Cloning of CBF3 gene from Arabidopsis thaliana stress-inducible promoter Rd29A and construction of plant expression vector | GBF3哄妞╄〃杈捐浇浣寤
2011
Zhang Chunping, Huaibei Coal Industry Teachers College, Huaibei (China) | Shu Liangzuo, Huaibei Coal Industry Teachers College, Huaibei (China) | Zhang Qijun, Jiangsu Academy of Agricultural Sciences, Nanjing (China)
[Objective] The aim was to clone CBF3 gene from Arabidopsis thaliana and construct plant expression vector pCAMBIA1301-Rd29A-CBF3. [Method] CBF3 gene and stress-inducible promoter Rd29A were amplified from the genomic DNA of A. thaliana for the construction of plant expression vector. [Result] Sequencing results showed that the cloned CBF3 gene had 750 bp, and showed 100% identity with the sequence published on GenBank. The promoter Rd29A had 1 425 bp, and showed 100% identity with the sequence published on GenBank. [Conclusion] Based on the binary vector pCAMBIA1301, the plant expression vector pCAMBIA1301-Rd29A-CBF3 was constructed successfully, which could materially improve the salt resistance, drought-tolerance, cold resistance of plants.
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