Development and validation of method of polymerase chain reaction in real time for detection DNA of pathogenic leptospira.
2015
UKHOVSKIY , V.; MUZYKINA, L.; GALKA, I.; SPYRYDONOV
The results of the development and validation of the method of PCR real-time for detection DNA of pathogenic leptospira by international guidelines. It was established that the developed primer hybridization system shows pronounced activity against DNA template at 45ºС the concentration of magnesium ions in the reaction mixture of 1,5 mM/microliter. Presented parameters determine the sensitivity, specificity and limits of detection techniques that suggest the possibility of its use to detect DNA of pathogenic leptospira in the diagnosis of leptospirosis in farms Ukraine
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