The conservation and renovation of biological diversity of territories are of vital importance in Ukraine. The concept of ecological networks has laid the foundation of these issues. In Ukraine, the law “On the ecological network” was adopted, and ecological networks were developed at the regional level. However, most developments do not fully take into account the location of reserved territories and places of concentration of valuable flora and fauna, and also often do not correspond to the principle of formation of a holistic system, which significantly reduces their environmental significance and the expediency of practical implementation. The authors of the article consider that one should estimate the natural potential of the territories in detail and objectively, as well as provide recommendations for practical actions for the renovation of biological and landscape diversity. One can do it in the process of the development of local ecological networks (at the level of separate administrative regions and territorial communities). One has developed the structure of the ecological network on the example of Stryi district of Lviv region and determined the estimation of the spatial connectivity of its crucial elements. The problem is that most of the connecting territories are formed from semi-natural and unnatural lands (hayfields, pastures, arable lands), which complicates the practical side of the project implementation. One needs to develop an effective mechanism to encourage landowners and land users to carry out environmental activities; to increase investments in re-naturalization of lands; to conduct environmental education and environmental public awareness campaign; to provide local government and other stakeholders with consulting services for the development of ecological network development programs at the local level in Ukraine.