Pythium ultimum dan Phytoppythium vexans, Patogen Potensial yang Diisolasi dari Risosfer Kentang di Jawa Tengah Indonesia | Pythium ultimum and Phytopythium vexans, the Potential Pathogen Isolated from Potato Rhizosphere in Central Java, Indonesia
2022
Karmila, Miratun | Widiastuti, Ani | Wibowo, Arif | Suryanti, Suryanti
English. Phytophthora and Pythium are a group of Oomycetes that are widely associated with diseases in potato plants. Therefore, this study was conducted to identify the Oomycetes associated with the rhizosphere of infected potato plants showing leaf blight. Four isolates were collected from four regions in Central Java (UGM_St_TM, UGM_St_BK, UGM_St_BNJ, and UGM_St_KJ, the isolate from Temanggung, Bakal, Banjarnegara, Kejajar respectively), and UGM_St_NG isolate as culture collection from Laboratory of Plant Pathology. Molecular identification of all isolates was carried out using the internal transcribed spacer (ITS1/ITS4), nuclear large-ribosomal subunit (LSU), and cytochrome C oxidase subunit 1 (COX1) gene markers. Based on the results, the isolates UGM_St_TM, UGM_St_BK, and UGM_St_BNJ were identified as Phythium ultimum while UGM_St_KJ and UGM_St_NG isolate were identified as Phytopythium vexans.
Show more [+] Less [-]Indonesian. Phytophthora dan Pythium merupakan kelompok Oomycetes yang banyak berasosiasi dengan penyakit pada tanaman kentang. Penelitian ini bertujuan untuk mengidentifikasi Oomycetes yang berasosiasi dengan rizosfer kentang yang menunjukkan gejala penyakit hawar daun. Empat isolat berhasil dikoleksi dari empat wilayah sentra pertanaman kentang di Jawa Tengah (isolat UGM_St_TM, UGM_St_BK , UGM_St_BNJ , dan UGM_St_KJ berturut-turut dari Temanggung, Bakal, Banjarnegara dan Kejajar) dan satu isolat UGM_St_NG koleksi dari Laboratorium Ilmu Penyakit Tumbuhan. Identifikasi molekuler dari semua isolat dilakukan menggunakan penanda gen internal transscribed spacer (ITS1/ITS4), Nuclear large-ribosomal subunit (LSU), dan cytochrome C oxidase subunit 1 (COX1). Berdasarkan hasil penelitian, isolat UGM_St_TM, UGM_St_BK, dan UGM_St_BNJ teridentifikasi sebagai Phythium ultimum sedangkan isolat UGM_St_KJ dan UGM_St_NG teridentifikasi sebagai Phytopythium vexans.
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