com o gás ozônio. A remoção do epicarpo tende a facilitar o processo de ozonização, já que a retirada pode facilitar a penetração do gás no fruto.

The presence of microorganisms, especially during storage, can lead to the rapid deterioration of macauba fruits (Acrocomia aculeata) and reduce the quality of extracted oil for biodiesel production. An alternative to ensure the quality of the oil is the use of ozone gas, a oxidizing agent that has high antimicrobial and sanitizing power. The objective of this study was to evaluate the influence of high concentrations of ozone gas on macaúba fruit and its effect on the quality of the oil throughout storage for the production of biodiesel. Ozonation was performed 20 days after harvest using fruits with and without the epicarp. Ozone was applied at a concentration of 18.0 mg L-1 and a flow of 1.5 L min-1 for 10 h. After ozonation, the fruits were stored for different periods, and the physical-chemical parameters of the oil were evaluated. The ozonation of fruits without the epicarp visually reduced microorganism attack. In general, the ozonation process of macauba fruits maintained the visual characteristics of the fruits and the physical-chemical characteristics of the oil for biodiesel production compared to untreated fruits. The average acidity values of the oil were lower in the treatments with the ozone gas. Removal of the epicarp tends to facilitate the ozonation process, potentially by improving gas penetration into the fruit.

Nitrogen fertilization is a limiting factor for grapevine production; its excess or deficiency can cause changes in fruit quantity and quality. Thus, the objective of this work was to evaluate productive parameters of grapevines of the Isabel-Precoce cultivar subjected to nitrogen and organic fertilization in the western semiarid region of the state of Rio Grande do Norte (RN), Brazil. The experiment was conducted at the Experimental Farm of the Federal Rural University of the Semi-Arid Region, in Mossoró, RN, Brazil, during two production cycles. The soil of the area used was classified as dystrophic Red Argissolo (Udult) of sandy texture. The experiment was conducted in a randomized block design with six replications, using a 5×2 factorial arrangement, with 5 nitrogen fertilizer rates (0, 30, 60, 90, and 120 kg ha-1) and two organic fertilizer rates (0, and 20 m3 ha-1 of bovine manure). The production components number of bunches per plant, grape production per plant, grape yield, weight, length and width of bunches, number of berries per bunch, and ten-berry weight were evaluated. The nitrogen rates and organic fertilizer rates used were significant (p≤0.05) for all evaluated components. The N rate of 60 kg ha-1 combined with the organic fertilization generated the best results for the analyzed variables. N rates from 60 to 120 kg ha-1, applied singly or combined with organic fertilizer, hindered the production components of the Isabel-Precoce grapevines. | A adubação nitrogenada na videira apresenta-se como um fator limitante na produção, visto que seu excesso ou deficiência são capazes de propiciar alterações na quantidade e qualidade final dos frutos. Assim, o objetivo deste trabalho foi avaliar os parâmetros produtivos da videira ‘Isabel Precoce’ submetida a adubação nitrogenada e orgânica na região semiárida do Oeste Potiguar. O experimento foi implantado na Fazenda Experimental da UFERSA, em Mossoró, RN, cujo clima é semiárido. O solo da área é um Argissolo Vermelho Distrófico de textura arenosa. A pesquisa foi realizada em dois ciclos de produção. O delineamento experimental utilizado foi blocos casualizados com seis repetições, em esquema fatorial 5 x 2, que corresponderam a 5 doses de fertilizante nitrogenado (0, 30, 60, 90 e 120 kg ha-1), na ausência e presença de adubação orgânica (0 e 20 m3 ha-1 esterco bovino). Foram avaliados número de cachos por planta, produção por planta e produtividade, bem como peso, comprimento e largura dos cachos, número de bagas por cacho e peso de dez bagas. A aplicação das doses de nitrogênio (N), bem como o uso ou não de adubo orgânico (AO) no solo, conferiram efeito significativo (p≤0,05) para todos os componentes avaliados. A dose de 60 kg ha-1 de N na presença do AO foi a que proporcionou os melhores resultados nas variáveis analisadas. A elevação dos níveis de N, a partir da dose de 60 até 120 kg ha-1 combinadas ou não a fonte orgânica, inibiram os componentes produtivos da videira ´Isabel Precoce´.

Nitrogen fertilization is a limiting factor for grapevine production; its excess or deficiency can cause changes in fruit quantity and quality. Thus, the objective of this work was to evaluate productive parameters of grapevines of the Isabel-Precoce cultivar subjected to nitrogen and organic fertilization in the western semiarid region of the state of Rio Grande do Norte (RN), Brazil. The experiment was conducted at the Experimental Farm of the Federal Rural University of the Semi-Arid Region, in Mossoró, RN, Brazil, during two production cycles. The soil of the area used was classified as dystrophic Red Argissolo (Udult) of sandy texture. The experiment was conducted in a randomized block design with six replications, using a 5×2 factorial arrangement, with 5 nitrogen fertilizer rates (0, 30, 60, 90, and 120 kg ha-1) and two organic fertilizer rates (0, and 20 m3 ha-1 of bovine manure). The production components number of bunches per plant, grape production per plant, grape yield, weight, length and width of bunches, number of berries per bunch, and ten-berry weight were evaluated. The nitrogen rates and organic fertilizer rates used were significant (p=0.05) for all evaluated components. The N rate of 60 kg ha-1 combined with the organic fertilization generated the best results for the analyzed variables. N rates from 60 to 120 kg ha-1, applied singly or combined with organic fertilizer, hindered the production components of the Isabel-Precoce grapevines.