El presente trabajo se ha centrado en el desarrollo de emulsiones alimentarias aceite-en-agua estabilizadas con proteínas de atún. Específicamente, se ha analizado la influencia del método de conservación de las proteínas aisladas (liofilización, congelación) y de las condiciones de procesado seleccionadas sobre el comportamiento reológico y la microestructura de dichas emulsiones. Se han preparado emulsiones aceite en agua (con un contenido del 70% en peso de aceite) estabilizadas con proteínas de atún. La concentración de emulsionante usada ha sido 0,50% en peso. El comportamiento reológico de estas emulsiones no depende significativamente del método de conservación de la proteína empleado. Por otra parte, un aumento de la velocidad de agitación durante el proceso de manufactura de la emulsión da lugar a una disminución continua del tamaño medio de gota y a un aumento de las funciones viscoelásticas dinámicas, menos significativo a medida que aumenta dicha velocidad de agitación. | This work is focused on the development of o/w salad dressing-type emulsions stabilized by tuna proteins. The influence of protein conservation methods after the extraction process (freezing or liofilization) on the rheological properties and microstructure of these emulsions was analyzed. Processing variables during emulsification were also evaluated. Stable emulsions with adequate rheological and microstructural characteristics were prepared using 70% oil and 0.50% tuna proteins. From the experimental results obtained, we may conclude that emulsion rheological properties are not significantly affected by the protein conservation method selected. On the contrary, an increase in homogenization speed favours an increase in the values of the linear viscoelastic functions. Less significant is the fact that as agitation speed increases further, mean droplet size steadily decreases.

To evaluate the base line levels of potentially toxic and essential trace metals namely, Cd, Ph, Cr, Mn, Ni, Cu and Zn in water, locally grown vegetables, cereals and vegetable ghee-oil samples of a highly industrial city Gujranwala have been measured after wet digestion by employing AAS technique. Sampling was made from selected sites in and around the city over a period of three months. The concentration of Mn, Ni, Zn, Cu and Pb was found significantly higher in vegetables ranging from 3-63, 2-81, 5-92, 7- 70 and 6-60 ppm respectively. Nickel and Cu concentration was found higher in cereals whereas, Cd was found at ppb level in all variety of samples studied. Tap water, vegetable ghee-oil have recorded lower concentration of these metals. Vegetables collected from urban area have shown elevated level of contamination than those of taken from canal irrigated farms. The overall level of trace metals with few exceptions of vegetable samples was found within the permissible limits fixed by WHO. The analytical method was checked by applying on SRM of wheat and spinach. The results were found in agreement with the certified values within experimental error. The major polluting sources of the city such as automobile, man made activities, soil and road dust, metallic corrosion and industrial activities have been indicated. The environmental impacts of pollutants on water and food quality of an industrial and non-industrial city are briefly discussed in the light of their adverse effects on human health.

The formation of acrylamide during food frying is generally influenced by food type, thermal treatment and equipment. The acrylamide concentration is increased when frying oils containing a higher level of polar materials or silicone or larger amounts of diglycerides. This effect may be caused by moisture escaping from food that has an enhancing effect on the heat transfer. It was noticed that if the moisture in the frying operation was bound by special adsorbents, the acrylamide content could be reduced by more than 50 per cent. The effects of several additives like citric acid on the formation of acrylamide during frying of chips were also investigated. The mechanism of acrylamide formation in fried foods is discussed to explain these findings

The growth rates and yields of Listeria monocytogenes and Yersinia enterocolitica were determined in liquid culture media, and in model oil-in-water emulsions that contained 30, 70 or 83% (v/v) hexadecane. In emulsions with a mean droplet size of 2 pm containing 83% (v/v) hexadecane, the growth of both organisms resulted in decreased yields. Additionally, in these emulsions adjusted to pH 5.0 or 4.4 the growth rate of L. monocytogenes was significantly less than in other model systems which had an aqueous phase of equivalent chemical composition. Microscopic examination of the 83% (v/v) emulsion showed that its microstructure immobilized the bacteria, which were constrained to grow as colonies. Bacteria behaved similarly in model emulsions of either hexadecane or sunflower oil. Manipulation of the droplet size distribution of the emulsions changed the form and rate of growth of bacteria within them.