We quantify the potential impacts of global food production on freshwater availability (water scarcity footprint; WSF) by applying the water unavailability factor (fwua) as a characterization factor and a global water resource model based on life cycle impact assessment (LCIA). Each water source, including rainfall, surface water, and groundwater, has a distinct fwua that is estimated based on the renewability rate of each geographical water cycle. The aggregated consumptive water use level for food production (water footprint inventory; WI) was found to be 4344 km3/year, and the calculated global total WSF was 18,031 km3 H<inf>2</inf>Oeq/year, when considering the difference in water sources. According to the fwua concept, which is based on the land area required to obtain a unit volume of water from each source, the calculated annual impact can also be represented as 98.5 × 106 km2. This value implies that current agricultural activities requires a land area that is over six times larger than global total cropland. We also present the net import of the WI and WSF, highlighting the importance of quantitative assessments for utilizing global water resources to achieve sustainable water use globally.

Optimal allocation of available water in a middle Himalayan watershed by a linear programming model for maximizing production from crops and livestock, after meeting the present and future demands of human and environmental flows, was analyzed. Present and future water availability under different environmental scenarios at various locations in the watershed was considered. Rice Equivalent Production from the watershed was found to improve by 207% (to 919 tonnes) and Rice Equivalent Yield by 58% (to 4427 kg ha⁻¹) through optimal allocation of available water resource. Occurrence of drought of 60% intensity would limit production to 737 tonnes. Environmental degradation by 2025 would though reduce production marginally, the surplus water available within the watershed would, however, decrease significantly during January to March. Future competition for water would adversely affect economy of the watershed and the region. The government should, therefore, undertake water resource development programmes after making a proper inventory of available resources at watershed level by analyzing the current and future supply and demand scenarios.

²H/¹H ratios in animal biomass reflect isotopic input from food and water. A 10-week controlled laboratory study raised 48 mice divided in two generations (8 mothers Mus musculus and their offspring). The mice were divided into four groups based on the combination of ²H, ¹³C, ¹⁵N-enriched and non-enriched food and water. Glycine, the most common amino acid in bone collagen, carried the ²H, ¹³C, ¹⁵N-isotopic spike in food. ANOVA data analysis indicated that hydrogen in food accounted for ∼81 % of the hydrogen isotope inventory in collagen whereas drinking water hydrogen contributed ∼17 %. Air humidity contributed an unspecified amount. Additionally, we monitored ¹³C and ¹⁵N-enrichment in bone collagen and found strong linear correlations with the ²H-enrichment. The experiments with food and water indicate two biosynthetic pathways, namely (i) de novo creation of non-essential amino acids using hydrogen from water, and (ii) the integration of essential and non-essential amino acids from food. The lower rate of isotope uptake in mothers’ collagen relative to their offspring indicates incomplete bone collagen turnover after ten weeks. The variance of hydrogen stable isotope ratios within the same cohort may limit its usefulness as a single sample proxy for archaeological or palaeoenvironmental research.