Histopatologi hati ayam broiler (Gallus domesticus): sebuah model forensik veteriner pada 48 jam pasca kematian | Liver histopathology of broiler chicken (Gallus domesticus): A veterinary forensic model 48 h postmortem

Indonesian. Ilmu forensik veteriner belum mendapat perhatian yang memadai di Indonesia, sehingga perlu ditingkatkan pengembangannya. Penelitian ini bertujuan mempelajari ilmu forensik veteriner melalui histopatologi hati ayam broiler 48 jam pasca kematian. Ayam broiler sebanyak 75 ekor berumur 7 hari dieutanasia dan dibagi menjadi 25 kelompok berdasarkan jam nekropsi pasca-eutanasia (n=3). Kadaver ayam dinekropsi setiap 2 jam postmortem, organ hati dikoleksi untuk dibuat potongan histopatologi dan diwarnai dengan Hematoksilin-Eosin (HE). Histopatologi hati evaluasi dan hasil yang didapat dianalisis menggunakan software ImageJ versi 1.53a. Hubungan peubah histopatologi dengan waktu postmortem dianalisis dengan metode Pearsons. Hasil penelitian menunjukkan bahwa pembusukan dimulai pada jam ke-18 post mortem yang ditandai dengan jumlah inti sel hepatosit, jam ke-20 ditemukan bakteri pembusuk, dan jam ke-22 terjadi peningkatan jarak antar hepatosit. Berdasarkan nilai korelasi Pearson, jumlah inti sel hepatosit, jarak antar hepatosit dan keberadaan bakteri pembusuk mempunyai hubungan kuat hingga sangat kuat dengan waktu postmortem sehingga hasil ini dapat menjadi parameter penentu waktu kematian (Post Mortem Interval/PMI) pada hewan.

English. Veterinary forensic science has not received adequate attention in Indonesia; therefore, its development needs to increase. This research aimed to study veterinary forensic science through histopathology of broiler chicken livers 48 h after death. Seventy-five broiler chickens aged 7 days were euthanized and divided into 25 groups based on post-euthanasia necropsy (n=3). Chicken cadavers were necropsied every 2 h postmortem, and livers were collected to prepare histopathological sections and stained with haematoxylin-eosin (HE). Liver histopathology evaluation and the results obtained were analysed using ImageJ software version 1.53a. The relationship between histopathological variables and postmortem time was analysed using the Pearson’s method. The results showed that decay began at the 18th h postmortem which was marked by the number of hepatocyte cell nuclei. At the 20th hour, putrefactive bacteria were found, and at the 22nd hour, there was an increase in the distance between the hepatocytes. Based on the Pearson correlation value, the number of hepatocyte cell nuclei, the distance between hepatocytes, and the presence of putrefactive bacteria have a strong to very strong relationship with postmortem time; therefore, these results can be used to determine the time of death (postmortem interval)