The microbiological safety of food and the environment in which we live is currently an intensely discussed topic. Increasing production and the demand-driven global market exert pressure on ensuring sufficient high-quality food and safe drinking water. Compared to the past, increased attention in this area is now paid to important viral agents associated with food/water contaminations in both intensive research and routine diagnostics. This interest in viral agents has also increased in recent years due to the ongoing global pandemic caused by the novel coronavirus. Food- and water-borne viruses usually cause only mild and short-term diseases. The most common is gastroenteritis manifested by fever, vomiting and watery diarrhoea. However, in addition to mild febrile illness, these agents can also cause more serious conditions – respiratory infections, hepatitis, conjunctivitis, aseptic meningitis, myocarditis, encephalitis and paralysis. Globally, these diseases have significant economic impacts and are still among the leading causes of death in developing countries.This manuscript provides an overview of food- and water-borne viruses and technologies developed and currently used for their identification as causative agents. Methods for the detection of these pathogens represent an important tool for the assessment and mitigation of potential risks associated with the contamination of food and water resources. There is currently a wide range of possible approaches. Their use is differently targeted and their sensitivity, effectiveness and specificity also vary. In the case of a specific application, it is therefore necessary to choose the appropriate method, optimize it, and then verify its applicability and limits. The chosen method should be sufficiently robust, sensitive, specific and, if possible, also time and labor saving.