Producción Científica | This work discusses hydrolysis of defatted grape in supercritical water (SCW) at 380 °C and 260 bar from 0.18 s to 1 s focusing attention to sugars recovery in the liquid phase of the product and detailed characterization of remaining solid phase enriched in polyaromatics (e.g. lignin, flavonoids, etc.). After the longest reaction time of 1 s, 56% of carbohydrates could be recovered in the liquid phase, as a result of carbohydrate hydrolysis. The high content of insoluble lignin in biomass (36%), acts as a mass transfer limitation and presents an important feature in the hydrolysis process, slowing down the conversion of carbohydrate fraction, as after the maximum time of 1s, 10% of carbohydrates still remained in the solid phase. Milled wood lignin, extracted from biomass and dioxane extract from the solid phase were characterized in order to understand the main structural changes during the SCW hydrolysis process. Dioxane (80%) extraction of solids produces a very complex mixture of lipophilic extractives, flavonoids and lignin with a certain amount of chemically linked carbohydrates. 2D NMR analysis of dioxane extract shows remarkably subtle changes in the amounts of main lignin moieties (β-O-4′, β-β’ (resinol) and β-5 (phenylcoumaran)). This subtle change of the main lignin structures is an important feature in the further valorisation of this sulfur-free lignin residue. | Ministerio de Ciencia, Innovación y Universidades - Fondo Europeo de Desarrollo Regional (projects CTQ2016-79777-R and PID2019-105975 GB-I00) | Junta de Castilla y León - Fondo Europeo de Desarrollo Regional (project VA277P18)

El objetivo de esta investigación es determinar el efecto del tipo de alimento -uno vivo, representado por Daphnia pulex y uno balanceado de la marca Purina Camaronina 35 con 35% de proteínas- y la calidad del agua -presencia y ausencia de microorganismos, y sólidos suspendidos- en el crecimiento y sobrevivencia de postlarvas del crustáceo Cambarellus montezumae. Para lo cual, se formaron 4 tratamientos que se evaluaron durante 14 semanas, mediante la realización de observaciones y biometrías periódicas como seguimiento del desarrollo en los organismos. Los datos obtenidos registraron diferencias significativas entre tratamientos (p< 0.05), en donde los mejores resultados de crecimiento y sobrevivencia se obtuvieron en los organismos alimentados con el alimento balanceado (M2 y M4), independientemente de la calidad del agua. Además, se obtuvieron valores de proteínas de hasta 45% y en la fracción lipídica se identificaron los ácidos grasos 20:4 n6 (ARA), 20:5 n3 (EPA) y 22:6 n3 (DHA) al considerar el cuerpo total del organismo. Lo antes mencionado incrementa el interés por el cultivo sustentable de C. montezumae para ser utilizado en la industria alimentaria a través de su adición en productos alimentarios de consumo humano debido a su contenido de biomoléculas de importancia biológica. | The objective of this research was to determine the effect of the type of food -a line one represented by Daphnia pulex and a one commercial Camaronina 35 by Purina with 35% protein- and water quality -presence and absence of microorganisms and suspended solids- on growth and survival of postlarvae of the crustacean Cambarellus montezumae. Four treatments were evaluated for 14 weeks conducting observations and periodic measurements as a follow-up of the development of the organisms. The obtained data showed significant differences between treatments (p< 0.05), where the best results in growth and survival were obtained in the organisms fed on animal feed (M2 and M4) regardless of the water quality. In addition, values of up to 45% protein were obtained and the lipid fraction identified as the fatty acids 20:4 n6 (ARA), 20:5 n3 (EPA) and 22:6 n3 (DHA) when considering the total body of the organisms. Our results increase the interest in the sustainable culture of C. montezumae to be used in the food industry through its addition to food products for human consumption due to its content of biomolecules of biological importance.