Fine mapping of resistance gene rscmvl to maize dwarf mosaic virus | 玉米矮花叶病抗病基因Rscmvl的精细定位
2011
Zhao Rongbing, Henan Agricultural University,Zhengzhou(China),College of Agronomy | Wang Yongxia, Henan Agricultural University,Zhengzhou(China),College of Agronomy | Ding Junqiang, Henan Agricultural University,Zhengzhou(China),College of Agronomy
Chinese. 摘 要：以感病亲本M017为轮回亲本、抗病亲本四一为供体亲本，构建近等基因系，对抗病基因Rscmvl进行精细定位。结果表明，在自交系四一中定位了两个抗病基因Rscmvl和Rscmv2，其中Rscmvl是抗源中普遍存在的主要抗病基因。以选育的近等基因系BC4F3、BC4F4以和BC4F5以为定位群体，在抗病基因区域内开发了9个有多态性的BAC-SSR标记，将Rscmvl定位在BAC－SSR标记A5－1和B2－1之间，其遗传距离分别为0．3cM和0．6cM，2个标记之间的物理距离为1．38Mb。
Show more [+] Less [-]English. To susceptible parent Mo17 as the recurrent parent, resistant parent SiYi as the donor parent, to self-cross on the fine mapping of resistance gene Rscmvl. The results showed that the two resistance genes Rscmvl and Rscmv2 of the resistant elite inbred line SiYi to maize dwarf Mosaic virus were identified. The near-isogenic lines of BC4F3, BC4F4 and BC4F5 Were used and 9 BAC-SSR markers with polymorphisms were developed in the region of resistance gene Rscmvl, and then Rscmvl was mapped between BAC-SSR molecular marker A5-1 and B2-1, with the genetic distances of 0.3 cM and 0.6 cM, respectively, and the physical distance between the two markers was 1.38 Mb.
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