Virus-induced gene silencing and its applications.
2008
Senthil-Kumar, M. | Anand, A. | Uppalapati, S. R. | Mysore, K. S.
Virus-induced gene silencing (VIGS) is an efficient tool for plant functional genomics. Our current understanding about the phenomenon of post-transcriptional gene silencing (PTGS) in plant defence against viruses has enabled us to modify virus genomes for VIGS in different plant species. In this review, we provide a comprehensive update on the application of VIGS. We have attempted to cover the following topics in this review: different VIGS vectors available to date; VIGS as a tool to identify novel genes involved in various aspects of plant biology; VIGS in different plant species; utility of VIGS in different plant organs; VIGS for deciphering cellular functions of known genes; and future application of VIGS in modern plant biology.
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